Multiple Optimal Phenotypes Overcome Redox and Glycolytic Intermediate Metabolite Imbalances in Escherichia coli pgi Knockout Evolutions.

abstract：:In this study, we report the cloning and expression of lipase gene from Pseudomonas fluorescens B52, a psychrotrophic spoilage bacterium isolated from refrigerated raw milk. Sequence analysis revealed one major open reading frame of 1,428 nucleotides that was predicted to encode a protein with a molecular weight of 50...

journal_title：Applied and environmental microbiology

authors： Tan Y,Miller KJ

更新日期：1992-04-01 00:00:00

abstract：:The human immunodeficiency virus type 1 (HIV-1) released by infected individuals or present in human and hospital wastes can potentially cause contamination problems. The presence of HIV-1 was investigated in 16 environmental samples, including raw wastewater, sludge, final effluent, soil, and pond water, collected fr...

journal_title：Applied and environmental microbiology

authors： Ansari SA,Farrah SR,Chaudhry GR

更新日期：1992-12-01 00:00:00

abstract：:Short-chain fatty acid (SCFA) biosynthesis is pertinent to production of biofuels, industrial compounds, and pharmaceuticals from renewable resources. To expand on Escherichia coli SCFA products, we previously implemented a coenzyme A (CoA)-dependent pathway that condenses acetyl-CoA to a diverse group of short-chain ...

journal_title：Applied and environmental microbiology

authors： McMahon MD,Prather KL

更新日期：2014-02-01 00:00:00

abstract：:Several recent outbreaks of toxoplasmosis were related to drinking water. We propose a strategy for Toxoplasma oocyst detection as part of an approach to detecting multiple waterborne parasites, including Giardia and Cryptosporidium spp., by the U.S. Environmental Protection Agency method with the same sample. Water s...

journal_title：Applied and environmental microbiology

authors： Villena I,Aubert D,Gomis P,Ferté H,Inglard JC,Denis-Bisiaux H,Dondon JM,Pisano E,Ortis N,Pinon JM

更新日期：2004-07-01 00:00:00

abstract：:A previously identified insert expressing an endopeptidase from a Lactobacillus helveticus CNRZ32 genomic library was characterized. Nucleotide sequence analysis revealed an open reading frame of 1,941 bp encoding a putative protein of 71.2 kDa which contained a zinc-protease motif. Protein homology searches revealed ...

journal_title：Applied and environmental microbiology

authors： Chen YS,Steele JL

更新日期：1998-09-01 00:00:00

abstract：:We found that a robust energy taxis response mediated by the Aer receptor can sometimes mask chemotaxis mediated by other methyl-accepting chemotaxis proteins (MCPs) in Pseudomonas aeruginosa. We identified PA2652 as a chemoreceptor for malate by screening aer mcp double mutants by using swarm plate assays. ...

journal_title：Applied and environmental microbiology

authors： Alvarez-Ortega C,Harwood CS

更新日期：2007-12-01 00:00:00

abstract：UNLABELLED:Livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) was detected in 2003 and rapidly became the predominant MRSA clade in the Netherlands. Studies have shown that transmissions are difficult to identify, since this MRSA variant represents a genetically homogenous clade when current typ...

journal_title：Applied and environmental microbiology

authors： Bosch T,Witteveen S,Haenen A,Landman F,Schouls LM

更新日期：2016-06-30 00:00:00

abstract：:Mono Lake is an alkaline hypersaline lake that supports high methane oxidation rates. Retrieved pmoA sequences showed a broad diversity of aerobic methane oxidizers including the type I methanotrophs Methylobacter (the dominant genus), Methylomicrobium, and Methylothermus, and the type II methanotroph Methylocystis. S...

journal_title：Applied and environmental microbiology

authors： Lin JL,Joye SB,Scholten JC,Schäfer H,McDonald IR,Murrell JC

更新日期：2005-10-01 00:00:00

abstract：:Each year, thousands of new protistan 18S rRNA sequences are detected in environmental samples. Many of these sequences are molecular signatures of new protistan species, classes, and/or kingdoms that have never been seen before. The main goal of this study was to enable visualization of these novel organisms and to c...

journal_title：Applied and environmental microbiology

authors： Stoeck T,Fowle WH,Epstein SS

更新日期：2003-11-01 00:00:00

abstract：:The industrially important organism Corynebacterium glutamicum has been characterized in recent years for its robust ability to assimilate aromatic compounds. In this study, C. glutamicum strain AS 1.542 was investigated for its ability to catabolize phenylacetic acid (PAA). The paa genes were identified; they are org...

