Abstract:
:A membrane filtration method incorporating a combination of anaerobic and aerobic incubation has been developed for the enumeration of Aeromonas spp. in drinking water. The use of anaerobic incubation improved the detection of Aeromonas spp. by reducing the growth of nonaeromonads. The confirmation rate of presumptive Aeromonas spp. identified on the initial isolation agar exceeded 92%.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Cunliffe DA,Adcock Pdoi
10.1128/AEM.55.9.2138-2140.1989subject
Has Abstractpub_date
1989-09-01 00:00:00pages
2138-40issue
9eissn
0099-2240issn
1098-5336journal_volume
55pub_type
杂志文章abstract::Procedures based on DNA hybridization and PCR were developed for quality control of Rhizobium inoculants. Inoculants for pea and goat's rue were produced by Elomestari Ltd., Juva, Finland, in sterile dry fine peat by the standard procedure used by the company. The inoculants contained Rhizobium galegae HAMBI 1174 and ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.61.5.1822-1827.1995
更新日期:1995-05-01 00:00:00
abstract::Light microscopy and a radioassay detected no significant difference in adherence of Kanagawa-positive and Kanagawa-negative strains of Vibrio parahaemolyticus to human epithelial cell lines. ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.39.2.369-371.1980
更新日期:1980-02-01 00:00:00
abstract::Bacillus thuringiensis vegetative insecticidal proteins (Vip3A) have been recently introduced in important crops as a strategy to delay the emerging resistance to the existing Cry toxins. The mode of action of Vip3A proteins has been studied in Spodoptera frugiperda with the aim of characterizing their binding to the ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01521-14
更新日期:2014-10-01 00:00:00
abstract::In soil, Acidobacteria constitute on average 20% of all bacteria, are highly diverse, and are physiologically active in situ. However, their individual functions and interactions with higher taxa in soil are still unknown. Here, potential effects of land use, soil properties, plant diversity, and soil nanofauna on aci...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01325-12
更新日期:2012-10-01 00:00:00
abstract:UNLABELLED:Mechanistic studies of many processes in Agrobacterium tumefaciens have been hampered by a lack of genetic tools for characterization of essential genes. In this study, we used a Tn7-based method for inducible control of transcription from an engineered site on the chromosome. We demonstrate that this method...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01392-16
更新日期:2016-07-29 00:00:00
abstract::We constructed a 7.9-kilobase-pair recombinant shuttle plasmid, designated pHR106, by combining desired segments of three plasmids: an Escherichia coli plasmid (pSL100) which provides a multiple cloning site, a Clostridium perfringens plasmid (pJU122) which provides a clostridial origin of replication, and an E. coli ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.54.1.268-270.1988
更新日期:1988-01-01 00:00:00
abstract::Flavobacterium psychrophilum, a member of the Cytophaga-Flavobacterium-Bacteroides group, is an important pathogen of salmonid fish. Previous attempts to develop genetic techniques for this fastidious, psychrotrophic bacterium have met with failure. Here we describe the development of techniques for the genetic manipu...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.70.1.581-587.2004
更新日期:2004-01-01 00:00:00
abstract::The aim of this study was to investigate the phenotypic and genotypic antibiotic resistance profiles of pseudomonads isolated from surfaces of a goat and lamb slaughterhouse, which were representative of areas that are possible sources of meat contamination. Mesophilic (85 isolates) and psychrotrophic (37 isolates) ps...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01998-14
更新日期:2014-11-01 00:00:00
abstract::Spores of Clostridium bifermentans KMR-1 were produced for use as a microbial inoculum for bioremediation and were preserved in both liquid and dry forms. All spore formulations showed good viability and ability to biodegrade the target compound, 2,4,6-trinitrotoluene (TNT), after 4 months of storage. For low-cost bul...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.63.5.2100-2104.1997
更新日期:1997-05-01 00:00:00
