Eukaryotic diversity in an anaerobic aquifer polluted with landfill leachate.

abstract：:The rapid degradation of marine infrastructure at the low tide level due to accelerated low-water corrosion (ALWC) is a problem encountered worldwide. Despite this, there is limited understanding of the microbial communities involved in this process. We obtained samples of the orange-colored tubercles commonly associa...

journal_title：Applied and environmental microbiology

authors： Phan HC,Wade SA,Blackall LL

更新日期：2020-06-17 00:00:00

abstract：:Terminal olefins (1-alkenes) are natural products that have important industrial applications as both fuels and chemicals. However, their biosynthesis has been largely unexplored. We describe a group of bacteria, Jeotgalicoccus spp., which synthesize terminal olefins, in particular 18-methyl-1-nonadecene and 17-methyl...

journal_title：Applied and environmental microbiology

authors： Rude MA,Baron TS,Brubaker S,Alibhai M,Del Cardayre SB,Schirmer A

更新日期：2011-03-01 00:00:00

abstract：:The strictly anaerobic pathogenic bacterium Clostridium difficile occurs in the human gut and is able to thrive from fermentation of leucine. Thereby the amino acid is both oxidized to isovalerate plus CO(2) and reduced to isocaproate. In the reductive branch of this pathway, the dehydration of (R)-2-hydroxyisocaproyl...

journal_title：Applied and environmental microbiology

authors： Kim J,Darley D,Selmer T,Buckel W

更新日期：2006-09-01 00:00:00

abstract：:Sludges derived from wastewater treatment are foul-smelling, biologically unstable substances. As well as containing numerous pathogenic microorganisms, they also consist of organic matter that can be used as agricultural fertilizer. Legislation nevertheless requires sludges to be virologically tested prior to spreadi...

journal_title：Applied and environmental microbiology

authors： Monpoeho S,Maul A,Bonnin C,Patria L,Ranarijaona S,Billaudel S,Ferré V

更新日期：2004-09-01 00:00:00

abstract：:Thirty-three enteric isolates from Australian (Escherichia coli only) and United Kingdom (U.K.) (Salmonella sp., Citrobacter spp., and E. coli) piggeries were characterized with respect to their copper resistance. The copper resistance phenotypes of four new Australian E. coli isolates were comparable with that of the...

journal_title：Applied and environmental microbiology

authors： Williams JR,Morgan AG,Rouch DA,Brown NL,Lee BT

更新日期：1993-08-01 00:00:00

abstract：:We devised a new method (the dorayaki method) using marine agar under in situ pressures to isolate barophilic bacteria from the intestinal contents of three deep-sea fishes (two Coryphaenoides yaquinae samples and one Ilyophis sp. sample) retrieved from depths of 4,700 to 6,100 m in the Northwest Pacific Ocean. All 10...

journal_title：Applied and environmental microbiology

authors： Nakayama A,Yano Y,Yoshida K

更新日期：1994-11-01 00:00:00

abstract：:Germlings of Botrytis cinerea, an important fungal pathogen of plants, produce an extracellular matrix (ECM), or ensheathing film, that serves, in part, in their attachment (R. P. Doss, et al., Appl. Environ. Microbiol. 61:260-265, 1995). The composition of this film has been ascertained by using samples obtained by g...

journal_title：Applied and environmental microbiology

authors： Doss RP

更新日期：1999-02-01 00:00:00

abstract：:Biotransformation plays an increasingly important role in the industrial production of fine chemicals due to its high product specificity and low energy requirement. One challenge in biotransformation is the toxicity of substrates and/or products to biocatalytic microorganisms and enzymes. Biofilms are known for their...

journal_title：Applied and environmental microbiology

authors： Li XZ,Webb JS,Kjelleberg S,Rosche B

更新日期：2006-02-01 00:00:00

abstract：:Thymidine incorporation by the microbial community on standing dead leaves of Spartina alterniflora did not obey many of the assumptions inherent in the use of the technique in planktonic systems. Incorporation rates of [methly-H]thymidine were nonsaturable over a wide concentration range (10 to 10 nM). Owing to metab...

journal_title：Applied and environmental microbiology

authors： Fallon RD,Newell SY

更新日期：1986-11-01 00:00:00

abstract：:Poultry are considered the major reservoir for Campylobacter jejuni, a leading bacterial cause of human food-borne diarrhea. To understand the ecology of C. jejuni and develop strategies to control C. jejuni infection in the animal reservoir, we initiated studies to examine the potential role of anti-Campylobacter mat...

