Development and application of monoclonal antibodies for in situ detection of indigenous bacterial strains in aquatic ecosystems.

abstract：:Recovery results of Staphylococcus aureus ATCC 6538 treated with phenolics and quaternary ammonium compounds on Dey and Engley (D/E) neutralizing medium at various time intervals were compared by the use of two commonly used media. Two recovery processes were utilized. In one, the chemically treated organisms were pla...

journal_title：Applied and environmental microbiology

authors： Dey BP,Engley FB Jr

更新日期：1983-05-01 00:00:00

abstract：:The biosynthesis of the major carotenoid spirilloxanthin by the purple nonsulfur bacterium Rhodospirillum rubrum is thought to occur via a linear pathway proceeding through phytoene and, later, lycopene as intermediates. This assumption is based solely on early chemical evidence (B. H. Davies, Biochem. J. 116:93-99, 1...

journal_title：Applied and environmental microbiology

authors： Wang GS,Grammel H,Abou-Aisha K,Sägesser R,Ghosh R

更新日期：2012-10-01 00:00:00

abstract：:Borrelia spirochetes in bird-feeding ticks were studied in the Czech Republic. During the postbreeding period (July to September 2005), 1,080 passerine birds infested by 2,240 Ixodes ricinus subadult ticks were examined. Borrelia garinii was detected in 22.2% of the ticks, Borrelia valaisiana was detected in 12.8% of ...

journal_title：Applied and environmental microbiology

authors： Dubska L,Literak I,Kocianova E,Taragelova V,Sychra O

更新日期：2009-02-01 00:00:00

abstract：:DNA probes that are specific for each of five predominant species of human colonic Bacteroides (B. thetaiotaomicron, B. uniformis, B. distasonis, "Bacteroides group 3452-A", and B. ovatus) were used to detect and enumerate these species in fecal samples from two adult volunteers. These five species are capable of ferm...

journal_title：Applied and environmental microbiology

authors： Kuritza AP,Shaughnessy P,Salyers AA

更新日期：1986-02-01 00:00:00

abstract：:Thiosphaera pantotropha is capable of simultaneous heterotrophic nitrification and aerobic denitrification. Consequently, its nitrification potential could not be judged from nitrite accumulation, but was estimated from complete nitrogen balances. The maximum rate of nitrification obtained during these experiments was...

journal_title：Applied and environmental microbiology

authors： Robertson LA,van Niel EW,Torremans RA,Kuenen JG

更新日期：1988-11-01 00:00:00

abstract：:Blastobacter spp. are freshwater bacteria that form rosette structures by cellular attachment to a common base. Comparative analyses of ribosomal 16S rRNA gene and internally transcribed spacer region sequences indicated that B. denitrificans is a member of the alpha-subdivision of Proteobacteria. Among the alpha-Prot...

journal_title：Applied and environmental microbiology

authors： van Berkum P,Eardly BD

更新日期：2002-03-01 00:00:00

abstract：:Toluene dioxygenase from Pseudomonas putida F1 has been implicated as an enzyme capable of degrading trichloroethylene. This has now been confirmed with Escherichia coli JM109(pDTG601) that contains the structural genes (todC1C2BA) of toluene dioxygenase under the control of the tac promoter. The extent of trichloroet...

journal_title：Applied and environmental microbiology

authors： Zylstra GJ,Wackett LP,Gibson DT

更新日期：1989-12-01 00:00:00

abstract：:Predictive models, based on environmental and water quality variables, have been used to improve the timeliness and accuracy of recreational water quality assessments, but their effectiveness has not been studied in inland waters. Sampling at eight inland recreational lakes in Ohio was done in order to investigate usi...

journal_title：Applied and environmental microbiology

authors： Francy DS,Stelzer EA,Duris JW,Brady AM,Harrison JH,Johnson HE,Ware MW

更新日期：2013-03-01 00:00:00

abstract：:3'-Phosphoadenosine-5'-phosphatase (PAPase) is required for the removal of toxic 3'-phosphoadenosine-5'-phosphate (PAP) produced during sulfur assimilation in various eukaryotic organisms. This enzyme is a well-known target of lithium and sodium toxicity and has been used for the production of salt-resistant transgeni...

journal_title：Applied and environmental microbiology

authors： Zhang JY,Zou J,Bao Q,Chen WL,Wang L,Yang H,Zhang CC

更新日期：2006-01-01 00:00:00

abstract：:Polyester-rayon blend wipes were evaluated for efficiency of extraction and recovery of powdered Bacillus atrophaeus spores from stainless steel and painted wallboard surfaces. Method limits of detection were also estimated for both surfaces. The observed mean efficiency of polyester-rayon blend wipe recovery from sta...

