Effect of visible light on progressive dormancy of Escherichia coli cells during the survival process in natural fresh water.


:Some effects of visible light on the survival of Escherichia coli in waters of the Butrón river were studied by comparing illuminated and nonilluminated systems. The following count methods were used: CFU on a selective medium (eosin-methylene blue agar), CFU on a medium of recuperation (Trypticase soy agar with yeast extract and glucose), number of metabolically active cells by reduction of 2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyl tetrazolium chloride (INT) to INT-formazan, and total number of E. coli cells as determined by the acridine orange direct-count method. In the illuminated systems, decreases in CFU of E. coli and in the number of metabolically active cells were observed. However, no decline of the total number of E. coli cells was observed. By count methods, different stages of progressive dormancy of E. coli cells were determined to exist in illuminated systems. Culturable and recoverable cells were defined as viable cells, and metabolically active cells and morphologically intact cells were defined as somnicells. Indirect activity measurements were also done by using [14C]glucose. In illuminated systems, a decrease of glucose uptake by E. coli cells was observed throughout the experiments. The assimilated fraction of [14C]glucose decreased faster than the respired fraction in illuminated systems. The percentage of respired [14C]glucose (14CO2 production) with respect to the total glucose uptake increased throughout the experiments, and the percentage of assimilated glucose decreased. Therefore, the visible light was also responsible for an additional inhibition of biosynthetic processes.


Appl Environ Microbiol


Barcina I,González JM,Iriberri J,Egea L




Has Abstract


1989-01-01 00:00:00












  • Association of Campylobacter jejuni with laying hens and eggs.

    abstract::Laying hens were individually caged at 20 weeks of age and tested for fecal excretion of Campylobacter jejuni (minimum level of detection was 100 cfu/g) during a 42-week period. Peak rates of C. jejuni isolation (approximately 25% of hens positive) occurred at two different times, in October and in late April to early...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Doyle MP

    更新日期:1984-03-01 00:00:00

  • Metabolism of phosphonoacetate as the sole carbon and phosphorus source by an environmental bacterial isolate.

    abstract::A gram-negative bacterium isolated from activated sludge was able to utilize up to 25 mM phosphonoacetate as the sole carbon and phosphorus source, with simultaneous excretion of virtually equimolar levels of phosphate. 2-Aminoethylphosphonate was similarly utilized with equivalent growth rates and cellular yields, wh...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: McMullan G,Harrington F,Quinn JP

    更新日期:1992-04-01 00:00:00

  • acI Actinobacteria Assemble a Functional Actinorhodopsin with Natively Synthesized Retinal.

    abstract::Freshwater lakes harbor complex microbial communities, but these ecosystems are often dominated by acI Actinobacteria Members of this cosmopolitan lineage are proposed to bolster heterotrophic growth using phototrophy because their genomes encode actino-opsins (actR). This model has been difficult to validate experime...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Dwulit-Smith JR,Hamilton JJ,Stevenson DM,He S,Oyserman BO,Moya-Flores F,Garcia SL,Amador-Noguez D,McMahon KD,Forest KT

    更新日期:2018-11-30 00:00:00

  • Inhibitory Effects of Turf Pesticides on Bacillus popilliae and the Prevalence of Milky Disease.

    abstract::Fourteen pesticides (fungicides, herbicides, and insecticides) were tested to determine whether they had deleterious effects on the bioinsecticide Bacillus popilliae, the causal agent of milky disease. All of these pesticides reduced levels of spore viability, spore germination, and/or vegetative cell growth when they...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Dingman DW

    更新日期:1994-07-01 00:00:00

  • Outermost-cell-surface changes in an encapsulated strain of Staphylococcus aureus after preservation by freeze-drying.

    abstract::The effects of drying time during freeze-drying on the outermost cell surface of an encapsulated strain of Staphylococcus aureus S-7 (Smith, diffuse) were investigated, with special attention paid to capsule and slime production. To quantify capsule and slime production, capsule antigen production and cellular charact...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Ohtomo T,Yamada T,Yoshida K

    更新日期:1988-10-01 00:00:00

  • Differential sunlight sensitivity of picophytoplankton from surface Mediterranean Coastal Waters.

