Selection of Pseudomonas sp. strain HBP1 Prp for metabolism of 2-propylphenol and elucidation of the degradative pathway.

Abstract:

:A mutant of Pseudomonas sp. strain HBP1, originally isolated on 2-hydroxybiphenyl, was selected for the ability to grow on 2-propylphenol as the sole carbon and energy source. In the mutant strain, which was designated as Pseudomonas sp. strain HBP1 Prp, the cellular induction mechanism involved in the synthesis of the NADH-dependent monooxygenase is changed. 2-Propylphenol, which is known to be a substrate of the monooxygenase, does not induce formation of the monooxygenase in the wild type but does have an induction effect in the mutant strain. Furthermore, in contrast to the wild type, mutant strain HBP1 Prp constitutively produces a small amount of monooxygenase and metapyrocatechase. The enzymes from strain HBP1 Prp catalyzing the first three steps in the degradation of 2-propylphenol--the NADH-dependent monooxygenase, the metapyrocatechase, and the meta fission product hydrolase--were partially purified, and their activities were measured. The product of the monooxygenase activity was identified by mass spectrometry as 3-propylcatechol. The metapyrocatechase used this compound as a substrate and produced a yellow meta fission product that was identified by mass spectrometry as 2-hydroxy-6-oxo-nona-2,4- dienoate. Butyrate could be detected as a product of the meta fission product hydrolase in crude cell extract of 2-propylphenol-grown cells, as well as an intermediate in culture broths during growth on 2-propylphenol. All three enzymes expressed highest activities for the metabolites of the degradation of 2-hydroxybiphenyl.

journal_name

Appl Environ Microbiol

authors

Kohler HP,van der Maarel MJ,Kohler-Staub D

doi

10.1128/AEM.59.3.860-866.1993

subject

Has Abstract

pub_date

1993-03-01 00:00:00

pages

860-6

issue

3

eissn

0099-2240

issn

1098-5336

journal_volume

59

pub_type

杂志文章
  • Genetic Diversity of Flavobacterium psychrophilum Isolates from Three Oncorhynchus spp. in the United States, as Revealed by Multilocus Sequence Typing.

    abstract:UNLABELLED:The use of a multilocus sequence typing (MLST) technique has identified the intraspecific genetic diversity of U.S. Flavobacterium psychrophilum, an important pathogen of salmonids worldwide. Prior to this analysis, little U.S. F. psychrophilum genetic information was known; this is of importance when consid...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00411-16

    authors: Van Vliet D,Wiens GD,Loch TP,Nicolas P,Faisal M

    更新日期:2016-05-16 00:00:00

  • Enteric Viruses in Surface Waters from Argentina: Molecular and Viable-Virus Detection.

    abstract::Water resources contaminated with wastewater are an important source for the dissemination of enteric viruses with an impact on the health of the population. The aim of the study was to assess the viral contamination of freshwater from a dam in Argentina by using infectious enterovirus detection, viral RNA amplificati...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02327-17

    authors: Masachessi G,Pisano MB,Prez VE,Martínez LC,Michelena JF,Martínez-Wassaf M,Giordano MO,Isa MB,Pavan JV,Welter A,Nates SV,Ré V

    更新日期:2018-02-14 00:00:00

  • Proline reverses the abnormal phenotypes of Colletotrichum trifolii associated with expression of endogenous constitutively active Ras.

    abstract::Colletotrichum trifolii is the causative organism of alfalfa anthracnose. We previously cloned and characterized the small prototypical G protein, Ras, of C. trifolii, which is involved in the signaling pathways that mediate interaction between the pathogen and its host. Transformants expressing constitutively active ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.68.4.1647-1651.2002

    authors: Memmott SD,Ha YS,Dickman MB

    更新日期:2002-04-01 00:00:00

  • Effects of cadmium, copper, magnesium, and zinc on the decomposition of citrate by a Klebsiella sp.

