Clearance of human-pathogenic viruses from sludge: study of four stabilization processes by real-time reverse transcription-PCR and cell culture.

abstract：:Of the toxins produced by Bacillus cereus, the emetic toxin is likely the most dangerous but, due to the lack of a suitable assay, the least well known. In this paper, a new, sensitive, inexpensive, and rapid bioassay for detection of the emetic toxin of B. cereus is described. The assay is based on the loss of motili...

journal_title：Applied and environmental microbiology

authors： Andersson MA,Mikkola R,Helin J,Andersson MC,Salkinoja-Salonen M

更新日期：1998-04-01 00:00:00

abstract：:Volume 61, no. 4, p. 1211, column 2, line 40: this sentence should read as follows. "The viable-culture methods for Legionella sp. detection, however, often fail, and the Centers for Disease Control and Prevention has turned, when necessary, to PCR for epidemiological investigations of Legionnaires' disease and Pontia...

journal_title：Applied and environmental microbiology

authors： Atlas RM,Williams JF,Huntington MK

更新日期：1996-04-01 00:00:00

abstract：:We studied the growth of six culturable bacterial lineages from coastal North Sea picoplankton in environmental samples under different incubation conditions. The grazing pressure of heterotrophic nanoflagellates (HNF) was reduced either by double prefiltration through 0.8- micro m-pore-size filters or by 10-fold dilu...

journal_title：Applied and environmental microbiology

authors： Beardsley C,Pernthaler J,Wosniok W,Amann R

更新日期：2003-05-01 00:00:00

abstract：:Upon exposure of Nitrosomonas europaea to chloroform (7 microM, 1 h), transcripts for 175 of 2,460 genes were found at higher levels in treated cells than in untreated cells and transcripts for 501 genes were found at lower levels. With chloromethane (3.2 mM, 1 h), transcripts for 67 genes were at higher levels and tr...

journal_title：Applied and environmental microbiology

authors： Gvakharia BO,Permina EA,Gelfand MS,Bottomley PJ,Sayavedra-Soto LA,Arp DJ

更新日期：2007-05-01 00:00:00

abstract：:Biofilm bacteria challenged with monochloramine retained significant respiratory activity, even though they could not be cultured on agar plates. Microbial colony counts on agar media declined by approximately 99.9% after 1 h of disinfection, whereas the number of bacteria stained by a fluorescent redox dye experience...

journal_title：Applied and environmental microbiology

authors： Stewart PS,Griebe T,Srinivasan R,Chen CI,Yu FP,deBeer D,McFeters GA

更新日期：1994-05-01 00:00:00

abstract：:High-cell-density fermentation for industrial production of chemicals can impose numerous stresses on cells due to high substrate, product, and by-product concentrations; high osmolarity; reactive oxygen species; and elevated temperatures. There is a need to develop platform strains of industrial microorganisms that a...

journal_title：Applied and environmental microbiology

authors： Lennen RM,Herrgård MJ

更新日期：2014-10-01 00:00:00

abstract：:Rates of dissolved inorganic carbon (DIC), formate, and acetate mineralization and/or assimilation were determined in 13 high-temperature (>73 °C) hot springs in Yellowstone National Park (YNP), Wyoming, in order to evaluate the relative importance of these substrates in supporting microbial metabolism. While 9 of the...

journal_title：Applied and environmental microbiology

authors： Urschel MR,Kubo MD,Hoehler TM,Peters JW,Boyd ES

更新日期：2015-06-01 00:00:00

abstract：:Thirty-one urease-positive thermophilic Campylobacter (UPTC) isolates, including three reference strains (NCTC12892, NCTC12895 and NCTC12896), and three Campylobacter lari isolates, which were isolated from several countries and sources, were compared genotypically by using multilocus enzyme electrophoresis (MLEE). We...

journal_title：Applied and environmental microbiology

authors： Matsuda M,Kaneko A,Stanley T,Millar BC,Miyajima M,Murphy PG,Moore JE

更新日期：2003-06-01 00:00:00

abstract：:The isophthalate (IPA) degradation gene cluster (iphACBDR) responsible for the conversion of IPA into protocatechuate (PCA) was isolated from Comamonas sp. strain E6, which utilizes phthalate isomers as sole carbon and energy sources via the PCA 4,5-cleavage pathway. Based on amino acid sequence similarity, the iphA, ...

journal_title：Applied and environmental microbiology

authors： Fukuhara Y,Inakazu K,Kodama N,Kamimura N,Kasai D,Katayama Y,Fukuda M,Masai E

更新日期：2010-01-01 00:00:00

abstract：:Thermal inactivation of desiccation-adapted Salmonella spp. in aged chicken litter was investigated in comparison with that in a nonadapted control to examine potential cross-tolerance of desiccation-adapted cells to heat treatment. A mixture of four Salmonella serovars was inoculated into the finished compost with 20...

journal_title：Applied and environmental microbiology

authors： Chen Z,Diao J,Dharmasena M,Ionita C,Jiang X,Rieck J

更新日期：2013-11-01 00:00:00

abstract：:Severe intoxications caused by the Bacillus cereus emetic toxin cereulide can hardly be prevented due to the ubiquitous distribution and heat resistance of spores and the extreme thermal and chemical stability of cereulide. It would therefore be desirable to inhibit cereulide synthesis during food manufacturing proces...

