Abstract:
:Hepatitis A virus (HAV) and Norwalk-like virus (NLV) were detected by reverse transcription-PCR in clams imported into the United States from China. An epidemiological investigation showed that these clams were associated with five cases of Norwalk-like gastroenteritis in New York State in August 2000 (Food and Drug Administration Import Alert 16-50). They were labeled "cooked" but appeared raw. Viral RNA extraction was performed by using dissected digestive tissues rather than whole shellfish meats; this was followed by glycine buffer elution, polyethylene glycol precipitation, Tri-Reagent treatment, and purification of poly(A) RNA with magnetic beads coupled to poly(dT) oligonucleotides. We identified HAV and NLV as genotype I and genogroup II strains, respectively. Both viruses have high levels of homology to Asian strains. An analysis of fecal coliforms revealed a most-probable number of 93,000/100 g of clam meat, which is approximately 300-fold higher than the hygienic standard for shellfish meats.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Kingsley DH,Meade GK,Richards GPdoi
10.1128/aem.68.8.3914-3918.2002keywords:
subject
Has Abstractpub_date
2002-08-01 00:00:00pages
3914-8issue
8eissn
0099-2240issn
1098-5336journal_volume
68pub_type
杂志文章abstract::A beta-mannanase (EC 3.2.1.78) from Vibrio sp. strain MA-138 was purified by ammonium sulfate precipitation and several chromatographic procedures including gel filtration, adsorption, and ion-exchange chromatographies. The final ion-exchange chromatography Mono Q yielded one major active fraction and three minor acti...
journal_title:Applied and environmental microbiology
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doi:10.1128/AEM.61.12.4454-4458.1995
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doi:10.1128/AEM.40.3.507-510.1980
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更新日期:2006-06-01 00:00:00
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更新日期:1992-11-01 00:00:00
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更新日期:2006-07-01 00:00:00
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更新日期:2001-02-01 00:00:00
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journal_title:Applied and environmental microbiology
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更新日期:1989-04-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.43.4.835-839.1982
更新日期:1982-04-01 00:00:00
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journal_title:Applied and environmental microbiology
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更新日期:1991-09-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.40.3.678-680.1980
更新日期:1980-09-01 00:00:00