Detection of Cryptosporidium parvum in soil extracts.

Abstract:

:Epifluorescent microscopy and flow cytometry were used in different combinations with fluorescein isothiocyanate-labeled immunoglobulins M and G3 to estimate the numbers of Cryptosporidium parvum oocysts in soil extracts containing 10 to 10,017 oocysts/ml. No combination had a systematic effect on accuracy or precision. Background debris may have produced overestimates at low oocyst concentrations when flow cytometry was used.

journal_name

Appl Environ Microbiol

authors

Walker M,Redelman D

doi

10.1128/aem.70.3.1827-1829.2004

keywords:

subject

Has Abstract

pub_date

2004-03-01 00:00:00

pages

1827-9

issue

3

eissn

0099-2240

issn

1098-5336

journal_volume

70

pub_type

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