Minireplicon from pBtoxis of Bacillus thuringiensis subsp. israelensis.

abstract：:A stable bacterial community absorbed and transformed the herbicide metolachlor [2-chloro-N-(2-ethyl- 6-methylphenyl)-N-(2-methoxy-1-methylethyl)-acetamide] from a liquid medium. About 80% of the added ring-[U-C]metolachlor (50 mug/ml) disappeared from the medium and accumulated inside the cells. The ratio of cellular...

journal_title：Applied and environmental microbiology

authors： Liu SY,Zheng Z,Zhang R,Bollag JM

更新日期：1989-03-01 00:00:00

abstract：:Intracellular granules containing ferric and ferrous iron formed in Shewanella putrefaciens CN32 during dissimilatory reduction of solid-phase ferric iron. It is the first in situ detection at high resolution (150 nm) of a mixed-valence metal particle residing within a prokaryotic cell. The relationship of the interna...

journal_title：Applied and environmental microbiology

authors： Glasauer S,Langley S,Boyanov M,Lai B,Kemner K,Beveridge TJ

更新日期：2007-02-01 00:00:00

abstract：:Ten laboratories participated in an interlaboratory comparison of determination of bioleaching rates of a pyrite reference material. A standardized procedure and a single strain of Thiobacillus ferrooxidans were used in this study. The mean rate of bioleaching of the pyrite reference material was 12.4 mg of Fe per lit...

journal_title：Applied and environmental microbiology

authors： Olson GJ

更新日期：1991-03-01 00:00:00

abstract：:Many cyanobacteria are known to tolerate environmental extremes. Motivated by an interest in selecting cyanobacteria for applications in space, we launched rocks from a limestone cliff in Beer, Devon, United Kingdom, containing an epilithic and endolithic rock-dwelling community of cyanobacteria into low Earth orbit (...

journal_title：Applied and environmental microbiology

authors： Olsson-Francis K,de la Torre R,Cockell CS

更新日期：2010-04-01 00:00:00

abstract：:The genus Exiguobacterium can adapt readily to, and survive in, diverse environments. Our study demonstrated that Exiguobacterium sp. strain S3-2, isolated from marine sediment, is resistant to five antibiotics. The plasmid pMC1 in this strain carries seven putative resistance genes. We functionally characterized thes...

journal_title：Applied and environmental microbiology

authors： Yang J,Wang C,Wu J,Liu L,Zhang G,Feng J

更新日期：2014-02-01 00:00:00

abstract：:A dot blot hybridization procedure with 32P-labeled whole chromosomal DNA of the type strains as probes was developed as a rapid and simple method for identification of intestinal Bacteroides species. Bacterial cells were fixed onto membrane filters by slight suction, treated with 0.5 N NaOH, and hybridized with these...

journal_title：Applied and environmental microbiology

authors： Morotomi M,Ohno T,Mutai M

更新日期：1988-05-01 00:00:00

abstract：:The ionic and nonionic surfactants sodium dodecyl sulfate and Triton X-100, respectively, eluted two viruses, phi X174 and PRD1, which were adsorbed to the ionic and nonionic binding membranes cationic polysulfone and nitrocellulose, respectively. Results indicated that complete elution was readily achieved only when ...

journal_title：Applied and environmental microbiology

authors： Fujito BT,Lytle CD

更新日期：1996-09-01 00:00:00

abstract：:Two strains of Vibrio cholerae serotype O1 Inaba were isolated from eastern oysters, Crassostrea virginica, collected from estuarine waters in Florida during April 1980. The oyster meats and waters from which the oysters were collected had low fecal coliform counts, and the area had no prior evidence of sewage contami...

journal_title：Applied and environmental microbiology

authors： Hood MA,Ness GE,Rodrick GE

更新日期：1981-02-01 00:00:00

abstract：:Renibacterium salmoninarum, a gram-positive diplococcobacillus, causes bacterial kidney disease, a condition that can result in extensive morbidity and mortality among stocks of fish. An immunodominant extracellular protein, called major soluble antigen (MSA), is encoded by two identical genes, msa1 and msa2. We found...

journal_title：Applied and environmental microbiology

authors： Rhodes LD,Coady AM,Deinhard RK

更新日期：2004-11-01 00:00:00

abstract：:A previously identified insert expressing an endopeptidase from a Lactobacillus helveticus CNRZ32 genomic library was characterized. Nucleotide sequence analysis revealed an open reading frame of 1,941 bp encoding a putative protein of 71.2 kDa which contained a zinc-protease motif. Protein homology searches revealed ...

journal_title：Applied and environmental microbiology

authors： Chen YS,Steele JL

更新日期：1998-09-01 00:00:00

abstract：:Pseudomonas oleovorans is capable of producing poly(3-hydroxyalkanoates) (PHAs) as intracellular storage material. To analyze the possible involvement of phaD in medium-chain-length (MCL) PHA biosynthesis, we generated a phaD knockout mutant by homologous recombination. Upon disruption of the phaD gene, MCL PHA polyme...

