Abstract:
:A total of 765 Escherichia coli isolates from point and nonpoint sources were collected from the Apalachicola National Estuarine Research Reserve, and their multiple-antibiotic-resistance (MAR) profiles were determined with 10 antibiotics. E. coli isolates from point sources showed significantly greater resistance (P < 0.05) to antibiotics and higher MAR indices than isolates from nonpoint sources. Specifically, 65 different resistance patterns were observed among point source isolates, compared to 32 among nonpoint source isolates. Examples of this contrast in MAR profiles included percentages of isolates with resistance to chlortetracycline-sulfathiazole of 33.7% and to chlortetracycline-penicillin G-sulfathiazole of 14.5% for point source isolates versus 15.4 and 1.7%, respectively, for nonpoint source isolates. MAR profile homology, based on coefficient similarity, showed that isolates from point sources were markedly more diverse than isolates from nonpoint sources. Seven clusters were observed among point source isolates, with a coefficient value of approximately 1.8. In contrast, only four clusters were observed among nonpoint source isolates. Covariance matrices of data displayed six very distinct foci representing nonpoint source E. coli isolates. Importantly, E. coli isolates obtained directly from human and animal feces also clustered among point and nonpoint sources, respectively. We conclude that E. coli MAR profiles were associated with point and nonpoint sources of pollution within Apalachicola Bay and that this method may be useful in facilitating management of other estuaries.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Parveen S,Murphree RL,Edmiston L,Kaspar CW,Portier KM,Tamplin MLdoi
10.1128/AEM.63.7.2607-2612.1997subject
Has Abstractpub_date
1997-07-01 00:00:00pages
2607-12issue
7eissn
0099-2240issn
1098-5336journal_volume
63pub_type
杂志文章abstract::DNA primers corresponding to conserved motifs in bacterial repetitive (REP, ERIC, and BOX) elements and PCR were used to show that REP-, ERIC-, and BOX-like DNA sequences are widely distributed in phytopathogenic Xanthomonas and Pseudomonas strains. REP-, ERIC, and BOX-PCR (collectively known as rep-PCR) were used to ...
journal_title:Applied and environmental microbiology
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doi:10.1128/AEM.60.7.2286-2295.1994
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journal_title:Applied and environmental microbiology
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doi:10.1128/aem.67.10.4901-4907.2001
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doi:10.1128/AEM.61.2.617-622.1995
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doi:10.1128/AEM.37.1.122-126.1979
更新日期:1979-01-01 00:00:00
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doi:10.1128/AEM.47.4.879-881.1984
更新日期:1984-04-01 00:00:00
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doi:10.1128/AEM.60.4.1347-1352.1994
更新日期:1994-04-01 00:00:00
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doi:10.1128/AEM.53.12.2840-2843.1987
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pub_type: 杂志文章
doi:10.1128/AEM.64.7.2361-2366.1998
更新日期:1998-07-01 00:00:00
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doi:10.1128/AEM.35.4.631-635.1978
更新日期:1978-04-01 00:00:00
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更新日期:1989-09-01 00:00:00
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journal_title:Applied and environmental microbiology
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.46.1.98-105.1983
更新日期:1983-07-01 00:00:00
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journal_title:Applied and environmental microbiology
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doi:10.1128/AEM.62.7.2636-2640.1996
更新日期:1996-07-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.32.3.441-442.1976
更新日期:1976-09-01 00:00:00