Microbial activity of trench leachates from shallow-land, low-level radioactive waste disposal sites.

abstract：:Tropomyosin receptor kinase A (TrkA), a receptor tyrosine kinase, is known to be associated with various diseases. Thus, TrkA has become a major drug-screening target for these diseases. Despite the fact that the production of recombinant proteins by prokaryotic hosts has advantages, such as fast growth and ease of ge...

journal_title：Applied and environmental microbiology

authors： Honda T,Yasuda T,Tanaka T,Hagiwara K,Arai T,Yoshino T

更新日期：2015-02-01 00:00:00

abstract：:Acetoin reductase (ACR) catalyzes the conversion of acetoin to 2,3-butanediol. Under certain conditions, Clostridium acetobutylicum ATCC 824 (and strains derived from it) generates both d- and l-stereoisomers of acetoin, but because of the absence of an ACR enzyme, it does not produce 2,3-butanediol. A gene encoding A...

journal_title：Applied and environmental microbiology

authors： Siemerink MA,Kuit W,López Contreras AM,Eggink G,van der Oost J,Kengen SW

更新日期：2011-04-01 00:00:00

abstract：:Maximum yields of amylase were produced by the thermophilic actinomycete Thermomonospora viridis in a modified Simpson and McCoy medium containing 1.5% corn starch and 0.5% mycological peptone with an initial pH 7.0. Best yields of amylase were obtained after incubation for 48 h, when the pH of the medium had risen to...

journal_title：Applied and environmental microbiology

authors： Upton ME,Fogarty WM

更新日期：1977-01-01 00:00:00

abstract：:A sensitive and specific method for detection of Listeria monocytogenes in milk and ground-beef samples is described. It consists of culturing samples in listeria enrichment broth (LEB) and subculturing them from LEB to listeria plating media, followed by DNA extraction and species-specific detection of the organism b...

journal_title：Applied and environmental microbiology

authors： Thomas EJ,King RK,Burchak J,Gannon VP

更新日期：1991-09-01 00:00:00

abstract：:We recently described a new method to activate antibiotic production in bacteria by introducing a mutation conferring resistance to a drug such as streptomycin, rifampin, paromomycin, or gentamicin. This method, however, enhanced antibiotic production by only up to an order of magnitude. Working with Streptomyces coel...

journal_title：Applied and environmental microbiology

authors： Wang G,Hosaka T,Ochi K

更新日期：2008-05-01 00:00:00

abstract：:The interaction between Glomus intraradices and Pratylenchus coffeae on transformed carrot roots was studied in root organ culture. G. intraradices provided the roots with increased protection against P. coffeae by suppressing nematode reproduction in the roots. The internal and external mycorrhizal development was no...

journal_title：Applied and environmental microbiology

authors： Elsen A,Declerck S,De Waele D

更新日期：2003-07-01 00:00:00

abstract：:Methane is an undesirable end product of rumen fermentative activity because of associated environmental impacts and reduced host feed efficiency. Our study characterized the rumen microbial methanogenic community in beef cattle divergently selected for phenotypic residual feed intake (RFI) while offered a high-forage...

journal_title：Applied and environmental microbiology

authors： Carberry CA,Waters SM,Kenny DA,Creevey CJ

更新日期：2014-01-01 00:00:00

abstract：:The polyene macrolide antibiotic nystatin produced by Streptomyces noursei contains a deoxyaminosugar mycosamine moiety attached to the C-19 carbon of the macrolactone ring through the beta-glycosidic bond. The nystatin biosynthetic gene cluster contains three genes, nysDI, nysDII, and nysDIII, encoding enzymes with p...

journal_title：Applied and environmental microbiology

authors： Nedal A,Sletta H,Brautaset T,Borgos SE,Sekurova ON,Ellingsen TE,Zotchev SB

更新日期：2007-11-01 00:00:00

abstract：:The subunit composition of the extracellular complex from Clostridium thermocellum was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Twenty-six bands, representing proteins with apparent molecular sizes ranging from 37,500 to 185,000 Da, could be detected by silver staining. Cultiva...

journal_title：Applied and environmental microbiology

authors： Kohring S,Wiegel J,Mayer F

更新日期：1990-12-01 00:00:00

abstract：UNLABELLED:The ability to sense and adapt to temperature fluctuation is critical to the aquatic survival, transmission, and infectivity of Vibrio cholerae, the causative agent of the disease cholera. Little information is available on the physiological changes that occur when V. cholerae experiences temperature shifts....

