Abstract:
:Four different green fluorescent protein (GFP)-based whole-cell biosensors were created based on the DNA damage inducible SOS response of Escherichia coli in order to evaluate the sensitivity of individual SOS promoters toward genotoxic substances. Treatment with the known carcinogen N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) revealed that the promoter for the ColD plasmid-borne cda gene had responses 12, 5, and 3 times greater than the recA, sulA, and umuDC promoters, respectively, and also considerably higher sensitivity. Furthermore, we showed that when the SOS-GFP construct was introduced into an E. coli host deficient in the tolC gene, the minimal detection limits toward mitomycin C, MNNG, nalidixic acid, and formaldehyde were lowered to 9.1 nM, 0.16 microM, 1.1 microM, and 141 microM, respectively, which were two to six times lower than those in the wild-type strain. This study thus presents a new SOS-GFP whole-cell biosensor which is not only able to detect minute levels of genotoxins but, due to its use of the green fluorescent protein, also a reporter system which should be applicable in high-throughput screening assays as well as a wide variety of in situ detection studies.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Norman A,Hestbjerg Hansen L,Sørensen SJdoi
10.1128/AEM.71.5.2338-2346.2005keywords:
subject
Has Abstractpub_date
2005-05-01 00:00:00pages
2338-46issue
5eissn
0099-2240issn
1098-5336pii
71/5/2338journal_volume
71pub_type
杂志文章abstract::The analysis of the virulence determinants of phytopathogenic Rhodococcus fascians has been hampered by the lack of a system for introducing exogenous DNA. We investigated the possibility of genetic transformation of R. fascians by high-voltage electroporation of intact bacterial cells in the presence of plasmid DNA. ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.56.9.2818-2825.1990
更新日期:1990-09-01 00:00:00
abstract::Aldehydes are a class of chemicals with many industrial uses. Several aldehydes are responsible for flavors and fragrances present in plants, but aldehydes are not known to accumulate in most natural microorganisms. In many cases, microbial production of aldehydes presents an attractive alternative to extraction from ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章,评审
doi:10.1128/AEM.03319-14
更新日期:2015-03-01 00:00:00
abstract::[This corrects the article on p. 931 in vol. 46.]. ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.47.5.1193-1193.1984
更新日期:1984-05-01 00:00:00
abstract::[This corrects the article on p. 1239 in vol. 45.]. ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.46.1.296-296.1983
更新日期:1983-07-01 00:00:00
abstract::Three Rhizobium japonicum strains and two slow-growing cowpea-type Rhizobium strains were found to remain viable and able to rapidly modulate their respective hosts after being stored in purified water at ambient temperatures for periods of 1 year and longer. Three fast-growing Rhizobium species did not remain viable ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.47.5.895-900.1984
更新日期:1984-05-01 00:00:00
abstract::Brevibacterium linens M18, isolated from red smear cheese, produces a substance that inhibits the growth of Listeria spp. and several coryneform and other gram-positive bacteria. No gram-negative bacteria were inhibited. The substance is heat labile, sensitive to proteolytic enzymes, and stable between pH 3 and 12. Hi...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.60.10.3809-3814.1994
更新日期:1994-10-01 00:00:00
abstract::A polymerase chain reaction (PCR) method designed to sensitively detect and identify Campylobacter jejuni and Campylobacter coli without the need for isolating and culturing strains is described. The intergenic sequence between the flagellin genes flaA and flaB was amplified and characterized with a triple primer or s...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.59.7.2161-2165.1993
更新日期:1993-07-01 00:00:00
abstract::The composition of predominant soil bacteria during grassland succession was investigated in the Dutch Drentse A area. Five meadows, taken out of agricultural production at different time points, and one currently fertilized plot represented different stages of grassland succession. Since fertilization and agricultura...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.66.9.3998-4003.2000
更新日期:2000-09-01 00:00:00
