Cloning and expression of a novel toxin gene from Bacillus thuringiensis subsp. jegathesan encoding a highly mosquitocidal protein.

abstract：:The plasmid-free strain Lactococcus lactis subsp. cremoris BC101 produced an extracellular proteinase physicochemically similar to the proteinase encoded by the plasmid-linked prtP gene of other lactococcal strains. The absence of detectable plasmids in strain BC101 indicated that the prtP proteinase gene may be chrom...

journal_title：Applied and environmental microbiology

authors： Nissen-Meyer J,Lillehaug D,Nes IF

更新日期：1992-02-01 00:00:00

abstract：:Previously, we successfully cloned a d-cycloserine (d-CS) biosynthetic gene cluster consisting of 10 open reading frames (designated dcsA to dcsJ) from d-CS-producing Streptomyces lavendulae ATCC 11924. In this study, we put four d-CS biosynthetic genes (dcsC, dcsD, dcsE, and dcsG) in tandem under the control of the T...

journal_title：Applied and environmental microbiology

authors： Kumagai T,Ozawa T,Tanimoto M,Noda M,Matoba Y,Sugiyama M

更新日期：2015-11-01 00:00:00

abstract：:An assay has been developed to detect bacterial glycosidases in colonies grown on the surface of agar plates. Advantages of this technique over previously described methods include elimination of the need for replica plating, better visualization of chromagenic reaction products, and a simple permeabilization step to ...

journal_title：Applied and environmental microbiology

authors： Paoni NF,Arroyo RL

更新日期：1984-01-01 00:00:00

abstract：:Aerobic anoxygenic phototrophic (AAP) bacteria are photoheterotrophic microbes that are found in a broad range of aquatic environments. Although potentially significant to the microbial ecology and biogeochemistry of marine ecosystems, their abundance and genetic diversity and the environmental variables that regulate...

journal_title：Applied and environmental microbiology

authors： Ritchie AE,Johnson ZI

更新日期：2012-04-01 00:00:00

abstract：:Fusarium verticillioides (Gibberella fujikuroi mating population A [MP-A]) is a widespread pathogen on maize and is well-known for producing fumonisins, mycotoxins that cause severe disease in animals and humans. The species is a member of the Gibberella fujikuroi species complex, which consists of at least 11 differe...

journal_title：Applied and environmental microbiology

authors： Bömke C,Rojas MC,Hedden P,Tudzynski B

更新日期：2008-12-01 00:00:00

abstract：:Methanogenic isolates did not consume hydrogen below partial pressures of 6.5 Pa. Thus, in contrast to a previous report, results from pure-culture studies do not invalidate the threshold model for methane production from hydrogen in sediments. ...

journal_title：Applied and environmental microbiology

authors： Lovley DR

更新日期：1985-06-01 00:00:00

abstract：:Vegetative mycelia and spores of the investigated high- and low-producer strains of Streptomyces griseus bound significant amounts (4%) of streptomycin, which could be removed by increasing ionic strength. The release of antibiotic from the spores was easier when the spores were germinating. This phenomenon is conside...

journal_title：Applied and environmental microbiology

authors： Szabó I,Benedek A,Barabás G

更新日期：1985-08-01 00:00:00

abstract：:A method was developed to study the biodegradation and oxidative biodehalogenation of fluorinated phenols by 19F nuclear magnetic resonance (NMR). Characterization of the 19F NMR spectra of metabolite profiles of a series of fluorophenols, converted by purified phenol hydroxylase, catechol 1,2-dioxygenase, and/or by t...

journal_title：Applied and environmental microbiology

authors： Boersma MG,Dinarieva TY,Middelhoven WJ,van Berkel WJ,Doran J,Vervoort J,Rietjens IM

更新日期：1998-04-01 00:00:00

abstract：:Vinyl chloride reductases (VC-RDase) are the key enzymes for complete microbial reductive dehalogenation of chloroethenes, including the groundwater pollutants tetrachloroethene and trichloroethene. Analysis of the codon usage of the VC-RDase genes vcrA and bvcA showed that these genes are highly unusual and are chara...

journal_title：Applied and environmental microbiology

authors： McMurdie PJ,Behrens SF,Holmes S,Spormann AM

更新日期：2007-04-01 00:00:00

abstract：:[This corrects the article on p. 469 in vol. 57.]. ...

journal_title：Applied and environmental microbiology

authors：

更新日期：1991-05-01 00:00:00

abstract：:A single gene (xylB) encoding both beta-D-xylosidase (EC 3.2.1.37) and alpha-L-arabinofuranosidase (EC 3.2.1.55) activities was identified and sequenced from the ruminal bacterium Butyrivibrio fibrisolvens. The xylB gene consists of a 1.551-bp open reading frame (ORF) encoding 517 amino acids. A subclone containing a ...

