Reduction of Norwalk virus, poliovirus 1, and bacteriophage MS2 by ozone disinfection of water.

abstract：:Analysis of electron spin resonance spectra of 5-doxyl stearic acid in aqueous suspensions of Clostridium acetobutylicum ATCC 824 and the butanol-tolerant SA-2 derivative during a small-scale fermentation at three different butanol challenge levels indicated that the SA-2 strain is able to respond to the physical flui...

journal_title：Applied and environmental microbiology

authors： Baer SH,Bryant DL,Blaschek HP

更新日期：1989-10-01 00:00:00

abstract：:The nprA gene, encoding Bacillus thuringiensis neutral protease A, was cloned by the use of gene-specific oligonucleotides. The size of neutral protease A deduced from the nprA sequence was 566 amino acids (60,982 Da). The cloned nprA gene was partially deleted in vitro, and the deleted allele, designated nprA3, was u...

journal_title：Applied and environmental microbiology

authors： Donovan WP,Tan Y,Slaney AC

更新日期：1997-06-01 00:00:00

abstract：:There are a number of genetic tools available for studying Francisella tularensis, the etiological agent of tularemia; however, there is no effective inducible or repressible gene expression system. Here, we describe inducible and repressible gene expression systems for F. tularensis based on the Tet repressor, TetR. ...

journal_title：Applied and environmental microbiology

authors： LoVullo ED,Miller CN,Pavelka MS Jr,Kawula TH

更新日期：2012-10-01 00:00:00

abstract：:Petuely's selective medium for Bifidobacterium was improved by addition of riboflavin, nucleic acid bases, pyruvic acid, and nalidixic acid. The modified medium, when examined under strictly anaerobic conditions for efficient isolation of Bifidobacterium from human fecal samples, exhibited selective and high viable co...

journal_title：Applied and environmental microbiology

authors： Tanaka R,Mutai M

更新日期：1980-11-01 00:00:00

abstract：:Pseudomonas oleovorans is capable of producing poly(3-hydroxyalkanoates) (PHAs) as intracellular storage material. To analyze the possible involvement of phaD in medium-chain-length (MCL) PHA biosynthesis, we generated a phaD knockout mutant by homologous recombination. Upon disruption of the phaD gene, MCL PHA polyme...

journal_title：Applied and environmental microbiology

authors： Klinke S,de Roo G,Witholt B,Kessler B

更新日期：2000-09-01 00:00:00

abstract：:The goal of this field study was to provide insight into three distinct populations of microorganisms involved in in situ metabolism of phenol. Our approach measured 13CO2 respired from [13C]phenol and stable isotope probing (SIP) of soil DNA at an agricultural field site. Traditionally, SIP-based investigations have ...

journal_title：Applied and environmental microbiology

authors： DeRito CM,Pumphrey GM,Madsen EL

更新日期：2005-12-01 00:00:00

abstract：:A European baseline survey during the years 2005 and 2006 has revealed that the monophasic Salmonella enterica subsp. enterica serovar 4,12:d:- was, with a prevalence of 23.6%, the most frequently isolated serovar in German broiler flocks. In Denmark and the United Kingdom, its serovar prevalences were 15.15% and 2.8%...

journal_title：Applied and environmental microbiology

authors： Huehn S,Bunge C,Junker E,Helmuth R,Malorny B

更新日期：2009-02-01 00:00:00

abstract：:Cyanuric acid is an industrial chemical produced during the biodegradation of s-triazine pesticides. The biodegradation of cyanuric acid has been elucidated using a single model system, Pseudomonas sp. strain ADP, in which cyanuric acid hydrolase (AtzD) opens the s-triazine ring and AtzEG deaminates the ring-opened pr...

journal_title：Applied and environmental microbiology

authors： Aukema KG,Tassoulas LJ,Robinson SL,Konopatski JF,Bygd MD,Wackett LP

更新日期：2020-01-07 00:00:00

abstract：:Higher yields of sterigmatocystin were obtained with Aspergillus versicolor than with Bipolaris sorokiniana both in liquid and on solid media. The optimum temperature for sterigmatocystin production by A. versicolor was 27 to 29 degrees C and 23 degrees C for B. sorokiniana. In liquid shake cultures, production of ste...

journal_title：Applied and environmental microbiology

authors： Rabie CJ,Lubben A,Steyn M

更新日期：1976-08-01 00:00:00

abstract：:Nine immunoglobulin G and nine immunoglobulin M murine monoclonal antibody-producing hybridomas reactive with live Salmonella bacteria were obtained from several fusions of immune spleen cells and Sp2/0 myeloma cells. The antibodies were selected by the magnetic immunoluminescence assay. The monoclonal antibodies were...

journal_title：Applied and environmental microbiology

authors： Torensma R,Visser MJ,Aarsman CJ,Poppelier MJ,van Beurden R,Fluit AC,Verhoef J