journal_title：Applied and environmental microbiology

authors： Klinke S,de Roo G,Witholt B,Kessler B

更新日期：2000-09-01 00:00:00

abstract：:The dynamic control of gene expression is important for adjusting fluxes in order to obtain desired products and achieve appropriate cell growth, particularly when the synthesis of a desired product drains metabolites required for cell growth. For dynamic gene expression, a promoter responsive to a particular environm...

journal_title：Applied and environmental microbiology

authors： Yin X,Shin HD,Li J,Du G,Liu L,Chen J

更新日期：2017-03-02 00:00:00

abstract：:A bacterial strain able to transform iprodione was isolated from a fast iprodione-degrading soil by enrichment procedures. Transformation was detected through 3,5-dichloroaniline production as measured by a rapid colorimetric method. The strain, MA6, was tentatively identified as an Arthrobacter sp. When it was incuba...

journal_title：Applied and environmental microbiology

authors： Athiel P,Alfizar,Mercadier C,Vega D,Bastide J,Davet P,Brunel B,Cleyet-Marel JC

更新日期：1995-09-01 00:00:00

abstract：:A new primer set was designed to specifically amplify ca. 1,100 bp of aoxB genes encoding the As(III) oxidase catalytic subunit from taxonomically diverse aerobic As(III)-oxidizing bacteria. Comparative analysis of AoxB protein sequences showed variable conservation levels and highlighted the conservation of essential...

journal_title：Applied and environmental microbiology

authors： Quéméneur M,Heinrich-Salmeron A,Muller D,Lièvremont D,Jauzein M,Bertin PN,Garrido F,Joulian C

更新日期：2008-07-01 00:00:00

abstract：:The goal of this field study was to provide insight into three distinct populations of microorganisms involved in in situ metabolism of phenol. Our approach measured 13CO2 respired from [13C]phenol and stable isotope probing (SIP) of soil DNA at an agricultural field site. Traditionally, SIP-based investigations have ...

journal_title：Applied and environmental microbiology

authors： DeRito CM,Pumphrey GM,Madsen EL

更新日期：2005-12-01 00:00:00

abstract：:We describe the development and validation of a method for the qualitative analysis of complex bifidobacterial communities based on PCR and denaturing gradient gel electrophoresis (DGGE). Bifidobacterium genus-specific primers were used to amplify an approximately 520-bp fragment from the 16S ribosomal DNA (rDNA), and...

journal_title：Applied and environmental microbiology

authors： Satokari RM,Vaughan EE,Akkermans AD,Saarela M,de Vos WM

更新日期：2001-02-01 00:00:00

abstract：:Bacillus cereus UW85 produces the linear aminopolyol antibiotic zwittermicin A (ZmA). This antibiotic has diverse biological activities, such as suppression of disease in plants caused by protists, inhibition of fungal and bacterial growth, and amplification of the insecticidal activity of the toxin protein from Bacil...

journal_title：Applied and environmental microbiology

authors： Kevany BM,Rasko DA,Thomas MG

更新日期：2009-02-01 00:00:00

abstract：:The role of polyethylene glycol (PEG) in the transformation of Schizosaccharomyces pombe by electroporation is investigated by fluorescein isothiocyanate-dextran uptake and transformation studies. It is shown that when S. pombe cells are electroporated in the presence of PEG, the permeability state created is sustaine...

journal_title：Applied and environmental microbiology

authors： Hood MT,Stachow C

更新日期：1992-04-01 00:00:00

abstract：:It has been suggested (L. H. Harris, R. P. Desai, N. E. Welker, and E. T. Papoutsakis, Biotechnol. Bioeng. 67:1-11, 2000) that butyryl phosphate (BuP) is a regulator of solventogenesis in Clostridium acetobutylicum. Here, we determined BuP and acetyl phosphate (AcP) levels in fermentations of C. acetobutylicum wild ty...

journal_title：Applied and environmental microbiology

authors： Zhao Y,Tomas CA,Rudolph FB,Papoutsakis ET,Bennett GN

更新日期：2005-01-01 00:00:00

abstract：:We report the construction of an Escherichia coli mutant that harbors two compatible plasmids and that is able to synthesize labeled 2-O-alpha-D-mannosyl-D-glycerate from externally added labeled mannose without the loss of specific isotopic enrichment. The strain carries a deletion in the manA gene, encoding phosphom...

journal_title：Applied and environmental microbiology

authors： Sampaio MM,Santos H,Boos W

更新日期：2003-01-01 00:00:00

abstract：:The methylotrophic yeast Pichia pastoris has been utilized for heterologous protein expression for over 30 years. Because P. pastoris secretes few of its own proteins, the exported recombinant protein is the major polypeptide in the extracellular medium, making purification relatively easy. Unfortunately, some recombi...

