Plating procedure for the enumeration of coliforms from dairy products.

abstract：:The mercury resistance (mer) operon of plasmid R100 was cloned onto various plasmid vectors to study the effect of mer gene amplification on the rate of Hg2+ reduction by Escherichia coli cells. The plasmids were maintained at copy numbers ranging from 3 to 140 copies per cell. The overall Hg2+ reduction rate of intac...

journal_title：Applied and environmental microbiology

authors： Philippidis GP,Malmberg LH,Hu WS,Schottel JL

更新日期：1991-12-01 00:00:00

abstract：:Bacterial diversity in a deep-sea sediment was investigated by constructing actinobacterium-specific 16S ribosomal DNA (rDNA) clone libraries from sediment sections taken 5 to 12, 15 to 18, and 43 to 46 cm below the sea floor at a depth of 3,814 m. Clones were placed into operational taxonomic unit (OTU) groups with >...

journal_title：Applied and environmental microbiology

authors： Stach JE,Maldonado LA,Masson DG,Ward AC,Goodfellow M,Bull AT

更新日期：2003-10-01 00:00:00

abstract：:The possible existence of endemism among microorganisms resulting from and preserved by geographic isolation is one of the most controversial topics in microbial ecology. We isolated 31 strains of "Spumella-like" flagellates from remote sampling sites from all continents, including Antarctica. These and another 23 iso...

journal_title：Applied and environmental microbiology

authors： Boenigk J,Pfandl K,Garstecki T,Harms H,Novarino G,Chatzinotas A

更新日期：2006-08-01 00:00:00

abstract：:Phenolic compounds are believed to be promising candidates as complementary therapeutics. Maple syrup, prepared by concentrating the sap from the North American maple tree, is a rich source of natural and process-derived phenolic compounds. In this work, we report the antimicrobial activity of a phenolic-rich maple sy...

journal_title：Applied and environmental microbiology

authors： Maisuria VB,Hosseinidoust Z,Tufenkji N

更新日期：2015-06-01 00:00:00

abstract：:Three species of colonic bacteria can ferment the mucopolysaccharide chondroitin sulfate: Bacteroides ovatus, Bacteroides sp. strain 3452A (an unnamed DNA homology group), and B. thetaiotaomicron. Proteins associated with the utilization of chondroitin sulfate by B. thetaiotaomicron have been characterized previously....

journal_title：Applied and environmental microbiology

authors： Lipeski L,Guthrie EP,O'Brien M,Kotarski SF,Salyers AA

更新日期：1986-05-01 00:00:00

abstract：:Of 199 fungal cultures isolated from some of the common cereals collected from different parts of Uttar Pradesh and Madhya Pradesh, 70 produced toxic metabolites. Of the 70 fungi isolated, 59 produced toxins which caused visible lesions in livers, kidneys, and spleens but did not cause mortality. Toxicity, graded in t...

journal_title：Applied and environmental microbiology

authors： Gupta J,Pathak B,Sethi N,Vora VC

更新日期：1981-03-01 00:00:00

abstract：:"Candidatus Methanoperedens nitroreducens" is an archaeon that couples the anaerobic oxidation of methane to nitrate reduction. In natural and man-made ecosystems, this archaeon is often found at oxic-anoxic interfaces where nitrate, the product of aerobic nitrification, cooccurs with methane produced by methanogens. ...

journal_title：Applied and environmental microbiology

authors： Guerrero-Cruz S,Cremers G,van Alen TA,Op den Camp HJM,Jetten MSM,Rasigraf O,Vaksmaa A

更新日期：2018-11-30 00:00:00

abstract：:The fate of Listeria monocytogenes during refrigerated storage was determined on several processed meat products, including ham, bologna, wieners, sliced chicken, sliced turkey, fermented semidried sausage, bratwurst, and cooked roast beef. The meats were surface inoculated with a five-strain mixture of less than or e...

journal_title：Applied and environmental microbiology

authors： Glass KA,Doyle MP

更新日期：1989-06-01 00:00:00

abstract：:A 2.2-kb fragment containing a replicon from pBtoxis, the large plasmid that encodes the insecticidal endotoxins of Bacillus thuringiensis subsp. israelensis, was identified, cloned, and sequenced. This fragment contains cis elements, including iterons, found in replication origins of other large plasmids and suggests...

journal_title：Applied and environmental microbiology

authors： Tang M,Bideshi DK,Park HW,Federici BA

更新日期：2006-11-01 00:00:00

abstract：:A method was developed to follow bacterial nitrate reduction in freshwater sediments by using common high-performance liquid chromatographic equipment. The low detection limit (14 pmol) of the method enabled us to study concentration profiles and reaction kinetics under natural conditions. Significant nitrate concentr...

