Inhibition of Clostridium botulinum by antioxidants, phenols, and related compounds.

Abstract:

:A total of 75 compounds, including antioxidants, preservatives, gallic acid and p-hydroxybenzoic acid esters, hydroquinones, hydroxyquinolines, phenol derivatives, and related compounds, were screened for their antibotulinal activity in prereduced Thiotone-yeast extract-glucose broth. The most effective inhibitors of Clostridium botulinum growth and toxin production were long-chain esters of p-hydroxybenzoic acid and gallic acid, antioxidants, and butylphenol derivatives. The antioxidant nordihydroguaiaretic acid at 100 microgram/ml delayed the growth and toxin production for the entire incubation period (7 days). Other antioxidants, such as butylated hydroxytoluene, butylated hydroxyanisole, and tert-butylhydroquinone were also very effective (at 200 to 400 microgram/ml) for the inhibition of C. botulinum growth and toxin production. Toxin was detected, although no detectable growth was found by daily absorbance measurements, in the prereduced medium containing 50 to 400 microgram of 8-hydroxyquinoline, pentylphenol, tert-pentylphenol, 3,5-ditert-butylphenol, 3,5-ditert-butylcatechol, (2-hydroxydiphenyl)methane, or (4-hydroxydiphenyl)methane per ml.

journal_name

Appl Environ Microbiol

authors

Reddy NR,Pierson MD,Lechowich RV

doi

10.1128/AEM.43.4.835-839.1982

subject

Has Abstract

pub_date

1982-04-01 00:00:00

pages

835-9

issue

4

eissn

0099-2240

issn

1098-5336

journal_volume

43

pub_type

杂志文章
  • Demethylation of Veratrole by Cytochrome P-450 in Streptomyces setonii.

    abstract::The actinomycete Streptomyces setonii 75Vi2 demethylates vanillic acid and guaiacol to protocatechuic acid and catechol, respectively, and then metabolizes the products by the beta-ketoadipate pathway. UV spectroscopy showed that this strain could also metabolize veratrole (1,2-dimethoxybenzene). When grown in veratro...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.52.1.98-100.1986

    authors: Sutherland JB

    更新日期:1986-07-01 00:00:00

  • A PCR assay To discriminate human and ruminant feces on the basis of host differences in Bacteroides-Prevotella genes encoding 16S rRNA.

    abstract::Our purpose was to develop a rapid, inexpensive method of diagnosing the source of fecal pollution in water. In previous research, we identified Bacteroides-Prevotella ribosomal DNA (rDNA) PCR markers based on analysis. These markers length heterogeneity PCR and terminal restriction fragment length polymorphism distin...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.66.10.4571-4574.2000

    authors: Bernhard AE,Field KG

    更新日期:2000-10-01 00:00:00

  • Denitrification, dissimilatory reduction of nitrate to ammonium, and nitrification in a bioturbated estuarine sediment as measured with N and microsensor techniques.

    abstract::Nitrogen and oxygen transformations were studied in a bioturbated (reworked by animals) estuarine sediment (Norsminde Fjord, Denmark) by using a combination of N isotope (NO(3)), specific inhibitor (C(2)H(2)), and microsensor (N(2)O and O(2)) techniques in a continuous-flow core system. The estuarine water was NO(3) r...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.58.1.303-313.1992

    authors: Binnerup SJ,Jensen K,Revsbech NP,Jensen MH,Sørensen J

    更新日期:1992-01-01 00:00:00

  • Fitness of Salmonella enterica serovar Thompson in the cilantro phyllosphere.

    abstract::The epiphytic fitness of Salmonella enterica was assessed on cilantro plants by using a strain of S. enterica serovar Thompson that was linked to an outbreak resulting from cilantro. Salmonella serovar Thompson had the ability to colonize the surface of cilantro leaves, where it was detected by confocal laser scanning...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.68.7.3614-3621.2002

    authors: Brandl MT,Mandrell RE

    更新日期:2002-07-01 00:00:00

  • Intracellular accumulation of polyphosphate by the yeast Candida humicola G-1 in response to acid pH.

    abstract::Cells of a newly isolated environmental strain of Candida humicola accumulated 10-fold more polyphosphate (polyP), during active growth, when grown in complete glucose-mineral salts medium at pH 5.5 than when grown at pH 7.5. Neither phosphate starvation, nutrient limitation, nor anaerobiosis was required to induce po...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.66.9.4068-4073.2000

    authors: McGrath JW,Quinn JP

    更新日期:2000-09-01 00:00:00

  • A convenient and robust in vivo reporter system to monitor gene expression in the human pathogen Helicobacter pylori.

