Rapid detection and identification of Legionella pneumophila by a membrane immunoassay.

abstract：:Burkholderia pseudomallei is a saprophytic bacterium which is the causative agent of melioidosis, a common cause of fatal bacterial pneumonia and sepsis in the tropics. The incidence of melioidosis is clustered spatially and temporally and is heavily linked to rainfall and extreme weather events. Clinical case cluster...

journal_title：Applied and environmental microbiology

authors： Baker A,Tahani D,Gardiner C,Bristow KL,Greenhill AR,Warner J

更新日期：2011-10-01 00:00:00

abstract：:The restriction endonuclease Cac824I has been shown to be a major barrier to electrotransformation of Clostridium acetobutylicum ATCC 824 (L. D. Mermelstein, N. E. Welker, G. N. Bennett, and E. T. Papoutsakis, Bio/Technology 10:190-195, 1992). Methylation by the phi 3T I methyltransferase encoded by Bacillus subtilis ...

journal_title：Applied and environmental microbiology

authors： Mermelstein LD,Papoutsakis ET

更新日期：1993-04-01 00:00:00

abstract：:Salmonella enterica is an animal and zoonotic pathogen of worldwide importance. Salmonella serovars that differ in their host and tissue tropisms exist. Cattle are an important reservoir of human nontyphoidal salmonellosis, and contaminated bovine peripheral lymph nodes enter the food chain via ground beef. The relati...

journal_title：Applied and environmental microbiology

authors： Vohra P,Bugarel M,Turner F,Loneragan GH,Hope JC,Hopkins J,Stevens MP

更新日期：2018-01-31 00:00:00

abstract：:A 1.8-kb chromosomal DNA fragment of the mycoplasmalike organism (MLO) associated with apple proliferation was sequenced. Three putative open reading frames were observed on this fragment. The protein encoded by open reading frame 2 shows significant homologies with bacterial nitroreductases. From the nucleotide seque...

journal_title：Applied and environmental microbiology

authors： Jarausch W,Saillard C,Dosba F,Bové JM

更新日期：1994-08-01 00:00:00

abstract：:Two microorganisms which could degrade soybean lees efficiently were isolated and identified as Bacillus circulans and B. stearothermophilus. These two strains secreted thermostable proteases into the medium and could digest soybean lees rapidly and completely at 50 degrees C. Initially, the soybean lees were degraded...

journal_title：Applied and environmental microbiology

authors： Kubo M,Okajima J,Hasumi F

更新日期：1994-01-01 00:00:00

abstract：:A xylose reductase (XR) gene was identified from the Neurospora crassa whole-genome sequence, expressed heterologously in Escherichia coli, and purified as a His6-tagged fusion in high yield. This enzyme is one of the most active XRs thus far characterized and may be used for the in vitro production of xylitol. ...

journal_title：Applied and environmental microbiology

authors： Woodyer R,Simurdiak M,van der Donk WA,Zhao H

更新日期：2005-03-01 00:00:00

abstract：:A device for sampling at depths of up to 6,000 m is described in which 3 liters of seawater is concentrated over a Nucleopore filter to about 13 ml and retrieved under in situ pressure and temperature. Subsamples can be withdrawn into transfer units that are equipped with individual gas accumulators for preventing los...

journal_title：Applied and environmental microbiology

authors： Jannasch HW,Wirsen CO

更新日期：1977-03-01 00:00:00

abstract：:The bioconversion of sugars present in wood hemicellulose to 2,3-butanediol by Klebsiella pneumoniae grown on high sugar concentrations was investigated. When K. pneumoniae was grown under finite air conditions in the presence of added acetic acid, 50 g of D-glucose and D-xylose per liter could be converted to 25 and ...

journal_title：Applied and environmental microbiology

authors： Yu EK,Saddler JN

更新日期：1983-09-01 00:00:00

abstract：:The enzyme levanase encoded by the sacC gene from Bacillus subtilis was overexpressed in Escherichia coli with the strong, inducible tac promoter. The enzyme was purified from crude E. coli cell lysates by salting out with ammonium sulfate and chromatography on DEAE-Sepharose CL-6B, S-Sepharose, and MonoQ-Sepharose. T...

journal_title：Applied and environmental microbiology

authors： Wanker E,Huber A,Schwab H

更新日期：1995-05-01 00:00:00

abstract：:A full-scale study evaluating an inoculum addition to stimulate in situ bioremediation of oily-sludge-contaminated soil was conducted at an oil refinery where the indigenous population of hydrocarbon-degrading bacteria in the soil was very low (10(3) to 10(4) CFU/g of soil). A feasibility study was conducted prior to ...

journal_title：Applied and environmental microbiology

authors： Mishra S,Jyot J,Kuhad RC,Lal B

更新日期：2001-04-01 00:00:00

abstract：:Based on results from eight experiments, no overall difference was found between roll tube and three- and five-tube most-probable-number (MPN) methods for estimating total numbers of ruminal bacteria. However, standard errors for the replicate means within an experiment were higher with the MPN procedures. Visual grow...

