Microplate assay for colletotrichum spore production.

abstract：:Because dihydrostreptomycin can remain as a slowly removed antibiotic residue in dairy animals and because of the need for more sensitive procedures with which to provide information concerning antibiotic residues in food products, procedures were developed in more sensitive assays of dihydrostreptomycin in milk and s...

journal_title：Applied and environmental microbiology

authors： Inglis JM,Katz SE

更新日期：1978-03-01 00:00:00

abstract：:An L-glutamine-overproducing mutant of an Escherichia coli K-12-derived strain was selected from randomly mutagenized cells in the course of L-alanyl-L-glutamine strain development. Genome-wide mutation analysis unveiled a novel mechanism for L-glutamine overproduction in this mutant. Three mutations were identified t...

journal_title：Applied and environmental microbiology

authors： Hayashi M,Tabata K

更新日期：2013-05-01 00:00:00

abstract：:The effects of long-term heavy metal deposition on microbial community structure and the level of bacterial community tolerance were studied along two different gradients in Scandinavian coniferous forest soils. One was near the Harjavalta smelter in Finland, and one was at Ronnskar in Sweden. Phospholipid fatty acid ...

journal_title：Applied and environmental microbiology

authors： Pennanen T,Frostegard A,Fritze H,Baath E

更新日期：1996-02-01 00:00:00

abstract：:Pathogens may reach agricultural soils through application of animal manure and thereby pose a risk of contaminating crops as well as surface and groundwater. Treatment and handling of manure for improved nutrient and odor management may also influence the amount and fate of manure-borne pathogens in the soil. A study...

journal_title：Applied and environmental microbiology

authors： Amin MG,Forslund A,Bui XT,Juhler RK,Petersen SO,Lægdsmand M

更新日期：2013-01-01 00:00:00

abstract：:Lysostaphin represents a promising therapeutic agent for the treatment of staphylococcal infections, in particular those of methicillin-resistant Staphylococcus aureus (MRSA). However, conventional expression systems for the enzyme suffer from various limitations, and there remains a need for an efficient and cost-eff...

journal_title：Applied and environmental microbiology

authors： Zhao H,Blazanovic K,Choi Y,Bailey-Kellogg C,Griswold KE

更新日期：2014-05-01 00:00:00

abstract：UNLABELLED:High-throughput sequencing (HTS) is revolutionizing environmental surveys of microbial diversity in the three domains of life by providing detailed information on which taxa are present in microbial assemblages. However, it is still unclear how the relative abundance of specific taxa gathered by HTS correlat...

journal_title：Applied and environmental microbiology

authors： Giner CR,Forn I,Romac S,Logares R,de Vargas C,Massana R

更新日期：2016-07-15 00:00:00

abstract：:We have isolated and characterized the cDNA and genomic DNA coding for a phenoloxidase, laccase I, previously purified from culture supernatant of the newly isolated ligninolytic basidiomycete PM1 (CECT 2971). A cDNA library from basidiomycete PM1 was constructed, and laccase-encoding cDNAs were identified by screenin...

journal_title：Applied and environmental microbiology

authors： Coll PM,Tabernero C,Santamaría R,Pérez P

更新日期：1993-12-01 00:00:00

abstract：:We describe the development and validation of a method for the qualitative analysis of complex bifidobacterial communities based on PCR and denaturing gradient gel electrophoresis (DGGE). Bifidobacterium genus-specific primers were used to amplify an approximately 520-bp fragment from the 16S ribosomal DNA (rDNA), and...

journal_title：Applied and environmental microbiology

authors： Satokari RM,Vaughan EE,Akkermans AD,Saarela M,de Vos WM

更新日期：2001-02-01 00:00:00

abstract：:Epifluorescent microscopy and flow cytometry were used in different combinations with fluorescein isothiocyanate-labeled immunoglobulins M and G3 to estimate the numbers of Cryptosporidium parvum oocysts in soil extracts containing 10 to 10,017 oocysts/ml. No combination had a systematic effect on accuracy or precisio...

journal_title：Applied and environmental microbiology

authors： Walker M,Redelman D

更新日期：2004-03-01 00:00:00

abstract：:Heavy metal resistance by bacteria is a topic of much importance to the bioremediation of contaminated soils and sediments. We report here the isolation of a highly cadmium-resistant Klebsiella planticola strain, Cd-1, from reducing salt marsh sediments. The strain grows in up to 15 mM CdCl(2) under a wide range of Na...