    abstract::Thirty years of intensive research have significantly contributed to our understanding of Helicobacter pylori biology and pathogenesis. However, the lack of convenient genetic tools, in particular the limited effectiveness of available reporter systems, has notably limited the toolbox for fundamental and applied studi...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01252-12

    authors: Vannini A,Agriesti F,Mosca F,Roncarati D,Scarlato V,Danielli A

    更新日期:2012-09-01 00:00:00

  • Lyophilization of rumen fluid for use in culture media.

    abstract::The supernatant from centrifugation at 1,000 x g of strained rumen fluid was lyophilized, and the residue and sublimate fractions were used to replace fresh rumen fluid in a complete roll tube medium for enumeration of total rumen bacteria. Most of the growth-supporting nutrients in fresh rumen fluid were found in the...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.55.12.3237-3239.1989

    authors: Dehority BA,Tirabasso PA

    更新日期:1989-12-01 00:00:00

  • Identification of a Novel Elastin-Degrading Enzyme from the Fish Pathogen Flavobacterium psychrophilum.

    abstract::Hydrolytic extracellular enzymes degrading host tissues potentially play a role in bacterial pathogenesis. Flavobacterium psychrophilum is an important bacterial pathogen of salmonid fish reared in freshwater throughout the world. Diversity among isolates has been described at the phenotypic, serological, and genomic ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02535-18

    authors: Rochat T,Pérez-Pascual D,Nilsen H,Carpentier M,Bridel S,Bernardet JF,Duchaud E

    更新日期:2019-03-06 00:00:00

  • Genome-wide gene expression patterns and growth requirements suggest that Pelobacter carbinolicus reduces Fe(III) indirectly via sulfide production.

    abstract::Although Pelobacter species are closely related to Geobacter species, recent studies suggested that Pelobacter carbinolicus may reduce Fe(III) via a different mechanism because it lacks the outer-surface c-type cytochromes that are required for Fe(III) reduction by Geobacter sulfurreducens. Investigation into the mech...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02901-07

    authors: Haveman SA,DiDonato RJ Jr,Villanueva L,Shelobolina ES,Postier BL,Xu B,Liu A,Lovley DR

    更新日期:2008-07-01 00:00:00

  • Chloroethene biodegradation in sediments at 4 degrees C.

    abstract::Microbial reductive dechlorination of [1,2-14C]trichloroethene to [14C]cis-dichloroethene and [14C]vinyl chloride was observed at 4 degrees C in anoxic microcosms prepared with cold temperature-adapted aquifer and river sediments from Alaska. Microbial anaerobic oxidation of [1,2-14C]cis-dichloroethene and [1,2-14C]vi...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.71.10.6414-6417.2005

    authors: Bradley PM,Richmond S,Chapelle FH

    更新日期:2005-10-01 00:00:00

  • Isolation and Characterization of Marine Caulobacters and Assessment of Their Potential for Genetic Experimentation.

    abstract::A total of 25 marine caulobacters were isolated from littoral marine sources. Several aspects of their physiology and morphology were examined, as well as their suitability for genetic manipulation in laboratory cultivation. Caulobacters were readily isolated from all sources, including samples from areas containing p...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.54.3.809-817.1988

    authors: Anast N,Smit J

    更新日期:1988-03-01 00:00:00

  • Long-chain aldehyde dehydrogenase that participates in n-alkane utilization and wax ester synthesis in Acinetobacter sp. strain M-1.

    abstract::A long-chain aldehyde dehydrogenase, Ald1, was found in a soluble fraction of Acinetobacter sp. strain M-1 cells grown on n-hexadecane as a sole carbon source. The gene (ald1) was cloned from the chromosomal DNA of the bacterium. The open reading frame of ald1 was 1,512 bp long, corresponding to a protein of 503 amino...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.66.8.3481-3486.2000

    authors: Ishige T,Tani A,Sakai Y,Kato N

    更新日期:2000-08-01 00:00:00

  • Interactions between the diatom Thallasiosira pseudonanna and an associated pseudomonad in a mariculture system.

    abstract::The marine diatom Thallasiosira pseudonanna (3H) and several bacteria associated with it were isolated from batch cultures at the University of Delaware mariculture facility. The interaction between the algae and each of the bacteria was investigated. One of the isolates, T827/2B (Pseudomonas sp.), was incapable of su...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.35.4.791-796.1978

    authors: Baker KH,Herson DS

    更新日期:1978-04-01 00:00:00

  • 7 alpha-Dehydroxylation of bile acids by resting cells of a Eubacterium lentum-like intestinal anaerobe, strain c-25.

    abstract::7 alpha-Dehydroxylation of cholic acid and chenodeoxycholic acid by whole cells of strain c-25, a Eubacterium lentum-like intestinal anaerobe, was studied. 7 alpha-Dehydroxylase activity was observed only in whole cells grown in the presence of the primary bile acid (cholic acid or chenodeoxycholic acid). Chenodeoxych...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.47.4.735-739.1984

    authors: Masuda N,Oda H,Hirano S,Masuda M,Tanaka H

    更新日期:1984-04-01 00:00:00

  • Phenolic Amides Are Potent Inhibitors of De Novo Nucleotide Biosynthesis.

