Diversity of Salmonella strains isolated from the aquatic environment as determined by serotyping and amplification of the ribosomal DNA spacer regions.

Abstract:

:Salmonella species are pathogenic bacteria often detected in sewage, freshwater, marine coastal water, and groundwater. Salmonella spp. can survive for long periods in natural waters, and the persistence of specific and epidemic strains is of great concern in public health. However, the diversity of species found in the natural environment remains unknown. The aim of this study was to investigate the diversity of Salmonella strains isolated from different natural aquatic systems within a Mediterranean coastal watershed (river, wastewater, and marine coastal areas). A total of 574 strains isolated from these natural environments were identified by both conventional serotyping and the ribosomal spacer-heteroduplex polymorphism (RS-HP) method (M. A. Jensen and N. Straus, PCR Methods Appl. 3:186-194, 1993). More than 40 different serotypes were found, and some serotypes probably mobilized from widespread animal-rearing activities were detected only during storm events. These serotypes may be good indicators of specific contamination sources. Furthermore, the RS-HP method based on the PCR amplification of the intergenic spacer region between the 16S and 23S rRNA genes can produce amplicon profiles allowing the discrimination of species at both serotype and intraserotype levels. This method represents a powerful tool that could be used for rapid typing of Salmonella isolates.

journal_name

Appl Environ Microbiol

authors

Baudart J,Lemarchand K,Brisabois A,Lebaron P

doi

10.1128/aem.66.4.1544-1552.2000

keywords:

subject

Has Abstract

pub_date

2000-04-01 00:00:00

pages

1544-52

issue

4

eissn

0099-2240

issn

1098-5336

journal_volume

66

pub_type

杂志文章
  • Identification of unconventional intestinal pathogenic Escherichia coli isolates expressing intermediate virulence factor profiles by using a novel single-step multiplex PCR.

    abstract::Intestinal pathogenic Escherichia coli represents a global health problem for mammals, including humans. At present, diarrheagenic E. coli bacteria are grouped into seven major pathotypes that differ in their virulence factor profiles, severity of clinical manifestations, and prognosis. In this study, we developed and...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02855-06

    authors: Müller D,Greune L,Heusipp G,Karch H,Fruth A,Tschäpe H,Schmidt MA

    更新日期:2007-05-01 00:00:00

  • The gastrointestinal tract as a potential infection reservoir of digital dermatitis-associated treponemes in beef cattle and sheep.

    abstract::Digital dermatitis (DD) is an important cause of lameness in dairy cattle worldwide. It has now been reported in beef cattle and also sheep (contagious ovine digital dermatitis [CODD]). Three Treponema phylogroups are consistently isolated from lesions, Treponema medium-like, Treponema phagedenis-like, and Treponema p...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01956-15

    authors: Sullivan LE,Carter SD,Duncan JS,Grove-White DH,Angell JW,Evans NJ

    更新日期:2015-11-01 00:00:00

  • Se(VI) reduction and the precipitation of Se(0) by the facultative bacterium Enterobacter cloacae SLD1a-1 are regulated by FNR.

    abstract::The fate of selenium in the environment is controlled, in part, by microbial selenium oxyanion reduction and Se(0) precipitation. In this study, we identified a genetic regulator that controls selenate reductase activity in the Se-reducing bacterium Enterobacter cloacae SLD1a-1. Heterologous expression of the global a...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02542-06

    authors: Yee N,Ma J,Dalia A,Boonfueng T,Kobayashi DY

    更新日期:2007-03-01 00:00:00

  • Constitutive expression of the proteorhodopsin gene by a flavobacterium strain representative of the proteorhodopsin-producing microbial community in the North Sea.

