Microbial transformations of natural antitumor agents: conversion of lapachol to dehydro-alpha-lapachone by Curvularia lunata.

abstract：:The current project was developed to examine inactivation of biofilm bacteria and to characterize the interaction of biocides with pipe surfaces. Unattached bacteria were quite susceptible to the variety of disinfectants tested. Viable bacterial counts were reduced 99% by exposure to 0.08 mg of hypochlorous acid (pH 7...

journal_title：Applied and environmental microbiology

authors： LeChevallier MW,Cawthon CD,Lee RG

更新日期：1988-10-01 00:00:00

abstract：:The effect of autoproteolysis of Lactococcus lactis lactocepin III on its specificity towards beta-casein was investigated. beta-Casein degradation was performed by using either an autolysin-defective derivative of L. lactis MG1363 carrying the proteinase genes of L. lactis SK11, which was unable to transport oligopep...

journal_title：Applied and environmental microbiology

authors： Flambard B,Juillard V

更新日期：2000-12-01 00:00:00

abstract：:Opaque and translucent morphotypes of a TnphoA-containing strain of Vibrio vulnificus were fed to oysters, which were subsequently stored at temperatures ranging from 0.5 to 22 degrees C for 10 days. Samples of oysters were homogenized and plated at intervals to determine the cell density of V. vulnificus and total ae...

journal_title：Applied and environmental microbiology

authors： Murphy SK,Oliver JD

更新日期：1992-09-01 00:00:00

abstract：:Allozyme electrophoresis and restriction fragment length polymorphism (RFLP) analyses were used to examine the genetic diversity of a collection of 18 Rhizobium leguminosarum bv. trifolii, 1 R. leguminosarum bv. viciae, and 2 R. meliloti strains. Allozyme analysis at 28 loci revealed 16 electrophoretic types. The mean...

journal_title：Applied and environmental microbiology

authors： Demezas DH,Reardon TB,Watson JM,Gibson AH

更新日期：1991-12-01 00:00:00

abstract：:Human beta-hexosaminidase A (HexA) is a heterodimeric glycoprotein composed of alpha- and beta-subunits that degrades GM2 gangliosides in lysosomes. GM2 gangliosidosis is a lysosomal storage disease in which an inherited deficiency of HexA causes the accumulation of GM2 gangliosides. In order to prepare a large amount...

journal_title：Applied and environmental microbiology

authors： Akeboshi H,Chiba Y,Kasahara Y,Takashiba M,Takaoka Y,Ohsawa M,Tajima Y,Kawashima I,Tsuji D,Itoh K,Sakuraba H,Jigami Y

更新日期：2007-08-01 00:00:00

abstract：:Cyanase catalyzes the decomposition of cyanate into CO(2) and ammonium, with carbamate as an unstable intermediate. The cyanase of Pseudomonas pseudoalcaligenes CECT5344 was negatively regulated by ammonium and positively regulated by cyanate, cyanide, and some cyanometallic complexes. Cyanase activity was not detecte...

journal_title：Applied and environmental microbiology

authors： Luque-Almagro VM,Huertas MJ,Sáez LP,Luque-Romero MM,Moreno-Vivián C,Castillo F,Roldán MD,Blasco R

更新日期：2008-10-01 00:00:00

abstract：:Four soil temperature and moisture treatment regimens were imposed on Florunner peanuts 94 days after planting in experimental plots in 1980. At harvest (145 days after planting), the incidence of the Aspergillus flavus group and the aflatoxin concentration were greatest in damaged kernels. Extensive colonization of s...

journal_title：Applied and environmental microbiology

authors： Hill RA,Blankenship PD,Cole RJ,Sanders TH

更新日期：1983-02-01 00:00:00

abstract：:Anaerobic degradation of polycyclic aromatic hydrocarbons has been investigated mostly with naphthalene as a model compound. Naphthalene degradation by sulfate-reducing bacteria proceeds via carboxylation to 2-naphthoic acid, formation of a coenzyme A thioester, and subsequent reduction to 5,6,7,8-tetrahydro-2-naphtho...

journal_title：Applied and environmental microbiology

authors： Weyrauch P,Heker I,Zaytsev AV,von Hagen CA,Arnold ME,Golding BT,Meckenstock RU

更新日期：2020-07-20 00:00:00

abstract：:The active population of low-affinity methanotrophs in a peat soil microcosm was characterized by stable-isotope probing. "Heavy" (13)C-labeled DNA, produced after microbial growth on (13)CH(4), was separated from naturally abundant (12)C-DNA by cesium chloride density gradient centrifugation and used as a template fo...

journal_title：Applied and environmental microbiology

authors： Morris SA,Radajewski S,Willison TW,Murrell JC

更新日期：2002-03-01 00:00:00

abstract：:A point mutation (E115K) resulting in slower growth of Escherichia coli DH5alpha and XL1-Blue in minimal media was identified in the purB gene, coding for adenylosuccinate lyase (ASL), through complementation with an E. coli K-12 genomic library and serial subcultures. Chromosomal modification reversing the mutation t...