abstract:UNLABELLED:By combining genomics and isotope imaging analysis using high-resolution secondary ion mass spectrometry (NanoSIMS), we examined the function and evolution of Bacteroidales ectosymbionts of the protist Barbulanympha from the hindguts of the wood-eating cockroach Cryptocercus punctulatus In particular, we inv...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00611-16
更新日期:2016-07-15 00:00:00
abstract::Marine microbial communities often contain multiple closely related phylogenetic clades, but in many cases, it is still unclear what physiological traits differentiate these putative ecotypes. The numerically abundant marine cyanobacterium Synechococcus can be divided into at least 14 clades. In order to better unders...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00358-06
更新日期:2006-11-01 00:00:00
abstract::The erythromycin resistance gene ermB has been found in a variety of gram-positive bacteria. This gene has also been found in Bacteroides species but only in six recently isolated strains; thus, the gene seems to have entered this genus only recently. One of the six Bacteroides ermB-containing isolates, WH207, could t...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.69.11.6455-6463.2003
更新日期:2003-11-01 00:00:00
abstract::Listeria monocytogenes can cause the serious infection listeriosis, which despite antibiotic treatment has a high mortality. Understanding the response of L. monocytogenes to antibiotic exposure is therefore important to ensure treatment success. Some bacteria survive antibiotic treatment by formation of persisters, w...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02184-13
更新日期:2013-12-01 00:00:00
abstract::The addition of positively charged colloidal materials to the growth medium markedly increased the concentration of cellulase enzymes produced by Trichoderma reesei Rut-C30. Filter paper activities of up to 4 and 13 IU/ml have been achieved by the addition of colloidal materials, using 3% lactose and 3% cellulose, res...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.49.4.934-938.1985
更新日期:1985-04-01 00:00:00
abstract::The human gut is host to a diversity of microorganisms, including the single-celled microbial eukaryote Blastocystis. Research has shown that most carriers host a single Blastocystis subtype (ST), which is unusual given the considerable within-host species diversity observed for other microbial genera in this ecosyste...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00520-15
更新日期:2015-06-15 00:00:00
abstract::Azospirillum spp. were shown to utilize both straw and xylan, a major component of straw, for growth with an adequate combined N supply and also under N-limiting conditions. For most strains examined, a semisolid agar medium was satisfactory, but several strains appeared to be capable of slow metabolism of the agar. S...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.49.2.423-428.1985
更新日期:1985-02-01 00:00:00
abstract::The routine production and storage of frozen doughs are still problematic. Although commercial baker's yeast is highly resistant to environmental stress conditions, it rapidly loses stress resistance during dough preparation due to the initiation of fermentation. As a result, the yeast loses gassing power significantl...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.68.10.4780-4787.2002
更新日期:2002-10-01 00:00:00
abstract::In this study, we compared different methods-including transmission electron microscopy-and various nucleic acid labeling methods in which we used the fluorochromes 4',6'-diamidino-2-phenylindole (DAPI), 4-[3-methyl-2,3-dihydro-(benzo-1, 3-oxazole)-2-methylmethyledene]-1-(3'-trimethyl ammoniumpropyl)-quinilinium diioi...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.66.6.2283-2289.2000
更新日期:2000-06-01 00:00:00
abstract::We investigated the genetic diversity and symbiotic efficiency of 223 Sinorhizobium sp. isolates sampled from a single Mediterranean soil and trapped with four Medicago truncatula lines. DNA molecular polymorphism was estimated by capillary electrophoresis-single-stranded conformation polymorphism and restriction frag...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01107-08
更新日期:2008-09-01 00:00:00
abstract::A total of 113 strains of mesophilic strains lactic streptococci of the species Streptococcus lactis, S. lactis subsp. diacetilactis, and S. cremoris, chosen from 291 strains that had been previously classified into six groups on the basis of their sensitivity to 132 virulent phages, were subjected to induction with m...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.43.2.349-356.1982
