Variation in resistance to high hydrostatic pressure and rpoS heterogeneity in natural isolates of Escherichia coli O157:H7.

Abstract:

:Several natural isolates of Escherichia coli O157:H7 have previously been shown to exhibit stationary-phase-dependent variation in their resistance to inactivation by high hydrostatic pressure. In this report we demonstrate that loss of the stationary-phase-inducible sigma factor RpoS resulted in decreased resistance to pressure in E. coli O157:H7 and in a commensal strain. Furthermore, variation in the RpoS activity of the natural isolates of O157:H7 correlated with the pressure resistance of those strains. Heterogeneity was noted in the rpoS alleles of the natural isolates that may explain the differences in RpoS activity. These results are consistent with a role for rpoS in mediating resistance to high hydrostatic pressure in E. coli O157:H7.

journal_name

Appl Environ Microbiol

authors

Robey M,Benito A,Hutson RH,Pascual C,Park SF,Mackey BM

doi

10.1128/aem.67.10.4901-4907.2001

keywords:

subject

Has Abstract

pub_date

2001-10-01 00:00:00

pages

4901-7

issue

10

eissn

0099-2240

issn

1098-5336

journal_volume

67

pub_type

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