journal_title：Applied and environmental microbiology

authors： Dehority BA,Tirabasso PA,Grifo AP Jr

更新日期：1989-11-01 00:00:00

abstract：:Smectite clay minerals are abundant in soils and sediments worldwide and are typically rich in Fe. While recent investigations have shown that the structural Fe(III) bound in clay minerals is reduced by microorganisms, previous studies have not tested growth with clay minerals as the sole electron acceptor. Here we ha...

journal_title：Applied and environmental microbiology

authors： Kostka JE,Dalton DD,Skelton H,Dollhopf S,Stucki JW

更新日期：2002-12-01 00:00:00

abstract：:Thirty-one urease-positive thermophilic Campylobacter (UPTC) isolates, including three reference strains (NCTC12892, NCTC12895 and NCTC12896), and three Campylobacter lari isolates, which were isolated from several countries and sources, were compared genotypically by using multilocus enzyme electrophoresis (MLEE). We...

journal_title：Applied and environmental microbiology

authors： Matsuda M,Kaneko A,Stanley T,Millar BC,Miyajima M,Murphy PG,Moore JE

更新日期：2003-06-01 00:00:00

abstract：:Two constructs derived from the alpha-amylase gene (amyA) of Lactobacillus amylovorus were expressed in Lactobacillus plantarum, and their expression products were purified, characterized, and compared. These products correspond to the complete (AmyA) and truncated (AmyADelta) forms of alpha-amylase; AmyADelta lacks t...

journal_title：Applied and environmental microbiology

authors： Rodriguez Sanoja R,Morlon-Guyot J,Jore J,Pintado J,Juge N,Guyot JP

更新日期：2000-08-01 00:00:00

abstract：:Resources from the Sinorhizobium meliloti Rm1021 open reading frame (ORF) plasmid libraries were used in a medium-throughput method to construct a set of 50 overlapping deletion mutants covering all of the Rm1021 pSymA megaplasmid except the replicon region. Each resulting pSymA derivative carried a defined deletion o...

journal_title：Applied and environmental microbiology

authors： Yurgel SN,Mortimer MW,Rice JT,Humann JL,Kahn ML

更新日期：2013-03-01 00:00:00

abstract：:The persistence of hepatitis A virus (HAV) was determined both in mixtures of septic tank effluent (STE) with dairy cattle manure slurry (DCMS) and in mixtures of STE with swine manure slurry (SMS). HAV was consistently inactivated more rapidly in the two types of mixed wastes than in STE alone or in the control Dulbe...

journal_title：Applied and environmental microbiology

authors： Deng MY,Cliver DO

更新日期：1995-01-01 00:00:00

abstract：:A new virulent phage belonging to the Siphoviridae family and able to infect Lactococcus garvieae strains was isolated from compost soil. Phage GE1 has a prolate capsid (56 by 38 nm) and a long noncontractile tail (123 nm). It had a burst size of 139 and a latent period of 31 min. Its host range was limited to only tw...

journal_title：Applied and environmental microbiology

authors： Eraclio G,Tremblay DM,Lacelle-Côté A,Labrie SJ,Fortina MG,Moineau S

更新日期：2015-12-01 00:00:00

abstract：:After propagation of Rhizopus javanicus in defined media containing glucose, urea, and mineral salts in deionized distilled water, the ability of the nonliving biomass to sequester cupric ion was assayed. Growth, uptake capacity (saturation uptake at >1 mM Cu concentration in solution), and biosorptive yield (biomass ...

journal_title：Applied and environmental microbiology

authors： Treen-Sears ME,Martin SM,Volesky B

更新日期：1984-07-01 00:00:00

abstract：:Bacteria, yeasts, and molds isolated from partially processed iceberg lettuce were taxonomically classified. The majority of bacterial isolates were gram-negative rods. Pseudomonas, Erwinia, and Serratia species were commonly found. Yeasts most frequently isolated from lettuce included members of the genera Candida, C...

journal_title：Applied and environmental microbiology

authors： Magnuson JA,King AD Jr,Török T

更新日期：1990-12-01 00:00:00

abstract：:The ability of microorganisms to perform dissimilatory (per)chlorate reduction is, for most species, known to be oxygen sensitive. Consequently, bioremediation processes for the removal of oxochlorates will be disturbed if oxygen is present. We measured the expression of chlorite dismutase and chlorate reductase in th...

journal_title：Applied and environmental microbiology

authors： Lindqvist MH,Johansson N,Nilsson T,Rova M

更新日期：2012-06-01 00:00:00

abstract：:Blooms of the cyanobacterium Anabaena circinalis are a major worldwide problem due to their production of a range of toxins, in particular the neurotoxins anatoxin-a and paralytic shellfish poisons (PSPs). Although there is a worldwide distribution of A. circinalis, there is a geographical segregation of neurotoxin pr...

