Agar overlay method to measure adherence of Staphylococcus epidermidis to four plastic surfaces.

abstract：:Ten laboratories participated in an interlaboratory comparison of determination of bioleaching rates of a pyrite reference material. A standardized procedure and a single strain of Thiobacillus ferrooxidans were used in this study. The mean rate of bioleaching of the pyrite reference material was 12.4 mg of Fe per lit...

journal_title：Applied and environmental microbiology

authors： Olson GJ

更新日期：1991-03-01 00:00:00

abstract：:Selectively regulating gene expression is an essential molecular tool that is lacking for many pathogenic gram-positive bacteria. In this report, we describe the evaluation of a series of promoters regulated by the bacteriophage P1 temperature-sensitive C1 repressor in Enterococcus faecium, Enterococcus faecalis, and ...

journal_title：Applied and environmental microbiology

authors： Schofield DA,Westwater C,Hoel BD,Werner PA,Norris JS,Schmidt MG

更新日期：2003-06-01 00:00:00

abstract：:A highly specific medium was developed for the enumeration of lactate-utilizing bacteria in the rumen of sheep. This medium, which contained 2.0% lactate, 2.0% Trypticase, 0.2% yeast extract, and volatile fatty acids, hemin, and trace elements in place of rumen fluid, enabled high counts (42 x 10 to 190 x 10/g of inge...

journal_title：Applied and environmental microbiology

authors： Mackie RI,Heath S

更新日期：1979-09-01 00:00:00

abstract：:Information on the interaction between mixed populations in the rumen and plant phenolics is required to fully elucidate the limitations of phenolic compounds on forage digestibility. The objective of this study was to examine the degradation of Italian ryegrass (Lolium multiflorum L.) hay incubated with mixed ruminal...

journal_title：Applied and environmental microbiology

authors： Theodorou MK,Gascoyne DJ,Akin DE,Hartley RD

更新日期：1987-05-01 00:00:00

abstract：:The kinetics of oxygen reduction by Desulfovibrio salexigens Mast1 and the role of polyglucose in this activity were examined and compared with those of strains of D. desulfuricans and D. gigas. Oxidation rates were highest at air saturation (up to 40 nmol of O(2) min mg of protein) and declined with decreasing oxygen...

journal_title：Applied and environmental microbiology

authors： van Niel EW,Gottschal JC

更新日期：1998-03-01 00:00:00

abstract：:This work presents the characterization of an uptake hydrogenase from a marine filamentous nonheterocystous cyanobacterium, Lyngbya majuscula CCAP 1446/4. The structural genes encoding the uptake hydrogenase (hupSL) were isolated and characterized, and regulatory sequences were identified upstream of hupS. In silico a...

journal_title：Applied and environmental microbiology

authors： Leitão E,Oxelfelt F,Oliveira P,Moradas-Ferreira P,Tamagnini P

更新日期：2005-08-01 00:00:00

abstract：:Pseudomonas fluorescens 2-79 suppresses take-all, a major root disease of wheat caused by Gaeumannomyces graminis var. tritici. The bacteria produce an antibiotic, phenazine-1-carboxylic acid (PCA), and a fluorescent pyoverdin siderophore. Previous studies have established that PCA has an important role in the biologi...

journal_title：Applied and environmental microbiology

authors： Hamdan H,Weller DM,Thomashow LS

更新日期：1991-11-01 00:00:00

abstract：:Cells in desiccated Salmonella enterica serovar Typhimurium rdar (red, dry, and rough) morphotype colonies were examined for culturability and infectivity after 30 months. Culturability decreased only 10-fold; however, cells were underrepresented on Salmonella selective media containing bile salts. These cells were mi...

journal_title：Applied and environmental microbiology

authors： Apel D,White AP,Grassl GA,Finlay BB,Surette MG

更新日期：2009-07-01 00:00:00

abstract：:Because Bacteroides spp. are obligate anaerobes that dominate the human fecal flora, and because some species may live only in the human intestine, these bacteria might be useful to distinguish human from nonhuman sources of fecal pollution. To test this hypothesis, PCR primers specific for 16S rRNA gene sequences of ...

journal_title：Applied and environmental microbiology

authors： Kreader CA

更新日期：1995-04-01 00:00:00

abstract：:SslI, a type II restriction endonuclease, was purified from Streptococcus salivarius subsp. thermophilus strain BSN 45. SslI is an isoschizomer of BstNI. SslI activity was maximum at pH 8.8, 0 to 50 mM NaCl, 2 to 8 mM Mg2+, and 42 degrees C. Activity against phage DNA in vitro was demonstrated. ...

