Purification, properties, and sequence specificity of SslI, a new type II restriction endonuclease from Streptococcus salivarius subsp. thermophilus.

Abstract:

:SslI, a type II restriction endonuclease, was purified from Streptococcus salivarius subsp. thermophilus strain BSN 45. SslI is an isoschizomer of BstNI. SslI activity was maximum at pH 8.8, 0 to 50 mM NaCl, 2 to 8 mM Mg2+, and 42 degrees C. Activity against phage DNA in vitro was demonstrated.

journal_name

Appl Environ Microbiol

authors

Benbadis L,Garel JR,Hartley DL

doi

10.1128/AEM.57.12.3677-3678.1991

subject

Has Abstract

pub_date

1991-12-01 00:00:00

pages

3677-8

issue

12

eissn

0099-2240

issn

1098-5336

journal_volume

57

pub_type

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