journal_title：Applied and environmental microbiology

authors： Chen X,Kohl TA,Rückert C,Rodionov DA,Li LH,Ding JY,Kalinowski J,Liu SJ

更新日期：2012-08-01 00:00:00

abstract：:By using cryo-scanning electron microscopy and quantification with lectin-conjugated probes, we have detected the production of exopolysaccharides (EPS) in Bifidobacterium animalis subsp. lactis in the presence of bile. In addition, the expression of gtf01207, which codifies a putative priming glycosyltransferase invo...

journal_title：Applied and environmental microbiology

authors： Ruas-Madiedo P,Gueimonde M,Arigoni F,de los Reyes-Gavilán CG,Margolles A

更新日期：2009-02-01 00:00:00

abstract：:Communities of ammonia-oxidizing archaea (AOA) and bacteria (AOB) in freshwater sediments and those in association with the root system of the macrophyte species Littorella uniflora, Juncus bulbosus, and Myriophyllum alterniflorum were compared for seven oligotrophic to mesotrophic softwater lakes and acidic heathland...

journal_title：Applied and environmental microbiology

authors： Herrmann M,Saunders AM,Schramm A

更新日期：2009-05-01 00:00:00

abstract：:We report the spontaneous formation of a stable mannitol-producing variant of Leuconostoc pseudomesenteroides. The mannitol-producing variant showed mannitol dehydrogenase activity which was absent in the parental strain. It was also able to use fructose and glucose simultaneously, whereas the parental strain showed d...

journal_title：Applied and environmental microbiology

authors： Grobben GJ,Peters SW,Wisselink HW,Weusthuis RA,Hoefnagel MH,Hugenholtz J,Eggink G

更新日期：2001-06-01 00:00:00

abstract：:A new design, flat-bottom combination pH electrode was evaluated for utility in determining the pH of prepared agar media by surface contact of the electrode with the agar media. ...

journal_title：Applied and environmental microbiology

authors： Gorski TW,Ritzert RW

更新日期：1977-08-01 00:00:00

abstract：:To understand phage infection and host cell lysis mechanisms in pathogenic Salmonella, a novel Salmonella enterica serovar Typhimurium-targeting bacteriophage, SPN9CC, belonging to the Podoviridae family was isolated and characterized. The phage infects S. Typhimurium via the O antigen of lipopolysaccharide (LPS) and ...

journal_title：Applied and environmental microbiology

authors： Shin H,Lee JH,Yoon H,Kang DH,Ryu S

更新日期：2014-01-01 00:00:00

abstract：:Under anoxic conditions, most methoxylated mononuclear aromatic compounds are degraded by bacteria, with catechol being formed as an important intermediate. On the basis of our experiments with the sulfate-reducing bacterium Desulfobacterium sp. strain Cat2, we describe for the first time the enzymatic activities invo...

journal_title：Applied and environmental microbiology

authors： Gorny N,Schink B

更新日期：1994-09-01 00:00:00

abstract：:Quaternary ammonium compounds such as benzalkonium chloride (BC) are widely used as disinfectants in both food processing and medical environments. BC-resistant strains of Listeria monocytogenes have been implicated in multistate outbreaks of listeriosis and have been frequently isolated from food processing plants. H...

journal_title：Applied and environmental microbiology

authors： Elhanafi D,Dutta V,Kathariou S

更新日期：2010-12-01 00:00:00

abstract：:Mongolian gerbils were used as an animal model to excyst and host Giardia spp. isolated from meadow voles, dogs, beavers, and humans. Both cysts and trophozoites were used to establish infections. Gerbils were infected with Giardia duodenalis from beaver, dog, and human sources, and the trophozoites were extracted and...

journal_title：Applied and environmental microbiology

authors： Wallis PM,Wallis HM

更新日期：1986-03-01 00:00:00

abstract：:We report the construction of an Escherichia coli mutant that harbors two compatible plasmids and that is able to synthesize labeled 2-O-alpha-D-mannosyl-D-glycerate from externally added labeled mannose without the loss of specific isotopic enrichment. The strain carries a deletion in the manA gene, encoding phosphom...

journal_title：Applied and environmental microbiology

authors： Sampaio MM,Santos H,Boos W

更新日期：2003-01-01 00:00:00

abstract：:The effect of autoproteolysis of Lactococcus lactis lactocepin III on its specificity towards beta-casein was investigated. beta-Casein degradation was performed by using either an autolysin-defective derivative of L. lactis MG1363 carrying the proteinase genes of L. lactis SK11, which was unable to transport oligopep...

