Cloning, expression, and nucleotide sequence of a lipase gene from Pseudomonas fluorescens B52.

Abstract:

:In this study, we report the cloning and expression of lipase gene from Pseudomonas fluorescens B52, a psychrotrophic spoilage bacterium isolated from refrigerated raw milk. Sequence analysis revealed one major open reading frame of 1,428 nucleotides that was predicted to encode a protein with a molecular weight of 50,241. The predicted enzyme was found to contain an amino acid sequence highly homologous to the putative substrate-binding domain present within all lipases examined to date.

journal_name

Appl Environ Microbiol

authors

Tan Y,Miller KJ

doi

10.1128/AEM.58.4.1402-1407.1992

keywords:

subject

Has Abstract

pub_date

1992-04-01 00:00:00

pages

1402-7

issue

4

eissn

0099-2240

issn

1098-5336

journal_volume

58

pub_type

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