Cloning and characterization of a gene encoding a cell-bound, extracellular beta-glucosidase in the yeast Candida wickerhamii.

abstract：:The gene cluster that codes for feedback-resistant aspartate kinase (lysCalpha and lysCbeta) and aspartate semialdehyde dehydrogenase (asd) was cloned from a mutant strain of Corynebacterium glutamicum. Its functional analysis by subcloning, enzyme assays, and type of aspartate kinase regulation enabled the isolation ...

journal_title：Applied and environmental microbiology

authors： Cremer J,Eggeling L,Sahm H

更新日期：1991-06-01 00:00:00

abstract：:Isolates of the obligately biotrophic fungus Uncinula necator cluster in three distinct genetic groups (groups I, II, and III). We designed PCR primers specific for these groups in order to monitor field populations of U. necator. We used the nucleotide sequences of the gene that encodes eburicol 14alpha-demethylase (...

journal_title：Applied and environmental microbiology

authors： Délye C,Ronchi V,Laigret F,Corio-Costet MF

更新日期：1999-09-01 00:00:00

abstract：:The bacterial rhizosphere communities of three host plants of the pathogenic fungus Verticillium dahliae, field-grown strawberry (Fragaria ananassa Duch.), oilseed rape (Brassica napus L.), and potato (Solanum tuberosum L.), were analyzed. We aimed to determine the degree to which the rhizosphere effect is plant depen...

journal_title：Applied and environmental microbiology

authors： Smalla K,Wieland G,Buchner A,Zock A,Parzy J,Kaiser S,Roskot N,Heuer H,Berg G

更新日期：2001-10-01 00:00:00

abstract：:The prokaryote community activity and structural characteristics within marine sediment sampled across a continental shelf area located off eastern Antarctica (66 degrees S, 143 degrees E; depth range, 709 to 964 m) were studied. Correlations were found between microbial biomass and aminopeptidase and chitinase rates,...

journal_title：Applied and environmental microbiology

authors： Bowman JP,McCammon SA,Gibson JA,Robertson L,Nichols PD

更新日期：2003-05-01 00:00:00

abstract：:Difluoromethane (HFC-32; DFM) is compared to acetylene and methyl fluoride as an inhibitor of methanotrophy in cultures and soils. DFM was found to be a reversible inhibitor of CH4 oxidation by Methylococcus capsulatus (Bath). Consumption of CH4 in soil was blocked by additions of low levels of DFM (0.03 kPa), and thi...

journal_title：Applied and environmental microbiology

authors： Miller LG,Sasson C,Oremland RS

更新日期：1998-11-01 00:00:00

abstract：:The presence of human-pathogenic, enteric bacteria on the surface and in the interior of raw produce is a significant health concern. Several aspects of the biology of the interaction between these bacteria and alfalfa (Medicago sativa) seedlings are addressed here. A collection of enteric bacteria associated with alf...

journal_title：Applied and environmental microbiology

authors： Dong Y,Iniguez AL,Ahmer BM,Triplett EW

更新日期：2003-03-01 00:00:00

abstract：:Bacteria, yeasts, and molds isolated from partially processed iceberg lettuce were taxonomically classified. The majority of bacterial isolates were gram-negative rods. Pseudomonas, Erwinia, and Serratia species were commonly found. Yeasts most frequently isolated from lettuce included members of the genera Candida, C...

journal_title：Applied and environmental microbiology

authors： Magnuson JA,King AD Jr,Török T

更新日期：1990-12-01 00:00:00

abstract：:The only major structural protein (35 kDa) of the lactococcal small isometric-headed bacteriophage ul36, a member of the P335 species, was isolated from a preparative sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis. Monoclonal antibodies (MAbs) were raised against the denatured 35-kDa protein. Six MAbs...

journal_title：Applied and environmental microbiology

authors： Moineau S,Bernier D,Jobin M,Hébert J,Klaenhammer TR,Pandian S

更新日期：1993-07-01 00:00:00

abstract：:The bacteriophage T4 denV gene encodes a well-characterized DNA repair enzyme involved in pyrimidine photodimer excision. We have discovered the first homologs of the denV gene in chlorella viruses, which are common in fresh water. This gene functions in vivo and also when cloned in Escherichia coli. Photodamaged viru...

journal_title：Applied and environmental microbiology

authors： Furuta M,Schrader JO,Schrader HS,Kokjohn TA,Nyaga S,McCullough AK,Lloyd RS,Burbank DE,Landstein D,Lane L,Van Etten JL

更新日期：1997-04-01 00:00:00

abstract：:Swimming in ocean water, including ocean water at beaches not impacted by known point sources of pollution, is an increasing health concern. This study was an initial evaluation of the presence of indicator microbes and pathogens and the association among the indicator microbes, pathogens, and environmental conditions...