更新日期：1992-12-01 00:00:00

abstract：:Phormium yellow leaf (PYL) phytoplasma causes a lethal disease of the monocotyledon, New Zealand flax (Phormium tenax). The 16S rRNA genes of PYL phytoplasma were amplified from infected flax by PCR and cloned, and the nucleotide sequences were determined. DNA sequencing and Southern hybridization analysis of genomic ...

journal_title：Applied and environmental microbiology

authors： Liefting LW,Andersen MT,Beever RE,Gardner RC,Forster RL

更新日期：1996-09-01 00:00:00

abstract：:Streptomyces exfoliatus SMF19 produced two types of extracellular beta-lactamase-inhibiting proteins, 48 (beta LIP-I) and 33 (beta LIP-II) kDa. The inhibition mode of beta LIP-I on Bacto Penase and benzylpenicillin as substrates was uncompetitive (i.e., both Km and Vmax were changed), while that of beta LIP-II was non...

journal_title：Applied and environmental microbiology

authors： Kim MK,Lee KJ

更新日期：1994-03-01 00:00:00

abstract：:The environmental transport of Cryptosporidium spp. through combined sewer overflow (CSO) and the occurrence of several emerging human-pathogenic Cryptosporidium species in developing countries remain unclear. In this study, we collected 40 CSO samples and 40 raw wastewater samples from Shanghai, China, and examined t...

journal_title：Applied and environmental microbiology

authors： Huang C,Hu Y,Wang L,Wang Y,Li N,Guo Y,Feng Y,Xiao L

更新日期：2017-08-01 00:00:00

abstract：:Micro-Raman spectroscopy is a fast and sensitive tool for the detection, classification, and identification of biological organisms. The vibrational spectrum inherently serves as a fingerprint of the biochemical composition of each bacterium and thus makes identification at the species level, or even the subspecies le...

journal_title：Applied and environmental microbiology

authors： Stöckel S,Schumacher W,Meisel S,Elschner M,Rösch P,Popp J

更新日期：2010-05-01 00:00:00

abstract：:Thymidine incorporation by the microbial community on standing dead leaves of Spartina alterniflora did not obey many of the assumptions inherent in the use of the technique in planktonic systems. Incorporation rates of [methly-H]thymidine were nonsaturable over a wide concentration range (10 to 10 nM). Owing to metab...

journal_title：Applied and environmental microbiology

authors： Fallon RD,Newell SY

更新日期：1986-11-01 00:00:00

abstract：:Submerged cultures of Penicillium urticae (NRRL 2159A) produced the antibiotics patulin and griseofulvin when grown in a glucose-nitrate medium. A high concentration of calcium (i.e., 68 mM) inhibited the production of both antibiotics while stimulating conidiogenesis. Conidial mutants that were defective in an early ...

journal_title：Applied and environmental microbiology

authors： Sekiguchi J,Gaucher GM

更新日期：1977-01-01 00:00:00

abstract：:In the natural environment, most of the phages that target bacteria are thought to exist in biofilm ecosystems. The purpose of this study was to gain a clearer understanding of the reactivity of these viral particles when they come into contact with bacteria embedded in biofilms. Experimentally, we quantified lactococ...

journal_title：Applied and environmental microbiology

authors： Briandet R,Lacroix-Gueu P,Renault M,Lecart S,Meylheuc T,Bidnenko E,Steenkeste K,Bellon-Fontaine MN,Fontaine-Aupart MP

更新日期：2008-04-01 00:00:00

abstract：:An organism capable of growth on pyridine was isolated from soil by enrichment culture techniques and identified as Micrococcus luteus. The organism oxidized pyridine for energy and released N contained in the pyridine ring as ammonium. The organism could not grow on mono- or disubstituted pyridinecarboxylic acids or ...

journal_title：Applied and environmental microbiology

authors： Sims GK,Sommers LE,Konopka A

更新日期：1986-05-01 00:00:00

abstract：:A sublethal dose of ethanol (5%, vol/vol), acid (HCl, pH 4.5 to 5.0), H2O2 (500 ppm), or NaCl (7%, wt/vol) was added to a Listeria monocytogenes culture at the exponential phase, and the cells were allowed to grow for 1 h. Exponential-phase cells also were heat shocked at 45 degrees C for 1 h. The stress-adapted cells...

journal_title：Applied and environmental microbiology

authors： Lou Y,Yousef AE

更新日期：1997-04-01 00:00:00

abstract：:As a result of the recently proposed mandatory groundwater disinfection requirements to inactivate viruses in potable water supplies, there has been increasing interest in virus fate and transport in the subsurface. Several models have been developed to predict the fate of viruses in groundwater, but few include trans...

journal_title：Applied and environmental microbiology

authors： Yates MV,Ouyang Y

更新日期：1992-05-01 00:00:00

abstract：:An assembly consisting of Styrofoam cups with membranes of varying porosities was developed to study microorganism-root interactions. The assembly permitted uniform distribution of a bacterium in soil and was simple, easy to use, and disposable. In tests with the bacterium Pseudomonas solanacearum, little difference i...

