Associations of yeasts with spotted-wing Drosophila (Drosophila suzukii; Diptera: Drosophilidae) in cherries and raspberries.

Abstract:

:A rich history of investigation documents various Drosophila-yeast mutualisms, suggesting that Drosophila suzukii similarly has an association with a specific yeast species or community. To discover candidate yeast species, yeasts were isolated from larval frass, adult midguts, and fruit hosts of D. suzukii. Terminal restriction fragment length polymorphism (TRFLP) technology and decimal dilution plating were used to identify and determine the relative abundance of yeast species present in fruit juice samples that were either infested with D. suzukii or not infested. Yeasts were less abundant in uninfested than infested samples. A total of 126 independent yeast isolates were cultivated from frass, midguts, and fruit hosts of D. suzukii, representing 28 species of yeasts, with Hanseniaspora uvarum predominating. This suggests an association between D. suzukii and H. uvarum that could be utilized for pest management of the highly pestiferous D. suzukii.

journal_name

Appl Environ Microbiol

authors

Hamby KA,Hernández A,Boundy-Mills K,Zalom FG

doi

10.1128/AEM.00841-12

subject

Has Abstract

pub_date

2012-07-01 00:00:00

pages

4869-73

issue

14

eissn

0099-2240

issn

1098-5336

pii

AEM.00841-12

journal_volume

78

pub_type

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