Regulation and Characterization of Xylanolytic Enzymes of Thermoanaerobacterium saccharolyticum B6A-RI.

Abstract:

:During growth on xylan and xylose Thermoanaerobacterium saccharolyticum B6A-RI produced endoxylanase, beta-xylosidase, arabinofuranosidase, and acetyl esterase, and the first three activities appeared to be produced coordinately. During nonlimiting growth on xylan, these enzyme activities were predominantly cell associated; however, during growth on limiting concentrations of xylan, the majority of endoxylanase activity was extracellular rather than cell associated. Endoxylanase, beta-xylosidase, and arabinofuranosidase activities were induced by xylan, xylose, and arabinose, respectively. Acetyl esterase activity was constitutive, and endoxylanase activity was catabolite repressed by glucose. Extracellular endoxylanase existed as a high-molecular-weight complex (molecular weight, more than 10). When analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and zymograms, the crude endoxylanase complex was composed of at least six activity bands. Endoxylanase was purified by gel filtration with Sephacryl S-300 and affinity chromatography with xylan coupled to Sepharose CL-4B preequilibrated to 45 degrees C with 50 mM sodium acetate buffer (pH 4.0) and eluted with 0.1% soluble xylan. A single area of endoxylanase activity was identified on the zymogram; when this activity was analyzed by SDS-PAGE, it was composed of a major protein with a molecular weight of approximately 160,000 and a minor protein with a molecular weight of approximately 130,000. The endoxylanase activity stained with Schiff's reagent, indicative of glycoproteins, displayed a specific activity of 41 U/mg of protein on xylan, and had pH and temperature optima of 6.0 and 70 degrees C, respectively.

journal_name

Appl Environ Microbiol

authors

Lee YE,Lowe SE,Zeikus JG

doi

10.1128/AEM.59.3.763-771.1993

keywords:

subject

Has Abstract

pub_date

1993-03-01 00:00:00

pages

763-71

issue

3

eissn

0099-2240

issn

1098-5336

journal_volume

59

pub_type

杂志文章
  • Use of Polymerase Chain Reaction To Detect the Take-All Fungus, Gaeumannomyces graminis, in Infected Wheat Plants.

    abstract::Gaeumannomyces graminis, the causative agent of take-all disease of wheat, barley, and oats, was detected in infected wheat seedlings by using the polymerase chain reaction to amplify Gaeumannomyces-specific DNA fragments. Nested primers and two rounds of amplification were used to amplify two fragments, approximately...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.57.2.553-556.1991

    authors: Schesser K,Luder A,Henson JM

    更新日期:1991-02-01 00:00:00

  • Intracellular accumulation of polyphosphate by the yeast Candida humicola G-1 in response to acid pH.

    abstract::Cells of a newly isolated environmental strain of Candida humicola accumulated 10-fold more polyphosphate (polyP), during active growth, when grown in complete glucose-mineral salts medium at pH 5.5 than when grown at pH 7.5. Neither phosphate starvation, nutrient limitation, nor anaerobiosis was required to induce po...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.66.9.4068-4073.2000

    authors: McGrath JW,Quinn JP

    更新日期:2000-09-01 00:00:00

  • Evidence for interspecies gene transfer in the evolution of 2,4-dichlorophenoxyacetic acid degraders.

    abstract::Small-subunit ribosomal DNA (SSU rDNA) from 20 phenotypically distinct strains of 2,4-dichlorophenoxyacetic acid (2,4-D)-degrading bacteria was partially sequenced, yielding 18 unique strains belonging to members of the alpha, beta, and gamma subgroups of the class Proteobacteria. To understand the origin of 2,4-D deg...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.64.10.4089-4092.1998

    authors: McGowan C,Fulthorpe R,Wright A,Tiedje JM

    更新日期:1998-10-01 00:00:00

  • Inhibition of plant-pathogenic bacteria by short synthetic cecropin A-melittin hybrid peptides.