journal_title：Applied and environmental microbiology

authors： Utt EA,Eddy CK,Keshav KF,Ingram LO

更新日期：1991-04-01 00:00:00

abstract：:The bioconversion of sugars present in wood hemicellulose to 2,3-butanediol by Klebsiella pneumoniae grown on high sugar concentrations was investigated. When K. pneumoniae was grown under finite air conditions in the presence of added acetic acid, 50 g of D-glucose and D-xylose per liter could be converted to 25 and ...

journal_title：Applied and environmental microbiology

authors： Yu EK,Saddler JN

更新日期：1983-09-01 00:00:00

abstract：:In Pseudomonas aeruginosa, the catabolite repression control (Crc) protein repressed the formation of the blue pigment pyocyanin in response to a preferred carbon source (succinate) by interacting with phzM mRNA, which encodes a key enzyme in pyocyanin biosynthesis. Crc bound to an extended imperfect recognition seque...

journal_title：Applied and environmental microbiology

authors： Huang J,Sonnleitner E,Ren B,Xu Y,Haas D

更新日期：2012-07-01 00:00:00

abstract：:Zoothamnium niveum is a giant, colonial marine ciliate from sulfide-rich habitats obligatorily covered with chemoautotrophic, sulfide-oxidizing bacteria which appear as coccoid rods and rods with a series of intermediate shapes. Comparative 16S rRNA gene sequence analysis and fluorescence in situ hybridization showed ...

journal_title：Applied and environmental microbiology

authors： Rinke C,Schmitz-Esser S,Stoecker K,Nussbaumer AD,Molnár DA,Vanura K,Wagner M,Horn M,Ott JA,Bright M

更新日期：2006-03-01 00:00:00

abstract：:Samples of a sandy loam soil taken from a long-term liming experiment in southeast England were amended with solutions of metal sulfate salts. Soils with a range of pHs were amended to contain Cu, Cd, or Zn at concentrations around the maximum permissible values for these metals in agricultural land receiving sewage s...

journal_title：Applied and environmental microbiology

authors： Knight BP,McGrath SP,Chaudri AM

更新日期：1997-01-01 00:00:00

abstract：:Anaerobic acetate degradation at 70 degrees C and at 55 degrees C (as a reference) was studied by running laboratory upflow anaerobic sludge blanket (UASB) reactors inoculated with mesophilic granular sludge. In UASB reactors fed with acetate-containing media (3 g of chemical oxygen demand [COD] per liter, correspondi...

journal_title：Applied and environmental microbiology

authors： Rintala J,Lepistö S,Ahring B

更新日期：1993-06-01 00:00:00

abstract：:Knowledge of the mechanism of pressure-induced inactivation of microorganisms could be helpful in defining an effective, relatively mild pressure treatment as a means of decontamination, especially in combination with other physical treatments or antimicrobial agents. We have studied the effect of high pressure on Lac...

journal_title：Applied and environmental microbiology

authors： Wouters PC,Glaasker E,Smelt JP

更新日期：1998-02-01 00:00:00

abstract：:We investigated the growth parameters of Saccharomyces rouxii isolated from spoiled chocolate syrup. The optimum pH range for S. rouxii was 3.5 to 5.5, whereas the minimum and maximum pH values that permitted growth were 1.5 and 10.5, respectively. For cells grown in 0 and 60% sucrose the optimum water activity (aw) v...

journal_title：Applied and environmental microbiology

authors： Restaino L,Bills S,Tscherneff K,Lenovich LM

更新日期：1983-05-01 00:00:00

abstract：:Citrate metabolism by Enterococcus faecium FAIR-E 198, an isolate from Greek Feta cheese, was studied in modified MRS (mMRS) medium under different pH conditions and glucose and citrate concentrations. In the absence of glucose, this strain was able to metabolize citrate in a pH range from constant pH 5.0 to 7.0. At a...

journal_title：Applied and environmental microbiology

authors： Vaningelgem F,Ghijsels V,Tsakalidou E,De Vuyst L

更新日期：2006-01-01 00:00:00

abstract：:Pseudomonas putida KT2440, a saprophytic soil bacterium that colonizes the plant root, is a suitable microorganism for the removal of pollutants and a stable host for foreign genes used in biotransformation processes. Because of its potential use in agriculture and industry, we investigated the conditions for the opti...