journal_title：Applied and environmental microbiology

authors： Hartel PG,Billingsley JW,Williamson JW

更新日期：1989-05-01 00:00:00

abstract：:Phytoplankton replace phosphorus-containing lipids (P-lipids) with non-P analogues, boosting growth in P-limited oceans. In the model diatom Thalassiosira pseudonana, the substitution dynamics of lipid headgroups are well described, but those of the individual lipids, differing in fatty acid composition, are unknown. ...

journal_title：Applied and environmental microbiology

authors： Hunter JE,Brandsma J,Dymond MK,Koster G,Moore CM,Postle AD,Mills RA,Attard GS

更新日期：2018-03-01 00:00:00

abstract：:The exopolysaccharides (EPSs) produced by some bacteria are potential growth substrates for other bacteria in soil. We used stable-isotope probing (SIP) to identify aerobic soil bacteria that assimilated the cellulose produced by Gluconacetobacter xylinus or the EPS produced by Beijerinckia indica. The latter is a het...

journal_title：Applied and environmental microbiology

authors： Wang X,Sharp CE,Jones GM,Grasby SE,Brady AL,Dunfield PF

更新日期：2015-07-01 00:00:00

abstract：:Protein S is a major spore coat protein of Myxococcus xanthus, consisting of two homologous domains, the N-terminal domain (NTD) and the C-terminal domain, both of which contain a Ca(2+)-binding site. Protein S tightly binds to myxospores in a Ca(2+)-dependent manner. Here, we constructed a novel expression vector, pC...

journal_title：Applied and environmental microbiology

authors： Kobayashi H,Yoshida T,Inouye M

更新日期：2009-08-01 00:00:00

abstract：:A total of 765 Escherichia coli isolates from point and nonpoint sources were collected from the Apalachicola National Estuarine Research Reserve, and their multiple-antibiotic-resistance (MAR) profiles were determined with 10 antibiotics. E. coli isolates from point sources showed significantly greater resistance (P ...

journal_title：Applied and environmental microbiology

authors： Parveen S,Murphree RL,Edmiston L,Kaspar CW,Portier KM,Tamplin ML

更新日期：1997-07-01 00:00:00

abstract：:Rubber-degrading activity was found in the extracellular culture medium of Xanthomonas sp. strain 35Y which was grown on natural rubber latex. Natural rubber in the latex state was degraded by the crude enzyme, and two fractions were separately observed by gel permeation chromatography of the reaction products. One fr...

journal_title：Applied and environmental microbiology

authors： Tsuchii A,Takeda K

更新日期：1990-01-01 00:00:00

abstract：:The effects of aflatoxin on egg production, fertility, hatchability, and progeny performance were investigated by feeding dietary aflatoxin at dose levels of 0,5, and 10 mug/g to mature broiler breeder hens for 4 weeks. Sixteen hens were used for each dietary dose level. Egg production decreased significantly during w...

journal_title：Applied and environmental microbiology

authors： Howarth B Jr,Wyatt RD

更新日期：1976-05-01 00:00:00

abstract：:Lactobacillus gasseri ATCC 33323, Lactobacillus salivarius subsp. salivarius UCC 118, and Lactobacillus casei ATCC 334 contain one (LgaI), four (Sal1, Sal2, Sal3, Sal4), and one (Lca1) distinguishable prophage sequences, respectively. Sequence analysis revealed that LgaI, Lca1, Sal1, and Sal2 prophages belong to the g...

journal_title：Applied and environmental microbiology

authors： Ventura M,Canchaya C,Bernini V,Altermann E,Barrangou R,McGrath S,Claesson MJ,Li Y,Leahy S,Walker CD,Zink R,Neviani E,Steele J,Broadbent J,Klaenhammer TR,Fitzgerald GF,O'toole PW,van Sinderen D

更新日期：2006-05-01 00:00:00

abstract：:The axy43A gene encoding the intracellular trifunctional xylanolytic enzyme from Paenibacillus curdlanolyticus B-6 was cloned and expressed in Escherichia coli Recombinant PcAxy43A consisting of a glycoside hydrolase family 43 and a family 6 carbohydrate-binding module exhibited endo-xylanase, β-xylosidase, and arabin...

journal_title：Applied and environmental microbiology

authors： Teeravivattanakit T,Baramee S,Phitsuwan P,Waeonukul R,Pason P,Tachaapaikoon C,Sakka K,Ratanakhanokchai K

更新日期：2016-12-01 00:00:00

abstract：:Eight concentration and purification methods were evaluated to determine percentages of recovery of Cryptosporidium parvum oocysts from calf feces. The NaCl flotation method generally resulted in the highest percentages of recovery. Based on the percentages of recovery, the amounts of fecal debris in the final oocyst ...

journal_title：Applied and environmental microbiology

authors： Kuczynska E,Shelton DR

更新日期：1999-07-01 00:00:00