Sulfur isotope enrichment during maintenance metabolism in the thermophilic sulfate-reducing bacterium Desulfotomaculum putei.

Abstract:

:Values of Delta(34)S (=delta(34)S(HS)-delta(34)S(SO(4)), where delta(34)S(HS) and delta(34)S(SO(4)) indicate the differences in the isotopic compositions of the HS(-) and SO(4)(2-) in the eluent, respectively) for many modern marine sediments are in the range of -55 to -75 per thousand, much greater than the -2 to -46 per thousand epsilon(34)S (kinetic isotope enrichment) values commonly observed for microbial sulfate reduction in laboratory batch culture and chemostat experiments. It has been proposed that at extremely low sulfate reduction rates under hypersulfidic conditions with a nonlimited supply of sulfate, isotopic enrichment in laboratory culture experiments should increase to the levels recorded in nature. We examined the effect of extremely low sulfate reduction rates and electron donor limitation on S isotope fractionation by culturing a thermophilic, sulfate-reducing bacterium, Desulfotomaculum putei, in a biomass-recycling culture vessel, or "retentostat." The cell-specific rate of sulfate reduction and the specific growth rate decreased progressively from the exponential phase to the maintenance phase, yielding average maintenance coefficients of 10(-16) to 10(-18) mol of SO(4) cell(-1) h(-1) toward the end of the experiments. Overall S mass and isotopic balance were conserved during the experiment. The differences in the delta(34)S values of the sulfate and sulfide eluting from the retentostat were significantly larger, attaining a maximum Delta(34)S of -20.9 per thousand, than the -9.7 per thousand observed during the batch culture experiment, but differences did not attain the values observed in marine sediments.

journal_name

Appl Environ Microbiol

authors

Davidson MM,Bisher ME,Pratt LM,Fong J,Southam G,Pfiffner SM,Reches Z,Onstott TC

doi

10.1128/AEM.02948-08

subject

Has Abstract

pub_date

2009-09-01 00:00:00

pages

5621-30

issue

17

eissn

0099-2240

issn

1098-5336

pii

AEM.02948-08

journal_volume

75

pub_type

杂志文章
  • Novel bifunctional hyperthermostable carboxypeptidase/aminoacylase from Pyrococcus horikoshii OT3.

    abstract::Genome sequencing of the thermophilic archaeon Pyrococcus horikoshii OT3 revealed a gene which had high sequence similarity to the gene encoding the carboxypeptidase of Sulfolobus solfataricus and also to that encoding the aminoacylase from Bacillus stearothermophilus. The gene from P. horikoshii comprises an open rea...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.67.2.673-679.2001

    authors: Ishikawa K,Ishida H,Matsui I,Kawarabayasi Y,Kikuchi H

    更新日期:2001-02-01 00:00:00

  • Molecular and genetic characterization of propionicin F, a bacteriocin from Propionibacterium freudenreichii.

    abstract::This work describes the purification and characterization of propionicin F, the first bacteriocin isolated from Propionibacterium freudenreichii. The bacteriocin has a bactericidal activity and is only active against strains of P. freudenreichii. Propionicin F appears to be formed through a processing pathway new to b...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.70.12.7303-7310.2004

    authors: Brede DA,Faye T,Johnsborg O,Odegård I,Nes IF,Holo H

    更新日期:2004-12-01 00:00:00

  • Clinically Unreported Salmonellosis Outbreak Detected via Comparative Genomic Analysis of Municipal Wastewater Salmonella Isolates.

    abstract::Municipal wastewater includes human waste that contains both commensal and pathogenic enteric microorganisms, and this collective community microbiome can be monitored for community diseases. In a previous study, we assessed the salmonellosis disease burden using municipal wastewater from Honolulu, Hawaii, which was m...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00139-19

    authors: Diemert S,Yan T

    更新日期:2019-05-02 00:00:00

  • Sensitivity of environmental microorganisms to antimicrobial agents.