journal_title：Applied and environmental microbiology

authors： Flambard B,Juillard V

更新日期：2000-12-01 00:00:00

abstract：:Very little is known about the microbial composition of animal bedding wastes, including poultry litter, and what is known has been deduced from standard culture methods, by which some fastidious organisms that exist in the environment may not be detected. We evaluated the bacterial composition of poultry litter by us...

journal_title：Applied and environmental microbiology

authors： Lu J,Sanchez S,Hofacre C,Maurer JJ,Harmon BG,Lee MD

更新日期：2003-02-01 00:00:00

abstract：:Genetic manipulation of the category B select agents Burkholderia pseudomallei and Burkholderia mallei has been stifled due to the lack of compliant selectable markers. Hence, there is a need for additional select-agent-compliant selectable markers. We engineered a selectable marker based on the gat gene (encoding gly...

journal_title：Applied and environmental microbiology

authors： Norris MH,Kang Y,Lu D,Wilcox BA,Hoang TT

更新日期：2009-10-01 00:00:00

abstract：:The mechanisms of metal resistance of a cadmium-resistant Alcaligenes sp. were studied. Growth in a defined medium was unaffected by cadmium at concentrations up to 0.1 mM, while at concentrations up to 2.5 mM, growth occurred after an extended lag phase. The increase in length of the lag phase was abolished by repeat...

journal_title：Applied and environmental microbiology

authors： McEntee JD,Woodrow JR,Quirk AV

更新日期：1986-03-01 00:00:00

abstract：:A microscopically pure enrichment culture of a gram-negative anaerobic bacterium, in the present article referred to as PER-K23, was isolated from an anaerobic packed-bed column in which tetrachloroethene (PCE) was reductively transformed to ethane via trichloroethene (TCE), cis-1,2-dichloroethene (cis-1,2-DCE), chlor...

journal_title：Applied and environmental microbiology

authors： Holliger C,Schraa G,Stams AJ,Zehnder AJ

更新日期：1993-09-01 00:00:00

abstract：:Microbial transformation of lapachol, a naturally occurring naphthoquinone, was carried out by Curvularia lunata (NRRL 2178). The fungus brings about oxidative cyclization of the substrate to dehydro-alpha-lapachone, which was isolated and characterized by nuclear magnetic resonance and mass spectral analyses; its str...

journal_title：Applied and environmental microbiology

authors： Otten S,Rosazza JP

更新日期：1979-08-01 00:00:00

abstract：:Deletion of the pcaHG genes, encoding protocatechuate 3,4-dioxygenase in Rhodococcus jostii RHA1, gives a gene deletion strain still able to grow on protocatechuic acid as the sole carbon source, indicating a second degradation pathway for protocatechuic acid. Metabolite analysis of wild-type R. jostii RHA1 grown on m...

journal_title：Applied and environmental microbiology

authors： Spence EM,Scott HT,Dumond L,Calvo-Bado L,di Monaco S,Williamson JJ,Persinoti GF,Squina FM,Bugg TDH

更新日期：2020-09-17 00:00:00

abstract：:Campylobacter jejuni and Campylobacter coli are naturally competent, but limited information exists on the impact of environmental conditions on transformation. In this study, we investigated the impact of temperature and microaerobic versus aerobic atmosphere on transformation of C. coli to erythromycin and nalidixic...

journal_title：Applied and environmental microbiology

authors： Kim JS,Kim JW,Kathariou S

更新日期：2008-10-01 00:00:00

abstract：:Twenty Gateway-compatible destination vectors were constructed. The vectors comprise fluorescent and epitope fusion tags, various drug markers, and replication origins that should make them useful for exploring existing microbial ORFeomes. In an attempt to validate several of these vectors, we observed polar and oscil...

journal_title：Applied and environmental microbiology

authors： Hallez R,Letesson JJ,Vandenhaute J,De Bolle X

更新日期：2007-02-01 00:00:00

abstract：:Three species of saltmarsh ascomycetes were grown in the presence of all of the constituents of their natural substrate (leaves of cordgrass) or were presented only with aqueous extracts of the leaves. These two growth-condition treatments had no significant effect on total ergosterol content of the fungal mycelia, co...

journal_title：Applied and environmental microbiology

authors： Newell SY

更新日期：1994-09-01 00:00:00

abstract：:Artificially designed gelatins comprising tandemly repeated 30-amino-acid peptide units derived from human alphaI collagen were successfully produced with a Bacillus brevis system. The DNA encoding the peptide unit was synthesized by taking into consideration the codon usage of the host cells, but no clones having a t...

journal_title：Applied and environmental microbiology

authors： Kajino T,Takahashi H,Hirai M,Yamada Y

更新日期：2000-01-01 00:00:00