journal_title：Applied and environmental microbiology

authors： Sharma PK,Balkwill DL,Frenkel A,Vairavamurthy MA

更新日期：2000-07-01 00:00:00

abstract：:Actinomycete isolates from indoor air and dust in water-damaged schools and children's day care centers were tested for toxicity by using boar spermatozoa as an indicator. Toxicity was detected in extracts of four strains which caused a loss of sperm motility, and the 50% effective concentrations (EC50) were 10 to 63 ...

journal_title：Applied and environmental microbiology

authors： Andersson MA,Mikkola R,Kroppenstedt RM,Rainey FA,Peltola J,Helin J,Sivonen K,Salkinoja-Salonen MS

更新日期：1998-12-01 00:00:00

abstract：:Antibody production against aflatoxin B1 was compared in three rabbits and one goat. Titers obtained were 20 times higher in the rabbits than in the goat. The goat antiserum appeared to have a higher degree of cross-reactivity for other aflatoxins and related metabolites than did the rabbit antiserum. ...

journal_title：Applied and environmental microbiology

authors： Gaur PK,El-Nakib O,Chu FS

更新日期：1980-09-01 00:00:00

abstract：:Petuely's selective medium for Bifidobacterium was improved by addition of riboflavin, nucleic acid bases, pyruvic acid, and nalidixic acid. The modified medium, when examined under strictly anaerobic conditions for efficient isolation of Bifidobacterium from human fecal samples, exhibited selective and high viable co...

journal_title：Applied and environmental microbiology

authors： Tanaka R,Mutai M

更新日期：1980-11-01 00:00:00

abstract：:Lactobacillus species normally found in the intestinal tract of humans varied in the ability to deconjugate bile acids, whereas laboratory strains of Lactobacillus acidophilus deconjugated both glycocholate and taurocholate. All isolates of L. acidophilus from human feces deconjugated taurocholate, whereas only one of...

journal_title：Applied and environmental microbiology

authors： Gilliland SE,Speck ML

更新日期：1977-01-01 00:00:00

abstract：:Solid media on a base of B-12 or CB medium with agarose or agarose of low melting temperature were developed for the cultivation of Microcystis species. The media with 0.4% gel showed the highest number of CFU, and increasing the gel concentration resulted in a reduction of the number of CFU. There was no difference i...

journal_title：Applied and environmental microbiology

authors： Shirai M,Matumaru K,Ohotake A,Takamura Y,Aida T,Nakano M

更新日期：1989-10-01 00:00:00

abstract：:Aldehydes are a class of chemicals with many industrial uses. Several aldehydes are responsible for flavors and fragrances present in plants, but aldehydes are not known to accumulate in most natural microorganisms. In many cases, microbial production of aldehydes presents an attractive alternative to extraction from ...

journal_title：Applied and environmental microbiology

authors： Kunjapur AM,Prather KL

更新日期：2015-03-01 00:00:00

abstract：:A human feeding study was performed with Lactococcus lactis TC165.5, which is genetically marked by insertion of the sucrose-nisin conjugative transposon Tn5276 and chromosomal resistance to rifampin and streptomycin. The fate of strain TC165.5 and its nucleic acids was monitored by conventional plating methods and by...

journal_title：Applied and environmental microbiology

authors： Klijn N,Weerkamp AH,de Vos WM

更新日期：1995-07-01 00:00:00

abstract：:Trench leachate samples collected anoxically from shallow-land, low-level radioactive waste disposal sites were analyzed for total aerobic and anaerobic populations, sulfate reducers, denitrifiers, and methanogens. Among the several aerobic and anaerobic bacteria isolated, only Bacillus sp., Pseudomonas sp., Citrobact...

journal_title：Applied and environmental microbiology

authors： Francis AJ,Dobbs S,Nine BJ

更新日期：1980-07-01 00:00:00

abstract：:Toluene dioxygenase from Pseudomonas putida F1 has been implicated as an enzyme capable of degrading trichloroethylene. This has now been confirmed with Escherichia coli JM109(pDTG601) that contains the structural genes (todC1C2BA) of toluene dioxygenase under the control of the tac promoter. The extent of trichloroet...

journal_title：Applied and environmental microbiology

authors： Zylstra GJ,Wackett LP,Gibson DT

更新日期：1989-12-01 00:00:00

abstract：:Vibrio alginolyticus is one of the most abundant microorganisms in marine environments and is also an opportunistic pathogen mediating high-mortality vibriosis in marine animals. Alternative sigma factors play essential roles in bacterial pathogens in the adaptation to environmental changes during infection and the ad...