    abstract::The effects of Cd2+, Cu2+, Mg2+, and Zn2+ on the decomposition of citric acid by a Klebsiella sp. were studied by monitoring the degradation of [14C]citrate. The carbon concentration used was 10 micrograms of C liter-1, and the media were designed to provide at least 95% of the citrate complexed to the metal studied. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.55.6.1375-1379.1989

    authors: Brynhildsen L,Rosswall T

    更新日期:1989-06-01 00:00:00

  • Effect of 3-Phenylpropanoic Acid on Capsule and Cellulases of Ruminococcus albus 8.

    abstract::The morphology and cellulases of Ruminococcus albus 8 were markedly affected by the inclusion of 3-phenylpropanoic acid (PPA) in a defined growth medium. PPA-grown bacteria produced substantial quantities of cell-bound cellulase, as well as a very high-molecular-weight extracellular enzyme and lesser amounts of two lo...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.48.1.218-223.1984

    authors: Stack RJ,Hungate RE

    更新日期:1984-07-01 00:00:00

  • Restriction endonuclease analysis to distinguish two closely related nuclear polyhedrosis viruses: Autographa californica MNPV and Trichoplusia ni MNPV.

    abstract::Restriction endonuclease fragment patterns of the deoxyribonucleic acid genomes of Autographa californica nuclear polyhedrosis virus (multiply embedded type) and Trichoplusia ni nuclear polyhedrosis virus (multiply embedded type) demonstrate that the two viruses are distinct but closely related variants. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.35.6.1206-1210.1978

    authors: Miller LK,Dawes KP

    更新日期:1978-06-01 00:00:00

  • Viral density and virus-to-bacterium ratio in deep-sea sediments of the Eastern Mediterranean.

    abstract::Viruses are now recognized as a key component in pelagic systems, but their role in marine sediment has yet to be assessed. In this study bacterial and viral densities were determined at nine deep-sea stations selected from three main sites (i.e., the Sporades Basin, the Cretan Sea, and the Ierapetra Trench at depths ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.66.5.1857-1861.2000

    authors: Danovaro R,Serresi M

    更新日期:2000-05-01 00:00:00

  • Chelating Properties of Extracellular Polysaccharides from Chlorella spp.

    abstract::Chlorella stigmatophora LB 993 was grown in artificial seawater under controlled conditions. The production of cell wall polysaccharides attached to the cells and dissolved in the growth medium was monitored during algal growth. Preliminary characterization of the dissolved polysaccharides of C. stigmatophora and othe...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.53.12.2953-2956.1987

    authors: Kaplan D,Christiaen D,Arad SM

    更新日期:1987-12-01 00:00:00

  • Characterization of site-specific mutations in a short-chain-length/medium-chain-length polyhydroxyalkanoate synthase: in vivo and in vitro studies of enzymatic activity and substrate specificity.

    abstract::Saturation point mutagenesis was carried out at position 479 in the polyhydroxyalkanoate (PHA) synthase from Chromobacterium sp. strain USM2 (PhaC(Cs)) with specificities for short-chain-length (SCL) [(R)-3-hydroxybutyrate (3HB) and (R)-3-hydroxyvalerate (3HV)] and medium-chain-length (MCL) [(R)-3-hydroxyhexanoate (3H...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00564-13

    authors: Chuah JA,Tomizawa S,Yamada M,Tsuge T,Doi Y,Sudesh K,Numata K

    更新日期:2013-06-01 00:00:00

  • Successive mineralization and detoxification of benzo[a]pyrene by the white rot fungus Bjerkandera sp. strain BOS55 and indigenous microflora.

    abstract::White rot fungi can oxidize high-molecular-weight polycyclic aromatic hydrocarbons (PAH) rapidly to polar metabolites, but only limited mineralization takes place. The objectives of this study were to determine if the polar metabolites can be readily mineralized by indigenous microflora from several inoculum sources, ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.64.8.2853-2858.1998

    authors: Kotterman MJ,Vis EH,Field JA

    更新日期:1998-08-01 00:00:00

  • Ecological constraints limit the fitness of fungal hybrids in the Heterobasidion annosum species complex.

    abstract::The ability of two closely related species to maintain species boundaries in spite of retained interfertility between them is a documented driving force of speciation. Experimental evidence to support possible interspecific postzygotic isolation mechanisms for organisms belonging to the kingdom Fungi is still missing....

