Mutations of toluene-4-monooxygenase that alter regiospecificity of indole oxidation and lead to production of novel indigoid pigments.

abstract：UNLABELLED:Clostridium perfringens type A is a common source of foodborne illness (FBI) in humans. Vegetative cells sporulate in the small intestinal tract and produce the major pathogenic factor C. perfringens enterotoxin. Although sporulation plays a critical role in the pathogenesis of FBI, the mechanisms inducing s...

journal_title：Applied and environmental microbiology

authors： Yasugi M,Okuzaki D,Kuwana R,Takamatsu H,Fujita M,Sarker MR,Miyake M

更新日期：2016-05-02 00:00:00

abstract：:In soil, Acidobacteria constitute on average 20% of all bacteria, are highly diverse, and are physiologically active in situ. However, their individual functions and interactions with higher taxa in soil are still unknown. Here, potential effects of land use, soil properties, plant diversity, and soil nanofauna on aci...

journal_title：Applied and environmental microbiology

authors： Naether A,Foesel BU,Naegele V,Wüst PK,Weinert J,Bonkowski M,Alt F,Oelmann Y,Polle A,Lohaus G,Gockel S,Hemp A,Kalko EK,Linsenmair KE,Pfeiffer S,Renner S,Schöning I,Weisser WW,Wells K,Fischer M,Overmann J,Friedric

更新日期：2012-10-01 00:00:00

abstract：:The Mediterranean fruit fly (Ceratitis capitata) is a cosmopolitan pest of hundreds of species of commercial and wild fruits. It is considered a major economic pest of commercial fruits in the world. Adult Mediterranean fruit flies feed on all sorts of protein sources, including animal excreta, in order to develop egg...

journal_title：Applied and environmental microbiology

authors： Sela S,Nestel D,Pinto R,Nemny-Lavy E,Bar-Joseph M

更新日期：2005-07-01 00:00:00

abstract：:The production of biofuels from lignocellulose yields a substantial lignin by-product stream that currently has few applications. Biological conversion of lignin-derived compounds into chemicals and fuels has the potential to improve the economics of lignocellulose-derived biofuels, but few microbes are able both to c...

journal_title：Applied and environmental microbiology

authors： Clarkson SM,Giannone RJ,Kridelbaugh DM,Elkins JG,Guss AM,Michener JK

更新日期：2017-08-31 00:00:00

abstract：:A spontaneous rpsL mutant of Thermus thermophilus was isolated in a search for new selection markers for this organism. This new allele, named rpsL1, encodes a K47R/K57E double mutant S12 ribosomal protein that confers a streptomycin-dependent (SD) phenotype to T. thermophilus. Models built on the available three-dime...

journal_title：Applied and environmental microbiology

authors： Blas-Galindo E,Cava F,López-Viñas E,Mendieta J,Berenguer J

更新日期：2007-08-01 00:00:00

abstract：:Dual-trap laser tweezers Raman spectroscopy (LTRS) and elastic light scattering (ELS) were used to investigate dynamic processes during high-temperature treatment of individual spores of Bacillus cereus, Bacillus megaterium, and Bacillus subtilis in water. Major conclusions from these studies included the following. (...

journal_title：Applied and environmental microbiology

authors： Zhang P,Kong L,Setlow P,Li YQ

更新日期：2010-03-01 00:00:00

abstract：:Four different green fluorescent protein (GFP)-based whole-cell biosensors were created based on the DNA damage inducible SOS response of Escherichia coli in order to evaluate the sensitivity of individual SOS promoters toward genotoxic substances. Treatment with the known carcinogen N-methyl-N'-nitro-N-nitrosoguanidi...

journal_title：Applied and environmental microbiology

authors： Norman A,Hestbjerg Hansen L,Sørensen SJ

更新日期：2005-05-01 00:00:00

abstract：:A Coxiella-type microbe occurs at 100% frequency in all Amblyomma americanum ticks thus far tested. Using laboratory-reared ticks free of other microbes, we identified the Amblyomma-associated Coxiella microbe in several types of tissue and at various stages of the life cycle of A. americanum by 16S rRNA gene sequenci...

journal_title：Applied and environmental microbiology

authors： Klyachko O,Stein BD,Grindle N,Clay K,Fuqua C

更新日期：2007-10-01 00:00:00

abstract：:Escherichia coli, a commensal bacterium from the intestinal tracts of humans and vertebrate animals, has been used as one of two bacterial indicators of fecal contamination, along with intestinal enterococci, to monitor the microbiological quality of water. However, water environments are now recognized as a secondary...

journal_title：Applied and environmental microbiology

authors： Berthe T,Ratajczak M,Clermont O,Denamur E,Petit F

更新日期：2013-08-01 00:00:00

abstract：:Although only partially understood, multicellular behavior is relatively common in bacterial pathogens. Bacterial aggregates can resist various host defenses and colonize their environment more efficiently than planktonic cells. For the waterborne pathogen Legionella pneumophila, little is known about the roles of aut...