    abstract::The sensitivity of different microorganisms, considered as typical representatives of the microflora of soil and water, was established to evaluate the influence of the nonmedical use of antimicrobial agents on bacterial ecology. Only seven strains, six chemoorganotrophs and one chemolithotroph, could be considered as...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.31.3.332-336.1976

    authors: Van Dijck P,van de Voorde H

    更新日期:1976-03-01 00:00:00

  • Hypoxic adaptation by Efg1 regulates biofilm formation by Candida albicans.

    abstract::Hypoxia is encountered frequently by Candida albicans during systemic infection of the human host. We tested if hypoxia allows biofilm formation by C. albicans, which is a major cause of perseverance and antifungal resistance in C. albicans infections. Using an in vitro biofilm system, we unexpectedly discovered that ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00098-09

    authors: Stichternoth C,Ernst JF

    更新日期:2009-06-01 00:00:00

  • BadR and BadM Proteins Transcriptionally Regulate Two Operons Needed for Anaerobic Benzoate Degradation by Rhodopseudomonas palustris.

    abstract::The bacterium Rhodopseudomonas palustris grows with the aromatic acid benzoate and the alicyclic acid cyclohexanecarboxylate (CHC) as sole carbon sources. The enzymatic steps in an oxygen-independent pathway for CHC degradation have been elucidated, but it was unknown how the CHC operon (badHI aliAB badK) encoding the...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00377-15

    authors: Hirakawa H,Hirakawa Y,Greenberg EP,Harwood CS

    更新日期:2015-07-01 00:00:00

  • Microbiota-Mediated Modulation of Organophosphate Insecticide Toxicity by Species-Dependent Interactions with Lactobacilli in a Drosophila melanogaster Insect Model.

    abstract::Despite the benefits to the global food supply and agricultural economies, pesticides are believed to pose a threat to the health of both humans and wildlife. Chlorpyrifos (CP), a commonly used organophosphate insecticide, has poor target specificity and causes acute neurotoxicity in a wide range of species via the su...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02820-17

    authors: Daisley BA,Trinder M,McDowell TW,Collins SL,Sumarah MW,Reid G

    更新日期:2018-04-16 00:00:00

  • Reduction of Escherichia coli O157:H7 populations in cattle by addition of colicin E7-producing E. coli to feed.

    abstract::A cattle trial using artificially inoculated calves was conducted to determine the effect of the addition of colicinogenic Escherichia coli strains capable of producing colicin E7 (a 61-kDa DNase) to feed on the fecal shedding of serotype O157:H7. The experiment was divided into three periods. In period 1, which laste...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.70.10.6053-6060.2004

    authors: Schamberger GP,Phillips RL,Jacobs JL,Diez-Gonzalez F

    更新日期:2004-10-01 00:00:00

  • Genome-driven investigation of compatible solute biosynthesis pathways of Pseudomonas syringae pv. syringae and their contribution to water stress tolerance.

    abstract::The foliar pathogen Pseudomonas syringae pv. syringae exhibits an exceptional ability to survive on asymptomatic plants as an epiphyte. Intermittent wetting events on plants lead to osmotic and matric stresses which must be tolerated for survival as an epiphyte. In this study, we have applied bioinformatic, genetic, a...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00686-10

    authors: Kurz M,Burch AY,Seip B,Lindow SE,Gross H

    更新日期:2010-08-01 00:00:00

  • Production of high concentrations of ethanol from inulin by simultaneous saccharification and fermentation using Aspergillus niger and Saccharomyces cerevisiae.

    abstract::Pure nonhydrolyzed inulin was directly converted to ethanol in a simultaneous saccharification and fermentation process. An inulinase-hyperproducing mutant, Aspergillus niger 817, was grown in a submerged culture at 30 degrees C for 5 days. The inulin-digestive liquid culture (150 ml) was supplemented with 45 g of inu...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.59.3.729-733.1993

    authors: Ohta K,Hamada S,Nakamura T

    更新日期:1993-03-01 00:00:00

  • Ecophysiology of "Halarsenatibacter silvermanii" strain SLAS-1T, gen. nov., sp. nov., a facultative chemoautotrophic arsenate respirer from salt-saturated Searles Lake, California.

