Coadministration of the Campylobacter jejuni N-Glycan-Based Vaccine with Probiotics Improves Vaccine Performance in Broiler Chickens.

Abstract:

:Source attribution studies report that the consumption of contaminated poultry is the primary source for acquiring human campylobacteriosis. Oral administration of an engineered Escherichia coli strain expressing the Campylobacter jejuni N-glycan reduces bacterial colonization in specific-pathogen-free leghorn chickens, but only a fraction of birds respond to vaccination. Optimization of the vaccine for commercial broiler chickens has great potential to prevent the entry of the pathogen into the food chain. Here, we tested the same vaccination approach in broiler chickens and observed similar efficacies in pathogen load reduction, stimulation of the host IgY response, the lack of C. jejuni resistance development, uniformity in microbial gut composition, and the bimodal response to treatment. Gut microbiota analysis of leghorn and broiler vaccine responders identified one member of Clostridiales cluster XIVa, Anaerosporobacter mobilis, that was significantly more abundant in responder birds. In broiler chickens, coadministration of the live vaccine with A. mobilis or Lactobacillus reuteri, a commonly used probiotic, resulted in increased vaccine efficacy, antibody responses, and weight gain. To investigate whether the responder-nonresponder effect was due to the selection of a C. jejuni "supercolonizer mutant" with altered phase-variable genes, we analyzed all poly(G)-containing loci of the input strain compared to nonresponder colony isolates and found no evidence of phase state selection. However, untargeted nuclear magnetic resonance (NMR)-based metabolomics identified a potential biomarker negatively correlated with C. jejuni colonization levels that is possibly linked to increased microbial diversity in this subgroup. The comprehensive methods used to examine the bimodality of the vaccine response provide several opportunities to improve the C. jejuni vaccine and the efficacy of any vaccination strategy.IMPORTANCECampylobacter jejuni is a common cause of human diarrheal disease worldwide and is listed by the World Health Organization as a high-priority pathogen. C. jejuni infection typically occurs through the ingestion of contaminated chicken meat, so many efforts are targeted at reducing C. jejuni levels at the source. We previously developed a vaccine that reduces C. jejuni levels in egg-laying chickens. In this study, we improved vaccine performance in meat birds by supplementing the vaccine with probiotics. In addition, we demonstrated that C. jejuni colonization levels in chickens are negatively correlated with the abundance of clostridia, another group of common gut microbes. We describe new methods for vaccine optimization that will assist in improving the C. jejuni vaccine and other vaccines under development.

journal_name

Appl Environ Microbiol

authors

Nothaft H,Perez-Muñoz ME,Gouveia GJ,Duar RM,Wanford JJ,Lango-Scholey L,Panagos CG,Srithayakumar V,Plastow GS,Coros C,Bayliss CD,Edison AS,Walter J,Szymanski CM

doi

10.1128/AEM.01523-17

subject

Has Abstract

pub_date

2017-11-16 00:00:00

issue

23

eissn

0099-2240

issn

1098-5336

pii

AEM.01523-17

journal_volume

83

pub_type

杂志文章
  • Comparative analyses of prophage-like elements present in bifidobacterial genomes.

    abstract::So far, only scarce and rather diffuse information is available on bacteriophages infecting members of the genus Bifidobacterium. In the current study, we investigated the genetic organization, phylogenetic relationships, and, in some cases, transcription profiles and inducibility of 19 prophage-like elements present ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01112-09

    authors: Ventura M,Turroni F,Lima-Mendez G,Foroni E,Zomer A,Duranti S,Giubellini V,Bottacini F,Horvath P,Barrangou R,Sela DA,Mills DA,van Sinderen D

    更新日期:2009-11-01 00:00:00

  • Sediment microbial community structure and mercury methylation in mercury-polluted Clear Lake, California.

    abstract::Spatial and temporal variations in sediment microbial community structure in a eutrophic lake polluted with inorganic mercury were identified using polar lipid fatty acid (PLFA) analysis. Microbial community structure was strongly related to mercury methylation potential, sediment organic carbon content, and lake loca...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.66.4.1479-1488.2000

    authors: Macalady JL,Mack EE,Nelson DC,Scow KM

    更新日期:2000-04-01 00:00:00

  • Elution of viruses by ionic and nonionic surfactants.