abstract::This work presents data on the application of a bacterial luciferase used to monitor gene expression of Streptococcus thermophilus in the digestive tract. The main result is that the bacterium was able to produce an active beta-galactosidase in the digestive tract, although it did not multiply during its transit. This...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.68.2.938-941.2002
更新日期:2002-02-01 00:00:00
abstract::The human oral cavity is a complex ecosystem, and the alterations in salivary microbial communities are associated with both oral and nonoral diseases. The Mediterranean diet (MD) is a healthy dietary pattern useful for both prevention and treatment of several diseases. To further explore the effects of the MD on huma...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00777-20
更新日期:2020-06-02 00:00:00
abstract::The pediocin-like bacteriocins, produced by lactic acid bacteria, are bactericidal polypeptides with very similar primary structures. Peptide synthesis followed by reverse-phase and ion-exchange chromatographies yielded biologically active pediocin-like bacteriocins in amounts and with a purity sufficient for characte...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.62.9.3313-3318.1996
更新日期:1996-09-01 00:00:00
abstract::The D-values of Bacillus pumilus spores on various devices ranged from 0.14 to 0.23 Mrads. The majority of devices displayed D-values equal to or less than the value obtained on filter paper. Increased resistivity was also encountered. ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.36.2.392-393.1978
更新日期:1978-08-01 00:00:00
abstract::Flocs consisting of Anabaena and Zoogloea spp. were used as a model system for the study of planktonic phototroph-heterotroph interactions. In CO(2)-limited continuous culture (3.2 mumol of NaHCO(3) liter h, 1.5 mumol of glucose liter h, pH 8.5, D = 0.026 h), the biomass of the phototroph increased 8.6-fold due to ass...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.44.1.84-87.1982
更新日期:1982-07-01 00:00:00
abstract::This work reports the utilization of an in vivo expression technology system to identify in vivo-induced (ivi) genes in Yersinia ruckeri after determination of the conditions needed for its selection in fish. Fourteen clones were selected, and the cloned DNA fragments were analyzed after partial sequencing. In additio...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.70.9.5199-5207.2004
更新日期:2004-09-01 00:00:00
abstract::This study quantified the levels of airborne microorganisms in six swine farms with more than 10,000 pigs in subtropical Taiwan. We evaluated breeding, growing, and finishing stalls, which were primarily open-air buildings, as well as partially enclosed farrowing and nursery piggeries. Airborne culturable bacteria, gr...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.67.1.155-161.2001
更新日期:2001-01-01 00:00:00
abstract::The activity of glutaraldehyde (GTA) at low concentrations (less than 2%) against poliovirus was assessed by a suspension procedure. The inactivation kinetics showed that concentrations of less than or equal to 0.10% were effective against purified poliovirus at pH 7.2; a 1 log10 reduction was obtained in 70 min with ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.57.4.1156-1160.1991
更新日期:1991-04-01 00:00:00
abstract::A molecular method based on restriction fragment length polymorphism (RFLP) of PCR-amplified fragments of the 23S rRNA gene was designed to rapidly identify Listeria strains to the species level. Two fragments (S1, 460 bp, and S2, 890 bp) were amplified from boiled DNA. S2 was cut with the restriction enzymes XmnI or ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.69.11.6386-6392.2003
更新日期:2003-11-01 00:00:00
abstract::Diversity among 130 strains of Bacillus polymyxa was studied; the bacteria were isolated by immunotrapping from nonrhizosphere soil (32 strains), rhizosphere soil (38 strains), and the rhizoplane (60 strains) of wheat plantlets growing in a growth chamber. The strains were characterized phenotypically by 63 auxanograp...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.58.6.1894-1903.1992
更新日期:1992-06-01 00:00:00
abstract::The reduction of cytotoxicity in virus concentrates from environmental samples was accomplished by high-speed centrifugation and by filtration of the samples through positively charged depth filters. ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.43.3.731-733.1982