journal_title：Applied and environmental microbiology

authors： Sahin O,Zhang Q,Meitzler JC,Harr BS,Morishita TY,Mohan R

更新日期：2001-09-01 00:00:00

abstract：:Two duck farms in Hong Kong were examined monthly for 1 year for the occurrence and persistence of influenza viruses within the duck communities. The predominant virus in one community was H3N2, a virus antigenically related to the pandemic Hong Kong strain. This virus was isolated monthly throughout the year from fec...

journal_title：Applied and environmental microbiology

authors： Markwell DD,Shortridge KF

更新日期：1982-01-01 00:00:00

abstract：:Our understanding of leptospiral pathogenesis, which remains poorly understood, depends on reliable genetic tools for functional analysis of genes in pathogenic strains. In this study, we report the first demonstration of conjugation between Escherichia coli and Leptospira spp. by using RP4 derivative conjugative plas...

journal_title：Applied and environmental microbiology

authors： Picardeau M

更新日期：2008-01-01 00:00:00

abstract：:Wastewater disinfection is used in many countries for reducing fecal coliform levels in effluents. Disinfection is therefore frequently used to improve recreational bathing waters which do not comply with microbiological standards. It is unknown whether human enteric viruses (which are responsible for waterborne disea...

journal_title：Applied and environmental microbiology

authors： Tree JA,Adams MR,Lees DN

更新日期：2003-04-01 00:00:00

abstract：:We describe useful vectors to select double-crossover events directly in site-directed marker exchange mutagenesis in gram-negative bacteria. These vectors contain the gusA marker gene, providing colorimetric screens to identify bacteria harboring those sequences. The applicability of these vectors was shown by mappin...

journal_title：Applied and environmental microbiology

authors： Katzen F,Becker A,Ielmini MV,Oddo CG,Ielpi L

更新日期：1999-01-01 00:00:00

abstract：:For efficient generation of high-affinity protein-based binding molecules, fast and reliable downstream characterization platforms are needed. In this work, we have explored the use of staphylococcal cell surface display together with flow cytometry for affinity characterization of candidate affibody molecules directl...

journal_title：Applied and environmental microbiology

authors： Löfblom J,Sandberg J,Wernérus H,Ståhl S

更新日期：2007-11-01 00:00:00

abstract：:Subterranean clover plants possessing two equally infectible and robust lateral root systems ("split roots") were used in conjunction with several specific mutant strains (derived from Rhizobium trifolii ANU843) to investigate a systemic plant response induced by infective Rhizobium strains. This plant response contro...

journal_title：Applied and environmental microbiology

authors： Sargent L,Huang SZ,Rolfe BG,Djordjevic MA

更新日期：1987-07-01 00:00:00

abstract：:The production of a stable cDNA copy of an unstable RNA molecule by reverse transcription is a widely used and essential technology for many important applications, such as the construction of gene libraries, production of DNA probes, and analysis of gene expression by reverse transcriptase PCR (RT-PCR). However, the ...

journal_title：Applied and environmental microbiology

authors： Vellore J,Moretz SE,Lampson BC

更新日期：2004-12-01 00:00:00

abstract：:A gene that codes for a novel intracellular poly(3-hydroxybutyrate) (PHB) depolymerase, designated PhaZ1, has been identified in the genome of Bacillus megaterium. A native PHB (nPHB) granule-binding assay showed that purified soluble PhaZ1 had strong affinity for nPHB granules. Turbidimetric analyses revealed that Ph...

journal_title：Applied and environmental microbiology

authors： Chen HJ,Pan SC,Shaw GC

更新日期：2009-08-01 00:00:00

abstract：:Cultures of Anabaena variabilis were exposed to different light intensities, and the time course of photoadaptation was measured by photosynthetic rate and changes in pigmentation. A shift down in intensity of 284 muEin . m . sec caused a temporary decrease in the photosynthetic response followed by gradual adaptation...

journal_title：Applied and environmental microbiology

authors： Collins CD,Boylen CW

更新日期：1982-07-01 00:00:00

abstract：:The single glucosyltransferase (GTF) of Streptococcus gordonii Challis CH1 makes alpha 1,3- and alpha 1,6-linked glucans from sucrose. The GTF carboxyl-terminal region has six direct repeats thought to be involved in glucan binding. Strains with defined mutations in this region have been described recently (M. M. Vick...

journal_title：Applied and environmental microbiology

authors： Vickerman MM,Clewell DB

更新日期：1997-05-01 00:00:00

abstract：:Here, we report a novel method to produce microencapsulated enzymes using Saccharomyces cerevisiae spores. In sporulating cells, soluble secreted proteins are transported to the spore wall. Previous work has shown that the spore wall is capable of retaining soluble proteins because its outer layers work as a diffusion...