    abstract::The ionic and nonionic surfactants sodium dodecyl sulfate and Triton X-100, respectively, eluted two viruses, phi X174 and PRD1, which were adsorbed to the ionic and nonionic binding membranes cationic polysulfone and nitrocellulose, respectively. Results indicated that complete elution was readily achieved only when ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.62.9.3470-3473.1996

    authors: Fujito BT,Lytle CD

    更新日期:1996-09-01 00:00:00

  • Polyphenolic extract from maple syrup potentiates antibiotic susceptibility and reduces biofilm formation of pathogenic bacteria.

    abstract::Phenolic compounds are believed to be promising candidates as complementary therapeutics. Maple syrup, prepared by concentrating the sap from the North American maple tree, is a rich source of natural and process-derived phenolic compounds. In this work, we report the antimicrobial activity of a phenolic-rich maple sy...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00239-15

    authors: Maisuria VB,Hosseinidoust Z,Tufenkji N

    更新日期:2015-06-01 00:00:00

  • Genetic (In)stability of 2,6-Dichlorobenzamide Catabolism in Aminobacter sp. Strain MSH1 Biofilms under Carbon Starvation Conditions.

    abstract::Aminobacter sp. strain MSH1 grows on and mineralizes the groundwater micropollutant 2,6-dichlorobenzamide (BAM) and is of interest for BAM removal in drinking water treatment plants (DWTPs). The BAM-catabolic genes in MSH1 are located on plasmid pBAM1, carrying bbdA, which encodes the conversion of BAM to 2,6-dichloro...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00137-17

    authors: Horemans B,Raes B,Brocatus H,T'Syen J,Rombouts C,Vanhaecke L,Hofkens J,Springael D

    更新日期:2017-05-17 00:00:00

  • Secalonic acid D: natural contaminant of corn dust.

    abstract::The mycotoxin secalonic acid D was detected for the first time as a natural contaminant of corn dust obtained from grain storage elevators. Secalonic acid D amounts ranged from 0.3 to 4.5 ppm (0.3 to 4.5 micrograms/g), as determined by reversed-phase high-performance liquid chromatography. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.44.4.1007-1008.1982

    authors: Ehrlich KC,Lee LS,Ciegler A,Palmgren MS

    更新日期:1982-10-01 00:00:00

  • The anaerobic degradation of 3-chloro-4-hydroxybenzoate in freshwater sediment proceeds via either chlorophenol or hydroxybenzoate to phenol and subsequently to benzoate.

    abstract::To study the anaerobic degradation of the chimera 3-chloro-4-hydroxybenzoate (3-Cl,4-OHB), anaerobic freshwater sediment samples from the vicinity of Athens, Ga., were adapted for the transformation of 4-hydroxybenzoate (4-OHB), 3-chlorobenzoate (3-CB), 2-chlorophenol (2-CP), and 2,4-dichlorophenol (2,4-DCP). In nonad...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.58.11.3580-3585.1992

    authors: Zhang X,Wiegel J

    更新日期:1992-11-01 00:00:00

  • Metabolic Diversity and Evolutionary History of the Archaeal Phylum "Candidatus Micrarchaeota" Uncovered from a Freshwater Lake Metagenome.

    abstract::Acidophilic archaea of the archaeal Richmond Mine acidophilic nanoorganisms (ARMAN) group from the uncultured candidate phylum "Candidatus Micrarchaeota" have small genomes and cell sizes and are known to be metabolically dependent and physically associated with their Thermoplasmatales hosts. However, phylogenetically...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02199-20

    authors: Kadnikov VV,Savvichev AS,Mardanov AV,Beletsky AV,Chupakov AV,Kokryatskaya NM,Pimenov NV,Ravin NV

    更新日期:2020-11-10 00:00:00

  • Effects of surface area and flow rate on marine bacterial growth in activated carbon columns.