journal_title：Applied and environmental microbiology

authors： Jung SC,Smith CL,Lee KS,Hong ME,Kweon DH,Stephanopoulos G,Jin YS

更新日期：2010-09-01 00:00:00

abstract：:Two genetically engineered microorganisms (GEMs), Pseudomonas sp. strain B13 FR1(pFRC20P) (FR120) and Pseudomonas putida KT2440(pWWO-EB62) (EB62), were introduced into activated sludge microcosms that had the level of aeration, nutrient makeup, and microbial community structure of activated sludge reactors. FR120 cont...

journal_title：Applied and environmental microbiology

authors： Nüsslein K,Maris D,Timmis K,Dwyer DF

更新日期：1992-10-01 00:00:00

abstract：:Microbacterium thermosphactum requires cysteine, alpha-lipoate, nicotinate, pantothenate, p-aminobenzoate, biotin, and thiamin for aerobic growth in glucose-mineral salts medium. Glucose cannot be replaced by Casamino Acids or acids of the tricarboxylic acid cycle as sole carbon and energy sources. The organism can al...

journal_title：Applied and environmental microbiology

authors： Grau FH

更新日期：1979-11-01 00:00:00

abstract：:Several recent outbreaks of toxoplasmosis were related to drinking water. We propose a strategy for Toxoplasma oocyst detection as part of an approach to detecting multiple waterborne parasites, including Giardia and Cryptosporidium spp., by the U.S. Environmental Protection Agency method with the same sample. Water s...

journal_title：Applied and environmental microbiology

authors： Villena I,Aubert D,Gomis P,Ferté H,Inglard JC,Denis-Bisiaux H,Dondon JM,Pisano E,Ortis N,Pinon JM

更新日期：2004-07-01 00:00:00

abstract：:The spatiotemporal distributions of Aeromonas spp. and fecal coliforms in a cove receiving sewage treatment effluent and draining into a brackish lagoon were studied for 34 months with sampling at six stations. A total of 452 strains of Aeromonas spp. were isolated and identified at the outflow of the treatment system...

journal_title：Applied and environmental microbiology

authors： Monfort P,Baleux B

更新日期：1991-09-01 00:00:00

abstract：:Transpositional mutagenesis of Thiobacillus novellus by Tn501 was achieved by means of the incompatibility of IncP plasmids. Tn501 insertion caused three types of mutant phenotypes: isoleucine auxotrophy, lysine auxotrophy, and a reduced ability to oxidize reduced sulfur compounds and to fix CO(2). Oxidation rates for...

journal_title：Applied and environmental microbiology

authors： Davidson MS,Roy P,Summers AO

更新日期：1985-06-01 00:00:00

abstract：:Clostridium sp. strain S1, an unnamed bile acid-desulfating strain from rat intestinal microflora (S.M. Huijghebaert, J. A. Mertens, and H. J. Eyssen, Appl. Environ. Microbiol. 43:185-192, 1982), was examined for its ability to desulfate different bile acid sulfates and steroid sulfates in growing cultures. Clostridiu...

journal_title：Applied and environmental microbiology

authors： Huijghebaert SM,Eyssen HJ

更新日期：1982-11-01 00:00:00

abstract：:Procedures based on DNA hybridization and PCR were developed for quality control of Rhizobium inoculants. Inoculants for pea and goat's rue were produced by Elomestari Ltd., Juva, Finland, in sterile dry fine peat by the standard procedure used by the company. The inoculants contained Rhizobium galegae HAMBI 1174 and ...

journal_title：Applied and environmental microbiology

authors： Tas E,Saano A,Leinonen P,Lindström K

更新日期：1995-05-01 00:00:00

abstract：:Estimations of the bacterial content of air can be more easily made now than a decade ago, with colony formation the method of choice for enumeration of airborne bacteria. However, plate counts are subject to error because bacteria exposed to the air may remain viable yet lose the ability to form colonies, i.e., they ...

journal_title：Applied and environmental microbiology

authors： Heidelberg JF,Shahamat M,Levin M,Rahman I,Stelma G,Grim C,Colwell RR

更新日期：1997-09-01 00:00:00

abstract：:Carbazole 1,9a-dioxygenase (CARDO), a Rieske nonheme iron oxygenase (RO), is a three-component system composed of a terminal oxygenase (Oxy), ferredoxin, and a ferredoxin reductase. Oxy has angular dioxygenation activity against carbazole. Previously, site-directed mutagenesis of the Oxy-encoding gene from Janthinobac...

journal_title：Applied and environmental microbiology

authors： Inoue K,Usami Y,Ashikawa Y,Noguchi H,Umeda T,Yamagami-Ashikawa A,Horisaki T,Uchimura H,Terada T,Nakamura S,Shimizu K,Habe H,Yamane H,Fujimoto Z,Nojiri H

