Localization of active microorganisms in cheese by autoradiography.

abstract：:A defined substrate method was developed to simultaneously enumerate total coliforms and Escherichia coli from drinking waters without the need for confirmatory or completed tests. It is a new method based on technology that uses a hydrolyzable substrate as a specific indicator-nutrient for the target microbes. No equ...

journal_title：Applied and environmental microbiology

authors： Edberg SC,Allen MJ,Smith DB

更新日期：1988-06-01 00:00:00

abstract：:Ring-hydroxylating dioxygenases (RHDs) play a crucial role in the biodegradation of a range of aromatic hydrocarbons found on polluted sites, including polycyclic aromatic hydrocarbons (PAHs). Current knowledge on RHDs comes essentially from studies on culturable bacterial strains, while compelling evidence indicates ...

journal_title：Applied and environmental microbiology

authors： Chemerys A,Pelletier E,Cruaud C,Martin F,Violet F,Jouanneau Y

更新日期：2014-11-01 00:00:00

abstract：:The lantibiotic (lanthionine-containing antibiotic) mersacidin is an antimicrobial peptide consisting of 20 amino acids and is produced by Bacillus sp. strain HIL Y-85,54728. The structural gene (mrsA) and the genes for producer self-protection, modification enzymes, transport proteins, and regulator proteins are orga...

journal_title：Applied and environmental microbiology

authors： Schmitz S,Hoffmann A,Szekat C,Rudd B,Bierbaum G

更新日期：2006-11-01 00:00:00

abstract：:Most associations between animals and their gut microbiota are dynamic, involving sustained transfer of food-associated microbial cells into the gut and shedding of microorganisms into the external environment with feces, but the interacting effects of host and microbial factors on the composition of the internal and ...

journal_title：Applied and environmental microbiology

authors： Wong AC,Luo Y,Jing X,Franzenburg S,Bost A,Douglas AE

更新日期：2015-09-01 00:00:00

abstract：:Ten phenols were selected as natural laccase mediators after screening 44 different compounds with a recalcitrant dye (Reactive Black 5) as a substrate. Their performances were evaluated at different mediator/dye ratios and incubation times (up to 6 h) by the use of Pycnoporus cinnabarinus and Trametes villosa laccase...

journal_title：Applied and environmental microbiology

authors： Camarero S,Ibarra D,Martínez MJ,Martínez AT

更新日期：2005-04-01 00:00:00

abstract：:Gamma irradiation is a nonthermal processing technology that has been used for the preservation of a variety of food products. This technology has been shown to effectively inactivate bacterial pathogens. Currently, the FDA has approved doses of up to 4.0 kGy to control food-borne pathogens in fresh iceberg lettuce an...

journal_title：Applied and environmental microbiology

authors： Feng K,Divers E,Ma Y,Li J

更新日期：2011-05-01 00:00:00

abstract：:The function of small secreted proteins (SSPs) in saprotrophic fungi is, for the most part, unknown. The white-rot mushroom Pleurotus ostreatus produces considerable amounts of SSPs at the onset of secondary metabolism, during colony development, and in response to chemical compounds such as 5-hydroxymethylfurfural an...

journal_title：Applied and environmental microbiology

authors： Feldman D,Amedi N,Carmeli S,Yarden O,Hadar Y

更新日期：2019-07-18 00:00:00

abstract：:The natural products of both eremofortin C (EC) and PR toxin are secondary metabolites of Penicillium roqueforti. Because the chemical structures of EC and PR toxin are closely related to each other and differ only by a hydroxyl functional group in EC and an aldehyde functional group in PR toxin at the C-12 position, ...

journal_title：Applied and environmental microbiology

authors： Li SY,Chang SC,Wei RD

更新日期：1985-09-01 00:00:00

abstract：:The extremely thermoacidophilic archaeon Metallosphaera sedula (optimum growth temperature, 73°C, pH 2.0) grows chemolithoautotrophically on metal sulfides or molecular hydrogen by employing the 3-hydroxypropionate/4-hydroxybutyrate (3HP/4HB) carbon fixation cycle. This cycle adds two CO2 molecules to acetyl coenzyme ...

journal_title：Applied and environmental microbiology

authors： Hawkins AB,Adams MW,Kelly RM

更新日期：2014-04-01 00:00:00

abstract：:Among 1,962 bacterial isolates from a smear-surface soft cheese (Munster cheese) screened for activity against Listeria monocytogenes, six produced antilisterial compounds other than organic acids. The bacterial strain WHE 92, which displayed the strongest antilisterial effect, was identified at the DNA level as Lacto...

journal_title：Applied and environmental microbiology

authors： Ennahar S,Aoude-Werner D,Sorokine O,Van Dorsselaer A,Bringel F,Hubert JC,Hasselmann C