    abstract::The colonization of granular activated carbon columns by bacteria can have both beneficial and potentially detrimental consequences. Bacterial growth on the carbon surface can remove adsorbed organics and thus partially regenerate the carbon bed. However, growth can also increase the levels of bacteria in the column e...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.44.2.471-477.1982

    authors: Shimp RJ,Pfaender FK

    更新日期:1982-08-01 00:00:00

  • Response of free-living nitrogen-fixing microorganisms to land use change in the Amazon rainforest.

    abstract::The Amazon rainforest, the largest equatorial forest in the world, is being cleared for pasture and agricultural use at alarming rates. Tropical deforestation is known to cause alterations in microbial communities at taxonomic and phylogenetic levels, but it is unclear whether microbial functional groups are altered. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02362-13

    authors: Mirza BS,Potisap C,Nüsslein K,Bohannan BJ,Rodrigues JL

    更新日期:2014-01-01 00:00:00

  • Evaluation of culture media for recovery of Staphylococcus aureus from swimming pools.

    abstract::Several selective media were evaluated for the primary isolation and enumeration of Staphylococcus aureus from halogenated indoor swimming pool waters. Standard plate counts of the viable population and total coliform densities were also determined to ascertain their value as indicator systems. All studies were done w...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.51.4.699-702.1986

    authors: Alico RK,Dragonjac MF

    更新日期:1986-04-01 00:00:00

  • Procedure for isolating the endophyte from tall fescue and screening isolates for ergot alkaloids.

    abstract::A procedure was developed to isolate and determine ergot alkaloid production by Acremonium coenophialum, the endophytic fungus of tall fescue. The procedure established that macerated leaf sheath or pith from inflorescence stem placed either in a liquid medium or on a corn meal-malt extract agar medium produced isolat...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.54.11.2615-2618.1988

    authors: Bacon CW

    更新日期:1988-11-01 00:00:00

  • Pectin and Xyloglucan Influence the Attachment of Salmonella enterica and Listeria monocytogenes to Bacterial Cellulose-Derived Plant Cell Wall Models.

    abstract::Minimally processed fresh produce has been implicated as a major source of foodborne microbial pathogens globally. These pathogens must attach to the produce in order to be transmitted. Cut surfaces of produce that expose cell walls are particularly vulnerable. Little is known about the roles that different structural...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02609-15

    authors: Tan MS,Rahman S,Dykes GA

    更新日期:2015-11-13 00:00:00

  • Bacterial survival in a dilute environment.

    abstract::Bacteria were isolated from lake water, and their ability to remain viable in a dilute, nutrient-deficient environment was tested by a method that permits suspension of test bacteria between two appressed microporous membranes in an aqueous environment. This approach permitted separation of the lake isolates into two ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.41.6.1331-1336.1981

    authors: Sjogren RE,Gibson MJ

    更新日期:1981-06-01 00:00:00

  • Prevalence of Escherichia coli O157 in Saskatchewan cattle: characterization of isolates by using random amplified polymorphic DNA PCR, antibiotic resistance profiles, and pathogenicity determinants.

    abstract::The prevalence of Escherichia coli O157 associated with feedlot cattle in Saskatchewan was determined in a 10-month longitudinal study (3 feedlots) and a point prevalence study (20 feedlots). The prevalence of E. coli O157 at the three different sites in the horizontal study varied from 2.5 to 45%. The point prevalenc...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02791-05

    authors: Vidovic S,Korber DR

    更新日期:2006-06-01 00:00:00

  • A Somatic Coliphage Threshold Approach To Improve the Management of Activated Sludge Wastewater Treatment Plant Effluents in Resource-Limited Regions.

    abstract::Effective wastewater management is crucial to ensure the safety of water reuse projects and effluent discharge into surface waters. Multiple studies have demonstrated that municipal wastewater treatment with conventional activated sludge processes is inefficient for the removal of a wide spectrum of viruses in sewage....