journal_title：Applied and environmental microbiology

authors： Brown GS,Betty RG,Brockmann JE,Lucero DA,Souza CA,Walsh KS,Boucher RM,Tezak M,Wilson MC,Rudolph T

更新日期：2007-02-01 00:00:00

abstract：:We identified a polyketide synthase (PKS) gene, pksN, from a strain of Nectria haematococca by complementing a mutant unable to synthesize a red perithecial pigment. pksN encodes a 2,106-amino-acid polypeptide with conserved motifs characteristic of type I PKS enzymatic domains: beta-ketoacyl synthase, acyltransferase...

journal_title：Applied and environmental microbiology

authors： Graziani S,Vasnier C,Daboussi MJ

更新日期：2004-05-01 00:00:00

abstract：:Indirect exposure to waterborne viruses increases the risk of infection, especially among children with frequent hand-to-mouth contacts. Here, we quantified the transfer of one bacteriophage (MS2) and two enteric viruses (adenovirus and coxsackievirus) from liquid to skin. MS2, a commonly used enteric virus surrogate,...

journal_title：Applied and environmental microbiology

authors： Pitol AK,Bischel HN,Boehm AB,Kohn T,Julian TR

更新日期：2018-10-30 00:00:00

abstract：:Vegetative cells of Bacillus cereus were subjected to low doses of nisin (0.06 microg/ml) and mild pulsed-electric field treatment (16.7 kV/cm, 50 pulses each of 2-micros duration). Combining both treatments resulted in a reduction of 1.8 log units more than the sum of the reductions obtained with the single treatment...

journal_title：Applied and environmental microbiology

authors： Pol IE,Mastwijk HC,Bartels PV,Smid EJ

更新日期：2000-01-01 00:00:00

abstract：:A number of economic and product quality advantages exist in brewing when high-gravity worts of 16 to 18% dissolved solids are fermented. Above this level, production problems such as slow or stuck fermentations and poor yeast viability occur. Ethanol toxicity has been cited as the main cause, as brewers' yeasts are r...

journal_title：Applied and environmental microbiology

authors： Casey GP,Magnus CA,Ingledew WM

更新日期：1984-09-01 00:00:00

abstract：:The strain Burkholderia cepacia G4 aerobically mineralized trichloroethene (TCE) to CO(2) over a time period of approximately 20 h. Three biodegradation experiments were conducted with different bacterial optical densities at 540 nm (OD(540)s) in order to test whether isotope fractionation was consistent. The resultin...

journal_title：Applied and environmental microbiology

authors： Barth JA,Slater G,Schüth C,Bill M,Downey A,Larkin M,Kalin RM

更新日期：2002-04-01 00:00:00

abstract：:Characterization by partial 16S rRNA gene sequencing, ribotyping, and green fluorescent protein-based nisin bioassay revealed that 6 of 20 human milk samples contained nisin-producing Lactococcus lactis bacteria. This suggests that the history of humans consuming nisin is older than the tradition of consuming fermente...

journal_title：Applied and environmental microbiology

authors： Beasley SS,Saris PE

更新日期：2004-08-01 00:00:00

abstract：:Identifying and overcoming the limitations preventing efficient high-yield production of chemicals remain important tasks in metabolic engineering. In an attempt to rewire Corynebacterium glutamicum to produce ethanol, we attained a low yield (63% of the theoretical) when using resting cells on glucose, and large amou...

journal_title：Applied and environmental microbiology

authors： Shen J,Chen J,Solem C,Jensen PR,Liu JM

更新日期：2020-11-24 00:00:00

abstract：:The nprA gene, encoding Bacillus thuringiensis neutral protease A, was cloned by the use of gene-specific oligonucleotides. The size of neutral protease A deduced from the nprA sequence was 566 amino acids (60,982 Da). The cloned nprA gene was partially deleted in vitro, and the deleted allele, designated nprA3, was u...

journal_title：Applied and environmental microbiology

authors： Donovan WP,Tan Y,Slaney AC

更新日期：1997-06-01 00:00:00

abstract：:Cotton flowers in replicate plots in two fields near Phoenix, Ariz., were tagged in June at the beginning of the flowering period. Flowers or developing bolls from these tagged flowers were inoculated on the involucral (bracteal) nectaries with dry spores of Aspergillus flavus. The bolls were harvested as they matured...

journal_title：Applied and environmental microbiology

authors： Klich MA,Chmielewski MA

更新日期：1985-09-01 00:00:00

abstract：:When carbon sources are changed, Saccharomyces cerevisiae transcriptional patterns drastically change. To identify genes whose transcription can be used to quantitatively measure sugar concentrations, we searched genomic expression databases for a set of genes that are highly induced during the diauxic shift, and we u...

