Abstract:
:Bacterial populations adherent to the mucosa of the proximal colons of weaned, healthy pigs were compared with populations from pigs with dysentery induced by inoculation with a culture of Treponema hyodysenteriae. Isolates (136) representative of the predominant flora adherent to colonic epithelia of normal pigs and isolates (162) from pigs with dysentery were cultured anaerobically on a rumen fluid-based medium and characterized. Most (71%) of the isolates from colonic epithelia of normal pigs were gram positive, whereas 88% of the epithelia-associated isolates from pigs with dysentery were gram negative. The geometric mean of colony counts was 5.7 X 10(7)/cm2 of colonic tissue from three normal pigs and 7.7 X 10(8)/cm2 from four pigs with dysentery. A number of isolates obtained from contents of the lumens of normal pigs with dysentery were also characterized. Comparison of isolates from epithelial tissue and from contents of the lumens of the same pig indicated that these populations were different. Our results indicate that physiological changes that occur in the colons of pigs with dysentery are accompanied by marked changes in the microbial populations in the colons. The factors which regulate the population changes are not yet understood.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Robinson IM,Whipp SC,Bucklin JA,Allison MJdoi
10.1128/AEM.48.5.964-969.1984subject
Has Abstractpub_date
1984-11-01 00:00:00pages
964-9issue
5eissn
0099-2240issn
1098-5336journal_volume
48pub_type
杂志文章abstract::Rhodococcus erythropolis I-19, containing multiple copies of key dsz genes, was used to desulfurize alkylated dibenzothiophenes (Cx-DBTs) found in a hydrodesulfurized middle-distillate petroleum (MD 1850). Initial desulfurization rates of dibenzothiophene (DBT) and MD 1850 by I-19 were 5.0 and 2.5 micromol g dry cell ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.65.11.4967-4972.1999
更新日期:1999-11-01 00:00:00
abstract::Two yellow pigments were isolated for the first time from the entomopathogenic bacterium Photorhabdus luminescens in liquid culture and were identified as the anthraquinone derivatives 3,8-dimethoxy-1-hydroxy-9,10-anthraquinone (minor) and 1,3-dimethoxy-8-hydroxy-9,10-anthraquinone (major). A known antibiotic, 3,5-dih...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.61.12.4329-4333.1995
更新日期:1995-12-01 00:00:00
abstract::Epilithic periphyton communities were sampled at three sites on the Minnesota shoreline of Lake Superior from June 2004 to August 2005 to determine if fecal coliforms and Escherichia coli were present throughout the ice-free season. Fecal coliform densities increased up to 4 orders of magnitude in early summer, reache...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02654-06
更新日期:2007-06-01 00:00:00
abstract::In this study, we examine bacterial attachment and survival on a titanium (Ti) cathode coated with various carbon nanomaterials (CNM): pristine carbon nanotubes (CNT), oxidized carbon nanotubes (O-CNT), oxidized-annealed carbon nanotubes (OA-CNT), carbon black (CB), and reduced graphene oxide (rGO). The carbon nanomat...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00582-15
更新日期:2015-07-01 00:00:00
abstract::Biofilms are composed of bacterial cells encased in a self-synthesized, extracellular polymeric matrix. Poly-beta(1,6)-N-acetyl-d-glucosamine (PNAG) is a major biofilm matrix component in phylogenetically diverse bacteria. In this study we investigated the physical and chemical properties of the PNAG matrix in biofilm...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01900-08
更新日期:2009-03-01 00:00:00
abstract::Salmonella spp. are remarkably adaptable pathogens, and this adaptability allows these bacteria to thrive in a variety of environments and hosts. The mechanisms with which these pathogens establish within a niche amid the native microbiota remain poorly understood. Here, we aimed to uncover the mechanisms that enable ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01913-17
更新日期:2018-02-14 00:00:00
abstract::Ten phenols were selected as natural laccase mediators after screening 44 different compounds with a recalcitrant dye (Reactive Black 5) as a substrate. Their performances were evaluated at different mediator/dye ratios and incubation times (up to 6 h) by the use of Pycnoporus cinnabarinus and Trametes villosa laccase...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.71.4.1775-1784.2005
更新日期:2005-04-01 00:00:00
abstract::The comparative adherence of seven strains of Staphylococcus epidermidis to plastic surfaces was measured by an agar overlay technique in which adherent organisms were counted by their ability to form colonies under an agar overlay. The degree of adherence to plastics decreased in the order polystyrene, polyvinyl chlo...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.50.5.1322-1324.1985