    abstract::Proteorhodopsin (PR), a photoactive proton pump containing retinal, is present in approximately half of all bacteria in the ocean, but its physiological role is still unclear, since very few strains carrying the PR gene have been cultured. The aim of this work was to characterize PR diversity in a North Sea water samp...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02971-09

    authors: Riedel T,Tomasch J,Buchholz I,Jacobs J,Kollenberg M,Gerdts G,Wichels A,Brinkhoff T,Cypionka H,Wagner-Döbler I

    更新日期:2010-05-01 00:00:00

  • Screening and Evaluation of New Hydroxymethylfurfural Oxidases for Furandicarboxylic Acid Production.

    abstract::The enzymatic production of 2,5-furandicarboxylic acid (FDCA) from 5-hydroxymethylfurfural (HMF) has gained interest in recent years, as FDCA is a renewable precursor of poly(ethylene-2,5-furandicarboxylate) (PEF). 5-Hydroxymethylfurfural oxidases (HMFOs) form a flavoenzyme family with genes annotated in a dozen bacte...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00842-20

    authors: Viñambres M,Espada M,Martínez AT,Serrano A

    更新日期:2020-08-03 00:00:00

  • Links between plant and rhizoplane bacterial communities in grassland soils, characterized using molecular techniques.

    abstract::Molecular analysis of grassland rhizosphere soil has demonstrated complex and diverse bacterial communities, with resultant difficulties in detecting links between plant and bacterial communities. These studies have, however, analyzed "bulk" rhizosphere soil, rather than rhizoplane communities, which interact most clo...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.71.11.6784-6792.2005

    authors: Nunan N,Daniell TJ,Singh BK,Papert A,McNicol JW,Prosser JI

    更新日期:2005-11-01 00:00:00

  • Biochemical analyses of multiple endoxylanases from the rumen bacterium Ruminococcus albus 8 and their synergistic activities with accessory hemicellulose-degrading enzymes.

    abstract::Ruminococcus albus 8 is a ruminal bacterium capable of metabolizing hemicellulose and cellulose, the major components of the plant cell wall. The enzymes that allow this bacterium to capture energy from the two polysaccharides, therefore, have potential application in plant cell wall depolymerization, a process critic...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00353-11

    authors: Moon YH,Iakiviak M,Bauer S,Mackie RI,Cann IK

    更新日期:2011-08-01 00:00:00

  • Liposomes replace serum for cultivation of fermenting mycoplasmas.

    abstract::Cholesterol and albumin are limiting factors in the growth of Mycoplasma species. These nutrients are usually supplied in the culture medium by the addition of serum. The growth of M. pneumoniae in a serum-free medium containing an ethanolic cholesterol suspension and albumin was about one-half the level attained in s...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.46.2.370-374.1983

    authors: Cluss RG,Johnson JK,Somerson NL

    更新日期:1983-08-01 00:00:00

  • Genetic diversity and horizontal transfer of genes involved in oxidation of reduced phosphorus compounds by Alcaligenes faecalis WM2072.

    abstract::Enrichment was performed to isolate organisms that could utilize reduced phosphorus compounds as their sole phosphorus sources. One isolate that grew well with either hypophosphite or phosphite was identified by 16S rRNA gene analysis as a strain of Alcaligenes faecalis. The genes required for oxidation of hypophosphi...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.71.1.290-296.2005

    authors: Wilson MM,Metcalf WW

    更新日期:2005-01-01 00:00:00

  • Public health assessment of Salmonella enterica serovar enteritidis inactivated-vaccine treatment in layer flocks.

    abstract::Although there have been several reports on the efficacy assessment of a Salmonella enterica serovar Enteritidis vaccine against intestinal and parenchymatous organ diseases of laying hens, no public health risk characterization of its long-term effect on eggs has been reported. In this study, we attempted to assess t...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01689-08

    authors: Toyota-Hanatani Y,Ekawa T,Ohta H,Igimi S,Hara-Kudo Y,Sasai K,Baba E

    更新日期:2009-02-01 00:00:00

  • Isolation of amoebae from edible mushrooms.