journal_title：Applied and environmental microbiology

authors： Hordijk CA,Snieder M,van Engelen JJ,Cappenberg TE

更新日期：1987-02-01 00:00:00

abstract：:Subterranean clover plants possessing two equally infectible and robust lateral root systems ("split roots") were used in conjunction with several specific mutant strains (derived from Rhizobium trifolii ANU843) to investigate a systemic plant response induced by infective Rhizobium strains. This plant response contro...

journal_title：Applied and environmental microbiology

authors： Sargent L,Huang SZ,Rolfe BG,Djordjevic MA

更新日期：1987-07-01 00:00:00

abstract：:During fermentative growth in natural and industrial environments, Saccharomyces cerevisiae must redistribute the available nitrogen from multiple exogenous sources to amino acids in order to suitably fulfill anabolic requirements. To exhaustively explore the management of this complex resource, we developed an advanc...

journal_title：Applied and environmental microbiology

authors： Crépin L,Truong NM,Bloem A,Sanchez I,Dequin S,Camarasa C

更新日期：2017-02-15 00:00:00

abstract：:Many fungal pathogens have short generation times, large population sizes, and mixed reproductive systems, providing high potential to adapt to heterogeneous environments of agroecosystems. Such adaptation complicates disease management and threatens food production. A better understanding of pathogen population biolo...

journal_title：Applied and environmental microbiology

authors： Kozhar O,Larsen MM,Grünwald NJ,Peever TL

更新日期：2020-04-17 00:00:00

abstract：:Five different bacterial extracellular polymer extraction methods and a combination of two of these methods were compared on cultures of activated sludge, synthetic activated sludge, and Klebsiella aerogenes. High-speed centrifugation was the most effective extraction method for the K. aerogenes culture, based on the ...

journal_title：Applied and environmental microbiology

authors： Brown MJ,Lester JN

更新日期：1980-08-01 00:00:00

abstract：:This study was undertaken to find optimum conditions of tryptone, yeast extract, glucose, Tween 80, and incubation temperature for the growth of Lactobacillus casei YIT 9018 and to assess the effects of these factors by use of response surface methodology. A central composite design was used as an experimental design ...

journal_title：Applied and environmental microbiology

authors： Oh S,Rheem S,Sim J,Kim S,Baek Y

更新日期：1995-11-01 00:00:00

abstract：:Free fatty acids are important flavor compounds in cheese. Propionibacterium freudenreichii is the main agent of their release through lipolysis in Swiss cheese. Our aim was to identify the esterase(s) involved in lipolysis by P. freudenreichii. We targeted two previously identified esterases: one secreted esterase, P...

journal_title：Applied and environmental microbiology

authors： Abeijón Mukdsi MC,Falentin H,Maillard MB,Chuat V,Medina RB,Parayre S,Thierry A

更新日期：2014-01-01 00:00:00

abstract：:Lantibiotics are ribosomally synthesized antimicrobial peptides with substantial posttranslational modifications. They are characterized by the unique amino acids lanthionine and methyllanthionine, which are introduced by dehydration of Ser/Thr residues and linkage of the resulting dehydrated amino acids with Cys resi...

journal_title：Applied and environmental microbiology

authors： Basi-Chipalu S,Dischinger J,Josten M,Szekat C,Zweynert A,Sahl HG,Bierbaum G

更新日期：2015-05-15 00:00:00

abstract：:We have analyzed the impact of surface-to-volume ratio on final bacterial concentrations after batch growth. We examined six bottle sizes (20 to 1,000 ml) using three independent enumeration methods to quantify growth. We found no evidence of a so-called volumetric bottle effect, thus contradicting numerous previous r...

journal_title：Applied and environmental microbiology

authors： Hammes F,Vital M,Egli T

更新日期：2010-02-01 00:00:00

abstract：:Methanogens can use H(2) produced by cathodic depolarization-mediated oxidation of elemental iron to produce methane. Thermodynamic consideration of the cathodic depolarization mechanism predicts more oxidation of Fe at lower pH. Methanogenic responses to pH by Methanococcus deltae, Methanococcus thermolithotrophicus,...

journal_title：Applied and environmental microbiology

authors： Boopathy R,Daniels L

更新日期：1991-07-01 00:00:00

abstract：:Rabbits have been increasingly linked to the persistence of paratuberculosis (Johne's disease) in domestic ruminants in the United Kingdom. The aims of this study were to determine the routes of intraspecies transmission of Mycobacterium avium subspecies paratuberculosis (MAP) in rabbits and to estimate the probabilit...

journal_title：Applied and environmental microbiology

authors： Judge J,Kyriazakis I,Greig A,Davidson RS,Hutchings MR

更新日期：2006-01-01 00:00:00

abstract：:We evaluated a ready-to-use real-time quantitative Legionella pneumophila PCR assay system by testing 136 hot-water-system samples collected from 55 sites as well as 49 cooling tower samples collected from 20 different sites, in parallel with the standard culture method. The PCR assay was reproducible and suitable for...