更新日期：2014-05-01 00:00:00

abstract：:Time series incubations were conducted to provide estimates for the size selectivities and rates of protistan grazing that may be occurring in a sandy, contaminated aquifer. The experiments involved four size classes of fluorescently labeled groundwater bacteria (FLB) and 2- to 3-microns-long nanoflagellates, primaril...

journal_title：Applied and environmental microbiology

authors： Kinner NE,Harvey RW,Blakeslee K,Novarino G,Meeker LD

更新日期：1998-02-01 00:00:00

abstract：:The use of the phospholipid fatty acid (PLFA) composition of microorganisms to detect previous self-heating events was studied in naturally self-heated peat and in peat incubated under temperature-controlled conditions. An increased content of total PLFAs was found in self-heated peat compared to that in unheated peat...

journal_title：Applied and environmental microbiology

authors： Ranneklev SB,Bååth E

更新日期：2003-06-01 00:00:00

abstract：:Since Vibrio cholerae O139 first appeared in 1992, both O1 El Tor and O139 have been recognized as the epidemic serogroups, although their geographic distribution, endemicity, and reservoir are not fully understood. To address this lack of information, a study of the epidemiology and ecology of V. cholerae O1 and O139...

journal_title：Applied and environmental microbiology

authors： Alam M,Hasan NA,Sadique A,Bhuiyan NA,Ahmed KU,Nusrin S,Nair GB,Siddique AK,Sack RB,Sack DA,Huq A,Colwell RR

更新日期：2006-06-01 00:00:00

abstract：:The transmission of Escherichia coli O157:H7 from manure-contaminated soil and irrigation water to lettuce plants was demonstrated using laser scanning confocal microscopy, epifluorescence microscopy, and recovery of viable cells from the inner tissues of plants. E. coli O157:H7 migrated to internal locations in plant...

journal_title：Applied and environmental microbiology

authors： Solomon EB,Yaron S,Matthews KR

更新日期：2002-01-01 00:00:00

abstract：:A defined solid and liquid minimal medium, HTM, which contained methionine and cysteine as the sole amino acids, was developed for Listeria monocytogenes. Complex broth-grown L. monocytogenes had to adapt to HTM by inducing amino acid biosyntheis. HTM is the simplest minimal medium available for growth of L. monocytog...

journal_title：Applied and environmental microbiology

authors： Tsai HN,Hodgson DA

更新日期：2003-11-01 00:00:00

abstract：:The presence of human-pathogenic, enteric bacteria on the surface and in the interior of raw produce is a significant health concern. Several aspects of the biology of the interaction between these bacteria and alfalfa (Medicago sativa) seedlings are addressed here. A collection of enteric bacteria associated with alf...

journal_title：Applied and environmental microbiology

authors： Dong Y,Iniguez AL,Ahmer BM,Triplett EW

更新日期：2003-03-01 00:00:00

abstract：:Injury induced in Escherichia coli cells by chlorination was studied from a physiological standpoint. Predictable and reproducible injury was found to occur rapidly in 0.5 mg of chlorine per liter and was reversible under nonselective conditions. There was an extended lag period in the growth of chlorinated cells not ...

journal_title：Applied and environmental microbiology

authors： Camper AK,McFeters GA

更新日期：1979-03-01 00:00:00

abstract：:Forty-two Helicobacter isolates were isolated from swine feces in The Netherlands and Denmark. All 12 isolates sequenced (16S rRNA gene) formed a robust clade with Helicobacter canadensis ( approximately 99% similarity). Species-specific PCR indicated that all of the isolates were H. canadensis isolates. Although the ...

journal_title：Applied and environmental microbiology

authors： Inglis GD,McConville M,de Jong A

更新日期：2006-06-01 00:00:00

abstract：:During growth on xylan and xylose Thermoanaerobacterium saccharolyticum B6A-RI produced endoxylanase, beta-xylosidase, arabinofuranosidase, and acetyl esterase, and the first three activities appeared to be produced coordinately. During nonlimiting growth on xylan, these enzyme activities were predominantly cell assoc...

journal_title：Applied and environmental microbiology

authors： Lee YE,Lowe SE,Zeikus JG

更新日期：1993-03-01 00:00:00

abstract：:Trehalose, a disaccharide accumulated by many microorganisms, acts as a protectant during periods of physiological stress, such as salinity and desiccation. Previous studies reported that the trehalose biosynthetic genes (otsA, treS, and treY) in Bradyrhizobium japonicum were induced by salinity and desiccation stress...

journal_title：Applied and environmental microbiology

authors： Sugawara M,Cytryn EJ,Sadowsky MJ

更新日期：2010-02-01 00:00:00

abstract：:Microbial mercury methylation and methylmercury decomposition were examined in Lake Clara, an oligotrophic northern Wisconsin seepage lake, using radioisotopic tracers. Methylation activity was near background in the water column, was greatest in the profundal surficial sediments, and decreased with depth in sediment ...

journal_title：Applied and environmental microbiology

authors： Korthals ET,Winfrey MR

更新日期：1987-10-01 00:00:00