更新日期:1982-03-01 00:00:00
abstract::Seasonal variation in the phylogenetic composition of Synechococcus assemblages in estuarine and coastal waters of Hong Kong was examined through pyrosequencing of the rpoC1 gene. Sixteen samples were collected in 2009 from two stations representing estuarine and ocean-influenced coastal waters, respectively. Synechoc...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01895-15
更新日期:2015-11-01 00:00:00
abstract::Flavobacteria are a poorly understood and speciated group of commensal bacteria and opportunistic pathogens. The psychrotroph Flavobacterium psychrophilum is the etiological agent of rainbow trout fry syndrome and bacterial cold water disease, septicemic diseases that heavily impact salmonids. Consequently, two verifi...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.67.2.750-759.2001
更新日期:2001-02-01 00:00:00
abstract::The bacterial communities associated with the development of necroses in injured agria cactus tissue were examined in the field by using both human-induced injuries and cactus tissue inoculated with cactophilic bacteria. Whole-cell bacterial fatty acids were used to determine when and where each of 23 detected species...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.60.2.619-625.1994
更新日期:1994-02-01 00:00:00
abstract::The genetic diversity of Borrelia burgdorferi sensu lato was assessed in a focus of Lyme borreliosis in southern Britain dominated by game birds. Ticks, rodents, and pheasants were analyzed for spirochete infections by PCR-targeting the 23S-5S rRNA genes, followed by genotyping by the reverse line blot method. In ques...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.64.4.1169-1174.1998
更新日期:1998-04-01 00:00:00
abstract::This is the first study to characterize the environmental conditions which contribute to the presence and proliferation of environmental mycobacteria in a major freshwater river. Over 20 different species of environmental mycobacteria were isolated, including the pathogenic M. avium and M. kansasii. Species of the rap...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.71.10.5719-5727.2005
更新日期:2005-10-01 00:00:00
abstract::Thirty deoxynivalenol-producing F. culmorum strains, isolated from wheat grains, were incubated in vitro and analyzed for trichothecene production. Seventeen strains produced more than 1 ppm of deoxynivalenol and acetyldeoxynivalenol and were considered high-deoxynivalenol-producing strains, whereas 13 F. culmorum str...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.68.11.5472-5479.2002
更新日期:2002-11-01 00:00:00
abstract::Many people in Japan often detect an unpleasant odor generated from laundry that is hung to dry indoors or when using their already-dried laundry. Such an odor is often described as a "wet-and-dirty-dustcloth-like malodor" or an "acidic or sweaty odor." In this study, we isolated the major microorganisms associated wi...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.07816-11
更新日期:2012-05-01 00:00:00
abstract::Aflatoxin B1 in a 5-micrograms/ml water solution was sensitive to ionizing radiation. Inactivation was assayed by the Ames microsome mutagenicity test and confirmed by thin-layer chromatography. Destruction of aflatoxin B1 had already begun at 2.5 kilograms (kGy; 1 kGy = 0.1 Mrad), but a dose exceeding 10 kGy was nece...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.43.6.1317-1319.1982
更新日期:1982-06-01 00:00:00
abstract::Four different green fluorescent protein (GFP)-based whole-cell biosensors were created based on the DNA damage inducible SOS response of Escherichia coli in order to evaluate the sensitivity of individual SOS promoters toward genotoxic substances. Treatment with the known carcinogen N-methyl-N'-nitro-N-nitrosoguanidi...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.71.5.2338-2346.2005
更新日期:2005-05-01 00:00:00
abstract::A mutant of Pseudomonas sp. strain HBP1, originally isolated on 2-hydroxybiphenyl, was selected for the ability to grow on 2-propylphenol as the sole carbon and energy source. In the mutant strain, which was designated as Pseudomonas sp. strain HBP1 Prp, the cellular induction mechanism involved in the synthesis of th...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.59.3.860-866.1993
更新日期:1993-03-01 00:00:00