journal_title：Applied and environmental microbiology

authors： Abdelzaher AM,Wright ME,Ortega C,Solo-Gabriele HM,Miller G,Elmir S,Newman X,Shih P,Bonilla JA,Bonilla TD,Palmer CJ,Scott T,Lukasik J,Harwood VJ,McQuaig S,Sinigalliano C,Gidley M,Plano LR,Zhu X,Wang JD,Fleming LE

更新日期：2010-02-01 00:00:00

abstract：:Several members of the glycoside hydrolase 61 (GH61) family of proteins have recently been shown to dramatically increase the breakdown of lignocellulosic biomass by microbial hydrolytic cellulases. However, purified GH61 proteins have neither demonstrable direct hydrolase activity on various polysaccharide or lignaci...

journal_title：Applied and environmental microbiology

authors： Langston JA,Shaghasi T,Abbate E,Xu F,Vlasenko E,Sweeney MD

更新日期：2011-10-01 00:00:00

abstract：:The effects of plasterboard composition on the growth and sporulation of Stachybotrys chartarum as well as on the inflammatory potential of the spores were studied. S. chartarum was grown on 13 modified plasterboards under saturated humidity conditions. The biomass was estimated by measuring the ergosterol content of ...

journal_title：Applied and environmental microbiology

authors： Murtoniemi T,Nevalainen A,Hirvonen MR

更新日期：2003-07-01 00:00:00

abstract：:The introduction of "solids" (i.e., complementary foods) to the milk-only diet in early infancy affects the development of the gut microbiota. The aim of this study was to determine whether a "baby-led" approach to complementary feeding that encourages the early introduction of an adult-type diet results in alteration...

journal_title：Applied and environmental microbiology

authors： Leong C,Haszard JJ,Lawley B,Otal A,Taylor RW,Szymlek-Gay EA,Fleming EA,Daniels L,Fangupo LJ,Tannock GW,Heath AM

更新日期：2018-08-31 00:00:00

abstract：:Adsorbed bovine serum albumin was resistant to exchange with beta-lactoglobulin, and when albumin was adsorbed from a mixture, its surface concentration increased with time. The passivating character of adsorbed albumin and its resistance to desorption were consistent with the level of Listeria monocytogenes adhesion ...

journal_title：Applied and environmental microbiology

authors： al-Makhlafi H,Nasir A,McGuire J,Daeschel M

更新日期：1995-05-01 00:00:00

abstract：:We report here the cloning and sequencing of the gene for proline dehydrogenase (putA) of Bradyrhizobium japonicum. An open reading frame coding for 1,016 amino acids was identified. The B. japonicum gene codes for a bifunctional protein with proline dehydrogenase and pyrroline-5-carboxylate (P5C) dehydrogenase activi...

journal_title：Applied and environmental microbiology

authors： Straub PF,Reynolds PH,Althomsons S,Mett V,Zhu Y,Shearer G,Kohl DH

更新日期：1996-01-01 00:00:00

abstract：:The present work describes the development of a superior strain of Corynebacterium glutamicum for diaminopentane (cadaverine) production aimed at the identification and deletion of the underlying unknown N-acetyldiaminopentane pathway. This acetylated product variant, recently discovered, is a highly undesired by-prod...

journal_title：Applied and environmental microbiology

authors： Kind S,Jeong WK,Schröder H,Zelder O,Wittmann C

更新日期：2010-08-01 00:00:00

abstract：:Evaluating different swabbing materials for spore recovery efficiency (RE) from steel surfaces, we recorded the maximum RE (71%) of 10(7) Bacillus subtilis spores with Tulips cotton buds, followed by Johnson's cotton buds and standard Hi-Media cotton, polyester, nylon, and foam (23%) swabs. Among cotton swabs, instant...

journal_title：Applied and environmental microbiology

authors： Thomas P,Mujawar MM,Upreti R,Sekhar AC

更新日期：2013-01-01 00:00:00

abstract：:The bphC and bphD genes of Pseudomonas putida involved in the catabolism of polychlorinated biphenyls or biphenyl were identified, localized, and studied for expression in Escherichia coli. This was achieved by cloning a 2.4-kilobase (kb) DNA fragment of recombinant cosmid pOH101 into HindIII site of pUC plasmids down...

journal_title：Applied and environmental microbiology

authors： Khan AA,Walia SK

更新日期：1990-04-01 00:00:00

abstract：:Salinity effects on microbial community structure and on potential rates of arsenate reduction, arsenite oxidation, sulfate reduction, denitrification, and methanogenesis were examined in sediment slurries from two California soda lakes. We conducted experiments with Mono Lake and Searles Lake sediments over a wide ra...

journal_title：Applied and environmental microbiology

authors： Kulp TR,Han S,Saltikov CW,Lanoil BD,Zargar K,Oremland RS

更新日期：2007-08-01 00:00:00

abstract：:The colonization of apple blossoms and leaves by Pseudomonas fluorescens EPS62e was monitored in greenhouse and field trials using cultivable cell counting and real-time PCR. The real-time PCR provided a specific quantitative method for the detection of strain EPS62e. The detection level was around 10(2) cells g (fres...