journal_title：Applied and environmental microbiology

authors： Beltran EC,Neilan BA

更新日期：2000-10-01 00:00:00

abstract：:The vaginal mucosa of ovariectomized female rats has been examined by scanning electron microscopy before and after estrogen [1,3,5(10)-estratriene-3,17beta-diol] treatment. Without estrogen stimulation vaginal colonization is minimal and the epithelium is characterized by a layer of epithelial cells covered with smal...

journal_title：Applied and environmental microbiology

authors： Larsen B,Markovetz AJ,Galask RP

更新日期：1977-07-01 00:00:00

abstract：:Erysipelothrix rhusiopathiae causes swine erysipelas, an important infectious disease in the swine industry. In Japan, the incidence of acute swine erysipelas due to E. rhusiopathiae serovar 1a has recently increased markedly. To study the genetic relatedness of the strains from the recent cases, we analyzed 34 E. rhu...

journal_title：Applied and environmental microbiology

authors： Ogawa Y,Shiraiwa K,Ogura Y,Ooka T,Nishikawa S,Eguchi M,Hayashi T,Shimoji Y

更新日期：2017-05-17 00:00:00

abstract：:The kinetics of ethanol inhibition on cell growth and ethanol production by Kluyveromyces marxianus UCD (FST) 55-82 were studied during batch growth. The liquid medium contained 10% (wt/vol) inulin-type sugars derived from an extract of Jerusalem artichoke (Helianthus tuberosus) tubers, supplemented with small amounts...

journal_title：Applied and environmental microbiology

authors： Bajpai P,Margaritis A

更新日期：1982-12-01 00:00:00

abstract：:A mathematical model was developed to estimate the number of logarithmic cycles (LDec) of lipopolysaccharide concentration destroyed by a dry-heat sterilization process. The LDec values calculated from the mathematical model agreed well with those obtained from the destruction of lipopolysaccharide by a dry-heat treat...

journal_title：Applied and environmental microbiology

authors： Tsuji K,Lewis AR

更新日期：1978-11-01 00:00:00

abstract：:Identification and design of new cellulolytic enzymes with higher catalytic efficiency are a key factor in reducing the production cost of lignocellulosic bioalcohol. We report here identification of a novel β-glucosidase (Gluc1C) from Paenibacillus sp. strain MTCC 5639 and construction of bifunctional chimeric protei...

journal_title：Applied and environmental microbiology

authors： Adlakha N,Sawant S,Anil A,Lali A,Yazdani SS

更新日期：2012-10-01 00:00:00

abstract：:We present a phylogenetic analysis of nine strains of symbiotic nitrogen-fixing bacteria isolated from nodules of tagasaste (Chamaecytisus proliferus) and other endemic woody legumes of the Canary Islands, Spain. These and several reference strains were characterized genotypically at different levels of taxonomic reso...

journal_title：Applied and environmental microbiology

authors： Vinuesa P,Rademaker JL,de Bruijn FJ,Werner D

更新日期：1998-06-01 00:00:00

abstract：:Separating dietary fiber from other polysaccharides in digesta and feces is necessary to understand its mechanisms of action. A gravimetric method that separates fecal plant and bacterial matter based on size and density was evaluated and modified to determine the plant and bacterial mass of lyophilized whole and blen...

journal_title：Applied and environmental microbiology

authors： Cabotaje LM,López-Guisa JM,Shinnick FL,Marlett JA

更新日期：1990-06-01 00:00:00

abstract：:A variant of Bacillus thuringiensis subsp. kurstaki containing a single, stable copy of a uniquely amplifiable DNA oligomer integrated into the genome for tracking the fate of biological agents in the environment was developed. The use of genetically tagged spores overcomes the ambiguity of discerning the test materia...

journal_title：Applied and environmental microbiology

authors： Emanuel PA,Buckley PE,Sutton TA,Edmonds JM,Bailey AM,Rivers BA,Kim MH,Ginley WJ,Keiser CC,Doherty RW,Kragl FJ,Narayanan FE,Katoski SE,Paikoff S,Leppert SP,Strawbridge JB,VanReenen DR,Biberos SS,Moore D,Phillips DW,

更新日期：2012-12-01 00:00:00

abstract：:The fate of selenium in the environment is controlled, in part, by microbial selenium oxyanion reduction and Se(0) precipitation. In this study, we identified a genetic regulator that controls selenate reductase activity in the Se-reducing bacterium Enterobacter cloacae SLD1a-1. Heterologous expression of the global a...

journal_title：Applied and environmental microbiology

authors： Yee N,Ma J,Dalia A,Boonfueng T,Kobayashi DY

更新日期：2007-03-01 00:00:00