Comparative characterization of complete and truncated forms of Lactobacillus amylovorus alpha-amylase and role of the C-terminal direct repeats in raw-starch binding.

abstract：:This study aimed at assessing the dynamics of lactic acid bacteria and other Firmicutes associated with durum wheat organs and processed products. 16S rRNA gene-based high-throughput sequencing showed that Lactobacillus, Streptococcus, Enterococcus, and Lactococcus were the main epiphytic and endophytic genera among l...

journal_title：Applied and environmental microbiology

authors： Minervini F,Celano G,Lattanzi A,Tedone L,De Mastro G,Gobbetti M,De Angelis M

更新日期：2015-10-01 00:00:00

abstract：:Escherichia coli offers unparalleled engineering capacity in the context of heterologous natural product biosynthesis. However, as with other heterologous hosts, cellular metabolism must be designed or redesigned to support final compound formation. This task is at once complicated and aided by the fact that the cell ...

journal_title：Applied and environmental microbiology

authors： Jiang M,Stephanopoulos G,Pfeifer BA

更新日期：2012-04-01 00:00:00

abstract：:The genus Shewanella is well known for its genetic diversity, its outstanding respiratory capacity, and its high potential for bioremediation. Here, a novel strain isolated from sediments of the Indian Ocean was characterized. A 16S rRNA analysis indicated that it belongs to the species Shewanella decolorationis It wa...

journal_title：Applied and environmental microbiology

authors： Lemaire ON,Honoré FA,Tempel S,Fortier EM,Leimkühler S,Méjean V,Iobbi-Nivol C

更新日期：2019-08-29 00:00:00

abstract：:The acid-insoluble metal sulfides FeS2, MoS2, and WS2 are chemically attacked by iron(III) hexahydrate ions, generating thiosulfate, which is oxidized to sulfuric acid. Other metal sulfides are attacked by iron(III) ions and by protons, resulting in the formation of elemental sulfur via intermediary polysulfides. Sulf...

journal_title：Applied and environmental microbiology

authors： Schippers A,Sand W

更新日期：1999-01-01 00:00:00

abstract：:Aminopolycarboxylic acids are ubiquitous in natural waters and wastewaters. They have the ability to form very stable water-soluble complexes with many metallic di- or trivalent ions. The iron complex nitrilotriacetic acid-Fe(III) (FeNTA) has been previously shown to increase drastically the rate of photo- and biodegr...

journal_title：Applied and environmental microbiology

authors： Bunescu A,Besse-Hoggan P,Sancelme M,Mailhot G,Delort AM

更新日期：2008-10-01 00:00:00

abstract：:Recently, a new metabolic link between fatty acid de novo biosynthesis and biosynthesis of poly(3-hydroxy-alkanoate) consisting of medium-chain-length constituents (C(6) to C(14)) (PHA(MCL)), catalyzed by the 3-hydroxydecanoyl-[acyl-carrier-protein]:CoA transacylase (PhaG), has been identified in Pseudomonas putida (B...

journal_title：Applied and environmental microbiology

authors： Fiedler S,Steinbüchel A,Rehm BH

更新日期：2000-05-01 00:00:00

abstract：:Factors affecting natural transformation of Acinetobacter calcoaceticus BD413 with homologous chromosomal DNA in a silt loam soil microcosm were investigated. Inducible transformation of declining populations of noncompetent A. calcoaceticus cells was detectable for up to 6 days when a simple carbon source, salts, and...

journal_title：Applied and environmental microbiology

authors： Nielsen KM,Bones AM,Van Elsas JD

更新日期：1997-10-01 00:00:00

abstract：:An examination was made of various sites along the length of the swine large intestine, using strictly anaerobic culture methods. Sites were separated by differential washing into fractions described as lumenal content, lumenal surface layer, and intestinal wall tissue. Direct microscopic clump counts averaged 13.3 x ...

journal_title：Applied and environmental microbiology

authors： Russell EG

更新日期：1979-02-01 00:00:00

abstract：:Studies of phytoplasma-insect vector interactions and epidemiological surveys of plant yellows associated with the stolbur phytoplasma (StolP) require the identification of relevant candidate genes and typing markers. A recent StolP genome survey identified a partial coding sequence, SR01H10, having no homologue in th...

journal_title：Applied and environmental microbiology

authors： Cimerman A,Pacifico D,Salar P,Marzachì C,Foissac X

更新日期：2009-05-01 00:00:00

abstract：:Root-colonizing, saprophytic fluorescent pseudomonads of the Pseudomonas putida-P. fluorescens group express similar levels of catalase and superoxide dismutase activities during growth on a sucrose- and amino acid-rich medium. Increased specific activities of catalase but not superoxide dismutase were observed during...

