Development of a thermally regulated broad-spectrum promoter system for use in pathogenic gram-positive species.

abstract：:To understand the continuous problems that Escherichia coli O157:H7 causes as food pathogen, this study assessed global gene regulation in bacteria growing on meat. Since FlhD/FlhC of E. coli K-12 laboratory strains was previously established as a major control point in transducing signals from the environment to seve...

journal_title：Applied and environmental microbiology

authors： Sule P,Horne SM,Logue CM,Prüss BM

更新日期：2011-06-01 00:00:00

abstract：:The occurrence and distribution of magnetotactic bacteria (MB) were studied as a function of the physical and chemical conditions in meromictic Salt Pond, Falmouth, Mass., throughout summer 2002. Three dominant MB morphotypes were observed to occur within the chemocline. Small microaerophilic magnetite-producing cocci...

journal_title：Applied and environmental microbiology

authors： Simmons SL,Sievert SM,Frankel RB,Bazylinski DA,Edwards KJ

更新日期：2004-10-01 00:00:00

abstract：:Although tractable model organisms are essential to characterize the molecular mechanisms of evolution and adaptation, the ecological relevance of their behavior is not always clear because certain traits are easily lost during long-term laboratory culturing. Here, we demonstrate that despite their long tenure in the ...

journal_title：Applied and environmental microbiology

authors： Beer KD,Wurtmann EJ,Pinel N,Baliga NS

更新日期：2014-03-01 00:00:00

abstract：:A toluene-degrading sulfate-reducing bacterium, strain Tol2, was isolated from marine sediment under strictly anoxic conditions. Toluene was toxic if applied directly to the medium at concentrations higher than 0.5 mM. To provide toluene continuously at a nontoxic concentration, it was supplied in an inert hydrophobic...

journal_title：Applied and environmental microbiology

authors： Rabus R,Nordhaus R,Ludwig W,Widdel F

更新日期：1993-05-01 00:00:00

abstract：:The model organism for polyhydroxybutyrate (PHB) biosynthesis, Ralstonia eutropha H16, possesses multiple isoenzymes of granules coating phasins as well as of PHB depolymerases, which degrade accumulated PHB under conditions of carbon limitation. In this study, recombinant Escherichia coli BL21(DE3) strains were used ...

journal_title：Applied and environmental microbiology

authors： Eggers J,Steinbüchel A

更新日期：2014-12-01 00:00:00

abstract：:Dental caries is one of the most common diseases worldwide. Bacteria and fungi are both commensals in the oral cavity; however, most research regarding caries has focused on bacterial impacts. The oral fungal mycobiome associated with caries is not well characterized, and its role in disease is unclear. ITS1 amplicon ...

journal_title：Applied and environmental microbiology

authors： O'Connell LM,Santos R,Springer G,Burne RA,Nascimento MM,Richards VP

更新日期：2020-03-18 00:00:00

abstract：:Vibrio vulnificus is a human pathogen associated with consumption of raw oysters. During the colder months the organism apparently enters a viable but nonculturable state and thus cannot be cultured by ordinary bacteriological methods. For this reason, another means of detecting this bacterium is necessary. In the pre...

journal_title：Applied and environmental microbiology

authors： Brauns LA,Hudson MC,Oliver JD

更新日期：1991-09-01 00:00:00

abstract：:When a combination of hydrogen peroxide and hypochlorite was used to surface sterilize rice seeds, a 10(2)- to 10(4)-fold decrease in CFU was observed during the first 15 h after inoculation of the rice rhizosphere organism Burkholderia vietnamiensis TVV75. This artifact could not be eliminated simply by rinsing the s...

journal_title：Applied and environmental microbiology

authors： Miché L,Balandreau J

更新日期：2001-07-01 00:00:00

abstract：:We reported recently a unique fungal structure, called the spiny ball, on the vegetative hyphae of Coprinus comatus (O. F. Müll.:Fr.) Pers. Although some observations regarding the role of this structure were presented, its function remained largely unknown. In this study, we showed that purified (isolated and washed)...

journal_title：Applied and environmental microbiology

authors： Luo H,Liu Y,Fang L,Li X,Tang N,Zhang K

更新日期：2007-06-01 00:00:00

abstract：:The aim of this work was to develop a strategy to isolate a morphologically stable mutant of Streptomyces ambofaciens ATCC 15154 which produced high titers of spiramycin. The rationale was to grow a nitrosoguanidine-mutated population for many generations under nonselective conditions followed by two cycles of protopl...

journal_title：Applied and environmental microbiology

authors： Ford LM,Eaton TE,Godfrey OW

更新日期：1990-11-01 00:00:00

abstract：:Treatment of secondary effluents with hydrogen peroxide (10 mg/liter)-ascorbic acid (10 mg/liter)-Cu2+ (0.5 mg/liter) for 60 min resulted in around 99% reduction of the initial plate count. Hydrogen peroxide could be replaced by other peroxygen compounds; ascorbic acid could be replaced by other reducing agents, of wh...