    abstract::Short peptides of 11 residues were synthesized and tested against the economically important plant pathogenic bacteria Erwinia amylovora, Pseudomonas syringae, and Xanthomonas vesicatoria and compared to the previously described peptide Pep3 (WKLFKKILKVL-NH(2)). The antimicrobial activity of Pep3 and 22 analogues was ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.72.5.3302-3308.2006

    authors: Ferre R,Badosa E,Feliu L,Planas M,Montesinos E,Bardají E

    更新日期:2006-05-01 00:00:00

  • Extracellular protease of Pseudomonas fluorescens CHA0, a biocontrol factor with activity against the root-knot nematode Meloidogyne incognita.

    abstract::In Pseudomonas fluorescens CHA0, mutation of the GacA-controlled aprA gene (encoding the major extracellular protease) or the gacA regulatory gene resulted in reduced biocontrol activity against the root-knot nematode Meloidogyne incognita during tomato and soybean infection. Culture supernatants of strain CHA0 inhibi...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.71.9.5646-5649.2005

    authors: Siddiqui IA,Haas D,Heeb S

    更新日期:2005-09-01 00:00:00

  • Rapid detection of meat spoilage by measuring volatile organic compounds by using proton transfer reaction mass spectrometry.

    abstract::The evolution of the microbial spoilage population for air- and vacuum-packaged meat (beef and pork) stored at 4 degrees C was investigated over 11 days. We monitored the viable counts (mesophilic total aerobic bacteria, Pseudomonas spp., Enterobacteriaceae, lactic acid bacteria, and Enterococcus spp.) by the microbio...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.69.8.4697-4705.2003

    authors: Mayr D,Margesin R,Klingsbichel E,Hartungen E,Jenewein D,Schinner F,Märk TD

    更新日期:2003-08-01 00:00:00

  • Divergent Co-occurrence Patterns and Assembly Processes Structure the Abundant and Rare Bacterial Communities in a Salt Marsh Ecosystem.

    abstract::Understanding how species interaction and assembly processes structure the abundant and rare bacterial biospheres in soils is crucial for predicting how biodiversity influences ecosystem functioning. Here, we profiled the bacterial communities across a salt marsh ecosystem gradient to investigate the co-occurrence pat...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00322-20

    authors: Du S,Dini-Andreote F,Zhang N,Liang C,Yao Z,Zhang H,Zhang D

    更新日期:2020-06-17 00:00:00

  • Stable isotopic studies of n-alkane metabolism by a sulfate-reducing bacterial enrichment culture.

    abstract::Gas chromatography-mass spectrometry and nuclear magnetic resonance spectroscopy were used to study the metabolism of deuterated n-alkanes (C6 to C12) and 1-13C-labeled n-hexane by a highly enriched sulfate-reducing bacterial culture. All substrates were activated via fumarate addition to form the corresponding alkyls...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.71.12.8174-8182.2005

    authors: Davidova IA,Gieg LM,Nanny M,Kropp KG,Suflita JM

    更新日期:2005-12-01 00:00:00

  • Sulfur isotope enrichment during maintenance metabolism in the thermophilic sulfate-reducing bacterium Desulfotomaculum putei.

    abstract::Values of Delta(34)S (=delta(34)S(HS)-delta(34)S(SO(4)), where delta(34)S(HS) and delta(34)S(SO(4)) indicate the differences in the isotopic compositions of the HS(-) and SO(4)(2-) in the eluent, respectively) for many modern marine sediments are in the range of -55 to -75 per thousand, much greater than the -2 to -46...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02948-08

    authors: Davidson MM,Bisher ME,Pratt LM,Fong J,Southam G,Pfiffner SM,Reches Z,Onstott TC

    更新日期:2009-09-01 00:00:00

  • Ethylene Production by Pseudomonas syringae Pathovars In Vitro and In Planta.