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00616-20

    authors: Chacón L,Barrantes K,Santamaría-Ulloa C,Solano M,Reyes L,Taylor L,Valiente C,Symonds EM,Achí R

    更新日期:2020-08-18 00:00:00

  • Oxidation of Nitrapyrin to 6-Chloropicolinic Acid by the Ammonia-Oxidizing Bacterium Nitrosomonas europaea.

    abstract::Suspensions of Nitrosomonas europaea catalyzed the oxidation of the commercial nitrification inhibitor nitrapyrin [2-chloro-6-(trichloromethyl)-pyridine]. Rapid oxidation of nitrapyrin (at a concentration of 10 muM) required the concomitant oxidation of ammonia, hydroxylamine, or hydrazine. The turnover rate was highe...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.58.7.2321-2325.1992

    authors: Vannelli T,Hooper AB

    更新日期:1992-07-01 00:00:00

  • Introducing SONS, a tool for operational taxonomic unit-based comparisons of microbial community memberships and structures.

    abstract::The recent advent of tools enabling statistical inferences to be drawn from comparisons of microbial communities has enabled the focus of microbial ecology to move from characterizing biodiversity to describing the distribution of that biodiversity. Although statistical tools have been developed to compare community s...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00474-06

    authors: Schloss PD,Handelsman J

    更新日期:2006-10-01 00:00:00

  • Establishment of a tractable genetic transformation system in Veillonella spp.

    abstract::We have constructed the first Escherichia coli-Veillonella shuttle vector based on an endogenous plasmid (pVJL1) isolated from a clinical Veillonella strain. A highly transformable Veillonella strain was also identified. Both the shuttle vector and the transformable strain should be valuable tools for future Veillonel...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00196-12

    authors: Liu J,Xie Z,Merritt J,Qi F

    更新日期:2012-05-01 00:00:00

  • Localizations of aluminum in soybean bacteroids and seeds.

    abstract::Aluminum, long known to be detrimental to soybean productivity, was localized in the polyphosphate granules (PPG) of bacteroids in root nodules of soybean plants. By using energy-dispersive X-ray analysis, bacteroids in early infections were shown to have typical PPG constituents. However, in PPG in older infections a...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.53.10.2548-2553.1987

    authors: Roth LE,Dunlap JR,Stacey G

    更新日期:1987-10-01 00:00:00

  • Lignin Degradation and Modification by the Soil-Inhabiting Fungus Fusarium proliferatum.

    abstract::A soil-inhabiting Fusarium proliferatum strain was capable of transforming or degrading nonlabeled and (sup14)C-labeled industrial, natural, and synthetic lignin. The mineralization rate per day (expressed as the percentage of added radioactivity recovered as to (sup14)CO(inf2)) was maximal during primary metabolism. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.63.9.3716-3718.1997

    authors: Regalado V,Rodriguez A,Perestelo F,Carnicero A,De La Fuente G,Falcon MA

    更新日期:1997-09-01 00:00:00

  • Mediation Analysis as a Means of Identifying Dietary Components That Differentially Affect the Fecal Microbiota of Infants Weaned by Modified Baby-Led and Traditional Approaches.

    abstract::The introduction of "solids" (i.e., complementary foods) to the milk-only diet in early infancy affects the development of the gut microbiota. The aim of this study was to determine whether a "baby-led" approach to complementary feeding that encourages the early introduction of an adult-type diet results in alteration...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章,随机对照试验

    doi:10.1128/AEM.00914-18

    authors: Leong C,Haszard JJ,Lawley B,Otal A,Taylor RW,Szymlek-Gay EA,Fleming EA,Daniels L,Fangupo LJ,Tannock GW,Heath AM

    更新日期:2018-08-31 00:00:00

  • Fiber-optic fluorometer for microscale mapping of photosynthetic pigments in microbial communities.

    abstract::Microscale fluorescence measurements were performed in photosynthetic biofilms at a spatial resolution of 100 to 200 microm with a new fiber-optic fluorometer which allowed four different excitation and emission wavelengths and was configured for measuring phycobiliproteins, chlorophylls, and bacteriochlorophylls. We ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.67.6.2823-2828.2001

    authors: Thar R,Kühl M,Holst G

    更新日期:2001-06-01 00:00:00

  • Identification of disulfides from the biodegradation of dibenzothiophene.