journal_title：Applied and environmental microbiology

authors： Pujol M,Badosa E,Manceau C,Montesinos E

更新日期：2006-04-01 00:00:00

abstract：:Microbial transformation of lapachol, a naturally occurring naphthoquinone, was carried out by Curvularia lunata (NRRL 2178). The fungus brings about oxidative cyclization of the substrate to dehydro-alpha-lapachone, which was isolated and characterized by nuclear magnetic resonance and mass spectral analyses; its str...

journal_title：Applied and environmental microbiology

authors： Otten S,Rosazza JP

更新日期：1979-08-01 00:00:00

abstract：:We have engineered Escherichia coli to overproduce saturated and monounsaturated aliphatic methyl ketones in the C₁₁ to C₁₅ (diesel) range; this group of methyl ketones includes 2-undecanone and 2-tridecanone, which are of importance to the flavor and fragrance industry and also have favorable cetane numbers (as we re...

journal_title：Applied and environmental microbiology

authors： Goh EB,Baidoo EE,Keasling JD,Beller HR

更新日期：2012-01-01 00:00:00

abstract：:We previously reported that overexpression of the soxS or robA gene causes in several Escherichia coli strains the acquisition of higher organic solvent tolerance and also increased resistance to a number of antibiotics (H. Nakajima, K. Kobayashi, M. Kobayashi, H. Asako, and R. Aono, Appl. Environ. Microbiol. 61:2302-...

journal_title：Applied and environmental microbiology

authors： Asako H,Nakajima H,Kobayashi K,Kobayashi M,Aono R

更新日期：1997-04-01 00:00:00

abstract：:We describe the development and validation of a method for the qualitative analysis of complex bifidobacterial communities based on PCR and denaturing gradient gel electrophoresis (DGGE). Bifidobacterium genus-specific primers were used to amplify an approximately 520-bp fragment from the 16S ribosomal DNA (rDNA), and...

journal_title：Applied and environmental microbiology

authors： Satokari RM,Vaughan EE,Akkermans AD,Saarela M,de Vos WM

更新日期：2001-02-01 00:00:00

abstract：:The intestinal contents of 59 Zairese freshwater fish were examined for the presence of potential human enteric pathogens. Edwardsiella tarda and Plesiomonas shigelloides were isolated from 57 and 59% of them, respectively. For both microorganisms there was a significant difference between the isolation rates from lak...

journal_title：Applied and environmental microbiology

authors： Van Damme LR,Vandepitte J

更新日期：1980-03-01 00:00:00

abstract：:We describe useful vectors to select double-crossover events directly in site-directed marker exchange mutagenesis in gram-negative bacteria. These vectors contain the gusA marker gene, providing colorimetric screens to identify bacteria harboring those sequences. The applicability of these vectors was shown by mappin...

journal_title：Applied and environmental microbiology

authors： Katzen F,Becker A,Ielmini MV,Oddo CG,Ielpi L

更新日期：1999-01-01 00:00:00

abstract：:Clostridium difficile causes a potentially fatal diarrheal disease through the production of its principal virulence factors, toxin A and toxin B. The tcdC gene is thought to encode a negative regulator of toxin production. Therefore, increased toxin production, and hence increased virulence, is often inferred in stra...

journal_title：Applied and environmental microbiology

authors： Cartman ST,Kelly ML,Heeg D,Heap JT,Minton NP

更新日期：2012-07-01 00:00:00

abstract：:Bioconversion of alpha-damascone (compound 1) was studied with four strains of Botrytis cinerea in grape must (pH 3.2). As biotransformation products of compound 1, 3-oxo-alpha-damascone, cis- and trans-3-hydroxy-alpha-damascone, gamma-damascenone, 3-oxo-8, 9-dihydro-alpha-damascone, and cis- and trans-3-hydroxy-8,9-d...

journal_title：Applied and environmental microbiology

authors： Schoch E,Benda I,Schreier P

更新日期：1991-01-01 00:00:00

abstract：:Pseudomonas fluorescens isolates 13525 and 2-79 were grown in Luria broth and low-nutrient medium (LNM). Pililike fibrils were very rarely produced in Luria broth but were abundantly produced in LNM. In LNM the pili were peritrichously distributed and had diameters ranging from 3 to 8 nm. Pili were purified from strai...

journal_title：Applied and environmental microbiology

authors： Vesper SJ

更新日期：1987-07-01 00:00:00

abstract：:Two different DNA fragments, one of 2.9 kilobases and the other of 5.1 kilobases, were cloned from Phytophthora citrophthora and showed no homology with DNA from plants and other related fungi. These DNA probes hybridized with DNA from 12 different P. citrophthora isolates obtained from a variety of hosts but did not ...

journal_title：Applied and environmental microbiology

authors： Goodwin PH,Kirkpatrick BC,Duniway JM

更新日期：1990-03-01 00:00:00