journal_title：Applied and environmental microbiology

authors： Townsley L,Sison Mangus MP,Mehic S,Yildiz FH

更新日期：2016-06-30 00:00:00

abstract：:A gene, designated cry11B, encoding a 81,293-Da crystal protein of Bacillus thuringiensis subsp. jegathesan was cloned by using a gene-specific oligonucleotide probe. The sequence of the Cry11B protein, as deduced from the sequence of the cry11B gene, contains large regions of similarity with the Cry11A toxin (previou...

journal_title：Applied and environmental microbiology

authors： Delécluse A,Rosso ML,Ragni A

更新日期：1995-12-01 00:00:00

abstract：:Deletion of the pcaHG genes, encoding protocatechuate 3,4-dioxygenase in Rhodococcus jostii RHA1, gives a gene deletion strain still able to grow on protocatechuic acid as the sole carbon source, indicating a second degradation pathway for protocatechuic acid. Metabolite analysis of wild-type R. jostii RHA1 grown on m...

journal_title：Applied and environmental microbiology

authors： Spence EM,Scott HT,Dumond L,Calvo-Bado L,di Monaco S,Williamson JJ,Persinoti GF,Squina FM,Bugg TDH

更新日期：2020-09-17 00:00:00

abstract：:Enzymatic activities that could be involved in methanethiol generation in five cheese-ripening bacteria were assayed, and the major sulfur compounds produced were identified. L-Methionine and alpha-keto-gamma-methyl-thio-butyric acid demethiolating activities were detected in whole cells and cell extracts (CFEs) of al...

journal_title：Applied and environmental microbiology

authors： Bonnarme P,Psoni L,Spinnler HE

更新日期：2000-12-01 00:00:00

abstract：:Decisions as to the disposition of swine carcasses with lesions attributable to a mycobacterial infection are based upon a lesion criterion which is used as a diagnostic test by federal meat inspectors. Using this criterion, a federal meat inspector classified 58 of 100 pigs as "passed for cooking" and the other 42 pi...

journal_title：Applied and environmental microbiology

authors： Brown J,Neuman MA

更新日期：1979-04-01 00:00:00

abstract：:We analyzed changes in bacterioplankton morphology and composition during enhanced protozoan grazing by image analysis and fluorescent in situ hybridization with group-specific rRNA-targeted oligonucleotide probes. Enclosure experiments were conducted in a small, fishless freshwater pond which was dominated by the cla...

journal_title：Applied and environmental microbiology

authors： Jürgens K,Pernthaler J,Schalla S,Amann R

更新日期：1999-03-01 00:00:00

abstract：:Tn5 insertion mutants of a soil isolate, Pseudomonas fluorescens Pf0-1, were selected for decreased ability to adhere to quartz sand in a column assay. Three adhesion-deficient mutants that differed in the location of the Tn5 insertion in the chromosome were isolated and compared with the wild-type strain. One mutant,...

journal_title：Applied and environmental microbiology

authors： Deflaun MF,Marshall BM,Kulle EP,Levy SB

更新日期：1994-07-01 00:00:00

abstract：:The cellulosomes produced by Clostridium cellulovorans are organized by the specific interactions between the cohesins in the scaffolding proteins and the dockerins of the catalytic components. Using a cohesin biomarker, we identified a cellulosomal enzyme which belongs to the glycosyl hydrolase family 5 and has a dom...

journal_title：Applied and environmental microbiology

authors： Jeon SD,Lee JE,Kim SJ,Park SH,Choi GW,Han SO

更新日期：2013-10-01 00:00:00

abstract：:Hepatitis A virus (HAV) is a food-borne enteric virus responsible for outbreaks of hepatitis associated with shellfish consumption. The objectives of this study were to determine the thermal inactivation behavior of HAV in blue mussels, to compare the first-order and Weibull models to describe the data, to calculate A...

journal_title：Applied and environmental microbiology

authors： Bozkurt H,D'Souza DH,Davidson PM

更新日期：2014-05-01 00:00:00

abstract：:The lytic activity of 132 phages isolated during slow acid production in cheese factories situated in all the dairying regions of France during the past 16 years has been determined on 291 strains of mesophilic lactic streptococci. The results have been treated according to a method of analysis of data so as to establ...

journal_title：Applied and environmental microbiology

authors： Chopin MC,Chopin A,Roux C

更新日期：1976-12-01 00:00:00

abstract：:The growth characteristics and acetate production of several Escherichia coli strains were compared by using shake flasks, batch fermentations, and glucose-feedback-controlled fed-batch fermentations to assess the potential of each strain to grow at high cell densities. Of the E. coli strains tested, including JM105, ...

journal_title：Applied and environmental microbiology

authors： Luli GW,Strohl WR

更新日期：1990-04-01 00:00:00

abstract：:16S rRNA gene-targeted group-specific primers were designed and validated for specific detection and quantification of the Clostridium leptum subgroup and the Atopobium cluster. To monitor the predominant bacteria in human feces by real-time PCR, we used these specific primers together with four sets of group-specific...