journal_title：Applied and environmental microbiology

authors： Benbadis L,Garel JR,Hartley DL

更新日期：1991-12-01 00:00:00

abstract：:The conversion of ochratoxin C to ochratoxin A was studied in rats after oral and intravenous administration. The concentration of ochratoxin A in the blood as a function of time was the same after oral administration of equivalent amounts of either ochratoxin C or ochratoxin A. The maximum ochratoxin A concentrations...

journal_title：Applied and environmental microbiology

authors： Fuchs R,Hult K,Peraica M,Radić B,Plestina R

更新日期：1984-07-01 00:00:00

abstract：:Populations of feral pigs (Sus scrofa) may serve as an environmental reservoir of Cryptosporidium parvum oocysts and Giardia sp. cysts for source water. We conducted a cross-sectional study to determine the prevalence of and associated demographic and environmental risk factors for the shedding of C. parvum oocysts an...

journal_title：Applied and environmental microbiology

authors： Atwill ER,Sweitzer RA,Pereira MG,Gardner IA,Van Vuren D,Boyce WM

更新日期：1997-10-01 00:00:00

abstract：:A novel display system is described that allows highly efficient immobilization of heterologous proteins on bacterial surfaces in applications for which the use of genetically modified bacteria is less desirable. This system is based on nonliving and non-genetically modified gram-positive bacterial cells, designated g...

journal_title：Applied and environmental microbiology

authors： Bosma T,Kanninga R,Neef J,Audouy SA,van Roosmalen ML,Steen A,Buist G,Kok J,Kuipers OP,Robillard G,Leenhouts K

更新日期：2006-01-01 00:00:00

abstract：:Cyanuric acid is an industrial chemical produced during the biodegradation of s-triazine pesticides. The biodegradation of cyanuric acid has been elucidated using a single model system, Pseudomonas sp. strain ADP, in which cyanuric acid hydrolase (AtzD) opens the s-triazine ring and AtzEG deaminates the ring-opened pr...

journal_title：Applied and environmental microbiology

authors： Aukema KG,Tassoulas LJ,Robinson SL,Konopatski JF,Bygd MD,Wackett LP

更新日期：2020-01-07 00:00:00

abstract：:A method is described whereby the presence of Salmonella spp. can be detected within 8 to 24 h of sample collection. The method depends upon the interaction of Salmonella spp. with the Salmonella-specific Felix-O1 bacteriophage. This interaction results in an increase in concentration of the bacteriophage which is det...

journal_title：Applied and environmental microbiology

authors： Hirsh DC,Martin LD

更新日期：1983-01-01 00:00:00

abstract：:The noncanonical alcohol dehydrogenase AlkJ is encoded on the alkane-metabolizing alk operon of the mesophilic bacterium Pseudomonas putida GPo1. To gain insight into the enzymology of AlkJ, we have produced the recombinant protein in Escherichia coli and purified it to homogeneity using His6 tag affinity and size exc...

journal_title：Applied and environmental microbiology

authors： Kirmair L,Skerra A

更新日期：2014-04-01 00:00:00

abstract：:An improved method of dot-blot hybridization to detect hepatitis A virus (HAV) was developed with single-stranded RNA (ssRNA) probes. Radioactive and nonradioactive ssRNA probes were generated by in vitro transcription of HAV templates inserted into the plasmid pGEM-1. 32P-labeled ssRNA probes were at least eightfold ...

journal_title：Applied and environmental microbiology

authors： Jiang X,Estes MK,Metcalf TG

更新日期：1987-10-01 00:00:00

abstract：:The virulence factor internalin A (InlA) facilitates the uptake of Listeria monocytogenes by epithelial cells that express the human isoform of E-cadherin. Previous studies identified naturally occurring premature stop codon (PMSC) mutations in inlA and demonstrated that these mutations are responsible for virulence a...

journal_title：Applied and environmental microbiology

authors： Van Stelten A,Nightingale KK

更新日期：2008-12-01 00:00:00

abstract：:Strains of Ascochyta rabiei which are pathogenic to chickpea (Cicer arietinum L.) readily catabolized the main chickpea isoflavone biochanin A (5,7-dihydroxy-4'-methoxyisoflavone). 3'-Hydroxylation and O-demethylation reactions led to the isoflavones pratensein, genistein, and orobol, which were rapidly further degrad...

journal_title：Applied and environmental microbiology

authors： Kraft B,Barz W

更新日期：1985-07-01 00:00:00

abstract：:The biological succession that occurs during the first year of life in the gut of infants in Western countries is broadly predictable in terms of the increasing complexity of the composition of microbiotas. Less information is available about microbiotas in Asian countries, where environmental, nutritional, and cultur...

journal_title：Applied and environmental microbiology

authors： Lawley B,Otal A,Moloney-Geany K,Diana A,Houghton L,Heath AM,Taylor RW,Tannock GW

