Abstract:
:The effects of mineral fertilizer (NPK) and organic manure on the community structure of soil ammonia-oxidizing bacteria (AOB) was investigated in a long-term (16-year) fertilizer experiment. The experiment included seven treatments: organic manure, half organic manure N plus half fertilizer N, fertilizer NPK, fertilizer NP, fertilizer NK, fertilizer PK, and the control (without fertilization). N fertilization greatly increased soil nitrification potential, and mineral N fertilizer had a greater impact than organic manure, while N deficiency treatment (PK) had no significant effect. AOB community structure was analyzed by PCR-denaturing gradient gel electrophoresis (PCR-DGGE) of the amoA gene, which encodes the alpha subunit of ammonia monooxygenase. DGGE profiles showed that the AOB community was more diverse in N-fertilized treatments than in the PK-fertilized treatment or the control, while one dominant band observed in the control could not be detected in any of the fertilized treatments. Phylogenetic analysis showed that the DGGE bands derived from N-fertilized treatments belonged to Nitrosospira cluster 3, indicating that N fertilization resulted in the dominance of Nitrosospira cluster 3 in soil. These results demonstrate that long-term application of N fertilizers could result in increased soil nitrification potential and the AOB community shifts in soil. Our results also showed the different effects of mineral fertilizer N versus organic manure N; the effects of P and K on the soil AOB community; and the importance of balanced fertilization with N, P, and K in promoting nitrification functions in arable soils.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Chu H,Fujii T,Morimoto S,Lin X,Yagi K,Hu J,Zhang Jdoi
10.1128/AEM.01536-06subject
Has Abstractpub_date
2007-01-01 00:00:00pages
485-91issue
2eissn
0099-2240issn
1098-5336pii
AEM.01536-06journal_volume
73pub_type
杂志文章abstract::The S85 type strain of Fibrobacter succinogenes, a major ruminal fibrolytic species, was isolated 49 years ago from a bovine rumen and has been used since then as a model for extensive studies. To assess the validity of this model, we compared the cellulase- and xylanase-degrading activities of several other F. succin...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.70.4.2172-2179.2004
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journal_title:Applied and environmental microbiology
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doi:10.1128/AEM.62.4.1378-1382.1996
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journal_title:Applied and environmental microbiology
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pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Applied and environmental microbiology
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doi:10.1128/AEM.53.9.2021-2025.1987
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更新日期:1984-06-01 00:00:00
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pub_type: 杂志文章
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journal_title:Applied and environmental microbiology
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更新日期:1993-10-01 00:00:00
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更新日期:2001-03-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.57.4.918-923.1991
更新日期:1991-04-01 00:00:00
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pub_type: 杂志文章
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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更新日期:2009-08-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.61.8.3177-3179.1995
更新日期:1995-08-01 00:00:00