journal_title：Applied and environmental microbiology

authors： Matsuki T,Watanabe K,Fujimoto J,Takada T,Tanaka R

更新日期：2004-12-01 00:00:00

abstract：:Aluminum, long known to be detrimental to soybean productivity, was localized in the polyphosphate granules (PPG) of bacteroids in root nodules of soybean plants. By using energy-dispersive X-ray analysis, bacteroids in early infections were shown to have typical PPG constituents. However, in PPG in older infections a...

journal_title：Applied and environmental microbiology

authors： Roth LE,Dunlap JR,Stacey G

更新日期：1987-10-01 00:00:00

abstract：:We have cloned the homoserine dehydrogenase genes (hom) from the gram-negative obligate methylotrophs Methylobacillus glycogenes ATCC 21276 and ATCC 21371 by complementation of an Escherichia coli homoserine dehydrogenase-deficient mutant. The 4.15-kb DNA fragment cloned from M. glycogenes ATCC 21371 also complemented...

journal_title：Applied and environmental microbiology

authors： Motoyama H,Maki K,Anazawa H,Ishino S,Teshiba S

更新日期：1994-01-01 00:00:00

abstract：:An organism capable of growth on pyridine was isolated from soil by enrichment culture techniques and identified as Micrococcus luteus. The organism oxidized pyridine for energy and released N contained in the pyridine ring as ammonium. The organism could not grow on mono- or disubstituted pyridinecarboxylic acids or ...

journal_title：Applied and environmental microbiology

authors： Sims GK,Sommers LE,Konopka A

更新日期：1986-05-01 00:00:00

abstract：:Lake Fryxell is a perennially ice-covered lake in the McMurdo Dry Valleys, Antarctica, with a sharp oxycline in a water column that is density stabilized by a gradient in salt concentration. Dissolved oxygen falls from 20 mg liter(-1) to undetectable over one vertical meter from 8.9- to 9.9-m depth. We provide the fir...

journal_title：Applied and environmental microbiology

authors： Jungblut AD,Hawes I,Mackey TJ,Krusor M,Doran PT,Sumner DY,Eisen JA,Hillman C,Goroncy AK

更新日期：2015-11-13 00:00:00

abstract：:Type III protein secretion systems play a key role in the virulence of many pathogenic proteobacteria, but they also occur in nonpathogenic, plant-associated bacteria. Certain type III protein secretion genes (e.g., hrcC) have been found in Pseudomonas sp. strain SBW25 (and other biocontrol pseudomonads), but other ty...

journal_title：Applied and environmental microbiology

authors： Rezzonico F,Défago G,Moënne-Loccoz Y

更新日期：2004-09-01 00:00:00

abstract：:The objective of the present study was to evaluate the anti-inflammatory effects of Bifidobacterium animalis subsp. lactis strain BB12 in stimulated Caco-2 cells and to characterize the factors responsible for these anti-inflammatory effects. Characterization and purification studies indicate that BB12's anti-inflamma...

journal_title：Applied and environmental microbiology

authors： Wang Z,Wang J,Cheng Y,Liu X,Huang Y

更新日期：2011-11-01 00:00:00

abstract：:Although the high level of competence for natural transformation of Acinetobacter sp. strain BD413 has been the subject of numerous studies, only two competence genes, comC and comP, have been identified to date. By chromosomal walking analysis we found two overlapping open reading frames, designated comE and comF, st...

journal_title：Applied and environmental microbiology

authors： Busch S,Rosenplänter C,Averhoff B

更新日期：1999-10-01 00:00:00

abstract：:Five Pseudomonas aeruginosa strains were tested for the utilization of 47 low-molecular-weight compounds as their sole sources of carbon and energy for growth at a concentration of 2.5 g/liter. Of these compounds, 31 to 35 were consumed. Growth experiments in tap water at 15 degrees C were carried out with one particu...

journal_title：Applied and environmental microbiology

authors： van der Kooij D,Oranje JP,Hijnen WA

更新日期：1982-11-01 00:00:00

abstract：:It has long been assumed that prolonged holding of environmental samples at the ambient air temperature prior to bacteriological analysis is detrimental to isolation and detection of Vibrio cholerae, the causative agent of pandemic cholera. The present study was aimed at understanding the effect of transporting enviro...

journal_title：Applied and environmental microbiology

authors： Alam M,Sadique A,Nur-A-Hasan,Bhuiyan NA,Nair GB,Siddique AK,Sack DA,Ahsan S,Huq A,Sack RB,Colwell RR

更新日期：2006-03-01 00:00:00