Abstract:
:The role of polyethylene glycol (PEG) in the transformation of Schizosaccharomyces pombe by electroporation is investigated by fluorescein isothiocyanate-dextran uptake and transformation studies. It is shown that when S. pombe cells are electroporated in the presence of PEG, the permeability state created is sustained until removal of PEG. In addition, the permeability of electroporated S. pombe envelopes is further increased with longer incubation times in PEG. The increased permeability is apparently a result of enlarged pores (electropores) due to the presence of PEG. Comparison of a heat pulse transformation protocol with electroporation suggests a second role for PEG in the uptake of macromolecules. Since pores are not thought to be created during a heat pulse, the PEG may be facilitating the uptake of plasmid DNA. This facilitation of uptake would also be expected to affect DNA uptake by electroporated cells.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Hood MT,Stachow Cdoi
10.1128/AEM.58.4.1201-1206.1992keywords:
subject
Has Abstractpub_date
1992-04-01 00:00:00pages
1201-6issue
4eissn
0099-2240issn
1098-5336journal_volume
58pub_type
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