journal_title：Applied and environmental microbiology

authors： Naranjo CA,Jivan AD,Vo MN,de Sa Campos KH,Deyarmin JS,Hekman RM,Uribe C,Hang A,Her K,Fong MM,Choi JJ,Chou C,Rabara TR,Myers G,Moua P,Thor,Risser DD,Vierra CA,Franz AH,Lin-Cereghino J,Lin-Cereghino GP

更新日期：2019-11-27 00:00:00

abstract：:Yeasts able to grow on d-xylose were screened for the ability to hydrolyze xylan. Xylanase activity was found to be rare; a total of only 19 of more than 250 strains yielded a positive test result. The activity was localized largely in the genus Cryptococcus and in Pichia stipitis and its anamorph Candida shehatae. Th...

journal_title：Applied and environmental microbiology

authors： Lee H,Biely P,Latta RK,Barbosa MF,Schneider H

更新日期：1986-08-01 00:00:00

abstract：:Water resources contaminated with wastewater are an important source for the dissemination of enteric viruses with an impact on the health of the population. The aim of the study was to assess the viral contamination of freshwater from a dam in Argentina by using infectious enterovirus detection, viral RNA amplificati...

journal_title：Applied and environmental microbiology

authors： Masachessi G,Pisano MB,Prez VE,Martínez LC,Michelena JF,Martínez-Wassaf M,Giordano MO,Isa MB,Pavan JV,Welter A,Nates SV,Ré V

更新日期：2018-02-14 00:00:00

abstract：:DNA dot blot hybridizations with a cryV-specific probe and a cryI-specific probe were performed to screen 24 Bacillus thuringiensis strains for their cryV-type (lepidopteran- and coleopteran-specific) and cryI-type (lepidopteran-specific) insecticidal crystal protein gene contents, respectively. The cryV-specific prob...

journal_title：Applied and environmental microbiology

authors： Shin BS,Park SH,Choi SK,Koo BT,Lee ST,Kim JI

更新日期：1995-06-01 00:00:00

abstract：:The genes encoding NAD(+)-dependent alanine dehydrogenases (AlaDHs) (EC 1.4.1.1) from the Antarctic bacterial organisms Shewanella sp. strain Ac10 (SheAlaDH) and Carnobacterium sp. strain St2 (CarAlaDH) were cloned and expressed in Escherichia coli. Of all of the AlaDHs that have been sequenced, SheAlaDH exhibited the...

journal_title：Applied and environmental microbiology

authors： Galkin A,Kulakova L,Ashida H,Sawa Y,Esaki N

更新日期：1999-09-01 00:00:00

abstract：:Rice blast caused by the phytopathogen Magnaporthe grisea poses a serious threat to global food security and is difficult to control. Bacillus species have been extensively explored for the biological control of many fungal diseases. In the present study, the marine bacterium Bacillus subtilis BS155 showed a strong an...

journal_title：Applied and environmental microbiology

authors： Zhang L,Sun C

更新日期：2018-08-31 00:00:00

abstract：:Clostridium difficile causes a potentially fatal diarrheal disease through the production of its principal virulence factors, toxin A and toxin B. The tcdC gene is thought to encode a negative regulator of toxin production. Therefore, increased toxin production, and hence increased virulence, is often inferred in stra...

journal_title：Applied and environmental microbiology

authors： Cartman ST,Kelly ML,Heeg D,Heap JT,Minton NP

更新日期：2012-07-01 00:00:00

abstract：:The world's largest outbreak of waterborne toxoplasmosis occurred in a municipality in the western Canadian province of British Columbia. When drinking water emerged as a possible source of infection during the outbreak investigation, a laboratory method was needed to attempt detection of the parasite, Toxoplasma gond...

journal_title：Applied and environmental microbiology

authors： Isaac-Renton J,Bowie WR,King A,Irwin GS,Ong CS,Fung CP,Shokeir MO,Dubey JP

更新日期：1998-06-01 00:00:00

abstract：:Hepatitis A virus (HAV) and Norwalk-like virus (NLV) were detected by reverse transcription-PCR in clams imported into the United States from China. An epidemiological investigation showed that these clams were associated with five cases of Norwalk-like gastroenteritis in New York State in August 2000 (Food and Drug A...

journal_title：Applied and environmental microbiology

authors： Kingsley DH,Meade GK,Richards GP

更新日期：2002-08-01 00:00:00

abstract：:The ability of pure cultures of bacteria isolated from spoiling chicken leg muscle to produce strong off-odors was tested by using sterile leg muscle sections. Changes in the flora during storage and the incidence and identity of organisms capable of producing strong off-odors were noted. ...

journal_title：Applied and environmental microbiology

authors： McMeekin TA

更新日期：1977-06-01 00:00:00