journal_title：Applied and environmental microbiology

authors： Muñoz-Rojas J,Bernal P,Duque E,Godoy P,Segura A,Ramos JL

更新日期：2006-01-01 00:00:00

abstract：:Streptococcus mutans 6715-15 and Streptococcus sanguis 10558 were grown together in continuous culture with glucose as the limiting carbon source. The relationship of growth rate to substrate concentration was determined for pure cultures of each organism in continuous and batch cultures. A model based on competition ...

journal_title：Applied and environmental microbiology

authors： Kemp CW,Robrish SA,Curtis MA,Sharer SA,Bowen WH

更新日期：1983-04-01 00:00:00

abstract：:We investigated the relationship between bacterioplankton production (BP), respiration (BR), and community composition measured by terminal restriction fragment length polymorphism in the southern North Sea over a seasonal cycle. Major changes in bacterioplankton richness were apparent from April to December. While ce...

journal_title：Applied and environmental microbiology

authors： Reinthaler T,Winter C,Herndl GJ

更新日期：2005-05-01 00:00:00

abstract：:Environmental bacteria are exposed to a myriad of biotic interactions that influence their function and survival. The grazing activity of protozoan predators significantly impacts the dynamics, diversification, and evolution of bacterial communities in soil ecosystems. To evade protozoan predation, bacteria employ var...

journal_title：Applied and environmental microbiology

authors： Mazzola M,de Bruijn I,Cohen MF,Raaijmakers JM

更新日期：2009-11-01 00:00:00

abstract：:The following two factors significantly influence estimates of the maximum specific growth rate ( micro (max)) and the lag-phase duration (lambda): (i) the technique used to monitor bacterial growth and (ii) the model fitted to estimate parameters. In this study, nine strains of Listeria monocytogenes were monitored s...

journal_title：Applied and environmental microbiology

authors： Baty F,Flandrois JP,Delignette-Muller ML

更新日期：2002-12-01 00:00:00

abstract：:Resistance to desiccation was utilized in the selection of highly radiation-resistant asporogenous bacteria from non-irradiated sources. A bacterial suspension in phosphate buffer was dried in a thin film at 25 degrees C and 33% relative humidity. Storage under these conditions for 15 days or more reduced the number o...

journal_title：Applied and environmental microbiology

authors： Sanders SW,Maxcy RB

更新日期：1979-09-01 00:00:00

abstract：UNLABELLED:Endosymbiosis is a unique form of interaction between organisms, with one organism dwelling inside the other. One of the most widespread endosymbionts is Wolbachia pipientis, a maternally transmitted bacterium carried by insects, crustaceans, mites, and filarial nematodes. Although candidate proteins that co...

journal_title：Applied and environmental microbiology

authors： Christensen S,Pérez Dulzaides R,Hedrick VE,Momtaz AJ,Nakayasu ES,Paul LN,Serbus LR

更新日期：2016-08-15 00:00:00

abstract：:Epilithic periphyton communities were sampled at three sites on the Minnesota shoreline of Lake Superior from June 2004 to August 2005 to determine if fecal coliforms and Escherichia coli were present throughout the ice-free season. Fecal coliform densities increased up to 4 orders of magnitude in early summer, reache...

journal_title：Applied and environmental microbiology

authors： Ksoll WB,Ishii S,Sadowsky MJ,Hicks RE

更新日期：2007-06-01 00:00:00

abstract：:A new strategy for the detection of infectious Cryptosporidium parvum oocysts in water samples, which combines immunomagnetic separation (IMS) for recovery of oocysts with in vitro cell culturing and PCR (CC-PCR), was field tested with a total of 122 raw source water samples and 121 filter backwash water grab samples ...

journal_title：Applied and environmental microbiology

authors： Di Giovanni GD,Hashemi FH,Shaw NJ,Abrams FA,LeChevallier MW,Abbaszadegan M

更新日期：1999-08-01 00:00:00

abstract：:Trichoderma reesei can produce up to 100 g/liter of extracellular proteins. The major and industrially relevant products are cellobiohydrolase I (CBHI) and the hemicellulase XYNI. The genes encoding both enzymes are transcriptionally activated by the regulatory protein Xyr1. The first 850 nucleotides of the cbh1 promo...

journal_title：Applied and environmental microbiology

authors： Kiesenhofer DP,Mach RL,Mach-Aigner AR

更新日期：2017-12-15 00:00:00

abstract：:The food-borne pathogen Vibrio parahaemolyticus has been reported as being present in New Zealand (NZ) seawaters, but there have been no reported outbreaks of food-borne infection from commercially grown NZ seafood. Our study determined the current incidence of V. parahaemolyticus in NZ oysters and Greenshell mussels ...

journal_title：Applied and environmental microbiology

authors： Cruz CD,Hedderley D,Fletcher GC

更新日期：2015-04-01 00:00:00