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01135-07

    authors: Garbelotto M,Gonthier P,Nicolotti G

    更新日期:2007-10-01 00:00:00

  • Elevated temperature enhances virulence of Erwinia carotovora subsp. carotovora strain EC153 to plants and stimulates production of the quorum sensing signal, N-acyl homoserine lactone, and extracellular proteins.

    abstract::Erwinia carotovora subsp. atroseptica, E. carotovora subsp. betavasculorum, and E. carotovora subsp. carotovora produce high levels of extracellular enzymes, such as pectate lyase (Pel), polygalacturonase (Peh), cellulase (Cel), and protease (Prt), and the quorum-sensing signal N-acyl-homoserine lactone (AHL) at 28 de...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.71.8.4655-4663.2005

    authors: Hasegawa H,Chatterjee A,Cui Y,Chatterjee AK

    更新日期:2005-08-01 00:00:00

  • Copper Reduction and Contact Killing of Bacteria by Iron Surfaces.

    abstract::The well-established killing of bacteria by copper surfaces, also called contact killing, is currently believed to be a combined effect of bacterial contact with the copper surface and the dissolution of copper, resulting in lethal bacterial damage. Iron can similarly be released in ionic form from iron surfaces and w...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01725-15

    authors: Mathews S,Kumar R,Solioz M

    更新日期:2015-09-01 00:00:00

  • Cytotoxicity of T-2 toxin and its metabolites determined with the neutral red cell viability assay.

    abstract::The neutral red (NR) cell viability assay was used with various cell types of human origin to quantitate the potency of T-2 mycotoxin and its metabolites. The human melanoma SK-Mel/27 cell line was the most sensitive, with a midpoint cytotoxicity value of 2.8 ng of T-2 per ml. With the human hepatoma cell line, HepG2,...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.57.7.2101-2103.1991

    authors: Babich H,Borenfreund E

    更新日期:1991-07-01 00:00:00

  • Transient marker system for iterative gene targeting of a prototrophic fungus.

    abstract::Auxotrophic microorganisms are often used for genetic engineering, because their biosynthetic deficiency can be complemented by the transforming DNA and allows selection for transformants that have become prototrophic. However, when complementation is obtained by ectopic expression this may lead to unpredictable side ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01839-07

    authors: Nielsen ML,de Jongh WA,Meijer SL,Nielsen J,Mortensen UH

    更新日期:2007-11-01 00:00:00

  • Differentiation of strains of Xylella fastidiosa by a variable number of tandem repeat analysis.

    abstract::Short sequence repeats (SSRs) with a potential variable number of tandem repeat (VNTR) loci were identified in the genome of the citrus pathogen Xylella fastidiosa and used for typing studies. Although mono- and dinucleotide repeats were absent, we found several intermediate-length 7-, 8-, and 9-nucleotide repeats, wh...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.67.9.4091-4095.2001

    authors: Coletta-Filho HD,Takita MA,de Souza AA,Aguilar-Vildoso CI,Machado MA

    更新日期:2001-09-01 00:00:00

  • Ectomycorrhizal Fungal Communities in Urban Parks Are Similar to Those in Natural Forests but Shaped by Vegetation and Park Age.

