Preferential binding of radiolabeled zearalenone to a protein fraction of Fusarium roseum graminearum.

Abstract:

:Zearalenone [6-(10-hydroxy-6-oxo-trans-1-undecenyl)beta-resorcyclic acid lactone] is a hormone produced by Fusarium spp. which regulates the sexual stage in F. roseum. 3H- and 14C-labeled zearalenone were found to bind preferentially to one of two peaks containing uncharacterized proteins obtained from the cytosol of young mycelium and resolved by gel column chromatography. The proteins were partially purified by successive resolution on Sephadex G-100, Sephadex G-200, and BioGel P-300. Free zearalenone (37%) was reisolated from the purified proteins after resolution by thin-layer chromatography or partitioning with ethyl acetate.

journal_name

Appl Environ Microbiol

authors

Inaba T,Mirocha CJ

doi

10.1128/AEM.37.1.80-84.1979

subject

Has Abstract

pub_date

1979-01-01 00:00:00

pages

80-4

issue

1

eissn

0099-2240

issn

1098-5336

journal_volume

37

pub_type

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