    abstract::An outstanding challenge toward efficient production of biofuels and value-added chemicals from plant biomass is the impact that lignocellulose-derived inhibitors have on microbial fermentations. Elucidating the mechanisms that underlie their toxicity is critical for developing strategies to overcome them. Here, using...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01324-15

    authors: Pisithkul T,Jacobson TB,O'Brien TJ,Stevenson DM,Amador-Noguez D

    更新日期:2015-09-01 00:00:00

  • Multivariate logistic regression for predicting total culturable virus presence at the intake of a potable-water treatment plant: novel application of the atypical coliform/total coliform ratio.

    abstract::Predicting the presence of enteric viruses in surface waters is a complex modeling problem. Multiple water quality parameters that indicate the presence of human fecal material, the load of fecal material, and the amount of time fecal material has been in the environment are needed. This paper presents the results of ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02780-06

    authors: Black LE,Brion GM,Freitas SJ

    更新日期:2007-06-01 00:00:00

  • Efficient production of polymyxin in the surrogate host Bacillus subtilis by introducing a foreign ectB gene and disrupting the abrB gene.

    abstract::In our previous study, Bacillus subtilis strain BSK3S, containing a polymyxin biosynthetic gene cluster from Paenibacillus polymyxa, could produce polymyxin only in the presence of exogenously added L-2,4-diaminobutyric acid (Dab). The dependence of polymyxin production on exogenous Dab was removed by introducing an e...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.07912-11

    authors: Park SY,Choi SK,Kim J,Oh TK,Park SH

    更新日期:2012-06-01 00:00:00

  • Microbial community shifts reflect losses of native soil carbon with pyrogenic and fresh organic matter additions and are greatest in low-carbon soils.

    abstract::Soil organic carbon (SOC) plays an important role in regulating global climate change, carbon and nutrient cycling in soils, and soil moisture. Organic matter (OM) additions to soils can affect the rate at which SOC is mineralized by microbes, with potentially important effects on SOC stocks. Understanding how pyrogen...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02555-20

    authors: Whitman T,DeCiucies S,Hanley K,Enders A,Woolet J,Lehmann J

    更新日期:2021-01-29 00:00:00

  • Characterization of predominant bacteria from the colons of normal and dysenteric pigs.

    abstract::Bacterial populations adherent to the mucosa of the proximal colons of weaned, healthy pigs were compared with populations from pigs with dysentery induced by inoculation with a culture of Treponema hyodysenteriae. Isolates (136) representative of the predominant flora adherent to colonic epithelia of normal pigs and ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.48.5.964-969.1984

    authors: Robinson IM,Whipp SC,Bucklin JA,Allison MJ

    更新日期:1984-11-01 00:00:00

  • Efficient production of artificially designed gelatins with a Bacillus brevis system.

    abstract::Artificially designed gelatins comprising tandemly repeated 30-amino-acid peptide units derived from human alphaI collagen were successfully produced with a Bacillus brevis system. The DNA encoding the peptide unit was synthesized by taking into consideration the codon usage of the host cells, but no clones having a t...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.66.1.304-309.2000

    authors: Kajino T,Takahashi H,Hirai M,Yamada Y

    更新日期:2000-01-01 00:00:00

  • Factors Affecting the Germination of Akinetes of Nodularia spumigena (Cyanobacteriaceae).

    abstract::Nutritional and physical factors which influence the germination of akinetes of Nodularia spumigena (Cyanobacteriaceae) were examined. Low concentrations of phosphorus (<0.9 muM) were required for germination. Nitrate had no effect, but ammonia, at concentrations of >45 muM, inhibited germination. Salinities of >20 pe...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.49.1.73-78.1985

    authors: Huber AL

    更新日期:1985-01-01 00:00:00

  • Steady-State Effects of 2,5,2',5'-Tetrachlorobiphenyl on Growth, Photosynthesis, and P Uptake in Selenastrum capricornutum.

    abstract::The steady-state effect of 2,5,2',5'-tetrachlorobiphenyl (TCBP) on the green alga Selenastrum capricornutum was investigated in a P-limited two-stage chemostat system. The partition coefficient of this polychlorinated biphenyl congener was 5.9 x 10 in steady-state cultures. At a cellular TCBP concentration of 12.2 x 1...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.54.6.1394-1398.1988

    authors: Rhee GY,Shane L,Denucci A

    更新日期:1988-06-01 00:00:00

  • Rapid mineralization of the phenylurea herbicide diuron by Variovorax sp. strain SRS16 in pure culture and within a two-member consortium.