    abstract::Isolation of amoebae from the surface and internal tissues of edible mushrooms was investigated. Samples tested over a 3-year period included mushrooms cultivated from six geographic localities. Of 168 mushroom surfaces tested, 161 (96%) yielded amoebae. Of 166 samples of internal stalk and cap tissues tested, only 1 ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.44.1.255-257.1982

    authors: Napolitano JJ

    更新日期:1982-07-01 00:00:00

  • Genomic island TnSmu2 of Streptococcus mutans harbors a nonribosomal peptide synthetase-polyketide synthase gene cluster responsible for the biosynthesis of pigments involved in oxygen and H2O2 tolerance.

    abstract::The oral biofilm community consists of >800 microbial species, among which Streptococcus mutans is considered a primary pathogen for dental caries. The genomic island TnSmu2 of S. mutans comprises >2% of the genome. In this study, we demonstrate that TnSmu2 harbors a gene cluster encoding nonribosomal peptide syntheta...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.03079-09

    authors: Wu C,Cichewicz R,Li Y,Liu J,Roe B,Ferretti J,Merritt J,Qi F

    更新日期:2010-09-01 00:00:00

  • Rapid detection of members of the family Enterobacteriaceae by a monoclonal antibody.

    abstract::Six monoclonal antibodies directed against enterobacteria were produced and characterized. The specificity of one of these antibodies (CX9/15; immunoglobulin G2a) was studied by indirect immunofluorescence against 259 enterobacterial strains and 125 other gram-negative bacteria. All of the enterobacteria were specific...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.58.5.1524-1529.1992

    authors: Levasseur S,Husson MO,Leitz R,Merlin F,Laurent F,Peladan F,Drocourt JL,Leclerc H,Van Hoegaerden M

    更新日期:1992-05-01 00:00:00

  • Wolbachia Endosymbionts Modify Drosophila Ovary Protein Levels in a Context-Dependent Manner.

    abstract:UNLABELLED:Endosymbiosis is a unique form of interaction between organisms, with one organism dwelling inside the other. One of the most widespread endosymbionts is Wolbachia pipientis, a maternally transmitted bacterium carried by insects, crustaceans, mites, and filarial nematodes. Although candidate proteins that co...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01255-16

    authors: Christensen S,Pérez Dulzaides R,Hedrick VE,Momtaz AJ,Nakayasu ES,Paul LN,Serbus LR

    更新日期:2016-08-15 00:00:00

  • Quantitative Determination of the Spatial Distribution of Nitrosomonas europaea and Nitrobacter agilis Cells Immobilized in kappa-Carrageenan Gel Beads by a Specific Fluorescent-Antibody Labelling Technique.

    abstract::A novel technique, combining labelling and stereological methods, for the determination of spatial distribution of two microorganisms in a biofilm is presented. Cells of Nitrosomonas europaea (ATCC 19718) and Nitrobacter agilis (ATCC 14123) were homogeneously distributed in a kappa-carrageenan gel during immobilizatio...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.59.6.1951-1954.1993

    authors: Hunik JH,van den Hoogen MP,de Boer W,Smit M,Tramper J

    更新日期:1993-06-01 00:00:00

  • Characterization of the complete zwittermicin A biosynthesis gene cluster from Bacillus cereus.

    abstract::Bacillus cereus UW85 produces the linear aminopolyol antibiotic zwittermicin A (ZmA). This antibiotic has diverse biological activities, such as suppression of disease in plants caused by protists, inhibition of fungal and bacterial growth, and amplification of the insecticidal activity of the toxin protein from Bacil...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02518-08

    authors: Kevany BM,Rasko DA,Thomas MG

    更新日期:2009-02-01 00:00:00

  • Two-dimensional electrophoresis study of Lactobacillus delbrueckii subsp. bulgaricus thermotolerance.