更新日期:1985-11-01 00:00:00
abstract::Production of bacteriocin activity designated pediocin PA-1 was associated with the presence of a 6.2-megadalton plasmid in Pediococcus acidilactici PAC1.0. The bacteriocin exhibited activity against P. acidilactici, P. pentosaceus, Lactobacillus plantarum, L. casei, L. bifermentans, and Leuconostoc mesenteroides subs...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.53.10.2534-2538.1987
更新日期:1987-10-01 00:00:00
abstract::A novel agar medium, chromogenic Salmonella esterase (CSE) agar, for the differentiation of salmonellae is described. The agar contains peptones and nutrient extracts together with the following (grams per liter unless otherwise specified): 4-[2-(4-octanoyloxy-3, 5-dimethoxyphenyl)-vinyl]-quinolinium-1-(propan-3-yl ca...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.65.2.807-812.1999
更新日期:1999-02-01 00:00:00
abstract::A methylomonad culture was isolated from pond water and examined as a potential source of single-cell protein. A medium containing magnesium sulfate, ammonium hydroxide, sodium phosphate, tap water, and methanol supported the growth of the isolate. Optimal growth conditions in batch cultures for the organism were: tem...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.33.2.269-274.1977
更新日期:1977-02-01 00:00:00
abstract::Campylobacter jejuni is a pathogenic, microaerophilic, gram-negative, mesophilic bacterium. Three strains isolated from humans with enteric campylobacteriosis were able to survive at high population levels (10(7) cells ml-1) as viable-but-nonculturable (VBNC) forms in microcosm water. The VBNC forms of the three C. je...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.65.3.1110-1116.1999
更新日期:1999-03-01 00:00:00
abstract::A database was probed with artificial neural network (ANN) and multivariate logistic regression (MLR) models to investigate the efficacy of predicting PCR-identified human adenovirus (ADV), Norwalk-like virus (NLV), and enterovirus (EV) presence or absence in shellfish harvested from diverse countries in Europe (Spain...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.71.9.5244-5253.2005
更新日期:2005-09-01 00:00:00
abstract::Bacillus subtilis RB14, which showed antibiotic activities against several phytopathogens in vitro by producing the antibiotics iturin A and surfactin, was subjected to a pot test to investigate its ability to suppress damping-off of tomato seedlings caused by Rhizoctonia solani. To facilitate recovery from soil, B. s...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.62.11.4081-4085.1996
更新日期:1996-11-01 00:00:00
abstract::In the vast number of random mutagenesis experiments that have targeted protein thermostability, single amino acid substitutions that increase the apparent melting temperature (Tm) of the enzyme more than 1 to 2 degrees C are rare and often require the creation of a large library of mutated genes. Here we present a ca...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02220-07
更新日期:2008-02-01 00:00:00
abstract:UNLABELLED:Endosymbiosis is a unique form of interaction between organisms, with one organism dwelling inside the other. One of the most widespread endosymbionts is Wolbachia pipientis, a maternally transmitted bacterium carried by insects, crustaceans, mites, and filarial nematodes. Although candidate proteins that co...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01255-16
更新日期:2016-08-15 00:00:00
abstract::The Sinorhizobium meliloti ORFeome project cloned 6,314 open reading frames (ORFs) into a modified Gateway entry vector system from which the ORFs could be transferred to destination vectors in vivo via bacterial conjugation. In this work, a reporter gene destination vector, pMK2030, was constructed and used to genera...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01468-08
更新日期:2008-11-01 00:00:00
abstract::Addition of 2 mg of Pb2+/g of soil concident with or after amendment with starch or maltose resulted in 75 and 50% decreases in net synthesis of amylase and alpha-glucosidase, respectively. Invertase synthesis in sucrose-amended soil was transiently reduced after Pb2+ addition. Amylase activity was several times less ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.33.2.262-268.1977
更新日期:1977-02-01 00:00:00
abstract::For PCR detection of Coxiella burnetii in various clinical specimens we developed a sample preparation method in which silica binding of DNA was used. This method was found to be fast, easily performed with large numbers of samples, and equally sensitive for all of the specimens tested (livers, spleens, placentas, hea...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.64.11.4234-4237.1998
