Abstract:
:Source attribution studies report that the consumption of contaminated poultry is the primary source for acquiring human campylobacteriosis. Oral administration of an engineered Escherichia coli strain expressing the Campylobacter jejuni N-glycan reduces bacterial colonization in specific-pathogen-free leghorn chickens, but only a fraction of birds respond to vaccination. Optimization of the vaccine for commercial broiler chickens has great potential to prevent the entry of the pathogen into the food chain. Here, we tested the same vaccination approach in broiler chickens and observed similar efficacies in pathogen load reduction, stimulation of the host IgY response, the lack of C. jejuni resistance development, uniformity in microbial gut composition, and the bimodal response to treatment. Gut microbiota analysis of leghorn and broiler vaccine responders identified one member of Clostridiales cluster XIVa, Anaerosporobacter mobilis, that was significantly more abundant in responder birds. In broiler chickens, coadministration of the live vaccine with A. mobilis or Lactobacillus reuteri, a commonly used probiotic, resulted in increased vaccine efficacy, antibody responses, and weight gain. To investigate whether the responder-nonresponder effect was due to the selection of a C. jejuni "supercolonizer mutant" with altered phase-variable genes, we analyzed all poly(G)-containing loci of the input strain compared to nonresponder colony isolates and found no evidence of phase state selection. However, untargeted nuclear magnetic resonance (NMR)-based metabolomics identified a potential biomarker negatively correlated with C. jejuni colonization levels that is possibly linked to increased microbial diversity in this subgroup. The comprehensive methods used to examine the bimodality of the vaccine response provide several opportunities to improve the C. jejuni vaccine and the efficacy of any vaccination strategy.IMPORTANCECampylobacter jejuni is a common cause of human diarrheal disease worldwide and is listed by the World Health Organization as a high-priority pathogen. C. jejuni infection typically occurs through the ingestion of contaminated chicken meat, so many efforts are targeted at reducing C. jejuni levels at the source. We previously developed a vaccine that reduces C. jejuni levels in egg-laying chickens. In this study, we improved vaccine performance in meat birds by supplementing the vaccine with probiotics. In addition, we demonstrated that C. jejuni colonization levels in chickens are negatively correlated with the abundance of clostridia, another group of common gut microbes. We describe new methods for vaccine optimization that will assist in improving the C. jejuni vaccine and other vaccines under development.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Nothaft H,Perez-Muñoz ME,Gouveia GJ,Duar RM,Wanford JJ,Lango-Scholey L,Panagos CG,Srithayakumar V,Plastow GS,Coros C,Bayliss CD,Edison AS,Walter J,Szymanski CMdoi
10.1128/AEM.01523-17subject
Has Abstractpub_date
2017-11-16 00:00:00issue
23eissn
0099-2240issn
1098-5336pii
AEM.01523-17journal_volume
83pub_type
杂志文章abstract::So far, only scarce and rather diffuse information is available on bacteriophages infecting members of the genus Bifidobacterium. In the current study, we investigated the genetic organization, phylogenetic relationships, and, in some cases, transcription profiles and inducibility of 19 prophage-like elements present ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01112-09
更新日期:2009-11-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/aem.66.4.1479-1488.2000
更新日期:2000-04-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.62.9.3470-3473.1996
更新日期:1996-09-01 00:00:00
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更新日期:2015-06-01 00:00:00
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pub_type: 杂志文章
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更新日期:1982-10-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.58.11.3580-3585.1992
更新日期:1992-11-01 00:00:00
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更新日期:2020-11-10 00:00:00
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更新日期:1982-08-01 00:00:00
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pub_type: 杂志文章
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更新日期:2014-01-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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更新日期:1986-04-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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更新日期:1988-11-01 00:00:00
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pub_type: 杂志文章
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更新日期:2015-11-13 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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更新日期:1981-06-01 00:00:00
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更新日期:2006-06-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/AEM.00616-20
更新日期:2020-08-18 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.58.7.2321-2325.1992
更新日期:1992-07-01 00:00:00
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更新日期:2006-10-01 00:00:00
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pub_type: 杂志文章
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更新日期:2012-05-01 00:00:00
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journal_title:Applied and environmental microbiology
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doi:10.1128/AEM.53.10.2548-2553.1987
更新日期:1987-10-01 00:00:00
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更新日期:1997-09-01 00:00:00
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更新日期:2018-08-31 00:00:00
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更新日期:2001-06-01 00:00:00
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journal_title:Applied and environmental microbiology
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更新日期:2001-11-01 00:00:00
abstract::2-Methyl-6-ethylaniline (MEA) is the main microbial degradation intermediate of the chloroacetanilide herbicides acetochlor and metolachlor. Sphingobium sp. strain MEA3-1 can utilize MEA and various alkyl-substituted aniline and phenol compounds as sole carbon and energy sources for growth. We isolated the mutant stra...
journal_title:Applied and environmental microbiology
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更新日期:2015-12-01 00:00:00
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journal_title:Applied and environmental microbiology
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更新日期:2010-10-01 00:00:00
abstract::To gain a predictive understanding of the interspecies interactions within microbial communities that govern community function, the genomic complement of every member population must be determined. Although metagenomic sequencing has enabled the de novo reconstruction of some microbial genomes from environmental comm...
journal_title:Applied and environmental microbiology
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更新日期:2015-10-23 00:00:00
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journal_title:Applied and environmental microbiology
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更新日期:2017-02-15 00:00:00
abstract::Our purpose was to develop a rapid, inexpensive method of diagnosing the source of fecal pollution in water. In previous research, we identified Bacteroides-Prevotella ribosomal DNA (rDNA) PCR markers based on analysis. These markers length heterogeneity PCR and terminal restriction fragment length polymorphism distin...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.66.10.4571-4574.2000
更新日期:2000-10-01 00:00:00
abstract::The cyanophycin (CGP) synthetase gene (cphANE1) of the transposon-induced argL mutant NE1 of the cyanobacterium Nostoc ellipsosporum, which exhibits a CGP-leaky phenotype during diazotrophical growth, was cloned and expressed in Escherichia coli strain TOP10. Its amino acid sequence exhibited high similarities to CphA...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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更新日期:2006-12-01 00:00:00