    abstract::Significant amounts of ethylene were produced by Pseudomonas syringae pv. glycinea, pv. phaseolicola (which had been isolated from viny weed Pueraria lobata [Willd.] Ohwi [common name, kudzu]), and pv. pisi in synthetic medium. On the other hand, the bean strains of P. syringae pv. phaseolicola and strains of 17 other...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.63.1.156-161.1997

    authors: Weingart H,Volksch B

    更新日期:1997-01-01 00:00:00

  • Insecticidal Activity of the Toxins from Bacillus thuringiensis subspecies kurstaki and tenebrionis Adsorbed and Bound on Pure and Soil Clays.

    abstract::The release of transgenic plants and microorganisms expressing truncated genes from various subspecies of Bacillus thuringiensis that encode active insecticidal toxins rather than inactive protoxins could result in the accumulation of these active proteins in soil, especially when bound on clays and other soil particl...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.61.5.1786-1790.1995

    authors: Tapp H,Stotzky G

    更新日期:1995-05-01 00:00:00

  • Ribulose-1,5-bisphosphate carboxylase/oxygenase gene expression and diversity of Lake Erie planktonic microorganisms.

    abstract::Carbon dioxide fixation is carried out primarily through the Calvin-Benson-Bassham reductive pentose phosphate cycle, in which ribulose-1, 5-bisphosphate carboxylase/oxygenase (RubisCO) is the key enzyme. The primary structure of the large subunit of form I RubisCO is well conserved; however, four distinct types, A, B...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.62.6.1913-1921.1996

    authors: Xu HH,Tabita FR

    更新日期:1996-06-01 00:00:00

  • Phylogenetic analysis and in situ identification of bacteria community composition in an acidic Sphagnum peat bog.

    abstract::The Bacteria community composition in an acidic Sphagnum peat bog (pH 3.9 to 4.5) was characterized by a combination of 16S rRNA gene clone library analysis, rRNA-targeted fluorescence in situ hybridization (FISH), and cultivation. Among 84 environmental 16S rRNA gene clones, a set of only 16 cloned sequences was clos...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.72.3.2110-2117.2006

    authors: Dedysh SN,Pankratov TA,Belova SE,Kulichevskaya IS,Liesack W

    更新日期:2006-03-01 00:00:00

  • Shigella sonnei Does Not Use Amoebae as Protective Hosts.

    abstract::Shigella flexneri and Shigella sonnei bacteria cause the majority of all shigellosis cases worldwide. However, their distributions differ, with S. sonnei predominating in middle- and high-income countries and S. flexneri predominating in low-income countries. One proposed explanation for the continued range expansion ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02679-17

    authors: Watson J,Jenkins C,Clements A

    更新日期:2018-04-16 00:00:00

  • Dynamics of introduced populations of Phragmidium violaceum and implications for biological control of European blackberry in Australia.

    abstract::Phragmidium violaceum causes leaf rust on the European blackberry (Rubus fruticosus L. aggregate). Multiple strains of this pathogen have been introduced into southern Australia for the biological control of at least 15 taxa of European blackberry, a nonindigenous, invasive plant. In climates conducive to leaf rust, t...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02885-07

    authors: Gomez DR,Evans KJ,Baker J,Harvey PR,Scott ES

    更新日期:2008-09-01 00:00:00

  • Automated systems for identification of heterotrophic marine bacteria on the basis of their Fatty Acid composition.

    abstract::The fatty acid methyl ester composition of a total of 71 marine strains representing the genera Alteromonas, Deleya, Oceanospirillum, and Vibrio was determined by gas-liquid chromatographic analysis. Over 70 different fatty acids were found. The predominant fatty acids were 16:0, 16:1 cis 9, summed-in-feature (SIF) 4 ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.62.6.2122-2132.1996

    authors: Bertone S,Giacomini M,Ruggiero C,Piccarolo C,Calegari L

    更新日期:1996-06-01 00:00:00

  • Demethylation of Veratrole by Cytochrome P-450 in Streptomyces setonii.