更新日期：2019-09-17 00:00:00

abstract：:There is currently little information on nonphosphorylated sugar epimerases, which are of potential interest for producing rare sugars. We found a gene (the TM0416 gene) encoding a putative d-tagatose-3-epimerase-related protein from the hyperthermophilic bacterium Thermotoga maritima We overexpressed the TM0416 gene ...

journal_title：Applied and environmental microbiology

authors： Shin SM,Cao TP,Choi JM,Kim SB,Lee SJ,Lee SH,Lee DW

更新日期：2017-05-01 00:00:00

abstract：:Medicinal plants are valuable resources of natural antimicrobial materials. A novel small protein with antimicrobial activities, designated LJAMP1, was purified from the seeds of a medicinal herb, motherwort (Leonurus japonicus Houtt). LJAMP1 is a heat-stable protein with a molecular mass of 7.8 kDa and a determined i...

journal_title：Applied and environmental microbiology

authors： Yang X,Xiao Y,Wang X,Pei Y

更新日期：2007-02-01 00:00:00

abstract：:Produce isolates of the Escherichia coli Ont:H52 serotype carried Shiga toxin 1 and stable toxin genes but only expressed Stx1. These strains had pulsed-field gel electrophoresis profiles that were 90% homologous to clinical Ont:H52 strains that had identical phenotypes and genotypes. All Ont:H52 strains had identical...

journal_title：Applied and environmental microbiology

authors： Monday SR,Keys C,Hanson P,Shen Y,Whittam TS,Feng P

更新日期：2006-04-01 00:00:00

abstract：:A group-specific PCR-based denaturing gradient gel electrophoresis (DGGE) method was developed and combined with group-specific clone library analysis to investigate the diversity of the Clostridium leptum subgroup in human feces. PCR products (length, 239 bp) were amplified using C. leptum cluster-specific primers an...

journal_title：Applied and environmental microbiology

authors： Shen J,Zhang B,Wei G,Pang X,Wei H,Li M,Zhang Y,Jia W,Zhao L

更新日期：2006-08-01 00:00:00

abstract：:Heat-treated Escherichia coli producing Thermus polyphosphate kinase regenerated ATP by using exogenous polyphosphate. This recombinant could be used as a platform to produce valuable compounds in combination with thermostable phosphorylating or energy-requiring enzymes. In this work, we demonstrated the production of...

journal_title：Applied and environmental microbiology

authors： Iwamoto S,Motomura K,Shinoda Y,Urata M,Kato J,Takiguchi N,Ohtake H,Hirota R,Kuroda A

更新日期：2007-09-01 00:00:00

abstract：:The expression of xenobiotic-degradative genes in indigenous bacteria or in bacteria introduced into an ecosystem is essential for the successful bioremediation of contaminated environments. The maintenance of naphthalene utilization activity is studied in Pseudomonas putida (ATCC 17484) and an Alcaligenes sp. (strain...

journal_title：Applied and environmental microbiology

authors： Guerin WF,Boyd SA

更新日期：1995-11-01 00:00:00

abstract：:The effects of mineral fertilizer (NPK) and organic manure on the community structure of soil ammonia-oxidizing bacteria (AOB) was investigated in a long-term (16-year) fertilizer experiment. The experiment included seven treatments: organic manure, half organic manure N plus half fertilizer N, fertilizer NPK, fertili...

journal_title：Applied and environmental microbiology

authors： Chu H,Fujii T,Morimoto S,Lin X,Yagi K,Hu J,Zhang J

更新日期：2007-01-01 00:00:00

abstract：:Size changes during starvation of 17 marine bacterial isolates at a solid-water interface and in the liquid phase were examined. Twelve rod-shaped, hydrophilic bacteria decreased in size more rapidly at the solid surface than in the liquid phase, a result parallel to that observed previously for one of the strains at ...

journal_title：Applied and environmental microbiology

authors： Humphrey B,Kjelleberg S,Marshall KC

更新日期：1983-01-01 00:00:00

abstract：:Glycerol fermentation by Enterobacter agglomerans revealed that both growth and 1,3-propanediol production ceased after consumption of about 430 mM glycerol, irrespective of the initial glycerol content. This phenomenon was assigned to the production of 3-hydroxypropionaldehyde, which was identified by proton nuclear ...

journal_title：Applied and environmental microbiology

authors： Barbirato F,Grivet JP,Soucaille P,Bories A

更新日期：1996-04-01 00:00:00

abstract：:Juvenile oyster disease (JOD) causes significant annual mortalities of hatchery-produced Eastern oysters, Crassostrea virginica, cultured in the Northeast. We have reported that a novel species of the alpha-proteobacteria Roseobacter group (designated CVSP) was numerically dominant in JOD-affected animals sampled duri...

journal_title：Applied and environmental microbiology

authors： Boettcher KJ,Barber BJ,Singer JT

更新日期：2000-09-01 00:00:00