    abstract::Searles Lake occupies a closed basin harboring salt-saturated, alkaline brines that have exceptionally high concentrations of arsenic oxyanions. Strain SLAS-1(T) was previously isolated from Searles Lake (R. S. Oremland, T. R. Kulp, J. Switzer Blum, S. E. Hoeft, S. Baesman, L. G. Miller, and J. F. Stolz, Science 308:1...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02614-08

    authors: Blum JS,Han S,Lanoil B,Saltikov C,Witte B,Tabita FR,Langley S,Beveridge TJ,Jahnke L,Oremland RS

    更新日期:2009-04-01 00:00:00

  • Light as an energy source in continuous cultures of bacteriorhodopsin-containing halobacteria.

    abstract::The role of light as an energy source for slightly aereated cultures of halobacteria was studied, using continuous cultures with low nutrient concentrations and a low oxygen supply. A series of experiments were carried out with non-illuminated and differently illuminated cultures and with different oxygen transfer rat...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.45.3.868-871.1983

    authors: Rodriguez-Valera F,Nieto JJ,Ruiz-Berraquero F

    更新日期:1983-03-01 00:00:00

  • Measurement and significance of specific activity in the heterotrophic bacteria of natural waters.

    abstract::It is now possible to obtain accurate total counts of the bacteria of natural waters with the use of acridine orange staining and epifluorescence microscopy. This approach can be coupled to highly sensitive measurements of heterotrophic activity using radioisotopes. To accomplish this, three variations of a "specific ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.36.2.297-305.1978

    authors: Wright RT

    更新日期:1978-08-01 00:00:00

  • Kinetics of Insoluble Cellulose Fermentation by Continuous Cultures of Ruminococcus albus.

    abstract::Data from analyses of continuous culture fermentation of insoluble cellulose by Ruminococcus albus 7 were used to derive constants for the rate of cellulose hydrolysis and fermentation, growth yield, and maintenance. Cellulose concentration was 1% in the nutrient reservoir, and hydraulic retention times of 0.5, 1.0, 1...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.54.11.2660-2663.1988

    authors: Pavlostathis SG,Miller TL,Wolin MJ

    更新日期:1988-11-01 00:00:00

  • Detection of tetracycline resistance determinants in pig isolates from three herds with different histories of antimicrobial agent exposure.

    abstract::A total of 114 gram-negative fecal isolates from domestic pigs in herds with different histories of antimicrobial agent exposure were screened for the presence of plasmid DNA and specific tetracycline resistance determinants. More than 84% of the isolates harbored plasmid DNA, which ranged in size from 2.1 to 186 kb. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.59.5.1467-1472.1993

    authors: Lee C,Langlois BE,Dawson KA

    更新日期:1993-05-01 00:00:00

  • Characterization of Pseudomonas aeruginosa bacteriophage UNL-1, a bacterial virus with a novel UV-A-inducible DNA damage reactivation phenotype.

    abstract::UNL-1, a lytic virus of Pseudomonas aeruginosa, was observed to express a novel inducible DNA damage reactivation activity in UV-A-irradiated P. aeruginosa host cells. The expression of bacteriophage reactivation was quantified in hosts exposed to either UV-C or UV-A radiation. While reactivation of UV-C-damaged UNL-1...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.65.6.2606-2613.1999

    authors: Shaffer JJ,Jacobsen LM,Schrader JO,Lee KW,Martin EL,Kokjohn TA

    更新日期:1999-06-01 00:00:00

  • Trifolitoxin Production Increases Nodulation Competitiveness of Rhizobium etli CE3 under Agricultural Conditions.

