Trichloroethylene degradation by Escherichia coli containing the cloned Pseudomonas putida F1 toluene dioxygenase genes.

Abstract:

:Toluene dioxygenase from Pseudomonas putida F1 has been implicated as an enzyme capable of degrading trichloroethylene. This has now been confirmed with Escherichia coli JM109(pDTG601) that contains the structural genes (todC1C2BA) of toluene dioxygenase under the control of the tac promoter. The extent of trichloroethylene degradation by the recombinant organism depended on the cell concentration and the concentration of trichloroethylene. A linear rate of trichloroethylene degradation was observed with the E. coli recombinant strain. In contrast, P. putida F39/D, a mutant strain of P. putida F1 that does not contain cis-toluene dihydrodiol dehydrogenase, showed a much faster initial rate of trichloroethylene degradation which decreased over time.

journal_name

Appl Environ Microbiol

authors

Zylstra GJ,Wackett LP,Gibson DT

doi

10.1128/AEM.55.12.3162-3166.1989

subject

Has Abstract

pub_date

1989-12-01 00:00:00

pages

3162-6

issue

12

eissn

0099-2240

issn

1098-5336

journal_volume

55

pub_type

杂志文章
  • Fluorescence in situ hybridization and spectral imaging of coral-associated bacterial communities.

    abstract::Microbial communities play important roles in the functioning of coral reef communities. However, extensive autofluorescence of coral tissues and endosymbionts limits the application of standard fluorescence in situ hybridization (FISH) techniques for the identification of the coral-associated bacterial communities. T...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.72.4.3016-3020.2006

    authors: Ainsworth TD,Fine M,Blackall LL,Hoegh-Guldberg O

    更新日期:2006-04-01 00:00:00

  • Investigation of genotypes of Borrelia burgdorferi in Ixodes scapularis ticks collected during surveillance in Canada.

    abstract::The genetic diversity of Borrelia burgdorferi sensu stricto, the agent of Lyme disease in North America, has consequences for the performance of serological diagnostic tests and disease severity. To investigate B. burgdorferi diversity in Canada, where Lyme disease is emerging, bacterial DNA in 309 infected adult Ixod...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02636-10

    authors: Ogden NH,Margos G,Aanensen DM,Drebot MA,Feil EJ,Hanincová K,Schwartz I,Tyler S,Lindsay LR

    更新日期:2011-05-01 00:00:00

  • Enhancement of varicella-zoster virus replication in cultured human embryonic lung cells treated with the pesticide carbaryl.

    abstract::In studies designed to determine the factors responsible for control of herpesvirus replicaton in an infected cell, we examined the interaction of varicella-zoster (VZ) virus-infected human embryonic lung cells with the pesticide carbaryl. The replication of the cell-associated VZ virus was enhanced 2- to 13-fold as c...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.41.3.652-656.1981

    authors: Abrahamsen LH,Jerkofsky M

    更新日期:1981-03-01 00:00:00

  • Identification and characterization of a novel flagellum-dependent Salmonella-infecting bacteriophage, iEPS5.

    abstract::A novel flagellatropic phage of Salmonella enterica serovar Typhimurium, called iEPS5, was isolated and characterized. iEPS5 has an icosahedral head and a long noncontractile tail with a tail fiber. Genome sequencing revealed a double-stranded DNA of 59,254 bp having 73 open reading frames (ORFs). To identify the rece...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00706-13

    authors: Choi Y,Shin H,Lee JH,Ryu S

    更新日期:2013-08-01 00:00:00

  • Purification and characterization of a pyruvated-mannose-specific xanthan lyase from heat-stable, salt-tolerant bacteria.

    abstract::A xanthanase complex secreted by a consortium of heat-stable, salt-tolerant bacteria includes a lyase that specifically removes terminal pyruvated beta-d-mannose residues from the side chains of xanthan gum. The enzyme was purified to homogeneity from the culture broth following ion-exchange chromatography and gel per...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.57.9.2523-2528.1991

    authors: Ahlgren JA

    更新日期:1991-09-01 00:00:00

  • Direct assessment of viral diversity in soils by random PCR amplification of polymorphic DNA.

    abstract::Viruses are the most abundant and diverse biological entities within soils, yet their ecological impact is largely unknown. Defining how soil viral communities change with perturbation or across environments will contribute to understanding the larger ecological significance of soil viruses. A new approach to examinin...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00268-13

    authors: Srinivasiah S,Lovett J,Polson S,Bhavsar J,Ghosh D,Roy K,Fuhrmann JJ,Radosevich M,Wommack KE