更新日期:1998-11-01 00:00:00
abstract::Renibacterium salmoninarum, a gram-positive diplococcobacillus, causes bacterial kidney disease, a condition that can result in extensive morbidity and mortality among stocks of fish. An immunodominant extracellular protein, called major soluble antigen (MSA), is encoded by two identical genes, msa1 and msa2. We found...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.70.11.6488-6494.2004
更新日期:2004-11-01 00:00:00
abstract::Four soil temperature and moisture treatment regimens were imposed on Florunner peanuts 94 days after planting in experimental plots in 1980. At harvest (145 days after planting), the incidence of the Aspergillus flavus group and the aflatoxin concentration were greatest in damaged kernels. Extensive colonization of s...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.45.2.628-633.1983
更新日期:1983-02-01 00:00:00
abstract::The use of Lactococcus lactis (the most extensively characterized lactic acid bacterium) as a delivery organism for heterologous proteins is, in some cases, limited by low production levels and poor-quality products due to surface proteolysis. In this study, we combined in one L. lactis strain use of the nisin-inducib...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.68.6.3141-3146.2002
更新日期:2002-06-01 00:00:00
abstract::A novel strain of Halomonas (Tc-202), which has the capability of removing Tc(VII) from solid- and aqueous-phase material aerobically, was isolated from the marine environment. Tc-202 removed 55% of the total 99Tc in solutions at 15 degrees C by reducing Tc(VII) to Tc(V), but other Halomonas strains did not. ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00819-06
更新日期:2006-12-01 00:00:00
abstract::Enrichment was performed to isolate organisms that could utilize reduced phosphorus compounds as their sole phosphorus sources. One isolate that grew well with either hypophosphite or phosphite was identified by 16S rRNA gene analysis as a strain of Alcaligenes faecalis. The genes required for oxidation of hypophosphi...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.71.1.290-296.2005
更新日期:2005-01-01 00:00:00
abstract::We describe here the development of a single-reaction multiplex PCR assay for the enterotoxin genes from Staphylococcus aureus that utilizes a universal toxin gene primer in combination with toxin-specific primers to amplify characteristic toxin gene products. In combination with a new DNA purification method, the ass...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.66.4.1347-1353.2000
更新日期:2000-04-01 00:00:00
abstract::This report describes the use of an oligonucleotide macroarray to profile the expression of 375 genes in Lactococcus lactis subsp. lactis IL1403 during heat, acid, and osmotic stress. A set of known stress-associated genes in IL1403 was used as the internal control on the array. Every stress response was accurately de...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.70.11.6738-6747.2004
更新日期:2004-11-01 00:00:00
abstract::Colonization of livestock with bacteria resistant to antibiotics is considered a risk for the entry of drug-resistant pathogens into the food chain. For this reason, there is a need for novel concepts to address the eradication of drug-resistant commensals on farms. In the present report, we evaluated the decontaminat...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01713-15
更新日期:2015-11-01 00:00:00
abstract::The interactions of bacteria isolated from corroded copper coupons on thin films of copper evaporated onto germanium internal reflection elements were evaluated nondestructively in real time by attentuated total reflectance Fourier transform infrared spectroscopy. The films were stable in the presence of flowing or st...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.57.7.1956-1962.1991
更新日期:1991-07-01 00:00:00
abstract::Listeria monocytogenes can change its cellular morphology from bacilli to cocci during the transition to the long-term-survival (LTS) phase. The LTS cells demonstrated increased baro- and thermotolerance compared to their vegetative counterparts. So far, the underlying mechanisms that trigger this morphological and ph...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00596-11
更新日期:2011-09-01 00:00:00
abstract::The microaerophilic nature of Campylobacter species implies an inherent sensitivity towards oxygen and its reduction products, particularly the superoxide anion. The deleterious effects of exposure to superoxide radicals are counteracted by the activity of superoxide dismutase (SOD). We have shown previously that Camp...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.65.6.2540-2546.1999
更新日期:1999-06-01 00:00:00