journal_title：Applied and environmental microbiology

authors： Shi L,Li Z,Tachikawa H,Gao XD,Nakanishi H

更新日期：2014-08-01 00:00:00

abstract：:Effects of the sesquiterpene farnesol (3,7,11-trimethyl-2,6,10-dodecatrien-1-ol) and the sesquiterpene derivative geosmin (1,10-trans-dimethyl-trans-9-decalol) were investigated in a geosmin-producing actinomycete, Streptomyces tendae. Exposure to 300 microM farnesol reduced biomass (fresh matter) accumulation by 97% ...

journal_title：Applied and environmental microbiology

authors： Dionigi CP,Millie DF,Johnsen PB

更新日期：1991-12-01 00:00:00

abstract：:A TAT peptide-delivered molecular beacon was developed and utilized to enumerate murine norovirus 1, a human norovirus (NoV) surrogate, in RAW 264.7 cells. This allowed the detection of a single infective virus within 6 h, a 12-fold improvement in time required for viral detection and quantification compared to that r...

journal_title：Applied and environmental microbiology

authors： Ganguli PS,Chen W,Yates MV

更新日期：2011-08-01 00:00:00

abstract：:Fluctuations in ciliated protozoan concentrations were monitored in 40 individually fed crossbred heifers that were stepped up to an 85% concentrate diet either slowly (12 days) or rapidly (3 days), with or without monensin (30 ppm). Ruminal fluid was withdrawn from all animals by stomach tube at the start of the stud...

journal_title：Applied and environmental microbiology

authors： Towne G,Nagaraja TG,Brandt RT Jr,Kemp KE

更新日期：1990-10-01 00:00:00

abstract：:Stored sugarbeet roots began fermenting within 24 h after oxygen was depleted at 26 C when the resident bacterial populations increased dramatically. Most of the bacteria present after anaerobic storage for 7 days at 26 C could hydrolyze sucrose in vitro. Although pH and sucrose levels decreased and invert sugar level...

journal_title：Applied and environmental microbiology

authors： Cole DF,Bugbee WM

更新日期：1976-05-01 00:00:00

abstract：:Ethylbenzene dehydrogenase (EbDH) catalyzes the initial step in anaerobic degradation of ethylbenzene in denitrifying bacteria, namely, the oxygen-independent hydroxylation of ethylbenzene to (S)-1-phenylethanol. In our study we investigate the kinetic properties of 46 substrate analogs acting as substrates or inhibit...

journal_title：Applied and environmental microbiology

authors： Knack D,Hagel C,Szaleniec M,Dudzik A,Salwinski A,Heider J

更新日期：2012-09-01 00:00:00

abstract：:Very little is known about the interaction of bryophytes with bacteria. Therefore, we analyzed bacteria associated with three bryophyte species, Tortula ruralis, Aulacomnium palustre, and Sphagnum rubellum, which represent typical moss species of three nutrient-poor plant communities at the southern Baltic Sea coast i...

journal_title：Applied and environmental microbiology

authors： Opelt K,Berg G

更新日期：2004-11-01 00:00:00

abstract：:Strain-specific monoclonal antibodies (MAbs) were developed for three different bacterial isolates obtained from a freshwater environment (Lake Plusssee) in the spring of 1990. The three isolates, which were identified by molecular methods, were as follows: Cytophaga johnsonae PX62, Comamonas acidovorans PX54, and Aer...

journal_title：Applied and environmental microbiology

authors： Faude UC,Höfle MG

更新日期：1997-11-01 00:00:00

abstract：:An experiment was conducted with subsurface sediments from Oak Ridge National Laboratory to determine the potential for reduction of U(VI) under sulfate-reducing conditions with either ethanol or acetate as the electron donor. The results showed extensive U(VI) reduction in sediments supplied with either electron dono...

journal_title：Applied and environmental microbiology

authors： Converse BJ,Wu T,Findlay RH,Roden EE

更新日期：2013-07-01 00:00:00

abstract：:Batch cultures of a lithotrophic Fe(II)-oxidizing bacterium, strain BrT, isolated from the rhizosphere of a wetland plant, were grown in bioreactors and used to determine the significance of microbial Fe(II) oxidation at circumneutral pH and to identify abiotic variables that affect the partitioning between microbial ...

journal_title：Applied and environmental microbiology

authors： Neubauer SC,Emerson D,Megonigal JP

更新日期：2002-08-01 00:00:00