Production of Pili (Fimbriae) by Pseudomonas fluorescens and Correlation with Attachment to Corn Roots.

abstract：:Diversity among 130 strains of Bacillus polymyxa was studied; the bacteria were isolated by immunotrapping from nonrhizosphere soil (32 strains), rhizosphere soil (38 strains), and the rhizoplane (60 strains) of wheat plantlets growing in a growth chamber. The strains were characterized phenotypically by 63 auxanograp...

journal_title：Applied and environmental microbiology

authors： Mavingui P,Laguerre G,Berge O,Heulin T

更新日期：1992-06-01 00:00:00

abstract：:Genetic optimizations to achieve high-level production of three different proteins of medical importance for humans, granulocyte-macrophage colony-stimulating factor (GM-CSF), interferon alpha 2b (IFN-alpha2b), and single-chain antibody variable fragment (scFv-phOx), were investigated during high-cell-density cultivat...

journal_title：Applied and environmental microbiology

authors： Sletta H,Tøndervik A,Hakvåg S,Aune TE,Nedal A,Aune R,Evensen G,Valla S,Ellingsen TE,Brautaset T

更新日期：2007-02-01 00:00:00

abstract：:Profiles of dissolved O(2) and methane with increasing depth were generated for Lake Washington sediment, which suggested the zone of methane oxidation is limited to the top 0.8 cm of the sediment. Methane oxidation potentials were measured for 0.5-cm layers down to 1.5 cm and found to be relatively constant at 270 to...

journal_title：Applied and environmental microbiology

authors： Auman AJ,Stolyar S,Costello AM,Lidstrom ME

更新日期：2000-12-01 00:00:00

abstract：:Pseudomonas savastanoi pv. savastanoi strain NCPPB 3335 is a model bacterial pathogen for studying the molecular basis of disease production in woody hosts. We report the sequencing of the hrpS-to-hrpZ region of NCPPB 3335, which has allowed us to determine the phylogenetic position of this pathogen with respect to pr...

journal_title：Applied and environmental microbiology

authors： Pérez-Martínez I,Rodríguez-Moreno L,Lambertsen L,Matas IM,Murillo J,Tegli S,Jiménez AJ,Ramos C

更新日期：2010-06-01 00:00:00

abstract：:Marine microbiologists commonly assay lipase activities by using a synthetic fluorescent analog, 4-methylumbelliferyl (MUF)-oleate. The technique is convenient, but it is considered to be unspecific because of the structure of this analog. This study reports the design of a new specific and sensitive lipase assay base...

journal_title：Applied and environmental microbiology

authors： Bourguet N,Torréton JP,Galy O,Arondel V,Goutx M

更新日期：2003-12-01 00:00:00

abstract：:Bacillus thuringiensis produces large amounts of various pesticidal proteins during the stationary phase. In order to achieve a high yield and form crystals, some pesticidal proteins require the presence of other proteins. Helper protein P20 is required for efficient production of both the Cyt1A and Cry11A crystal pro...

journal_title：Applied and environmental microbiology

authors： Shao Z,Liu Z,Yu Z

更新日期：2001-12-01 00:00:00

abstract：:Members of a group of marine bacteria that is numerically important in coastal seawater and sediments were characterized with respect to their ability to transform organic and inorganic sulfur compounds. Fifteen strains representing the Roseobacter group (a phylogenetic cluster of marine bacteria in the alpha-subclass...

journal_title：Applied and environmental microbiology

authors： González JM,Kiene RP,Moran MA

更新日期：1999-09-01 00:00:00

abstract：:In this study, we report the cloning and expression of lipase gene from Pseudomonas fluorescens B52, a psychrotrophic spoilage bacterium isolated from refrigerated raw milk. Sequence analysis revealed one major open reading frame of 1,428 nucleotides that was predicted to encode a protein with a molecular weight of 50...

journal_title：Applied and environmental microbiology

authors： Tan Y,Miller KJ

更新日期：1992-04-01 00:00:00

abstract：:Bacterium T-52, cultured on ethylene glycol, readily oxidized glycolate and glyoxylate and exhibited elevated activities of ethylene glycol dehydrogenase and glycolate oxidase. Labeled glyoxylate was identified in reaction mixtures containing [C]-ethylene glycol, but no glycolate was detected. The most likely pathway ...

journal_title：Applied and environmental microbiology

authors： Caskey WH,Taber WA

更新日期：1981-07-01 00:00:00

abstract：:From Bradyrhizobium japonicum highly reiterated sequence-possessing (HRS) strains indigenous to Niigata and Tokachi in Japan with high copy numbers of the repeated sequences RSalpha and RSbeta (K. Minamisawa, T. Isawa, Y. Nakatsuka, and N. Ichikawa, Appl. Environ. Microbiol. 64:1845-1851, 1998), several insertion sequ...