更新日期：1996-12-01 00:00:00

abstract：:The esterases and lipases from the α/β hydrolase superfamily exhibit an enormous sequence diversity, fold plasticity, and activities. Here, we present the comprehensive sequence and biochemical analyses of seven distinct esterases and lipases from the metagenome of Lake Arreo, an evaporite karstic lake in Spain (42°46...

journal_title：Applied and environmental microbiology

authors： Martínez-Martínez M,Alcaide M,Tchigvintsev A,Reva O,Polaina J,Bargiela R,Guazzaroni ME,Chicote A,Canet A,Valero F,Rico Eguizabal E,Guerrero Mdel C,Yakunin AF,Ferrer M

更新日期：2013-06-01 00:00:00

abstract：:The phospholipid fatty acid (PLFA) pattern was analyzed in a forest humus and in an arable soil experimentally polluted with Cd, Cu, Ni, Pb, or Zn at different concentrations. In both soil types, there were gradual changes in the PLFA patterns for the different levels of metal contamination. The changes in the forest ...

journal_title：Applied and environmental microbiology

authors： Frostegård A,Tunlid A,Bååth E

更新日期：1993-11-01 00:00:00

abstract：:The bphC and bphD genes of Pseudomonas putida involved in the catabolism of polychlorinated biphenyls or biphenyl were identified, localized, and studied for expression in Escherichia coli. This was achieved by cloning a 2.4-kilobase (kb) DNA fragment of recombinant cosmid pOH101 into HindIII site of pUC plasmids down...

journal_title：Applied and environmental microbiology

authors： Khan AA,Walia SK

更新日期：1990-04-01 00:00:00

abstract：:We examined physiological adaptations which allow the psychrotroph Rhodococcus sp. strain Q15 to assimilate alkanes at a low temperature (alkanes are contaminants which are generally insoluble and/or solid at low temperatures). During growth at 5 degrees C on hexadecane or diesel fuel, strain Q15 produced a cell surfa...

journal_title：Applied and environmental microbiology

authors： Whyte LG,Slagman SJ,Pietrantonio F,Bourbonnière L,Koval SF,Lawrence JR,Inniss WE,Greer CW

更新日期：1999-07-01 00:00:00

abstract：:The Amazon rainforest, the largest equatorial forest in the world, is being cleared for pasture and agricultural use at alarming rates. Tropical deforestation is known to cause alterations in microbial communities at taxonomic and phylogenetic levels, but it is unclear whether microbial functional groups are altered. ...

journal_title：Applied and environmental microbiology

authors： Mirza BS,Potisap C,Nüsslein K,Bohannan BJ,Rodrigues JL

更新日期：2014-01-01 00:00:00

abstract：:A genome-wide search was performed to identify simple sequence repeat (SSR) loci among the available sequence databases from four strains of Xylella fastidiosa (strains causing Pierce's disease, citrus variegated chlorosis, almond leaf scorch, and oleander leaf scorch). Thirty-four SSR loci were selected for SSR prime...

journal_title：Applied and environmental microbiology

authors： Lin H,Civerolo EL,Hu R,Barros S,Francis M,Walker MA

更新日期：2005-08-01 00:00:00

abstract：:Chromohalobacter salexigens DSM 3043 can grow on N,N-dimethylglycine (DMG) as the sole C, N, and energy source and utilize sarcosine as the sole N source under aerobic conditions. However, little is known about the genes and enzymes involved in the conversion of DMG to sarcosine in this strain. In the present study, g...

journal_title：Applied and environmental microbiology

authors： Yang T,Shao YH,Guo LZ,Meng XL,Yu H,Lu WD

更新日期：2020-08-18 00:00:00

abstract：:An immunoblot procedure for the strain-specific quantitative analysis of commercial Rhizobium inoculants was developed. The technique greatly reduced the time required for inoculant analysis. Correlation between immunoblot analysis and traditional plant nodule grow-out most-probable-number techniques was r = 0.90 for ...

journal_title：Applied and environmental microbiology

authors： Olsen PE,Rice WA

更新日期：1989-02-01 00:00:00

abstract：:Eukaryotes may influence pollutant degradation processes in groundwater ecosystems by activities such as predation on bacteria and recycling of nutrients. Culture-independent community profiling and phylogenetic analysis of 18S rRNA gene fragments, as well as culturing, were employed to obtain insight into the sedimen...

journal_title：Applied and environmental microbiology

authors： Brad T,Braster M,van Breukelen BM,van Straalen NM,Röling WF

更新日期：2008-07-01 00:00:00

abstract：:A biologically active metabolite was found in crude extracts of Curvularia lunata (Wakker) Boedijn (ATCC 34690) isolated from decayed tissues of litchi fruit (Litchi chinensis Sonn.). The fungus was grown on a shredded wheat-yeast extract-sucrose medium, and cultures were extracted with chloroform after 3 weeks of gro...