    abstract::The phenylurea herbicide diuron [N-(3,4-dichlorophenyl)-N,N-dimethylurea] is widely used in a broad range of herbicide formulations, and consequently, it is frequently detected as a major water contaminant in areas where there is extensive use. We constructed a linuron [N-(3,4-dichlorophenyl)-N-methoxy-N-methylurea]- ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02687-07

    authors: Sørensen SR,Albers CN,Aamand J

    更新日期:2008-04-01 00:00:00

  • Oxygen-sensing reporter strain of Pseudomonas fluorescens for monitoring the distribution of low-oxygen habitats in soil.

    abstract::The root-colonizing bacterium Pseudomonas fluorescens CHA0 was used to construct an oxygen-responsive biosensor. An anaerobically inducible promoter of Pseudomonas aeruginosa, which depends on the FNR (fumarate and nitrate reductase regulation)-like transcriptional regulator ANR (anaerobic regulation of arginine deimi...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.65.9.4085-4093.1999

    authors: Højberg O,Schnider U,Winteler HV,Sørensen J,Haas D

    更新日期:1999-09-01 00:00:00

  • Cloning of novel enterotoxin genes from Bacillus cereus and Bacillus thuringiensis.

    abstract::A novel enterotoxin gene was cloned from Bacillus cereus FM1, and its nucleotide sequence was determined. Previously, a 45-kDa protein causing characteristic enterotoxin symptoms in higher animals had been isolated (K. Shinagawa, p. 181-193, in A. E. Pohland et al., ed., Microbial Toxins in Foods and Feeds, 1990) from...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.63.3.1054-1057.1997

    authors: Asano SI,Nukumizu Y,Bando H,Iizuka T,Yamamoto T

    更新日期:1997-03-01 00:00:00

  • Mechanisms of Mineral Substrate Acquisition in a Thermoacidophile.

    abstract::The thermoacidophile Acidianus is widely distributed in Yellowstone National Park hot springs that span large gradients in pH (1.60 to 4.84), temperature (42 to 90°C), and mineralogical composition. To characterize the potential role of flexibility in mineral-dependent energy metabolism in contributing to the widespre...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00334-18

    authors: Amenabar MJ,Boyd ES

    更新日期:2018-05-31 00:00:00

  • Weak activity of haloalkane dehalogenase LinB with 1,2,3-trichloropropane revealed by X-Ray crystallography and microcalorimetry.

    abstract::1,2,3-Trichloropropane (TCP) is a highly toxic and recalcitrant compound. Haloalkane dehalogenases are bacterial enzymes that catalyze the cleavage of a carbon-halogen bond in a wide range of organic halogenated compounds. Haloalkane dehalogenase LinB from Sphingobium japonicum UT26 has, for a long time, been consider...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02416-06

    authors: Monincová M,Prokop Z,Vévodová J,Nagata Y,Damborsky J

    更新日期:2007-03-01 00:00:00

  • Inoculant Production with Diluted Liquid Cultures of Rhizobium spp. and Autoclaved Peat: Evaluation of Diluents, Rhizobium spp., Peats, Sterility Requirements, Storage, and Plant Effectiveness.

    abstract::Fully grown broth cultures of various fast- and slow-growing rhizobia were deliberately diluted with various diluents before their aseptic incorporation into autoclaved peat in polypropylene bags (aseptic method) or mixed with the peat autoclaved in trays (tray method). In a factorial experiment with the aseptic metho...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.50.2.398-405.1985

    authors: Somasegaran P

    更新日期:1985-08-01 00:00:00

  • Helix alpha 4 of the Bacillus thuringiensis Cry1Aa toxin plays a critical role in the postbinding steps of pore formation.

    abstract::Helix alpha 4 of Bacillus thuringiensis Cry toxins is thought to play a critical role in the toxins' mode of action. Accordingly, single-site substitutions of many Cry1Aa helix alpha 4 amino acid residues have previously been shown to cause substantial reductions in the protein's pore-forming activity. Changes in prot...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01930-08

    authors: Girard F,Vachon V,Préfontaine G,Marceau L,Schwartz JL,Masson L,Laprade R

    更新日期:2009-01-01 00:00:00

  • Deformed wing virus implicated in overwintering honeybee colony losses.

    abstract::The worldwide decline in honeybee colonies during the past 50 years has often been linked to the spread of the parasitic mite Varroa destructor and its interaction with certain honeybee viruses. Recently in the United States, dramatic honeybee losses (colony collapse disorder) have been reported; however, there remain...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02227-09

    authors: Highfield AC,El Nagar A,Mackinder LC,Noël LM,Hall MJ,Martin SJ,Schroeder DC

    更新日期:2009-11-01 00:00:00