journal_title：Applied and environmental microbiology

authors： Isawa T,Sameshima R,Mitsui H,Minamisawa K

更新日期：1999-08-01 00:00:00

abstract：:The biological impact of 24-h ("chronic") chromium(VI) [Cr(VI) or chromate] exposure on Shewanella oneidensis MR-1 was assessed by analyzing cellular morphology as well as genome-wide differential gene and protein expression profiles. Cells challenged aerobically with an initial chromate concentration of 0.3 mM in com...

journal_title：Applied and environmental microbiology

authors： Chourey K,Thompson MR,Morrell-Falvey J,Verberkmoes NC,Brown SD,Shah M,Zhou J,Doktycz M,Hettich RL,Thompson DK

更新日期：2006-09-01 00:00:00

abstract：:The temperate bacteriophage BK5-T was isolated from Streptococcus cremoris BK5 by induction with mitomycin C. Electron microscopy revealed that BK5-T DNA consists of linear molecules, ranging in size from 39.7 to 46 kilobase pairs. Restriction analysis of self-ligated BK5-T DNA showed that the ends of the DNA were not...

journal_title：Applied and environmental microbiology

authors： Lakshmidevi G,Davidson BE,Hillier AJ

更新日期：1988-04-01 00:00:00

abstract：:The bacterium Rhodopseudomonas palustris grows with the aromatic acid benzoate and the alicyclic acid cyclohexanecarboxylate (CHC) as sole carbon sources. The enzymatic steps in an oxygen-independent pathway for CHC degradation have been elucidated, but it was unknown how the CHC operon (badHI aliAB badK) encoding the...

journal_title：Applied and environmental microbiology

authors： Hirakawa H,Hirakawa Y,Greenberg EP,Harwood CS

更新日期：2015-07-01 00:00:00

abstract：:Five strains of mesophilic, facultatively organotrophic, ore-leaching eubacteria were isolated from solfatara fields in Iceland and a uranium mine in the Federal Republic of Germany. The new organisms are aerobic gram-negative rods. They can use sulfidic ores or elemental sulfur as sole energy source, indicating that ...

journal_title：Applied and environmental microbiology

authors： Huber H,Stetter KO

更新日期：1990-02-01 00:00:00

abstract：:During incubation of seawater in bottles, the decrease in dissolved oxygen is often nonlinear over time scales frequently used to measure respiration. Numbers of bacteria always increase, and rates of assimilation of dissolved leucine often increase exponentially. This suggests that sample handling disrupts the previo...

journal_title：Applied and environmental microbiology

authors： Pomeroy LR,Sheldon JE,Sheldon WM

更新日期：1994-01-01 00:00:00

abstract：:In many cities, the feral rock dove is an abundant bird species that can harbor Chlamydophila psittaci. We determined the prevalence and genotype of C. psittaci in fresh fecal samples from feral pigeons in Amsterdam, The Netherlands. The prevalence was 7.9% overall (26/331; 95% confidence interval, 5 to 11). Ten genot...

journal_title：Applied and environmental microbiology

authors： Heddema ER,Ter Sluis S,Buys JA,Vandenbroucke-Grauls CM,van Wijnen JH,Visser CE

更新日期：2006-06-01 00:00:00

abstract：:Total DNA from 21 collection or industrial Bifidobacterium strains was cleaved with various restriction endonucleases. Following electrophoresis, the fragments were subjected to Southern blot hybridization with a heterologous [alpha-32P]dCTP-labeled rDNA (genes coding for rRNA) 23S gene probe. The ribosomal patterns a...

journal_title：Applied and environmental microbiology

authors： Mangin I,Bourget N,Bouhnik Y,Bisetti N,Simonet JM,Decaris B

更新日期：1994-05-01 00:00:00

abstract：:Volume 61, no. 4, p. 1211, column 2, line 40: this sentence should read as follows. "The viable-culture methods for Legionella sp. detection, however, often fail, and the Centers for Disease Control and Prevention has turned, when necessary, to PCR for epidemiological investigations of Legionnaires' disease and Pontia...

journal_title：Applied and environmental microbiology

authors： Atlas RM,Williams JF,Huntington MK

更新日期：1996-04-01 00:00:00

abstract：:A new method for the extraction of bacterial DNA from soil has been developed. Soil samples of 50 g were dispersed, and bacteria were released by use of a cation-exchange resin; subsequently, bacteria were separated from soil particles by low-speed centrifugation and lysed with lysozyme and ionic detergent, and the DN...

journal_title：Applied and environmental microbiology

authors： Jacobsen CS,Rasmussen OF

更新日期：1992-08-01 00:00:00

abstract：:Stachybotrys atra is the etiologic agent of stachybotryotoxicosis, and this fungus and its trichothecene mycotoxins were recently implicated in an outbreak of unexplained illness in homes. S. atra was grown on sterile rice, autoclaved, dried, and then aerosolized by acoustic vibration. The distribution of particles (m...