    abstract::The actinomycete Streptomyces setonii 75Vi2 demethylates vanillic acid and guaiacol to protocatechuic acid and catechol, respectively, and then metabolizes the products by the beta-ketoadipate pathway. UV spectroscopy showed that this strain could also metabolize veratrole (1,2-dimethoxybenzene). When grown in veratro...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.52.1.98-100.1986

    authors: Sutherland JB

    更新日期:1986-07-01 00:00:00

  • Circular Permutation of the Genome of a Temperate Bacteriophage from Streptococcus cremoris BK5.

    abstract::The temperate bacteriophage BK5-T was isolated from Streptococcus cremoris BK5 by induction with mitomycin C. Electron microscopy revealed that BK5-T DNA consists of linear molecules, ranging in size from 39.7 to 46 kilobase pairs. Restriction analysis of self-ligated BK5-T DNA showed that the ends of the DNA were not...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.54.4.1039-1045.1988

    authors: Lakshmidevi G,Davidson BE,Hillier AJ

    更新日期:1988-04-01 00:00:00

  • Identification of the gene encoding the major cellobiohydrolase of the white rot fungus Phanerochaete chrysosporium.

    abstract::Previous studies have shown that the cellobiohydrolases of the white rot basidiomycete Phanerochaete chrysosporium are encoded by a family of structurally related genes. In this investigation, we identified and sequenced the most highly transcribed gene, cbh1-4. Evidence suggests that in this fungus the dominant isozy...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.59.10.3492-3494.1993

    authors: Vanden Wymelenberg A,Covert S,Cullen D

    更新日期:1993-10-01 00:00:00

  • Diversity of Salmonella strains isolated from the aquatic environment as determined by serotyping and amplification of the ribosomal DNA spacer regions.

    abstract::Salmonella species are pathogenic bacteria often detected in sewage, freshwater, marine coastal water, and groundwater. Salmonella spp. can survive for long periods in natural waters, and the persistence of specific and epidemic strains is of great concern in public health. However, the diversity of species found in t...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.66.4.1544-1552.2000

    authors: Baudart J,Lemarchand K,Brisabois A,Lebaron P

    更新日期:2000-04-01 00:00:00

  • Production of glutaconic acid in a recombinant Escherichia coli strain.

    abstract::The assembly of six genes that encode enzymes from glutamate-fermenting bacteria converted Escherichia coli into a glutaconate producer when grown anaerobically on a complex medium. The new anaerobic pathway starts with 2-oxoglutarate from general metabolism and proceeds via (R)-2-hydroxyglutarate, (R)-2-hydroxyglutar...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02049-10

    authors: Djurdjevic I,Zelder O,Buckel W

    更新日期:2011-01-01 00:00:00

  • Preferential binding of radiolabeled zearalenone to a protein fraction of Fusarium roseum graminearum.

    abstract::Zearalenone [6-(10-hydroxy-6-oxo-trans-1-undecenyl)beta-resorcyclic acid lactone] is a hormone produced by Fusarium spp. which regulates the sexual stage in F. roseum. 3H- and 14C-labeled zearalenone were found to bind preferentially to one of two peaks containing uncharacterized proteins obtained from the cytosol of ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.37.1.80-84.1979

    authors: Inaba T,Mirocha CJ

    更新日期:1979-01-01 00:00:00

  • Reduction of nitroaromatic compounds mediated by Streptomyces sp. exudates.

    abstract::Exudates from Streptomyces griseoflavus Tü 2484 effectively mediated electron transfer between hydrogen sulfide and various nitrobenzenes. In general, pseudo-first-order kinetics were observed, except for the initial phase of the reaction at higher pH values. Under fixed pH and Dh conditions, linear free energy relati...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.58.6.1945-1951.1992

    authors: Glaus MA,Heijman CG,Schwarzenbach RP,Zeyer J

    更新日期:1992-06-01 00:00:00

  • Deformed wing virus implicated in overwintering honeybee colony losses.

