Influence of Polyethylene Glycol on the Size of Schizosaccharomyces pombe Electropores.

Abstract:

:The role of polyethylene glycol (PEG) in the transformation of Schizosaccharomyces pombe by electroporation is investigated by fluorescein isothiocyanate-dextran uptake and transformation studies. It is shown that when S. pombe cells are electroporated in the presence of PEG, the permeability state created is sustained until removal of PEG. In addition, the permeability of electroporated S. pombe envelopes is further increased with longer incubation times in PEG. The increased permeability is apparently a result of enlarged pores (electropores) due to the presence of PEG. Comparison of a heat pulse transformation protocol with electroporation suggests a second role for PEG in the uptake of macromolecules. Since pores are not thought to be created during a heat pulse, the PEG may be facilitating the uptake of plasmid DNA. This facilitation of uptake would also be expected to affect DNA uptake by electroporated cells.

journal_name

Appl Environ Microbiol

authors

Hood MT,Stachow C

doi

10.1128/AEM.58.4.1201-1206.1992

keywords:

subject

Has Abstract

pub_date

1992-04-01 00:00:00

pages

1201-6

issue

4

eissn

0099-2240

issn

1098-5336

journal_volume

58

pub_type

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