journal_title：Applied and environmental microbiology

authors： Katsuwon J,Anderson AJ

更新日期：1990-11-01 00:00:00

abstract：:Total coliforms and total coliforms resistant to streptomycin, tetracycline, or chloramphenicol were isolated from filtered activated sludge effluents before and after UV light irradiation. Although the UV irradiation effectively disinfected the wastewater effluent, the percentage of the total surviving coliform popul...

journal_title：Applied and environmental microbiology

authors： Meckes MC

更新日期：1982-02-01 00:00:00

abstract：:The soybean is an exotic plant introduced in Paraguay in this century; commercial cropping expanded after the 1970s. Inoculation is practiced in just 15 to 20% of the cropping areas, but root nodulation occurs in most sites where soybeans grow. Little is known about rhizobial diversity in South America, and no study h...

journal_title：Applied and environmental microbiology

authors： Chen LS,Figueredo A,Pedrosa FO,Hungria M

更新日期：2000-11-01 00:00:00

abstract：:Thermophilic sulfate-reducing bacteria were enriched from samples obtained from a geothermal underground mine in Japan. The enrichment cultures contained bacteria affiliated with the genera Desulfotomaculum, Thermanaeromonas, Thermincola, Thermovenabulum, Moorella, "Natronoanaerobium," and Clostridium. Two novel therm...

journal_title：Applied and environmental microbiology

authors： Kaksonen AH,Plumb JJ,Robertson WJ,Spring S,Schumann P,Franzmann PD,Puhakka JA

更新日期：2006-05-01 00:00:00

abstract：:The subsurface of a tidal-flat sediment was analyzed down to 360 cm in depth by molecular and geochemical methods. A community structure analysis of all three domains of life was performed using domain-specific PCR followed by denaturing gradient gel electrophoresis analysis and sequencing of characteristic bands. The...

journal_title：Applied and environmental microbiology

authors： Wilms R,Sass H,Köpke B,Köster J,Cypionka H,Engelen B

更新日期：2006-04-01 00:00:00

abstract：:A xylose reductase (XR) gene was identified from the Neurospora crassa whole-genome sequence, expressed heterologously in Escherichia coli, and purified as a His6-tagged fusion in high yield. This enzyme is one of the most active XRs thus far characterized and may be used for the in vitro production of xylitol. ...

journal_title：Applied and environmental microbiology

authors： Woodyer R,Simurdiak M,van der Donk WA,Zhao H

更新日期：2005-03-01 00:00:00

abstract：:Production of bacteriocin activity designated pediocin PA-1 was associated with the presence of a 6.2-megadalton plasmid in Pediococcus acidilactici PAC1.0. The bacteriocin exhibited activity against P. acidilactici, P. pentosaceus, Lactobacillus plantarum, L. casei, L. bifermentans, and Leuconostoc mesenteroides subs...

journal_title：Applied and environmental microbiology

authors： Gonzalez CF,Kunka BS

更新日期：1987-10-01 00:00:00

abstract：:The DNA sequence of a 359-bp fragment of nifH was determined for the heterocystous strains Anabaena sp. strain CA (ATCC 33047), Nostoc muscorum UTEX 1933, a Nostoc sp., Gloeothece sp. strain ATCC 27152, Lyngbya lagerheimii UTEX 1930, and Plectonema boryanum IU 594. Results confirmed that the DNA sequence of the 359-bp...

journal_title：Applied and environmental microbiology

authors： Ben-Porath J,Zehr JP

更新日期：1994-03-01 00:00:00

abstract：:The pathways for degradation of aromatic hydrocarbons are constantly modified by a variety of genetic mechanisms. Genetic studies carried out with Pseudomonas stutzeri OX1 suggested that the tou operon coding for toluene o-xylene monooxygenase (ToMO) was recently recruited into a preexisting pathway that already posse...

journal_title：Applied and environmental microbiology

authors： Cafaro V,Notomista E,Capasso P,Di Donato A

更新日期：2005-08-01 00:00:00

abstract：:Microbial bioremediation is a promising approach for the removal of polycyclic aromatic hydrocarbon (PAH) contaminants. Many degraders of PAHs possess efflux pump genes in their genomes; however, their specific roles in the degradation of PAHs have not been clearly elucidated. In this study, two efflux pumps, TtgABC a...

journal_title：Applied and environmental microbiology

authors： Yao X,Tao F,Zhang K,Tang H,Xu P

更新日期：2017-12-01 00:00:00

abstract：:Resistance to desiccation was utilized in the selection of highly radiation-resistant asporogenous bacteria from non-irradiated sources. A bacterial suspension in phosphate buffer was dried in a thin film at 25 degrees C and 33% relative humidity. Storage under these conditions for 15 days or more reduced the number o...

journal_title：Applied and environmental microbiology

authors： Sanders SW,Maxcy RB

更新日期：1979-09-01 00:00:00

abstract：:The extensive use of antibiotics in hospitals and in the animal breeding industry has promoted antibiotic resistance in bacteria, which resulted in the emergence of a large number of antibiotic resistance genes in the intestinal tract of human and farmed animals. Genetic exchange of resistance genes between the two ec...