journal_title：Applied and environmental microbiology

authors： Yaradou DF,Hallier-Soulier S,Moreau S,Poty F,Hillion Y,Reyrolle M,André J,Festoc G,Delabre K,Vandenesch F,Etienne J,Jarraud S

更新日期：2007-03-01 00:00:00

abstract：:The effects of pulsed electric field (PEF) treatment and processing factors on the inactivation kinetics of Listeria innocua NCTC 11289 were investigated by using a pilot plant PEF unit with a flow rate of 200 liters/h. The electric field strength, pulse length, number of pulses, and inlet temperature were the most si...

journal_title：Applied and environmental microbiology

authors： Wouters PC,Dutreux N,Smelt JP,Lelieveld HL

更新日期：1999-12-01 00:00:00

abstract：:The oral biofilm community consists of >800 microbial species, among which Streptococcus mutans is considered a primary pathogen for dental caries. The genomic island TnSmu2 of S. mutans comprises >2% of the genome. In this study, we demonstrate that TnSmu2 harbors a gene cluster encoding nonribosomal peptide syntheta...

journal_title：Applied and environmental microbiology

authors： Wu C,Cichewicz R,Li Y,Liu J,Roe B,Ferretti J,Merritt J,Qi F

更新日期：2010-09-01 00:00:00

abstract：:Several variations in the scintillation mixture and the filter paper arrangements for double-vial radiorespirometry were compared. Improved efficiencies (44%) and shorter response times were found by adding wetting agents and methanolic NaOH to the scintillation mixture in the filter paper. The scintillation chemicals...

journal_title：Applied and environmental microbiology

authors： McKinley VL,Federle TW,Vestal JR

更新日期：1983-01-01 00:00:00

abstract：:Cowpea is a legume of great agronomic importance that establishes symbiotic relationships with nitrogen-fixing bacteria. However, little is known about the genetic and symbiotic diversity of these bacteria in distinct ecosystems. Our study evaluated the genetic diversity and symbiotic efficiencies of 119 bacterial str...

journal_title：Applied and environmental microbiology

authors： Azarias Guimarães A,Duque Jaramillo PM,Simão Abrahão Nóbrega R,Florentino LA,Barroso Silva K,de Souza Moreira FM

更新日期：2012-09-01 00:00:00

abstract：:A screen of the Saccharomyces cerevisiae deletion strain set was performed to identify genes affecting hydrogen sulfide (H(2)S) production. Mutants were screened using two assays: colony color on BiGGY agar, which detects the basal level of sulfite reductase activity, and production of H(2)S in a synthetic juice mediu...

journal_title：Applied and environmental microbiology

authors： Linderholm AL,Findleton CL,Kumar G,Hong Y,Bisson LF

更新日期：2008-03-01 00:00:00

abstract：:The strictly anaerobic pathogenic bacterium Clostridium difficile occurs in the human gut and is able to thrive from fermentation of leucine. Thereby the amino acid is both oxidized to isovalerate plus CO(2) and reduced to isocaproate. In the reductive branch of this pathway, the dehydration of (R)-2-hydroxyisocaproyl...

journal_title：Applied and environmental microbiology

authors： Kim J,Darley D,Selmer T,Buckel W

更新日期：2006-09-01 00:00:00

abstract：:Phytophthora ramorum is a recently described pathogen causing oak mortality (sudden oak death) in forests in coastal areas of California and southern Oregon and dieback and leaf blight in a range of tree, shrub, and herbaceous species in the United States and Europe. Due to the threat posed by this organism, stringent...

journal_title：Applied and environmental microbiology

authors： Tomlinson JA,Boonham N,Hughes KJ,Griffin RL,Barker I

更新日期：2005-11-01 00:00:00

abstract：:The metabolic conversion of 5-methylfurfural and 2-methylfurfural to furfural by a methanogenic bacterium, Methanococcus sp. strain B, was studied. This bacterium was found to use methylfurfural compounds as a growth substrate and to convert them stoichiometrically to furfural. For every mole of methylfurfurals metabo...

journal_title：Applied and environmental microbiology

authors： Boopathy R

更新日期：1996-09-01 00:00:00

abstract：:The association of Vibrio cholerae with zooplankton has been suggested as an important factor in transmission of human epidemic cholera, and the ability to colonize zooplankton surfaces may play a role in the temporal variation and predominance of the two different serogroups (V. cholerae O1 El Tor and O139) in the aq...

journal_title：Applied and environmental microbiology

authors： Rawlings TK,Ruiz GM,Colwell RR

更新日期：2007-12-01 00:00:00