journal_title：Applied and environmental microbiology

authors： Gu D,Zhang J,Hao Y,Xu R,Zhang Y,Ma Y,Wang Q

更新日期：2019-07-01 00:00:00

abstract：:Our previous genetic analysis (J. W. Willis, J. K. Engwall, and A. K. Chatterjee, Phytopathology 77:1199-1205, 1987) had revealed a tight linkage between pel-3 (pel, pectate lyase gene) and peh-1 (peh, polygalacturonase gene) within the chromosome of Erwinia carotovora subsp. carotovora 71. Nucleotide sequencing, tran...

journal_title：Applied and environmental microbiology

authors： Liu Y,Chatterjee A,Chatterjee AK

更新日期：1994-07-01 00:00:00

abstract：UNLABELLED:The l-isoaspartyl protein carboxyl methyltransferase (PCM) repairs protein damage resulting from spontaneous conversion of aspartyl or asparaginyl residues to isoaspartate and increases long-term stationary-phase survival of Escherichia coli under stress. In the course of studies intended to examine PCM func...

journal_title：Applied and environmental microbiology

authors： VandenBerg KE,Ahn S,Visick JE

更新日期：2016-08-15 00:00:00

abstract：:Two 16S rRNA-targeted oligonucleotide probes were designed for the archaeal kingdoms Euryachaeota and Crenarchaeota. Probe specificities were evaluated by nonradioactive dot blot hybridization against selected reference organisms. The successful application of fluorescent-probe derivatives for whole-cell hybridization...

journal_title：Applied and environmental microbiology

authors： Burggraf S,Mayer T,Amann R,Schadhauser S,Woese CR,Stetter KO

更新日期：1994-09-01 00:00:00

abstract：:The genetic diversity of 35 Bacillus sphaericus strains was analyzed by a newly developed multilocus sequence typing (MLST) scheme, toxin gene pool survey, and mosquito bioassay. The results demonstrated that strains assigned to the same sequence type (ST) had the same occurrence of toxin genes. Further sequence analy...

journal_title：Applied and environmental microbiology

authors： Ge Y,Hu X,Zheng D,Wu Y,Yuan Z

更新日期：2011-08-01 00:00:00

abstract：:Lipid accumulation and fatty acid composition in Candida 107 have been studied using a two-stage continuous culture system in which the first vessel was run under carbon-limited conditions and then the entire output was passed into a second vessel, where lipid accumulation was stimulated by adding only glucose. Maximu...

journal_title：Applied and environmental microbiology

authors： Hall MJ,Ratledge C

更新日期：1977-03-01 00:00:00

abstract：:Parental strains and asporogenous mutants of Bacillus thuringiensis subspp. kurstaki and aizawai produced high yields of delta-endotoxin on M medium, which contained 330 mug of potassium per ml, but not on ST and ST-a media, each of which contained only 11 mug of potassium per ml. On ST and ST-a media, refractile gran...

journal_title：Applied and environmental microbiology

authors： Wakisaka Y,Masaki E,Nishimoto Y

更新日期：1982-06-01 00:00:00

abstract：:Microscopic eukaryotes are known to have important ecosystem functions, but their diversity in most environments remains vastly unexplored. Here we analyzed an 18S rRNA gene library from a subsurface iron- and sulfur-oxidizing microbial community growing in highly acidic (pH < 0.9) runoff within the Richmond Mine at I...

journal_title：Applied and environmental microbiology

authors： Baker BJ,Tyson GW,Goosherst L,Banfield JF

更新日期：2009-04-01 00:00:00

abstract：:Bacterial communities associated with marine algae are often dominated by members of the Roseobacter clade, and in the present study, we describe Roseobacter phenotypes that may provide this group of bacteria with selective advantages when colonizing this niche. Nine of 14 members of the Roseobacter clade, of which ha...

journal_title：Applied and environmental microbiology

authors： Bruhn JB,Gram L,Belas R

更新日期：2007-01-01 00:00:00

abstract：:An agar medium containing 5% cholesterol has been developed for the isolation, enumeration, and characterization of cholesterol-reducing strains of Eubacterium. ...

journal_title：Applied and environmental microbiology

authors： Brinkley AW,Gottesman AR,Mott GE

更新日期：1980-12-01 00:00:00

abstract：:Current methods for detection of Cryptosporidium parvum oocysts in water are time-consuming and difficult. We have developed a reverse transcription (RT)-PCR which can detect the presence of a single viable oocyst spiked into concentrated environmental water samples. The test is based on the detection of mRNA from a C...

journal_title：Applied and environmental microbiology

authors： Stinear T,Matusan A,Hines K,Sandery M

更新日期：1996-09-01 00:00:00