    abstract::We tested the sensitivity of coastal picophytoplankton exposed to natural sunlight in short-term experiments. Cell abundance and cell-specific chlorophyll fluorescence were significantly reduced in Prochlorococcus spp. but not in Synechococcus, whereas picoeukaryotes had an intermediate response. These results are the...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Sommaruga R,Hofer JS,Alonso-Sáez L,Gasol JM

    更新日期:2005-04-01 00:00:00

  • Assessing Transmission of Antimicrobial-Resistant Escherichia coli in Wild Giraffe Contact Networks.

    abstract::There is growing evidence that anthropogenic sources of antibiotics and antimicrobial-resistant bacteria can spill over into natural ecosystems, raising questions about the role wild animals play in the emergence, maintenance, and dispersal of antibiotic resistance genes. In particular, we lack an understanding of how...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Miller EA,Johnson TJ,Omondi G,Atwill ER,Isbell LA,McCowan B,VanderWaal K

    更新日期:2018-12-13 00:00:00

  • Development of a direct in situ PCR method for detection of specific bacteria in natural environments.

    abstract::We applied HNPP (2-hydroxy-3-naphthoic acid-2'-phenylanilide phosphate) to direct in situ PCR for the routine detection of specific bacterial cells at the single-cell level. PCR was performed on glass slides with digoxigenin-labeled dUTP. The digoxigenin-labeled PCR products were detected with alkaline phosphatase-lab...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Tani K,Kurokawa K,Nasu M

    更新日期:1998-04-01 00:00:00

  • Gene transfer by transduction in the marine environment.

    abstract::To determine the potential for bacteriophage-mediated gene transfer in the marine environment, we established transduction systems by using marine phage host isolates. Plasmid pQSR50, which contains transposon Tn5 and encodes kanamycin and streptomycin resistance, was used in plasmid transduction assays. Both marine b...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Jiang SC,Paul JH

    更新日期:1998-08-01 00:00:00

  • Vibrio harveyi nitroreductase is also a chromate reductase.

    abstract::The chromate reductase purified from Pseudomonas ambigua was found to be homologous with several nitroreductases. Escherichia coli DH5alpha and Vibrio harveyi KCTC 2720 nitroreductases were chosen for the present study, and their chromate-reducing activities were determined. A fusion between glutathione S-transferase ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Kwak YH,Lee DS,Kim HB

    更新日期:2003-08-01 00:00:00

  • Relative importance of fluorescent siderophores and other factors in biological control of Gaeumannomyces graminis var. tritici by Pseudomonas fluorescens 2-79 and M4-80R.

    abstract::Pseudomonas fluorescens 2-79 suppresses take-all, a major root disease of wheat caused by Gaeumannomyces graminis var. tritici. The bacteria produce an antibiotic, phenazine-1-carboxylic acid (PCA), and a fluorescent pyoverdin siderophore. Previous studies have established that PCA has an important role in the biologi...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Hamdan H,Weller DM,Thomashow LS

    更新日期:1991-11-01 00:00:00

  • A new Klebsiella planticola strain (Cd-1) grows anaerobically at high cadmium concentrations and precipitates cadmium sulfide.

    abstract::Heavy metal resistance by bacteria is a topic of much importance to the bioremediation of contaminated soils and sediments. We report here the isolation of a highly cadmium-resistant Klebsiella planticola strain, Cd-1, from reducing salt marsh sediments. The strain grows in up to 15 mM CdCl(2) under a wide range of Na...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Sharma PK,Balkwill DL,Frenkel A,Vairavamurthy MA

    更新日期:2000-07-01 00:00:00

  • Effects of High Pressure on Inactivation Kinetics and Events Related to Proton Efflux in Lactobacillus plantarum.

    abstract::Knowledge of the mechanism of pressure-induced inactivation of microorganisms could be helpful in defining an effective, relatively mild pressure treatment as a means of decontamination, especially in combination with other physical treatments or antimicrobial agents. We have studied the effect of high pressure on Lac...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Wouters PC,Glaasker E,Smelt JP

    更新日期:1998-02-01 00:00:00

  • Isolation of Aeromonas spp. from water by using anaerobic incubation.

    abstract::A membrane filtration method incorporating a combination of anaerobic and aerobic incubation has been developed for the enumeration of Aeromonas spp. in drinking water. The use of anaerobic incubation improved the detection of Aeromonas spp. by reducing the growth of nonaeromonads. The confirmation rate of presumptive...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Cunliffe DA,Adcock P