journal_title：Applied and environmental microbiology

authors： Wang C,Song Y,Tang N,Zhang G,Leclercq SO,Feng J

更新日期：2020-12-11 00:00:00

abstract：:A human feeding study was performed with Lactococcus lactis TC165.5, which is genetically marked by insertion of the sucrose-nisin conjugative transposon Tn5276 and chromosomal resistance to rifampin and streptomycin. The fate of strain TC165.5 and its nucleic acids was monitored by conventional plating methods and by...

journal_title：Applied and environmental microbiology

authors： Klijn N,Weerkamp AH,de Vos WM

更新日期：1995-07-01 00:00:00

abstract：:Intact core tetraether membrane lipids of marine planktonic Crenarchaeota were quantified in water column-suspended particulate matter obtained from four depth intervals ( approximately 70, 500, 1,000 and 1,500 m) at seven stations in the northwestern Arabian Sea to investigate the distribution of the organisms at var...

journal_title：Applied and environmental microbiology

authors： Sinninghe Damsté JS,Rijpstra WI,Hopmans EC,Prahl FG,Wakeham SG,Schouten S

更新日期：2002-06-01 00:00:00

abstract：:A novel enterotoxin gene was cloned from Bacillus cereus FM1, and its nucleotide sequence was determined. Previously, a 45-kDa protein causing characteristic enterotoxin symptoms in higher animals had been isolated (K. Shinagawa, p. 181-193, in A. E. Pohland et al., ed., Microbial Toxins in Foods and Feeds, 1990) from...

journal_title：Applied and environmental microbiology

authors： Asano SI,Nukumizu Y,Bando H,Iizuka T,Yamamoto T

更新日期：1997-03-01 00:00:00

abstract：:The thermophilic bacterium Bacillus thermoantarcticus produces two exocellular polysaccharides (EPS 1 and EPS 2), which can be obtained from the supernatant of liquid cultures by cold-ethanol precipitation, in yields as high as 400 mg liter(sup-1). The EPS fraction was produced with all substrates tested, although a h...

journal_title：Applied and environmental microbiology

authors： Manca MC,Lama L,Improta R,Esposito E,Gambacorta A,Nicolaus B

更新日期：1996-09-01 00:00:00

abstract：:Source attribution studies report that the consumption of contaminated poultry is the primary source for acquiring human campylobacteriosis. Oral administration of an engineered Escherichia coli strain expressing the Campylobacter jejuni N-glycan reduces bacterial colonization in specific-pathogen-free leghorn chicken...

journal_title：Applied and environmental microbiology

authors： Nothaft H,Perez-Muñoz ME,Gouveia GJ,Duar RM,Wanford JJ,Lango-Scholey L,Panagos CG,Srithayakumar V,Plastow GS,Coros C,Bayliss CD,Edison AS,Walter J,Szymanski CM

更新日期：2017-11-16 00:00:00

abstract：:The effective flux between phospholipids and neutral lipids is critical for a high level of biosynthesis and accumulation of very-long-chain polyunsaturated fatty acids (VLCPUFAs), such as arachidonic acid (ARA; 20:4n-6), eicosapentaenoic acid (EPA; 20:5n-3), and docosahexaenoic acid (DHA; 22:6n-3). Here we describe a...

journal_title：Applied and environmental microbiology

authors： Chen Y,Chi HY,Meesapyodsuk D,Qiu X

更新日期：2013-03-01 00:00:00

abstract：:The growth characteristics and acetate production of several Escherichia coli strains were compared by using shake flasks, batch fermentations, and glucose-feedback-controlled fed-batch fermentations to assess the potential of each strain to grow at high cell densities. Of the E. coli strains tested, including JM105, ...

journal_title：Applied and environmental microbiology

authors： Luli GW,Strohl WR

更新日期：1990-04-01 00:00:00

abstract：:Recombinant DNA clones carrying high-copy or low-copy sequences from Aspergillus nidulans and Neurospora crassa were used to identify restriction fragment length polymorphisms (RFLPs) diagnostic for members of the A. flavus group: A. flavus, A. parasiticus, and A. nomius. These fungi were resolved into three distinct ...

journal_title：Applied and environmental microbiology

authors： Moody SF,Tyler BM

更新日期：1990-08-01 00:00:00

abstract：:Although stimulation of dissimilatory metal reduction to promote the reductive precipitation of uranium has been shown to successfully remove uranium from some aquifer sediments, the organisms in the family Geobacteraceae that have been found to be associated with metal reduction in previous studies are not known to g...

journal_title：Applied and environmental microbiology

authors： Nevin KP,Finneran KT,Lovley DR

更新日期：2003-06-01 00:00:00