journal_title：Applied and environmental microbiology

authors： Abdel-Nour M,Duncan C,Prashar A,Rao C,Ginevra C,Jarraud S,Low DE,Ensminger AW,Terebiznik MR,Guyard C

更新日期：2014-02-01 00:00:00

abstract：:Phylogeny and the distribution of symbiotic bacteria in the mixed segment of the wood-eating termite Nasutitermes takasagoensis (Shiraki) were studied. Bacterial 16S rRNA genes (rDNA) were amplified from the mixed segment of the gut by PCR, and two kinds of sequences were identified. The phylogenetic tree was construc...

journal_title：Applied and environmental microbiology

authors： Tokuda G,Yamaoka I,Noda H

更新日期：2000-05-01 00:00:00

abstract：:Botulinum neurotoxin (BoNT) serotype B (BoNT/B) is one of the serotypes of BoNT that causes deadly human botulism, though it is used clinically for treatment of many neuromuscular diseases. BoNT/B is produced by Clostridium botulinum, and it is secreted along with a group of neurotoxin-associated proteins (NAPs) in th...

journal_title：Applied and environmental microbiology

authors： Wang HH,Riding S,Lindo P,Singh BR

更新日期：2010-10-01 00:00:00

abstract：:The effects in the chicken embryo assay of four Alternaria metabolites (alternariol [AOH], alternariol methyl ether [AME], altenuene [ALT], and tenuazonic acid [TA]) were investigated. Administered to 7-day-old chicken embryos by yolk sac injection, AOH, AME, and ALT caused no mortality or teratogenic effect at doses ...

journal_title：Applied and environmental microbiology

authors： Griffin GF,Chu FS

更新日期：1983-12-01 00:00:00

abstract：:A method capable of detecting as little as 0.11 U of xylanase activity in polyacrylamide gels was developed. The method entails incubation of protein gels in contact with substrate gels containing unmodified xylan, followed by immersion of substrate gels in 95% ethanol. Resulting zymograms contain transparent bands co...

journal_title：Applied and environmental microbiology

authors： Royer JC,Nakas JP

更新日期：1990-06-01 00:00:00

abstract：:The structure and composition of the oocyst wall are primary factors determining the survival and hydrologic transport of Cryptosporidium parvum oocysts outside the host. Microscopic and biochemical analyses of whole oocysts and purified oocyst walls were undertaken to better understand the inactivation kinetics and h...

journal_title：Applied and environmental microbiology

authors： Jenkins MB,Eaglesham BS,Anthony LC,Kachlany SC,Bowman DD,Ghiorse WC

更新日期：2010-03-01 00:00:00

abstract：:A mixed culture of nitrate-reducing bacteria degraded o-cresol in the presence of toulene as a primary growth substrate. No degradation of o-cresol was observed in the absence of toluene or when the culture grew on p-cresol and 2,4-dimethylphenol. In batch cultures, the degradation of o-cresol started after toluene wa...

journal_title：Applied and environmental microbiology

authors： Flyvbjerg J,Jørgensen C,Arvin E,Jensen BK,Olsen SK

更新日期：1993-07-01 00:00:00

abstract：:The presence of enteroviruses in oysters and oyster-harvesting waters of the Texas Gulf coast was monitored over a period of 10 months. Viruses were detected in water and oyster samples obtained from areas both open and closed to shellfish harvesting. Viruses were detected periodically in waters that met current bacte...

journal_title：Applied and environmental microbiology

authors： Goyal SM,Gerba CP,Melnick JL

更新日期：1979-03-01 00:00:00

abstract：:The ability of yeasts to ferment cellodextrins is rare. Candida wickerhamii is able to use these sugars for alcohol production because of a cell-bound, extracellular, beta-glucosidase that is unusual by not being inhibited by glucose. A cDNA expression library in lambda phage was prepared with mRNA isolated from cello...

journal_title：Applied and environmental microbiology

authors： Skory CD,Freer SN

更新日期：1995-02-01 00:00:00

abstract：:A polyphasic approach involving cultivation, direct viable counts, rRNA-based phylogenetic classification, and in situ probing was applied for the characterization of the dominant microbial population in a municipal drinking water distribution system. A total of 234 bacterial strains cultivated on R2A medium were scre...

journal_title：Applied and environmental microbiology

authors： Kalmbach S,Manz W,Szewzyk U

更新日期：1997-11-01 00:00:00

abstract：:Rhodococcus equi is a leading cause of severe pneumonia in foals. Standard treatment is dual antimicrobial therapy with a macrolide and rifampin, but the emergence of macrolide- and rifampin-resistant R. equi isolates is an increasing problem. The objective of this study was to determine the effect of macrolide and/or...