    更新日期:1989-09-01 00:00:00

  • Biodiversity of gas vacuolate bacteria from Antarctic sea ice and water.

    abstract::Psychrophilic, gas vacuolate, heterotrophic bacteria indigenous to sea ice communities in Antarctica have been isolated. Phylogenetic analysis of representative members of these bacteria shows that they belong to the alpha, beta, and gamma Proteobacteria and the Flavobacteria-Cytophaga group. This is the first report ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Gosink JJ,Staley JT

    更新日期:1995-09-01 00:00:00

  • Isolation and identification of rhizoxin analogs from Pseudomonas fluorescens Pf-5 by using a genomic mining strategy.

    abstract::The products synthesized from a hybrid polyketide synthase/nonribosomal peptide synthetase gene cluster in the genome of Pseudomonas fluorescens Pf-5 were identified using a genomics-guided strategy involving insertional mutagenesis and subsequent metabolite profiling. Five analogs of rhizoxin, a 16-member macrolide w...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Loper JE,Henkels MD,Shaffer BT,Valeriote FA,Gross H

    更新日期:2008-05-01 00:00:00

  • Mesophilic and psychrotrophic bacteria from meat and their spoilage potential in vitro and in beef.

    abstract::Mesophilic and psychrotrophic populations from refrigerated meat were identified in this study, and the spoilage potential of microbial isolates in packaged beef was evaluated by analyzing the release of volatile organic compounds (VOC) by gas chromatography-mass spectrometry (GC/MS). Fifty mesophilic and twenty-nine ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Ercolini D,Russo F,Nasi A,Ferranti P,Villani F

    更新日期:2009-04-01 00:00:00

  • Isolation of novel extreme-tolerant cyanobacteria from a rock-dwelling microbial community by using exposure to low Earth orbit.

    abstract::Many cyanobacteria are known to tolerate environmental extremes. Motivated by an interest in selecting cyanobacteria for applications in space, we launched rocks from a limestone cliff in Beer, Devon, United Kingdom, containing an epilithic and endolithic rock-dwelling community of cyanobacteria into low Earth orbit (...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Olsson-Francis K,de la Torre R,Cockell CS

    更新日期:2010-04-01 00:00:00

  • Melanin Produced by the Fast-Growing Marine Bacterium Vibrio natriegens through Heterologous Biosynthesis: Characterization and Application.

    abstract::Melanin is a pigment produced by organisms throughout all domains of life. Due to its unique physicochemical properties, biocompatibility, and biostability, there has been an increasing interest in the use of melanin for broad applications. In the vast majority of studies, melanin has been either chemically synthesize...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Wang Z,Tschirhart T,Schultzhaus Z,Kelly EE,Chen A,Oh E,Nag O,Glaser ER,Kim E,Lloyd PF,Charles PT,Li W,Leary D,Compton J,Phillips DA,Dhinojwala A,Payne GF,Vora GJ

    更新日期:2020-02-18 00:00:00

  • Oxidation of Nitrapyrin to 6-Chloropicolinic Acid by the Ammonia-Oxidizing Bacterium Nitrosomonas europaea.

    abstract::Suspensions of Nitrosomonas europaea catalyzed the oxidation of the commercial nitrification inhibitor nitrapyrin [2-chloro-6-(trichloromethyl)-pyridine]. Rapid oxidation of nitrapyrin (at a concentration of 10 muM) required the concomitant oxidation of ammonia, hydroxylamine, or hydrazine. The turnover rate was highe...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Vannelli T,Hooper AB

    更新日期:1992-07-01 00:00:00

  • Reductive dehalogenation and mineralization of 3-chlorobenzoate in the presence of sulfate by microorganisms from a methanogenic aquifer.

    abstract::We investigated the anaerobic biodegradation of 3-chlorobenzoate (3CBz) by microorganisms from an aquifer where chloroaromatic compounds were previously found to resist decay in the presence of sulfate. After a lengthy lag period, 3CBz was degraded in the presence of sulfate and concurrently with sulfate reduction. Ch...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Townsend GT,Ramanand K,Suflita JM

    更新日期:1997-07-01 00:00:00

  • Preferential binding of radiolabeled zearalenone to a protein fraction of Fusarium roseum graminearum.