    abstract::Ectomycorrhizal (ECM) fungi are important mutualists for the growth and health of most boreal trees. Forest age and its host species composition can impact the composition of ECM fungal communities. Although plentiful empirical data exist for forested environments, the effects of established vegetation and its success...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01797-17

    authors: Hui N,Liu X,Kotze DJ,Jumpponen A,Francini G,Setälä H

    更新日期:2017-11-16 00:00:00

  • Production of trypsin inhibitor by a Cephalosporium sp.

    abstract::Conditions for the production of the trypsin inhibitor from Cephalosporium sp. KM 388 were investigated. Polypeptone-meat extract-glucose medium supported excellent production of the trypsin inhibitor. In this medium, polypeptone and meat extract were utilized both as carbon and nitrogen sources and as limiting substr...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.35.4.631-635.1978

    authors: Tsuchiya K,Kimura T

    更新日期:1978-04-01 00:00:00

  • Degradation of bromacil by a Pseudomonas sp.

    abstract::A gram-negative rod, identified as a Pseudomonas sp., was isolated from soil by using bromacil as the sole source of carbon and energy. During growth on bromacil or 5-bromouracil, almost stoichiometric amounts of bromide were released. The bacterium was shown to harbor two plasmids approximately 60 and 100 kilobases i...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.54.9.2203-2207.1988

    authors: Chaudhry GR,Cortez L

    更新日期:1988-09-01 00:00:00

  • Role of BGS13 in the Secretory Mechanism of Pichia pastoris.

    abstract::The methylotrophic yeast Pichia pastoris has been utilized for heterologous protein expression for over 30 years. Because P. pastoris secretes few of its own proteins, the exported recombinant protein is the major polypeptide in the extracellular medium, making purification relatively easy. Unfortunately, some recombi...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01615-19

    authors: Naranjo CA,Jivan AD,Vo MN,de Sa Campos KH,Deyarmin JS,Hekman RM,Uribe C,Hang A,Her K,Fong MM,Choi JJ,Chou C,Rabara TR,Myers G,Moua P,Thor,Risser DD,Vierra CA,Franz AH,Lin-Cereghino J,Lin-Cereghino GP

    更新日期:2019-11-27 00:00:00

  • Effect of organotins on fecal pollution indicator organisms.

    abstract::Pure cultures of Escherichia coli and Streptococcus faecalis and environmental water samples were examined for the possibility that pollution involving organotin compounds could decrease the values for indicator organisms when standard methods were applied to the analysis of water samples. (CH3)2SnCl2 and (CH3)3SnCl d...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.52.3.562-566.1986

    authors: Pettibone GW,Cooney JJ

    更新日期:1986-09-01 00:00:00

  • Glycosidases of the rumen anaerobic fungus Neocallimastix frontalis grown on cellulosic substrates.

    abstract::The rumen anaerobic fungus Neocallimastix frontalis was grown on cellulosic substrates, and the cellular distribution and types of glycosidases produced by the organism were studied. Fungal cultures were fractionated into extracellular, insoluble (membrane), and intracellular fractions and assayed for glycosidase acti...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.49.5.1265-1269.1985

    authors: Pearce PD,Bauchop T

    更新日期:1985-05-01 00:00:00

  • "Endomicrobia": cytoplasmic symbionts of termite gut protozoa form a separate phylum of prokaryotes.

    abstract::Lignocellulose digestion by wood-feeding termites depends on the mutualistic interaction of unusual, flagellate protists located in their hindgut. Most of the flagellates harbor numerous prokaryotic endosymbionts of so-far-unknown identity and function. Using a full-cycle molecular approach, we show here that the endo...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.71.3.1473-1479.2005

    authors: Stingl U,Radek R,Yang H,Brune A

    更新日期:2005-03-01 00:00:00

  • GlcNAc De-N-Acetylase from the Hyperthermophilic Archaeon Sulfolobus solfataricus.