    abstract::Several investigations have identified benzothiophene-2,3-dione in the organic solvent extracts of acidified cultures degrading dibenzothiophene via the Kodama pathway. In solution at neutral pH, the 2,3-dione exists as 2-mercaptophenylglyoxylate, which cyclizes upon acidification and is extracted as the 2,3-dione. Th...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.67.11.5084-5093.2001

    authors: Bressler DC,Fedorak PM

    更新日期:2001-11-01 00:00:00

  • Metabolic pathway involved in 2-methyl-6-ethylaniline degradation by Sphingobium sp. strain MEA3-1 and cloning of the novel flavin-dependent monooxygenase system meaBA.

    abstract::2-Methyl-6-ethylaniline (MEA) is the main microbial degradation intermediate of the chloroacetanilide herbicides acetochlor and metolachlor. Sphingobium sp. strain MEA3-1 can utilize MEA and various alkyl-substituted aniline and phenol compounds as sole carbon and energy sources for growth. We isolated the mutant stra...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01883-15

    authors: Dong W,Chen Q,Hou Y,Li S,Zhuang K,Huang F,Zhou J,Li Z,Wang J,Fu L,Zhang Z,Huang Y,Wang F,Cui Z

    更新日期:2015-12-01 00:00:00

  • Glutamate decarboxylase-mediated nisin resistance in Listeria monocytogenes.

    abstract::Analysis of a complete set of glutamate decarboxylase (gad) mutants of Listeria monocytogenes strain LO28 (ΔgadD1, ΔgadDT1, ΔgadD2, ΔgadT2, and ΔgadD3 mutants) revealed that the ΔgadD1 mutant is impaired in its ability to tolerate exposure to both sublethal and lethal levels of the lantibiotic nisin. gadD1 is strain v...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00203-10

    authors: Begley M,Cotter PD,Hill C,Ross RP

    更新日期:2010-10-01 00:00:00

  • Identification and Resolution of Microdiversity through Metagenomic Sequencing of Parallel Consortia.

    abstract::To gain a predictive understanding of the interspecies interactions within microbial communities that govern community function, the genomic complement of every member population must be determined. Although metagenomic sequencing has enabled the de novo reconstruction of some microbial genomes from environmental comm...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02274-15

    authors: Nelson WC,Maezato Y,Wu YW,Romine MF,Lindemann SR

    更新日期:2015-10-23 00:00:00

  • The Sinorhizobium (Ensifer) fredii HH103 Nodulation Outer Protein NopI Is a Determinant for Efficient Nodulation of Soybean and Cowpea Plants.

    abstract::The type III secretion system (T3SS) is a specialized secretion apparatus that is commonly used by many plant and animal pathogenic bacteria to deliver proteins, termed effectors, to the interior of the host cells. These effectors suppress host defenses and interfere with signal transduction pathways to promote infect...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02770-16

    authors: Jiménez-Guerrero I,Pérez-Montaño F,Medina C,Ollero FJ,López-Baena FJ

    更新日期:2017-02-15 00:00:00

  • A PCR assay To discriminate human and ruminant feces on the basis of host differences in Bacteroides-Prevotella genes encoding 16S rRNA.

    abstract::Our purpose was to develop a rapid, inexpensive method of diagnosing the source of fecal pollution in water. In previous research, we identified Bacteroides-Prevotella ribosomal DNA (rDNA) PCR markers based on analysis. These markers length heterogeneity PCR and terminal restriction fragment length polymorphism distin...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.66.10.4571-4574.2000

    authors: Bernhard AE,Field KG

    更新日期:2000-10-01 00:00:00

  • Engineered cyanophycin synthetase (CphA) from Nostoc ellipsosporum confers enhanced CphA activity and cyanophycin accumulation to Escherichia coli.

    abstract::The cyanophycin (CGP) synthetase gene (cphANE1) of the transposon-induced argL mutant NE1 of the cyanobacterium Nostoc ellipsosporum, which exhibits a CGP-leaky phenotype during diazotrophical growth, was cloned and expressed in Escherichia coli strain TOP10. Its amino acid sequence exhibited high similarities to CphA...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01132-06

    authors: Hai T,Frey KM,Steinbüchel A

    更新日期:2006-12-01 00:00:00