    abstract::A major barrier to the use of nitrogen-fixing inoculum strains for the enhancement of legume productivity is the inability of commercially available strains to compete with indigenous rhizobia for nodule formation. Despite extensive research on nodulation competitiveness, there are no examples of field efficacy studie...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.64.7.2630-2633.1998

    authors: Robleto EA,Kmiecik K,Oplinger ES,Nienhuis J,Triplett EW

    更新日期:1998-07-01 00:00:00

  • A patatin-like protein associated with the polyhydroxyalkanoate (PHA) granules of Haloferax mediterranei acts as an efficient depolymerase in the degradation of native PHA.

    abstract::The key enzymes and pathways involved in polyhydroxyalkanoate (PHA) biosynthesis in haloarchaea have been identified in recent years, but the haloarchaeal enzymes for PHA degradation remain unknown. In this study, a patatin-like PHA depolymerase, PhaZh1, was determined to be located on the PHA granules in the haloarch...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.04269-14

    authors: Liu G,Hou J,Cai S,Zhao D,Cai L,Han J,Zhou J,Xiang H

    更新日期:2015-05-01 00:00:00

  • Spoilage association of chicken skin.

    abstract::The bacterial succession on the skin of broiler chicken carcasses stored at 2 degrees C was traced, and the ability of representative isolates to produce off-odors was determined by using sterile leg and breast muscle sections. Off-odors were identified by olfactory and chemical means. The inability of peptone-iron ag...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.37.3.399-401.1979

    authors: Daud HB,McMeekin TA,Thomas CJ

    更新日期:1979-03-01 00:00:00

  • Survival of Vibrio cholerae and Escherichia coli in estuarine waters and sediments.

    abstract::In in vitro estuarine water and sediment chambers, the survival of Vibrio cholerae and Escherichia coli was determined by plate counting and direct counting techniques. V. cholerae strains included environmental, clinical, and serotype O1 and non-O1 isolates, whereas E. coli strains included ATCC 25922 and a freshly c...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.43.3.578-584.1982

    authors: Hood MA,Ness GE

    更新日期:1982-03-01 00:00:00

  • Characterization of 16S rRNA genes from oil field microbial communities indicates the presence of a variety of sulfate-reducing, fermentative, and sulfide-oxidizing bacteria.

    abstract::Oil field bacteria were characterized by cloning and sequencing of PCR-amplified 16S rRNA genes. A variety of gram-negative, sulfate-reducing bacteria was detected (16 members of the family Desulfovibrionaceae and 8 members of the family Desulfobacteriaceae). In contrast, a much more limited number of anaerobic, ferme...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.62.5.1623-1629.1996

    authors: Voordouw G,Armstrong SM,Reimer MF,Fouts B,Telang AJ,Shen Y,Gevertz D

    更新日期:1996-05-01 00:00:00

  • Interaction of Ammonia Monooxygenase from Nitrosomonas europaea with Alkanes, Alkenes, and Alkynes.

    abstract::Ammonia monooxygenase of Nitrosomonas europaea catalyzes the oxidation of alkanes (up to C(8)) to alcohols and alkenes (up to C(5)) to epoxides and alcohols in the presence of ammonium ions. Straight-chain, N-terminal alkynes (up to C(10)) all exhibited a time-dependent inhibition of ammonia oxidation without effects ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.54.12.3187-3190.1988

    authors: Hyman MR,Murton IB,Arp DJ

    更新日期:1988-12-01 00:00:00

  • Identification of DysI, the immunity factor of the streptococcal bacteriocin dysgalacticin.

    abstract::DysI is identified as the protein that confers specific immunity to dysgalacticin, a plasmid-encoded streptococcal bacteriocin. dysI is transcribed as part of the copG-repB-dysI replication-associated operon. DysI appears to function at the membrane level to prevent the inhibitory effects of dysgalacticin on glucose t...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01707-10

    authors: Swe PM,Heng NC,Cook GM,Tagg JR,Jack RW

    更新日期:2010-12-01 00:00:00

  • Detection of microbial growth on polycyclic aromatic hydrocarbons in microtiter plates by using the respiration indicator WST-1.