    更新日期:2013-09-01 00:00:00

  • Diverse and unique picocyanobacteria in Chesapeake Bay, revealed by 16S-23S rRNA internal transcribed spacer sequences.

    abstract::rRNA internal transcribed spacer phylogeny showed that Chesapeake Bay is populated with diverse Synechococcus strains, including members of the poorly studied marine cluster B. Marine cluster B prevailed in the upper bay, while marine cluster A was common in the lower bay. Interestingly, marine cluster B Synechococcus...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.72.3.2239-2243.2006

    authors: Chen F,Wang K,Kan J,Suzuki MT,Wommack KE

    更新日期:2006-03-01 00:00:00

  • Excystation and culturing of human and animal Giardia spp. by using gerbils and TYI-S-33 medium.

    abstract::Mongolian gerbils were used as an animal model to excyst and host Giardia spp. isolated from meadow voles, dogs, beavers, and humans. Both cysts and trophozoites were used to establish infections. Gerbils were infected with Giardia duodenalis from beaver, dog, and human sources, and the trophozoites were extracted and...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.51.3.647-651.1986

    authors: Wallis PM,Wallis HM

    更新日期:1986-03-01 00:00:00

  • Ice Nucleation Activity in Fusarium acuminatum and Fusarium avenaceum.

    abstract::Twenty fungal genera, including 14 Fusarium species, were examined for ice nucleation activity at -5.0 degrees C, and this activity was found only in Fusarium acuminatum and Fusarium avenaceum. This characteristic is unique to these two species. Ice nucleation activity of F. avenaceum was compared with ice nucleation ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.58.9.2960-2964.1992

    authors: Pouleur S,Richard C,Martin JG,Antoun H

    更新日期:1992-09-01 00:00:00

  • Identification of bacterial populations in dairy wastewaters by use of 16S rRNA gene sequences and other genetic markers.

    abstract::Hydraulic flush waste removal systems coupled to solid/liquid separators and circulated treatment lagoons are commonly utilized to manage the large amounts of animal waste produced on high-intensity dairy farms. Although these systems are common, little is known about the microbial populations that inhabit them or how...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.70.7.4267-4275.2004

    authors: McGarvey JA,Miller WG,Sanchez S,Stanker L

    更新日期:2004-07-01 00:00:00

  • Pathway of glucose catabolism by strain VeGlc2, an anaerobe belonging to the verrucomicrobiales lineage of bacterial descent

    abstract::Strain VeGlc2, an anaerobic ultramicrobacterium belonging to the Verrucomicrobiales lineage of bacterial descent, fermented glucose to acetate, propionate, succinate, and CO2. The distribution of radiolabel in the fermentation end products produced from position-labelled glucose and in vitro measurements of enzyme act...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.64.12.4830-4833.1998

    authors: Janssen PH

    更新日期:1998-12-01 00:00:00

  • Detection of Coxiella burnetii in complex matrices by using multiplex quantitative PCR during a major Q fever outbreak in The Netherlands.

    abstract::Q fever, caused by Coxiella burnetii, is a zoonosis with a worldwide distribution. A large rural area in the southeast of the Netherlands was heavily affected by Q fever between 2007 and 2009. This initiated the development of a robust and internally controlled multiplex quantitative PCR (qPCR) assay for the detection...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.05097-11

    authors: de Bruin A,de Groot A,de Heer L,Bok J,Wielinga PR,Hamans M,van Rotterdam BJ,Janse I

    更新日期:2011-09-01 00:00:00

  • Purification and Comparison of the Periplasmic and Extracellular Forms of the Cellodextrinase from Bacteroides succinogenes.

    abstract::Both the periplasmic and the extracellular cellodextrinases from Bacteroides succinogenes S85 grown on Avicel microcrystalline cellulose were purified to homogeneity by column chromatography and characterized. Over 70% of the total cellobiosidase activity displayed by cells was accounted for by these enzymes. The peri...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.54.6.1488-1493.1988

    authors: Huang L,Forsberg CW

    更新日期:1988-06-01 00:00:00

  • Selection of clc, cba, and fcb chlorobenzoate-catabolic genotypes from groundwater and surface waters adjacent to the Hyde park, Niagara Falls, chemical landfill.

    abstract::The frequency of isolation of three nonhomologous chlorobenzoate catabolic genotypes (clc, cba, and fcb) was determined for 464 isolates from freshwater sediments and groundwater in the vicinity of the Hyde Park industrial landfill site in the Niagara watershed. Samples were collected from both contaminated and noncon...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.65.4.1627-1635.1999

    authors: Peel MC,Wyndham RC

    更新日期:1999-04-01 00:00:00

  • Enhancing the production of hydroxyl radicals by Pleurotus eryngii via quinone redox cycling for pollutant removal.