journal_title：Applied and environmental microbiology

authors： Sorenson WG,Frazer DG,Jarvis BB,Simpson J,Robinson VA

更新日期：1987-06-01 00:00:00

abstract：:Many cyanobacterial strains are able to grow at a pH range from neutral to pH 10 or 11. Such alkaline conditions favor cyanobacterial growth (e.g., bloom formation), and cyanobacteria must have developed strategies to adjust to changes in CO2 concentration and ion availability. Synechocystis sp. strain PCC 6803 exhibi...

journal_title：Applied and environmental microbiology

authors： Summerfield TC,Sherman LA

更新日期：2008-09-01 00:00:00

abstract：:Marine infaunal burrows and tubes greatly enhance solute transport between sediments and the overlying water column and are sites of elevated microbial activity. Biotic and abiotic controls of the compositions and activities of burrow and tube microbial communities are poorly understood. The microbial communities in t...

journal_title：Applied and environmental microbiology

authors： Matsui GY,Ringelberg DB,Lovell CR

更新日期：2004-12-01 00:00:00

abstract：:The Atlantis Massif rises 4,000 m above the seafloor near the Mid-Atlantic Ridge and consists of rocks uplifted from Earth's lower crust and upper mantle. Exposure of the mantle rocks to seawater leads to their alteration into serpentinites. These aqueous geochemical reactions, collectively known as the process of ser...

journal_title：Applied and environmental microbiology

authors： Motamedi S,Orcutt BN,Früh-Green GL,Twing KI,Pendleton HL,Brazelton WJ

更新日期：2020-05-19 00:00:00

abstract：:A growth medium and test were developed for rapid detection of urease in fermentative anaerobic bacteria. Using nonselective rumen fluid roll-tube agar medium and the new test, it was confirmed that Peptostreptococcus productus is often the most numerous urease-forming species in human feces. Also, some fecal strains ...

journal_title：Applied and environmental microbiology

authors： Wozny MA,Bryant MP,Holdeman LV,Moore WE

更新日期：1977-05-01 00:00:00

abstract：:The growth of the filamentous fungus Aspergillus niger, a common food spoilage organism, is inhibited by the weak acid preservative sorbic acid (trans-trans-2,4-hexadienoic acid). Conidia inoculated at 10(5)/ml of medium showed a sorbic acid MIC of 4.5 mM at pH 4.0, whereas the MIC for the amount of mycelia at 24 h de...

journal_title：Applied and environmental microbiology

authors： Plumridge A,Hesse SJ,Watson AJ,Lowe KC,Stratford M,Archer DB

更新日期：2004-06-01 00:00:00

abstract：:The low-copy-number 8.7-kb plasmid pGIF3 of Streptomyces incarnatus was studied after cloning in the Escherichia coli vector pBR322; a restriction map was constructed. Southern blot analysis showed that pGIF3 in S. incarnatus occurs predominantly as integrated in a larger replicon. The plasmid carries a gene for thios...

journal_title：Applied and environmental microbiology

authors： Malina H,Robert-Gero M

更新日期：1992-03-01 00:00:00

abstract：:The arginine deiminase system in a variety of streptococci and in Pseudomonas aeruginosa was found to be unusually acid tolerant in that arginolysis occurred at pH values well below the minima for growth and glycolysis. The acid tolerance of the system allowed bacteria to survive potentially lethal acidification throu...

journal_title：Applied and environmental microbiology

authors： Marquis RE,Bender GR,Murray DR,Wong A

更新日期：1987-01-01 00:00:00

abstract：:Members of the genus Sphingomonas are important catalysts for removal of polycyclic aromatic hydrocarbons (PAHs) in soil, but their activity can be affected by various stress factors. This study examines the physiological and genome-wide transcription response of the phenanthrene-degrading Sphingomonas sp. strain LH12...

journal_title：Applied and environmental microbiology

authors： Fida TT,Breugelmans P,Lavigne R,Coronado E,Johnson DR,van der Meer JR,Mayer AP,Heipieper HJ,Hofkens J,Springael D

更新日期：2012-12-01 00:00:00

abstract：:We developed an improved method for cultivation-independent sorting of bacterial cells. The technique is based on labeling the target cells by in situ hybridization with polynucleotide transcript probes. Due to the probes' length, part of the probe remains outside the cell and can subsequently be used to capture the c...

journal_title：Applied and environmental microbiology

authors： Zwirglmaier K,Ludwig W,Schleifer KH

更新日期：2004-01-01 00:00:00

abstract：:Cyanobacterial hepatotoxins, microcystins, are specific inhibitors of serine/threonine protein phosphatases and potent tumor promoters. They have caused several poisonings of animals and also pose a health hazard for humans through the use of water for drinking and recreation. Different strains of the same cyanobacter...

journal_title：Applied and environmental microbiology

authors： Rapala J,Sivonen K,Lyra C,Niemelä SI

更新日期：1997-06-01 00:00:00