journal_title：Applied and environmental microbiology

authors： Chambers P,Issaka A,Palecek SP

更新日期：2004-01-01 00:00:00

abstract：:The bphC and bphD genes of Pseudomonas putida involved in the catabolism of polychlorinated biphenyls or biphenyl were identified, localized, and studied for expression in Escherichia coli. This was achieved by cloning a 2.4-kilobase (kb) DNA fragment of recombinant cosmid pOH101 into HindIII site of pUC plasmids down...

journal_title：Applied and environmental microbiology

authors： Khan AA,Walia SK

更新日期：1990-04-01 00:00:00

abstract：:A "repair-detection" procedure consisting of pour plating of food samples with Trypticase soy agar, followed by 1-h repair incubation at room temperature and subsequent overlay with violet red bile agar, was found to be an effective method for the detection of injuried and uninjuried coliforms from dairy products. Thi...

journal_title：Applied and environmental microbiology

authors： Ray B,Speck ML

更新日期：1978-04-01 00:00:00

abstract：:Oysters collected in late winter, when they were free of Vibrio vulnificus, were exposed in the organism in the laboratory. The oysters effectively concentrated the bacteria from seawater, but when the inoculum was removed, the bacteria were rapidly cleared from the oyster tissues. These results suggest that V. vulnif...

journal_title：Applied and environmental microbiology

authors： Kelly MT,Dinuzzo A

更新日期：1985-12-01 00:00:00

abstract：:Antigens from the methicillin-resistant Staphylococcus aureus (MRSA) cell wall have been shown to be immunogenic in vivo and upregulated during biofilm growth. In this study, we created purified, recombinant forms of selected antigens and biofilm-upregulated, cell wall-associated proteins. These proteins were shown to...

journal_title：Applied and environmental microbiology

authors： Brady RA,Leid JG,Kofonow J,Costerton JW,Shirtliff ME

更新日期：2007-10-01 00:00:00

abstract：:High-performance liquid chromatography with a cation-exchange resin-packed column was used to determine fermentation products of several known and unknown Clostridium species. The column was operated at 30 degrees C, and isocratic elution was done with 0.013 N H(2)SO(4). Sample preparation for high-performance liquid ...

journal_title：Applied and environmental microbiology

authors： Ehrlich GG,Goerlitz DF,Bourell JH,Eisen GV,Godsy EM

更新日期：1981-11-01 00:00:00

abstract：:[This corrects the article on p. 514 in vol. 55.]. ...

journal_title：Applied and environmental microbiology

authors：

更新日期：1989-06-01 00:00:00

abstract：:Ground bark and heartwood from Alnus rubra and Pseudotsuga menziesii were added to a muddy sediment from a small Oregon stream and incubated in situ. Carbon dioxide and methane production rates were increased by all amendments, the biggest increase being shown with A. rubra wood. Except for sediment amended with A. ru...

journal_title：Applied and environmental microbiology

authors： Baker JH,Morita RY,Anderson NH

更新日期：1983-02-01 00:00:00

abstract：:To ascertain its physiological similarity to other methanogenic bacteria, Methanospirillum hungatii, the type species of the genus, was characterized nutritionally and biochemically. Good growth occurred in a medium consisting of mineral salts, cysteine sulfide reducing buffer, and an H2-CO2 (80:20) atmosphere. Additi...

journal_title：Applied and environmental microbiology

authors： Ferry JG,Wolfe RS

更新日期：1977-10-01 00:00:00

abstract：:Light-dependent regulation of cell cycle progression in the marine cyanobacterium Synechococcus strain WH-8101 was demonstrated through the use of flow cytometry. Our results show that, similar to eucaryotic cells, marine Synechococcus spp. display two gaps in DNA synthesis, at the beginning and at the end of the cell...

journal_title：Applied and environmental microbiology

authors： Armbrust EV,Bowen JD,Olson RJ,Chisholm SW

更新日期：1989-02-01 00:00:00

abstract：:Effective wastewater management is crucial to ensure the safety of water reuse projects and effluent discharge into surface waters. Multiple studies have demonstrated that municipal wastewater treatment with conventional activated sludge processes is inefficient for the removal of a wide spectrum of viruses in sewage....

journal_title：Applied and environmental microbiology

authors： Chacón L,Barrantes K,Santamaría-Ulloa C,Solano M,Reyes L,Taylor L,Valiente C,Symonds EM,Achí R

更新日期：2020-08-18 00:00:00