    abstract::The induction of hydroxyl radical (OH) production via quinone redox cycling in white-rot fungi was investigated to improve pollutant degradation. In particular, we examined the influence of 4-methoxybenzaldehyde (anisaldehyde), Mn(2+), and oxalate on Pleurotus eryngii OH generation. Our standard quinone redox cycling ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02138-08

    authors: Gómez-Toribio V,García-Martín AB,Martínez MJ,Martínez AT,Guillén F

    更新日期:2009-06-01 00:00:00

  • Response of Sesbania grandiflora to Inoculation of Soil with Vesicular-Arbuscular Mycorrhizal Fungi.

    abstract::A greenhouse experiment was conducted to determine the influence of two tropical isolates of Glomus fasciculatum and Glomus mosseae on the nutrient uptake and growth of Sesbania grandiflora. Inoculation of sterile soil with the fungi significantly improved growth and nutrient uptake by S. grandiflora, but the response...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.50.3.701-703.1985

    authors: Habte M,Aziz T

    更新日期:1985-09-01 00:00:00

  • Engineered Escherichia coli silver-binding periplasmic protein that promotes silver tolerance.

    abstract::Silver toxicity is a problem that microorganisms face in medical and environmental settings. Through exposure to silver compounds, some bacteria have adapted to growth in high concentrations of silver ions. Such adapted microbes may be dangerous as pathogens but, alternatively, could be potentially useful in nanomater...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.06823-11

    authors: Sedlak RH,Hnilova M,Grosh C,Fong H,Baneyx F,Schwartz D,Sarikaya M,Tamerler C,Traxler B

    更新日期:2012-04-01 00:00:00

  • Bacterial adherence to polystyrene: a replica method of screening for bacterial hydrophobicity.

    abstract::A simple replica method is described for the rapid identification of colonies of bacteria which adhere to polystyrene. A correlation was found between the adherence of bacterial strains to polystyrene and cell surface hydrophobicity, suggesting the use of this technique in screening for cell surface mutants and in the...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.42.2.375-377.1981

    authors: Rosenberg M

    更新日期:1981-08-01 00:00:00

  • Effects of pH of the medium on flagellation of Vibrio parahaemolyticus.

    abstract::Formation of the lateral flagella by Vibrio parahaemolyticus was inhibited under an alkaline condition of the medium. However, flagellation of the polar monotrichous flagellum was not affected in the same condition. Flagellation of the lateral flagella depended on the pH of the medium. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.37.6.1248-1249.1979

    authors: Kimura K,Tateiri S,Iida H

    更新日期:1979-06-01 00:00:00

  • Detection of low levels of Listeria monocytogenes cells by using a fiber-optic immunosensor.

    abstract::Biosensor technology has a great potential to meet the need for sensitive and nearly real-time microbial detection from foods. An antibody-based fiber-optic biosensor to detect low levels of Listeria monocytogenes cells following an enrichment step was developed. The principle of the sensor is a sandwich immunoassay w...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.70.10.6138-6146.2004

    authors: Geng T,Morgan MT,Bhunia AK

    更新日期:2004-10-01 00:00:00

  • Nonclinical and clinical Enterococcus faecium strains, but not Enterococcus faecalis strains, have distinct structural and functional genomic features.

    abstract::Certain strains of Enterococcus faecium and Enterococcus faecalis contribute beneficially to animal health and food production, while others are associated with nosocomial infections. To determine whether there are structural and functional genomic features that are distinct between nonclinical (NC) and clinical (CL) ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.03108-13

    authors: Kim EB,Marco ML

    更新日期:2014-01-01 00:00:00

  • GlcNAc De-N-Acetylase from the Hyperthermophilic Archaeon Sulfolobus solfataricus.

    abstract::Sulfolobus solfataricus is an aerobic crenarchaeal hyperthermophile with optimum growth at temperatures greater than 80°C and pH 2 to 4. Within the crenarchaeal group of Sulfolobales, N-acetylglucosamine (GlcNAc) has been shown to be a component of exopolysaccharides, forming their biofilms, and of the N-glycan decora...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01879-18

    authors: Iacono R,Strazzulli A,Maurelli L,Curci N,Casillo A,Corsaro MM,Moracci M,Cobucci-Ponzano B

    更新日期:2019-01-09 00:00:00

  • Characterization of the complete zwittermicin A biosynthesis gene cluster from Bacillus cereus.

