Abstract:
:Toluene dioxygenase from Pseudomonas putida F1 has been implicated as an enzyme capable of degrading trichloroethylene. This has now been confirmed with Escherichia coli JM109(pDTG601) that contains the structural genes (todC1C2BA) of toluene dioxygenase under the control of the tac promoter. The extent of trichloroethylene degradation by the recombinant organism depended on the cell concentration and the concentration of trichloroethylene. A linear rate of trichloroethylene degradation was observed with the E. coli recombinant strain. In contrast, P. putida F39/D, a mutant strain of P. putida F1 that does not contain cis-toluene dihydrodiol dehydrogenase, showed a much faster initial rate of trichloroethylene degradation which decreased over time.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Zylstra GJ,Wackett LP,Gibson DTdoi
10.1128/AEM.55.12.3162-3166.1989subject
Has Abstractpub_date
1989-12-01 00:00:00pages
3162-6issue
12eissn
0099-2240issn
1098-5336journal_volume
55pub_type
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