    abstract::Sulfolobus solfataricus is an aerobic crenarchaeal hyperthermophile with optimum growth at temperatures greater than 80°C and pH 2 to 4. Within the crenarchaeal group of Sulfolobales, N-acetylglucosamine (GlcNAc) has been shown to be a component of exopolysaccharides, forming their biofilms, and of the N-glycan decora...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01879-18

    authors: Iacono R,Strazzulli A,Maurelli L,Curci N,Casillo A,Corsaro MM,Moracci M,Cobucci-Ponzano B

    更新日期:2019-01-09 00:00:00

  • Nonnodulating Bradyrhizobium spp. Modulate the Benefits of Legume-Rhizobium Mutualism.

    abstract:UNLABELLED:Rhizobia are best known for nodulating legume roots and fixing atmospheric nitrogen for the host in exchange for photosynthates. However, the majority of the diverse strains of rhizobia do not form nodules on legumes, often because they lack key loci that are needed to induce nodulation. Nonnodulating rhizob...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01116-16

    authors: Gano-Cohen KA,Stokes PJ,Blanton MA,Wendlandt CE,Hollowell AC,Regus JU,Kim D,Patel S,Pahua VJ,Sachs JL

    更新日期:2016-08-15 00:00:00

  • Propachlor removal by Pseudomonas strain GCH1 in an immobilized-cell system.

    abstract::A bacterial strain capable of growing on propachlor (2-chloro-N-isopropylacetanilide) was isolated from soil by using enrichment and isolation techniques. The strain isolated, designated GCH1, was classified as a member of the genus Pseudomonas. Washed-cell suspensions of strain GCH1 accumulated N-isopropylacetanilide...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.66.3.1190-1194.2000

    authors: Martín M,Mengs G,Plaza E,Garbi C,Sánchez M,Gibello A,Gutierrez F,Ferrer E

    更新日期:2000-03-01 00:00:00

  • Genetic diversity and relationships among isolates of Rhizobium leguminosarum biovar phaseoli.

    abstract::Fifty-one isolates of Rhizobium leguminosarum biovar phaseoli from various geographic and ecological sources, largely in Mexico, were characterized by the electrophoretic mobilities of 15 metabolic enzymes, and 46 distinctive multilocus genotypes (electrophoretic types [ETs]) were distinguished on the basis of allele ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.54.11.2825-2832.1988

    authors: Pinero D,Martinez E,Selander RK

    更新日期:1988-11-01 00:00:00

  • Controlled gene expression in the plant pathogen Cryphonectria parasitica by use of a copper-responsive element.

    abstract::We have developed a tool for controlled expression of heterologous or ectopic genes in the chestnut pathogen Cryphonectria parasitica using the promoter region from a putative copper-regulated transporter gene. In addition, we have found that expression control via this system is not affected by the virulence-attenuat...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00899-09

    authors: Willyerd KL,Kemp AM,Dawe AL

    更新日期:2009-08-01 00:00:00

  • Thermophilic methanogenesis in a hot-spring algal-bacterial mat (71 to 30 degrees C).

    abstract::Algal-bacterial mats which grow in the effluent channels of alkaline hot springs provided an environment suitable for studying natural thermophilic methane producing bacteria. Methane was rapidly produced in cores taken from the meat and appeared to be an end product of decomposition of the algal-bacterial organic mat...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.35.6.1019-1026.1978

    authors: Ward DM

    更新日期:1978-06-01 00:00:00

  • AcrR and Rex Control Mannitol and Sorbitol Utilization through Their Cross-Regulation of Aldehyde-Alcohol Dehydrogenase (AdhE) in Lactobacillus plantarum.

    abstract::Lactobacillus plantarum is a versatile bacterium that occupies a wide range of environmental niches. In this study, we found that a bifunctional aldehyde-alcohol dehydrogenase-encoding gene, adhE, was responsible for L. plantarum being able to utilize mannitol and sorbitol through cross-regulation by two DNA-binding r...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02035-18

    authors: Yang X,Teng K,Su R,Li L,Zhang T,Fan K,Zhang J,Zhong J

    更新日期:2019-02-06 00:00:00