    abstract::Bacillus cereus UW85 produces the linear aminopolyol antibiotic zwittermicin A (ZmA). This antibiotic has diverse biological activities, such as suppression of disease in plants caused by protists, inhibition of fungal and bacterial growth, and amplification of the insecticidal activity of the toxin protein from Bacil...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02518-08

    authors: Kevany BM,Rasko DA,Thomas MG

    更新日期:2009-02-01 00:00:00

  • Mycotoxic porcine nephropathy and spontaneous occurrence of ochratoxin A residues in kidneys and blood of Polish swine.

    abstract::Kidneys showing renal changes characteristic for mycotoxic porcine nephropathy were collected during the period 1 April 1982 to 31 March 1983 from 225,000 swine processed in a large slaughterhouse in the district of Poznań, Poland. Of 113 kidneys suspected of mycotoxic porcine nephropathy, 27 exhibited ochratoxin A le...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.47.6.1210-1212.1984

    authors: Goliński P,Hult K,Grabarkiewicz-Szczesna J,Chełkowski J,Kneblewski P,Szebiotko K

    更新日期:1984-06-01 00:00:00

  • Enumeration of polysaccharide-degrading Bacteroides species in human feces by using species-specific DNA probes.

    abstract::DNA probes that are specific for each of five predominant species of human colonic Bacteroides (B. thetaiotaomicron, B. uniformis, B. distasonis, "Bacteroides group 3452-A", and B. ovatus) were used to detect and enumerate these species in fecal samples from two adult volunteers. These five species are capable of ferm...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.51.2.385-390.1986

    authors: Kuritza AP,Shaughnessy P,Salyers AA

    更新日期:1986-02-01 00:00:00

  • The Legionella pneumophila collagen-like protein mediates sedimentation, autoaggregation, and pathogen-phagocyte interactions.

    abstract::Although only partially understood, multicellular behavior is relatively common in bacterial pathogens. Bacterial aggregates can resist various host defenses and colonize their environment more efficiently than planktonic cells. For the waterborne pathogen Legionella pneumophila, little is known about the roles of aut...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.03254-13

    authors: Abdel-Nour M,Duncan C,Prashar A,Rao C,Ginevra C,Jarraud S,Low DE,Ensminger AW,Terebiznik MR,Guyard C

    更新日期:2014-02-01 00:00:00

  • Osmoregulation in symbiosis-independent mutants of Bdellovibrio bacteriovorus.

    abstract::Bdellovibrios capable of axenic growth grow in a cell-free medium at a rate considerably lower than that attainable in a two-membered culture with Escherichia coli. The axenic growth rate may be improved either by adjustment of the osmosity of the medium or by the addition of low concentrations of spermine. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.33.5.1207-1208.1977

    authors: Varon M,Seijffers J

    更新日期:1977-05-01 00:00:00

  • Differential sunlight sensitivity of picophytoplankton from surface Mediterranean Coastal Waters.

    abstract::We tested the sensitivity of coastal picophytoplankton exposed to natural sunlight in short-term experiments. Cell abundance and cell-specific chlorophyll fluorescence were significantly reduced in Prochlorococcus spp. but not in Synechococcus, whereas picoeukaryotes had an intermediate response. These results are the...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.71.4.2154-2157.2005

    authors: Sommaruga R,Hofer JS,Alonso-Sáez L,Gasol JM

    更新日期:2005-04-01 00:00:00

  • Development and characterization of lactose-positive pediococcus species for milk fermentation.

    abstract::Bacteriophages against Streptococcus thermophilus are a growing problem in the Italian cheese industry. One possible control method involves replacing S. thermophilus in mozzarella starter blends with lactic acid bacteria from a different genus or species. In this study, we evaluated lactose-positive pediococci for th...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.62.3.936-941.1996

    authors: Caldwell SL,McMahon DJ,Oberg CJ,Broadbent JR

    更新日期:1996-03-01 00:00:00

  • Dynamic Dispersal of Surface Layer Biofilm Induced by Nanosized TiO2 Based on Surface Plasmon Resonance and Waveguide.

    abstract::Pollutant degradation is present mainly in the surface layer of biofilms, and the surface layer is the most vulnerable to impairment by toxic pollutants. In this work, the effects of nanosized TiO2 (n-TiO2) on the average thicknesses of Bacillus subtilis biofilm and on bacterial attachment on different surfaces were i...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00047-18

    authors: Zhang P,Guo JS,Yan P,Chen YP,Wang W,Dai YZ,Fang F,Wang GX,Shen Y

    更新日期:2018-04-16 00:00:00