更新日期:1982-02-01 00:00:00
abstract::Here we describe novel forms of structural integration between endo- and episymbiotic microbes and an unusual new species of snail from hydrothermal vents in the Indian Ocean. The snail houses a dense population of gamma-proteobacteria within the cells of its greatly enlarged esophageal gland. This tissue setting diff...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.70.5.3082-3090.2004
更新日期:2004-05-01 00:00:00
abstract::Bacillus cereus endospores germinate in response to particular nutrients. Spores are able to sense these nutrients in the environment by receptors encoded by the gerA family of operons. Analysis of the Bacillus cereus ATCC 14579 genome revealed seven gerA family homologues. Using a transposon Tn917-based insertional m...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.71.2.774-781.2005
更新日期:2005-02-01 00:00:00
abstract::A field collected population of Plutella xylostella (SERD4) was selected in the laboratory with Bacillus thuringiensis endotoxins Cry1Ac (Cry1Ac-SEL) and Cry1Ab (Cry1Ab-SEL). Both subpopulations showed similar phenotypes: high resistance to the Cry1A toxins and little cross-resistance to Cry1Ca or Cry1D. A previous an...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.71.11.6863-6869.2005
更新日期:2005-11-01 00:00:00
abstract::Recombinant DNA clones carrying high-copy or low-copy sequences from Aspergillus nidulans and Neurospora crassa were used to identify restriction fragment length polymorphisms (RFLPs) diagnostic for members of the A. flavus group: A. flavus, A. parasiticus, and A. nomius. These fungi were resolved into three distinct ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.56.8.2453-2461.1990
更新日期:1990-08-01 00:00:00
abstract::[This corrects the article on p. 1580 in vol. 58.]. ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:
更新日期:1992-08-01 00:00:00
abstract::A molecular analysis of betaproteobacterial ammonia oxidizers and a N(2)O isotopomer analysis were conducted to study the sources of N(2)O emissions during the cow manure composting process. Much NO(2)(-)-N and NO(3)(-)-N and the Nitrosomonas europaea-like amoA gene were detected at the surface, especially at the top ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01394-09
更新日期:2010-03-01 00:00:00
abstract::Differences between the recombinant xylose-utilizing Saccharomyces cerevisiae strain TMB 3399 and the mutant strain TMB 3400, derived from TMB 3399 and displaying improved ability to utilize xylose, were investigated by using genome-wide expression analysis, physiological characterization, and biochemical assays. Samp...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.69.2.740-746.2003
更新日期:2003-02-01 00:00:00
abstract::Nontoxigenic strains of Clostridium botulinum types C and D are converted to toxigenic strains by infection with specific Tox+ bacteriophages. The nucleic acids were extracted from five converting phages, c-st, c-468, c-203, c-d6f, and d-1873, and one nonconverting phage, c-n71, and treated with nucleases. The nucleic...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.54.1.69-73.1988
更新日期:1988-01-01 00:00:00
abstract::The human intestine harbors both lactate-producing and lactate-utilizing bacteria. Lactate is normally present at <3 mmol liter(-1) in stool samples from healthy adults, but concentrations up to 100 mmol liter(-1) have been reported in gut disorders such as ulcerative colitis. The effect of different initial pH values...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00508-07
更新日期:2007-10-01 00:00:00
abstract::We describe here an improved method for isolating, purifying, and cloning DNA from diverse soil microbiota. Soil microorganisms were extracted from soils and embedded and lysed within an agarose plug. Nucleases that copurified with the metagenomic DNA were removed by incubating plugs with a high-salt and -formamide so...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02630-07
更新日期:2008-05-01 00:00:00
abstract::Polyhydroxyalkanoates (PHAs) are microbial polyesters that can be used as completely biodegradable polymers, but the high production cost prevents their use in a wide range of applications. Recombinant Escherichia coli strains harboring the Ralstonia eutropha PHA biosynthesis genes have been reported to have several a...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.64.12.4897-4903.1998
更新日期:1998-12-01 00:00:00