journal_title：Applied and environmental microbiology

authors： Ragab-Depre NJ

更新日期：1982-09-01 00:00:00

abstract：:Microbial reductive dechlorination of [1,2-14C]trichloroethene to [14C]cis-dichloroethene and [14C]vinyl chloride was observed at 4 degrees C in anoxic microcosms prepared with cold temperature-adapted aquifer and river sediments from Alaska. Microbial anaerobic oxidation of [1,2-14C]cis-dichloroethene and [1,2-14C]vi...

journal_title：Applied and environmental microbiology

authors： Bradley PM,Richmond S,Chapelle FH

更新日期：2005-10-01 00:00:00

abstract：:Streptomyces exfoliatus SMF19 produced two types of extracellular beta-lactamase-inhibiting proteins, 48 (beta LIP-I) and 33 (beta LIP-II) kDa. The inhibition mode of beta LIP-I on Bacto Penase and benzylpenicillin as substrates was uncompetitive (i.e., both Km and Vmax were changed), while that of beta LIP-II was non...

journal_title：Applied and environmental microbiology

authors： Kim MK,Lee KJ

更新日期：1994-03-01 00:00:00

abstract：:Thermoanaerobacter sp. strain X514 has great potential in biotechnology due to its capacity to ferment a range of C(5) and C(6) sugars to ethanol and other metabolites under thermophilic conditions. This study investigated the central metabolism of strain X514 via (13)C-labeled tracer experiments using either glucose ...

journal_title：Applied and environmental microbiology

authors： Feng X,Mouttaki H,Lin L,Huang R,Wu B,Hemme CL,He Z,Zhang B,Hicks LM,Xu J,Zhou J,Tang YJ

更新日期：2009-08-01 00:00:00

abstract：:Bacillus cereus endospores germinate in response to particular nutrients. Spores are able to sense these nutrients in the environment by receptors encoded by the gerA family of operons. Analysis of the Bacillus cereus ATCC 14579 genome revealed seven gerA family homologues. Using a transposon Tn917-based insertional m...

journal_title：Applied and environmental microbiology

authors： Hornstra LM,de Vries YP,de Vos WM,Abee T,Wells-Bennik MH

更新日期：2005-02-01 00:00:00

abstract：:Wood-degrading fungi use a sequence of oxidative and hydrolytic mechanisms to loosen lignocellulose and then release and metabolize embedded sugars. These temporal sequences have recently been mapped at high resolution using directional growth on wood wafers, revealing previously obscured dynamics as fungi progressive...

journal_title：Applied and environmental microbiology

authors： Presley GN,Panisko E,Purvine SO,Schilling JS

更新日期：2018-08-01 00:00:00

abstract：:In order to elucidate interactions between sulfate reduction and dechlorination, we systematically evaluated the effects of different concentrations of sulfate and sulfide on reductive dechlorination by isolates, constructed consortia, and enrichments containing Dehalococcoides sp. Sulfate (up to 5 mM) did not inhibit...

journal_title：Applied and environmental microbiology

authors： Mao X,Polasko A,Alvarez-Cohen L

更新日期：2017-03-31 00:00:00

abstract：:Broad-substrate-range monooygenase enzymes, including toluene-4-monooxygenase (T4MO), can catalyze the oxidation of indole. The indole oxidation products can then condense to form the industrially important dye indigo. Site-directed mutagenesis of T4MO resulted in the creation of T4MO isoforms with altered pigment pro...

journal_title：Applied and environmental microbiology

authors： McClay K,Boss C,Keresztes I,Steffan RJ

更新日期：2005-09-01 00:00:00

abstract：:Across evolution, N-glycosylation involves oligosaccharyltransferases that transfer lipid-linked glycans to selected Asn residues of target proteins. While these enzymes catalyze similar reactions in each domain, differences exist in terms of the chemical composition, length and degree of phosphorylation of the lipid ...

journal_title：Applied and environmental microbiology

authors： Cohen-Rosenzweig C,Guan Z,Shaanan B,Eichler J

更新日期：2014-01-01 00:00:00

abstract：:Tannins are a diverse group of plant-produced, polyphenolic compounds with metal-chelating and antimicrobial properties that are prevalent in many soils. Using transcriptomics, we determined that tannic acid, a form of hydrolysable tannin, broadly affects the expression of genes involved in iron and zinc homeostases, ...