    abstract::Zearalenone [6-(10-hydroxy-6-oxo-trans-1-undecenyl)beta-resorcyclic acid lactone] is a hormone produced by Fusarium spp. which regulates the sexual stage in F. roseum. 3H- and 14C-labeled zearalenone were found to bind preferentially to one of two peaks containing uncharacterized proteins obtained from the cytosol of ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Inaba T,Mirocha CJ

    更新日期:1979-01-01 00:00:00

  • Complete reductive dehalogenation of brominated biphenyls by anaerobic microorganisms in sediment.

    abstract::We sought to determine whether microorganisms from the polychlorinated biphenyl (PCB)-contaminated sediment in Woods Pond (Lenox, Mass.) could dehalogenate brominated biphenyls. The PCB dechlorination specificities for the microorganisms in this sediment have been well characterized. This allowed us to compare the deh...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Bedard DL,Van Dort HM

    更新日期:1998-03-01 00:00:00

  • Vibrio parahaemolyticus and Vibrio vulnificus Recovered from Oysters during an Oyster Relay Study.

    abstract::Vibrio parahaemolyticus and Vibrio vulnificus are naturally occurring estuarine bacteria and are the leading causes of seafood-associated infections and mortality in the United States. Though multiple-antibiotic-resistant V. parahaemolyticus and V. vulnificus strains have been reported, resistance patterns in vibrios ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Elmahdi S,Parveen S,Ossai S,DaSilva LV,Jahncke M,Bowers J,Jacobs J

    更新日期:2018-01-17 00:00:00

  • Improved medium for selective isolation and enumeration of Bifidobacterium.

    abstract::Petuely's selective medium for Bifidobacterium was improved by addition of riboflavin, nucleic acid bases, pyruvic acid, and nalidixic acid. The modified medium, when examined under strictly anaerobic conditions for efficient isolation of Bifidobacterium from human fecal samples, exhibited selective and high viable co...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Tanaka R,Mutai M

    更新日期:1980-11-01 00:00:00

  • Identification of a universally primed-PCR-derived sequence-characterized amplified region marker for an antagonistic strain of Clonostachys rosea and development of a strain-specific PCR detection assay.

    abstract::We developed a PCR detection method that selectively recognizes a single biological control agent and demonstrated that universally primed PCR (UP-PCR) can identify strain-specific markers. Antagonistic strains of Clonostachys rosea (syn. Gliocladium roseum) were screened by UP-PCR, and a strain-specific marker was id...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Bulat SA,Lübeck M,Alekhina IA,Jensen DF,Knudsen IM,Lübeck PS

    更新日期:2000-11-01 00:00:00

  • Comparison of yeast growth in mesquite wood hydrolysate.

    abstract::Hot-water extracts of mesquite (Prosopis glandulosa) wood were assayed for their total carbohydrate, reducing sugar, and glucose content. These hydrolysates were then used as complete media for yeast growth. A total of 10 strains of yeasts were evaluated for their biomass production in the mesquite wood hydrolysates. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Stanlake GJ

    更新日期:1986-08-01 00:00:00

  • Detection of Vibrio vulnificus biotypes 1 and 2 in eels and oysters by PCR amplification.

    abstract::DNA extraction procedures and PCR conditions to detect Vibrio vulnificus cells naturally occurring in oysters were developed. In addition, PCR amplification of V. vulnificus from oysters seeded with biotype 1 cells was demonstrated. By the methods described, V. vulnificus cells on a medium (colistin-polymyxin B-cellob...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Coleman SS,Melanson DM,Biosca EG,Oliver JD

    更新日期:1996-04-01 00:00:00

  • Split-Root Assays Using Trifolium subterraneum Show that Rhizobium Infection Induces a Systemic Response That Can Inhibit Nodulation of Another Invasive Rhizobium Strain.

    abstract::Subterranean clover plants possessing two equally infectible and robust lateral root systems ("split roots") were used in conjunction with several specific mutant strains (derived from Rhizobium trifolii ANU843) to investigate a systemic plant response induced by infective Rhizobium strains. This plant response contro...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Sargent L,Huang SZ,Rolfe BG,Djordjevic MA

    更新日期:1987-07-01 00:00:00

  • Clostridium acetobutylicum Protoplast Formation and Regeneration.

    abstract::Techniques and media for the production and regeneration of stable Clostridium acetobutylicum protoplasts are described. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Allcock ER,Reid SJ,Jones DT,Woods DR

    更新日期:1982-03-01 00:00:00