    abstract::The response of Lactobacillus delbrueckii subsp. bulgaricus cells to heat stress was studied by use of a chemically defined medium. Two-dimensional electrophoresis (2-DE) analysis was used to correlate the kinetics of heat shock protein (HSP) induction with cell recovery from heat injury. We demonstrated that enhanced...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.68.3.1055-1063.2002

    authors: Gouesbet G,Jan G,Boyaval P

    更新日期:2002-03-01 00:00:00

  • Chlortetracycline-resistant intestinal bacteria in organically raised and feral Swine.

    abstract::Organically raised swine had high fecal populations of chlortetracycline (CTC)-resistant (growing at 64 μg CTC/ml) Escherichia coli, Megasphaera elsdenii, and anaerobic bacteria. By comparison, CTC-resistant bacteria in feral swine feces were over 1,000-fold fewer and exhibited lower taxonomic diversity. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00688-11

    authors: Stanton TB,Humphrey SB,Stoffregen WC

    更新日期:2011-10-01 00:00:00

  • Multiphasic approach to study the bacterial ecology of fermented sausages inoculated with a commercial starter culture.

    abstract::In this paper, the ability of a commercial starter culture to perform a sausage fermentation is evaluated. Molecular analysis revealed the presence of several strains of the same species contained in the starter culture with different behavior during the fermentation, and the contribution of Lactobacillus curvatus, wh...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.72.1.942-945.2006

    authors: Cocolin L,Urso R,Rantsiou K,Cantoni C,Comi G

    更新日期:2006-01-01 00:00:00

  • Demethylation of dimethylsulfoniopropionate to 3-mercaptopropionate by an aerobic marine bacterium.

    abstract::A bacterium, strain BIS-6, that grew aerobically on dimethylsulfoniopropionate (DMSP) was isolated from an intertidal mud sample. Strain BIS-6 quantitatively demethylated DMSP and 3-methiolpropionate to 3-mercaptopropionate. Strain BIS-6 was a versatile methylotroph growing on the osmolytes DMSP and glycine betaine an...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.60.12.4617-4619.1994

    authors: Visscher PT,Taylor BF

    更新日期:1994-12-01 00:00:00

  • Construction and expression of a bifunctional single-chain antibody against Bacillus cereus p6ores.

    abstract::The variable-region genes of monoclonal antibody against Bacillus cereus spores were cloned from mouse hybridoma cells by reverse transcription-PCR. The heavy- and light-chain variable-region genes were connected by a 45-base linker DNA to allow folding of the fusion protein into a functional tertiary structure. For d...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.64.7.2490-2496.1998

    authors: Koo K,Foegeding PM,Swaisgood HE

    更新日期:1998-07-01 00:00:00

  • Cyclodextrins as carriers of cholesterol and fatty acids in cultivation of mycoplasmas.

    abstract::The design of fully or partly defined media for mycoplasma cultivation involves the need to provide the essential lipids, cholesterol and long-chain fatty acids, in an assimilable and nontoxic form. This study introduces cyclodextrins (CDs) as carriers of these lipids, thus suggesting alternatives to serum or bovine s...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.59.2.547-551.1993

    authors: Greenberg-Ofrath N,Terespolosky Y,Kahane I,Bar R

    更新日期:1993-02-01 00:00:00

  • Fluorescence correlation spectroscopy to study diffusion and reaction of bacteriophages inside biofilms.

    abstract::In the natural environment, most of the phages that target bacteria are thought to exist in biofilm ecosystems. The purpose of this study was to gain a clearer understanding of the reactivity of these viral particles when they come into contact with bacteria embedded in biofilms. Experimentally, we quantified lactococ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02304-07

    authors: Briandet R,Lacroix-Gueu P,Renault M,Lecart S,Meylheuc T,Bidnenko E,Steenkeste K,Bellon-Fontaine MN,Fontaine-Aupart MP

    更新日期:2008-04-01 00:00:00

  • Development of an amylolytic Lactobacillus plantarum silage strain expressing the Lactobacillus amylovorus alpha-amylase gene.