journal_title：Applied and environmental microbiology

authors： Willingham-Lane JM,Berghaus LJ,Berghaus RD,Hart KA,Giguère S

更新日期：2019-03-22 00:00:00

abstract：:Cryptosporidium parvum is an apicomplexan parasite that infects humans and ruminants. C. parvum isolated from cattle in northeastern Turkey and in Israel was genotyped using multiple polymorphic genetic markers, and the two populations were compared to assess the effect of cattle husbandry on the parasite's population...

journal_title：Applied and environmental microbiology

authors： Tanriverdi S,Markovics A,Arslan MO,Itik A,Shkap V,Widmer G

更新日期：2006-04-01 00:00:00

abstract：:During the summer of 1981, 3,887 sucrose-negative vibrios were isolated from seawater, sediment, plankton, and animal samples taken from 80 sites from Miami, Fla., to Portland, Maine. Of these, 4.2% were able to ferment lactose. The lactose-positive strains isolated from the various samples correlated positively with ...

journal_title：Applied and environmental microbiology

authors： Oliver JD,Warner RA,Cleland DR

更新日期：1983-03-01 00:00:00

abstract：:Agmatine, a significant polyamine in bacteria and plants, mostly arises from the decarboxylation of arginine. The functional importance of agmatine in fungi is poorly understood. The metabolism of agmatine and related guanidinium group-containing compounds in Aspergillus niger was explored through growth, metabolite, ...

journal_title：Applied and environmental microbiology

authors： Kumar S,Saragadam T,Punekar NS

更新日期：2015-08-15 00:00:00

abstract：:The facultative anaerobe Thauera aromatica strain AR-1 uses 3,5-dihydroxybenzoate (3,5-DHB) as a sole carbon and energy source under anoxic conditions using an unusual oxidative strategy to overcome aromatic ring stability. A 25-kb gene cluster organized in four main operons encodes the anaerobic degradation pathway f...

journal_title：Applied and environmental microbiology

authors： Pacheco-Sánchez D,Molina-Fuentes Á,Marín P,Díaz-Romero A,Marqués S

更新日期：2019-01-09 00:00:00

abstract：:Enterocytozoon bieneusi is an emerging and clinically significant enteric parasite infecting humans and animals and can cause life-threatening diarrhea in immunocompromised people. Pigs are considered to be one of the main reservoir hosts of E. bieneusi based on their high prevalence rates and zoonotic genotypes in pi...

journal_title：Applied and environmental microbiology

authors： Zhao W,Zhang W,Yang F,Cao J,Liu H,Yang D,Shen Y,Liu A

更新日期：2014-06-01 00:00:00

abstract：:A betaproteobacterium within the family Rhodocyclaceae previously identified as a pyrene degrader via stable-isotope probing (SIP) of contaminated soil (designated pyrene group 1 or PG1) was cultivated as the dominant member of a mixed bacterial culture. A metagenomic library was constructed, and the largest contigs w...

journal_title：Applied and environmental microbiology

authors： Singleton DR,Hu J,Aitken MD

更新日期：2012-05-01 00:00:00

abstract：:Lantibiotics, such as nisin and subtilin, are lanthionine-containing peptides that exhibit antimicrobial as well as pheromone-like autoinducing activity. Autoinduction is specific for each lantibiotic, and reporter systems for nisin and subtilin autoinduction are available. In this report, we used the previously repor...

journal_title：Applied and environmental microbiology

authors： Fuchs SW,Jaskolla TW,Bochmann S,Kötter P,Wichelhaus T,Karas M,Stein T,Entian KD

更新日期：2011-03-01 00:00:00

abstract：:Isolated rumen bacteria were examined for growth and, where appropriate, for their ability to degrade cellulose in the presence of the hydroxycinnamic acids trans-p-coumaric acid and trans-ferulic acid and the hydroxybenzoic acids vanillic acid and 4-hydroxybenzoic acid. Ferulic and p-coumaric acids proved to be the m...

journal_title：Applied and environmental microbiology

authors： Chesson A,Stewart CS,Wallace RJ

更新日期：1982-09-01 00:00:00

abstract：:The aim of this work was to identify genes in Lactococcus lactis subsp. lactis IL1403 and Lactococcus lactis subsp. cremoris Wg2 important for adsorption of the 936-species phages bIL170 and phi 645, respectively. Random insertional mutagenesis of the two L. lactis strains was carried out with the vector pGh9:ISS1, an...

journal_title：Applied and environmental microbiology

authors： Dupont K,Janzen T,Vogensen FK,Josephsen J,Stuer-Lauridsen B

更新日期：2004-10-01 00:00:00

abstract：:Azotobacter vinelandii selectively utilizes three types of nitrogenase (molybdenum, vanadium, and iron only) to fix N2, with their expression regulated by the presence or absence of different metal cofactors in its environment. Each alternative nitrogenase isoenzyme is predicted to have different electron flux require...

journal_title：Applied and environmental microbiology

authors： Natzke J,Noar J,Bruno-Bárcena JM

更新日期：2018-08-01 00:00:00