Isolation of radiation-resistant bacteria without exposure to irradiation.

Abstract:

:Resistance to desiccation was utilized in the selection of highly radiation-resistant asporogenous bacteria from non-irradiated sources. A bacterial suspension in phosphate buffer was dried in a thin film at 25 degrees C and 33% relative humidity. Storage under these conditions for 15 days or more reduced the number of radiation-sensitive bacteria. Further selection for radiation-resistant bacteria was obtained by irradiation of bacteria on velveteen in the replication process, thereby avoiding the toxic effect of irradiated media. The similarity of radiation resistance and identifying characteristics in irradiated and non-irradiated isolates should allay some concerns that highly radiation-resistant bacteria have been permanently altered by radiation selection.

journal_name

Appl Environ Microbiol

authors

Sanders SW,Maxcy RB

doi

10.1128/AEM.38.3.436-439.1979

subject

Has Abstract

pub_date

1979-09-01 00:00:00

pages

436-9

issue

3

eissn

0099-2240

issn

1098-5336

journal_volume

38

pub_type

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