    abstract::An amylolytic Lactobacillus plantarum silage strain with the starch-degrading ability displayed by Lactobacillus amylovorus was developed. An active fragment of the gene coding for alpha-amylase production in L. amylovorus was cloned and integrated into the chromosome of the competitive inoculant strain L. plantarum L...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.60.10.3529-3535.1994

    authors: Fitzsimons A,Hols P,Jore J,Leer RJ,O'Connell M,Delcour J

    更新日期:1994-10-01 00:00:00

  • Coliform aerosols generated from the surface of dewatered sewage applied to a forest clearcut.

    abstract::Concentrations of airborne coliform bacteria as high as 1.5 X 10(4) m-3 were observed 8 cm above anaerobically digested sewage sludge applied to a forest clearcut. Dry conditions and high wind speeds tended to favor aerosol generation. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.36.6.972-974.1978

    authors: Edmonds RL,Littke W

    更新日期:1978-12-01 00:00:00

  • Sorption and metabolism of metolachlor by a bacterial community.

    abstract::A stable bacterial community absorbed and transformed the herbicide metolachlor [2-chloro-N-(2-ethyl- 6-methylphenyl)-N-(2-methoxy-1-methylethyl)-acetamide] from a liquid medium. About 80% of the added ring-[U-C]metolachlor (50 mug/ml) disappeared from the medium and accumulated inside the cells. The ratio of cellular...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.55.3.733-740.1989

    authors: Liu SY,Zheng Z,Zhang R,Bollag JM

    更新日期:1989-03-01 00:00:00

  • Size-selective predation on groundwater bacteria by nanoflagellates in an organic-contaminated aquifer.

    abstract::Time series incubations were conducted to provide estimates for the size selectivities and rates of protistan grazing that may be occurring in a sandy, contaminated aquifer. The experiments involved four size classes of fluorescently labeled groundwater bacteria (FLB) and 2- to 3-microns-long nanoflagellates, primaril...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.64.2.618-625.1998

    authors: Kinner NE,Harvey RW,Blakeslee K,Novarino G,Meeker LD

    更新日期:1998-02-01 00:00:00

  • Prokaryotic metabolic activity and community structure in Antarctic continental shelf sediments.

    abstract::The prokaryote community activity and structural characteristics within marine sediment sampled across a continental shelf area located off eastern Antarctica (66 degrees S, 143 degrees E; depth range, 709 to 964 m) were studied. Correlations were found between microbial biomass and aminopeptidase and chitinase rates,...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.69.5.2448-2462.2003

    authors: Bowman JP,McCammon SA,Gibson JA,Robertson L,Nichols PD

    更新日期:2003-05-01 00:00:00

  • Characterization of a Nuclear Polyhedrosis Virus Isolated from Diseased Gonometa podocarpi (Lepidoptera:Lasiocampidae).

    abstract::Gonometa podocarpi is an important pest of several species of pine in East Africa, and large numbers of trees in plantations in Kenya were partially or completely defoliated by the larval stage of this insect. After the infestation in the Mt. Elgon region, large numbers of dead and moribund larvae were found on the gr...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.42.2.308-316.1981

    authors: Moore NF,Armstrong T,Green B,Brown D,Hibbin J,Kelly DC,Tinsley TW,Aloo TC

    更新日期:1981-08-01 00:00:00

  • Oxidation of ethane by an Acremonium species.

    abstract::Ethane oxidation was studied in ethane-grown resting cells (mycelia) of an Acremonium sp. and in cell-free preparations of such mycelia. From resting cell experiments evidence was found for a pathway of ethane oxidation via ethanol, acetaldehyde, and acetic acid. In vitro studies indicated that ethane-oxidizing activi...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.32.1.14-20.1976

    authors: Davies JS,Wellman AM,Zajic JE

    更新日期:1976-07-01 00:00:00