journal_title：Applied and environmental microbiology

authors： Wells JM,Cole RJ,Cutler HC,Spalding DH

更新日期：1981-04-01 00:00:00

abstract：:Veratryl alcohol, added as a supplement to cultures of Phanerochaete chrysosporium, enhanced ligninase activity through protection of the ligninase against inactivation by hydrogen peroxide produced by this fungus in cultures. In the presence of veratryl alcohol, the loss of ligninase activity observed in non-protein-...

journal_title：Applied and environmental microbiology

authors： Tonon F,Odier E

更新日期：1988-02-01 00:00:00

abstract：:Epilithic bacteria were isolated nonselectively from riverbed stones and examined by gel zymography for their ability to produce alkylsulfatase (AS) enzymes and thus to metabolize alkyl sulfate surfactants such as sodium dodecyl sulfate. The percentages of AS+ isolates from stone epilithon at five sites from the sourc...

journal_title：Applied and environmental microbiology

authors： Anderson DJ,Day MJ,Russell NJ,White GF

更新日期：1988-02-01 00:00:00

abstract：:We investigated long-chain fatty acid (LCFA)-degrading anaerobic microbes by enrichment, isolation, and RNA-based stable isotope probing (SIP). Primary enrichment cultures were made with each of four LCFA substrates (palmitate, stearate, oleate, or linoleate, as the sole energy source) at 55 degrees C or 37 degrees C ...

journal_title：Applied and environmental microbiology

authors： Hatamoto M,Imachi H,Ohashi A,Harada H

更新日期：2007-02-01 00:00:00

abstract：:Bacterial plasmolytic response to osmotic stress was evaluated as a physical indicator of membrane integrity and hence cellular viability. Digital image analysis and either low-magnification dark-field, high-magnification phase-contrast, or confocal laser microscopy, in conjunction with pulse application of a 1.5 M Na...

journal_title：Applied and environmental microbiology

authors： Korber DR,Choi A,Wolfaardt GM,Caldwell DE

更新日期：1996-11-01 00:00:00

abstract：:The growth of and production of protease, alpha-amylase, alpha-galactosidase, and lipase by Actinomucor taiwanensis in relation to temperature and relative humidity during the preparation of sufu (Chinese cheese) pehtze were investigated. The incubation temperature, humidity, and cultivation time greatly affected the ...

journal_title：Applied and environmental microbiology

authors： Chou CC,Ho FM,Tsai CS

更新日期：1988-03-01 00:00:00

abstract：:Selectively regulating gene expression is an essential molecular tool that is lacking for many pathogenic gram-positive bacteria. In this report, we describe the evaluation of a series of promoters regulated by the bacteriophage P1 temperature-sensitive C1 repressor in Enterococcus faecium, Enterococcus faecalis, and ...

journal_title：Applied and environmental microbiology

authors： Schofield DA,Westwater C,Hoel BD,Werner PA,Norris JS,Schmidt MG

更新日期：2003-06-01 00:00:00

abstract：:In order to elucidate interactions between sulfate reduction and dechlorination, we systematically evaluated the effects of different concentrations of sulfate and sulfide on reductive dechlorination by isolates, constructed consortia, and enrichments containing Dehalococcoides sp. Sulfate (up to 5 mM) did not inhibit...

journal_title：Applied and environmental microbiology

authors： Mao X,Polasko A,Alvarez-Cohen L

更新日期：2017-03-31 00:00:00

abstract：:In soil, Acidobacteria constitute on average 20% of all bacteria, are highly diverse, and are physiologically active in situ. However, their individual functions and interactions with higher taxa in soil are still unknown. Here, potential effects of land use, soil properties, plant diversity, and soil nanofauna on aci...

journal_title：Applied and environmental microbiology

authors： Naether A,Foesel BU,Naegele V,Wüst PK,Weinert J,Bonkowski M,Alt F,Oelmann Y,Polle A,Lohaus G,Gockel S,Hemp A,Kalko EK,Linsenmair KE,Pfeiffer S,Renner S,Schöning I,Weisser WW,Wells K,Fischer M,Overmann J,Friedric

更新日期：2012-10-01 00:00:00

abstract：:The gut microbiota plays important roles in its host. However, how each microbiota member contributes to the behavior of the whole population is not known. In this study, we therefore determined protein expression in the cecal microbiota in chickens of selected ages and in 7-day-old chickens inoculated with different ...

journal_title：Applied and environmental microbiology

authors： Polansky O,Sekelova Z,Faldynova M,Sebkova A,Sisak F,Rychlik I

更新日期：2015-12-28 00:00:00

abstract：:Chromosomal integration of heterologous metabolic pathways is optimal for industrially relevant fermentation, as plasmid-based fermentation causes extra metabolic burden and genetic instabilities. In this work, chromosomal integration was adapted for the production of mevalonate, which can be readily converted into β-...

journal_title：Applied and environmental microbiology

authors： Wang J,Niyompanich S,Tai YS,Wang J,Bai W,Mahida P,Gao T,Zhang K

更新日期：2016-11-21 00:00:00