    abstract::We have developed a microtiter plate method for screening a large number of bacterial isolates for the ability to grow on different crystalline polycyclic aromatic hydrocarbons (PAHs). Growth on PAHs cannot easily be determined with standard growth assays because of the very low aqueous solubility and bioavailability ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.68.6.2683-2689.2002

    authors: Johnsen AR,Bendixen K,Karlson U

    更新日期:2002-06-01 00:00:00

  • Ultrastructure and Host Specificity of Bacteriophages of Streptococcus cremoris, Streptococcus lactis subsp. diacetylactis, and Leuconostoc cremoris from Finnish Fermented Milk "Viili".

    abstract::"Viili," a fermented milk product, has a firm but viscous consistency. It is produced with traditional mesophilic mixed-strain starters, which have various stabilities in dairy practice. Thirteen morphologically different types of phages were found in 90 viili samples studied by electron microscopy. Ten of the phage t...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.52.4.771-777.1986

    authors: Saxelin ML,Nurmiaho-Lassila EL,Meriläinen VT,Forsén RI

    更新日期:1986-10-01 00:00:00

  • Selection of Pseudomonas sp. strain HBP1 Prp for metabolism of 2-propylphenol and elucidation of the degradative pathway.

    abstract::A mutant of Pseudomonas sp. strain HBP1, originally isolated on 2-hydroxybiphenyl, was selected for the ability to grow on 2-propylphenol as the sole carbon and energy source. In the mutant strain, which was designated as Pseudomonas sp. strain HBP1 Prp, the cellular induction mechanism involved in the synthesis of th...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.59.3.860-866.1993

    authors: Kohler HP,van der Maarel MJ,Kohler-Staub D

    更新日期:1993-03-01 00:00:00

  • Boosting Secretion of Extracellular Protein by Escherichia coli via Cell Wall Perturbation.

    abstract::Escherichia coli is one of the most widely used host microorganisms for recombinant protein expression and metabolic engineering, but it cannot efficiently secrete recombinant proteins to extracellular space. Here, extracellular protein secretion was enhanced in E. coli by deleting two d,d-carboxypeptidase genes (dacA...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01382-18

    authors: Yang H,Lu X,Hu J,Chen Y,Shen W,Liu L

    更新日期:2018-10-01 00:00:00

  • Association with an ammonium-excreting bacterium allows diazotrophic culture of oil-rich eukaryotic microalgae.

    abstract::Concerns regarding the depletion of the world's reserves of oil and global climate change have promoted an intensification of research and development toward the production of biofuels and other alternative sources of energy during the last years. There is currently much interest in developing the technology for third...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.06260-11

    authors: Ortiz-Marquez JC,Do Nascimento M,Dublan Mde L,Curatti L

    更新日期:2012-04-01 00:00:00

  • Seasonal cholera caused by Vibrio cholerae serogroups O1 and O139 in the coastal aquatic environment of Bangladesh.

    abstract::Since Vibrio cholerae O139 first appeared in 1992, both O1 El Tor and O139 have been recognized as the epidemic serogroups, although their geographic distribution, endemicity, and reservoir are not fully understood. To address this lack of information, a study of the epidemiology and ecology of V. cholerae O1 and O139...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00066-06

    authors: Alam M,Hasan NA,Sadique A,Bhuiyan NA,Ahmed KU,Nusrin S,Nair GB,Siddique AK,Sack RB,Sack DA,Huq A,Colwell RR

    更新日期:2006-06-01 00:00:00

  • Anaerobic ortho Dechlorination of Polychlorinated Biphenyls by Estuarine Sediments from Baltimore Harbor.

    abstract::Reductive dechlorination of the ortho moiety of polychlorinated biphenyls (PCBs) as well as of meta and para moieties is shown to occur in anaerobic enrichments of Baltimore Harbor sediments. These estuarine sediments ortho dechlorinated 2,3,5,6-chlorinated biphenyl (CB), 2,3,5-CB, and 2,3,6-CB in freshwater or estuar...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.62.7.2534-2539.1996

    authors: Berkaw M,Sowers KR,May HD

    更新日期:1996-07-01 00:00:00