    abstract::The worldwide decline in honeybee colonies during the past 50 years has often been linked to the spread of the parasitic mite Varroa destructor and its interaction with certain honeybee viruses. Recently in the United States, dramatic honeybee losses (colony collapse disorder) have been reported; however, there remain...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02227-09

    authors: Highfield AC,El Nagar A,Mackinder LC,Noël LM,Hall MJ,Martin SJ,Schroeder DC

    更新日期:2009-11-01 00:00:00

  • Solute transport proteins and the outer membrane protein NmpC contribute to heat resistance of Escherichia coli AW1.7.

    abstract::This study aimed to elucidate determinants of heat resistance in Escherichia coli by comparing the composition of membrane lipids, as well as gene expression, in heat-resistant E. coli AW1.7 and heat-sensitive E. coli GGG10 with or without heat shock. The survival of E. coli AW1.7 at late exponential phase was 100-fol...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01930-10

    authors: Ruan L,Pleitner A,Gänzle MG,McMullen LM

    更新日期:2011-05-01 00:00:00

  • Demethylation of dimethylsulfoniopropionate to 3-mercaptopropionate by an aerobic marine bacterium.

    abstract::A bacterium, strain BIS-6, that grew aerobically on dimethylsulfoniopropionate (DMSP) was isolated from an intertidal mud sample. Strain BIS-6 quantitatively demethylated DMSP and 3-methiolpropionate to 3-mercaptopropionate. Strain BIS-6 was a versatile methylotroph growing on the osmolytes DMSP and glycine betaine an...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.60.12.4617-4619.1994

    authors: Visscher PT,Taylor BF

    更新日期:1994-12-01 00:00:00

  • Occurrence of toxicity among protease, amylase, and color mutants of a nontoxic soy sauce koji mold.

    abstract::A soy sauce koji mold, Aspergillus flavus var. columnaris Raper and Fennel (ATCC 44310), was treated with UV irradiation to obtain mutant strains possessing high protease activities, high amylase activities, and light-colored conidia. Selected mutant strains were tested for toxicity, and some were found acutely toxic ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.53.8.1980-1982.1987

    authors: Kalayanamitr A,Bhumiratana A,Flegel TW,Glinsukon T,Shinmyo A

    更新日期:1987-08-01 00:00:00

  • Gateway-based destination vectors for functional analyses of bacterial ORFeomes: application to the Min system in Brucella abortus.

    abstract::Twenty Gateway-compatible destination vectors were constructed. The vectors comprise fluorescent and epitope fusion tags, various drug markers, and replication origins that should make them useful for exploring existing microbial ORFeomes. In an attempt to validate several of these vectors, we observed polar and oscil...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01873-06

    authors: Hallez R,Letesson JJ,Vandenhaute J,De Bolle X

    更新日期:2007-02-01 00:00:00

  • A meta cleavage pathway for 4-chlorobenzoate, an intermediate in the metabolism of 4-chlorobiphenyl by Pseudomonas cepacia P166.

    abstract::Bacterial degradation of biphenyl and polychlorinated biphenyls proceeds by a well-studied pathway which produces benzoate and 2-hydroxypent-2,4-dienoate (or, in the case of polychlorinated biphenyls, the chlorinated derivatives of these compounds). Pseudomonas cepacia P166 utilizes 4-chlorobiphenyl for growth and pro...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.61.2.443-447.1995

    authors: Arensdorf JJ,Focht DD

    更新日期:1995-02-01 00:00:00

  • Propagation of Rhizopus javanicus Biosorbent.

    abstract::After propagation of Rhizopus javanicus in defined media containing glucose, urea, and mineral salts in deionized distilled water, the ability of the nonliving biomass to sequester cupric ion was assayed. Growth, uptake capacity (saturation uptake at >1 mM Cu concentration in solution), and biosorptive yield (biomass ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.48.1.137-141.1984

    authors: Treen-Sears ME,Martin SM,Volesky B

    更新日期:1984-07-01 00:00:00