Restoration of growth phenotypes of Escherichia coli DH5alpha in minimal media through reversal of a point mutation in purB.

Abstract:

:A point mutation (E115K) resulting in slower growth of Escherichia coli DH5alpha and XL1-Blue in minimal media was identified in the purB gene, coding for adenylosuccinate lyase (ASL), through complementation with an E. coli K-12 genomic library and serial subcultures. Chromosomal modification reversing the mutation to the wild type restored growth phenotypes in minimal media.

journal_name

Appl Environ Microbiol

authors

Jung SC,Smith CL,Lee KS,Hong ME,Kweon DH,Stephanopoulos G,Jin YS

doi

10.1128/AEM.01210-10

subject

Has Abstract

pub_date

2010-09-01 00:00:00

pages

6307-9

issue

18

eissn

0099-2240

issn

1098-5336

pii

AEM.01210-10

journal_volume

76

pub_type

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