journal_title：Applied and environmental microbiology

authors： Lim CK,Penesyan A,Hassan KA,Loper JE,Paulsen IT

更新日期：2013-05-01 00:00:00

abstract：:Bacillus thuringiensis serovar israelensis (B. thuringiensis subsp. israelensis) produces four insecticidal crystal proteins (ICPs) (Cry4A, Cry4B, Cry11A, and Cyt1A). Toxicity of recombinant B. thuringiensis subsp. israelensis strains expressing only one of the toxins was determined with first instars of Tipula paludo...

journal_title：Applied and environmental microbiology

authors： Oestergaard J,Ehlers RU,Martínez-Ramírez AC,Real MD

更新日期：2007-06-01 00:00:00

abstract：:Lactococcin 972 (Lcn972) is a nonlantibiotic bacteriocin that inhibits septum biosynthesis in Lactococcus lactis rather than forming pores in the cytoplasmic membrane. In this study, a deeper analysis of the molecular basis of the mode of action of Lcn972 was performed. Of several lipid cell wall precursors, only lipi...

journal_title：Applied and environmental microbiology

authors： Martínez B,Böttiger T,Schneider T,Rodríguez A,Sahl HG,Wiedemann I

更新日期：2008-08-01 00:00:00

abstract：:While some species in the Bacillus cereus group are well-characterized human pathogens (e.g., B. anthracis and B. cereus sensu stricto), the pathogenicity of other species (e.g., B. pseudomycoides) either has not been characterized or is presently not well understood. To provide an updated characterization of the path...

journal_title：Applied and environmental microbiology

authors： Miller RA,Jian J,Beno SM,Wiedmann M,Kovac J

更新日期：2018-03-01 00:00:00

abstract：:The variable-region genes of monoclonal antibody against Bacillus cereus spores were cloned from mouse hybridoma cells by reverse transcription-PCR. The heavy- and light-chain variable-region genes were connected by a 45-base linker DNA to allow folding of the fusion protein into a functional tertiary structure. For d...

journal_title：Applied and environmental microbiology

authors： Koo K,Foegeding PM,Swaisgood HE

更新日期：1998-07-01 00:00:00

abstract：:A simple, efficient procedure for removing lactic acid and for reducing nonprotein nitrogen and ash in lactic acid whey has been developed. The procedure consists of culturing Candida ingens on the whey. This organism could assimilate >98% of the lactic acid and approximately 40% of the nonprotein nitrogen. Ash reduct...

journal_title：Applied and environmental microbiology

authors： Ruiz LP,Gurnsey JC,Short JL

更新日期：1978-04-01 00:00:00

abstract：:Since the food-borne pathogen Listeria monocytogenes is common in dairy farm environments, it is likely that phages infecting this bacterium ("listeriaphages") are abundant on dairy farms. To better understand the ecology and diversity of listeriaphages on dairy farms and to develop a diverse phage collection for furt...

journal_title：Applied and environmental microbiology

authors： Vongkamjan K,Switt AM,den Bakker HC,Fortes ED,Wiedmann M

更新日期：2012-12-01 00:00:00

abstract：:The reporter transposon-based system TnFuZ was used to identify exported proteins of the animal pathogen Corynebacterium pseudotuberculosis. Thirty-four out of 1,500 mutants had detectable alkaline phosphatase (PhoZ) activity. This activity was from 21 C. pseudotuberculosis loci that code for fimbrial and transport su...

journal_title：Applied and environmental microbiology

authors： Dorella FA,Estevam EM,Pacheco LG,Guimarães CT,Lana UG,Gomes EA,Barsante MM,Oliveira SC,Meyer R,Miyoshi A,Azevedo V

更新日期：2006-11-01 00:00:00

abstract：:Methanol is already an important carbon feedstock in the chemical industry, but it has found only limited application in biotechnological production processes. This can be mostly attributed to the inability of most microbial platform organisms to utilize methanol as a carbon and energy source. With the aim to turn met...

journal_title：Applied and environmental microbiology

authors： Witthoff S,Schmitz K,Niedenführ S,Nöh K,Noack S,Bott M,Marienhagen J

更新日期：2015-03-01 00:00:00

abstract：:We determined the effects of grain size and nutritional conditions on the penetration rate and metabolic activity of Escherichia coli strains in anaerobic, nutrient-saturated chambers packed with different sizes of glass beads (diameters, 116 to 767 mum) under static conditions. The chambers had nearly equal porositie...

journal_title：Applied and environmental microbiology

authors： Sharma PK,McInerney MJ

更新日期：1994-05-01 00:00:00

abstract：:In this study, we describe the association of three Campylobacter jejuni metabolism-related traits, γ-glutamyl-transpeptidase (GGT), fucose permease (fucP), and secreted L-asparaginase [ansB(s)], with multilocus sequence types (STs). A total of 710 C. jejuni isolates with known STs were selected and originated from hu...

journal_title：Applied and environmental microbiology

authors： de Haan CP,Llarena AK,Revez J,Hänninen ML

更新日期：2012-08-01 00:00:00