journal_title：Applied and environmental microbiology

authors： Frenzel E,Letzel T,Scherer S,Ehling-Schulz M

更新日期：2011-02-01 00:00:00

abstract：:An amylosucrase gene was subjected to high-rate segmental random mutagenesis, which was directed toward a segment encoding amino acids that influence the interaction with substrate molecules in subsites -1 to +3. A screen was used to identify enzyme variants with compromised glucan chain elongation. With an average mu...

journal_title：Applied and environmental microbiology

authors： Schneider J,Fricke C,Overwin H,Hofmann B,Hofer B

更新日期：2009-12-01 00:00:00

abstract：:The obligate intracellular growth of Rickettsia prowazekii places severe restrictions on the analysis of rickettsial gene expression. With a small genome, predicted to code for 835 proteins, identifying which proteins are differentially expressed in rickettsiae that are isolated from different hosts or that vary in vi...

journal_title：Applied and environmental microbiology

authors： Tucker AM,Driskell LO,Pannell LK,Wood DO

更新日期：2011-07-01 00:00:00

abstract：:A basal (BC) medium devoid of added carbohydrates, a complete (CC) medium containing nine carbohydrates were developed for enumerating rumen bacteria. The colony counts on the BC medium were 85 to 100% of those obtained on the CC medium. These colonies were pinpoint size (less than or equal to mm in diameter) but incr...

journal_title：Applied and environmental microbiology

authors： Leedle JA,Hespell RB

更新日期：1980-04-01 00:00:00

abstract：:During incubation of seawater in bottles, the decrease in dissolved oxygen is often nonlinear over time scales frequently used to measure respiration. Numbers of bacteria always increase, and rates of assimilation of dissolved leucine often increase exponentially. This suggests that sample handling disrupts the previo...

journal_title：Applied and environmental microbiology

authors： Pomeroy LR,Sheldon JE,Sheldon WM

更新日期：1994-01-01 00:00:00

abstract：:Methanobacterium espanolae, an acidiphilic methanogen, required acetate for maximal growth on H(2)-CO(2). In the presence of 5 to 15 mM acetate, at a growth pH of 5.5, the mu(max) was 0.05 h. M. espanolae consumed 12.3 mM acetate during 96 h of incubation at 35 degrees C with shaking at 100 rpm. At initial acetate lev...

journal_title：Applied and environmental microbiology

authors： Patel GB,Sprott D,Ekiel I

更新日期：1993-04-01 00:00:00

abstract：:An enrichment method using d-cycloserine was designed for the isolation of spontaneous mutants of Zymomonas mobilis deficient in glucose or fructose utilization. The mutants could easily be isolated since they represented 80 to 90% of the population after two and three enrichment cycles. Glucokinase and fructokinase a...

journal_title：Applied and environmental microbiology

authors： Ait-Abdelkader N,Pencreach G,Joset F,Baratti JC

更新日期：1996-03-01 00:00:00

abstract：:An important feature of microbial biofilms is the development of four-dimensional physical and chemical gradients in space and time. There is need for novel approaches to probe these so-called microenvironments to determine their effect on biofilm-specific processes. In this study, we describe the use of seminaphthorh...

journal_title：Applied and environmental microbiology

authors： Hunter RC,Beveridge TJ

更新日期：2005-05-01 00:00:00

abstract：:Bacteria in the genus Streptomyces and its close relatives are prolific producers of secondary metabolites with antibiotic activity. Genome sequencing of these bacteria has revealed a rich source of potentially new antibiotic pathways, whose products have never been observed. Moreover, these new pathways can provide n...

journal_title：Applied and environmental microbiology

authors： Fayed B,Ashford DA,Hashem AM,Amin MA,El Gazayerly ON,Gregory MA,Smith MC

更新日期：2015-12-01 00:00:00

abstract：:Medicinal plants are valuable resources of natural antimicrobial materials. A novel small protein with antimicrobial activities, designated LJAMP1, was purified from the seeds of a medicinal herb, motherwort (Leonurus japonicus Houtt). LJAMP1 is a heat-stable protein with a molecular mass of 7.8 kDa and a determined i...

journal_title：Applied and environmental microbiology

authors： Yang X,Xiao Y,Wang X,Pei Y

更新日期：2007-02-01 00:00:00

abstract：:The U.S. Environmental Protection Agency's information collection rule requires the use of 1MDS electropositive filters for concentrating enteric viruses from water, but unfortunately, these filters are not cost-effective for routine viral monitoring. In this study, an inexpensive electropositive cartridge filter, the...

journal_title：Applied and environmental microbiology

authors： Karim MR,Rhodes ER,Brinkman N,Wymer L,Fout GS

更新日期：2009-04-01 00:00:00

abstract：:Streptococcus agalactiae (group B Streptococcus [GBS]) is a cause of severe infections, particularly during the newborn period. While methods exist for generating chromosomal mutations in GBS, they are cumbersome and inefficient and present significant challenges if the goal is to study subtle mutations, such as singl...

journal_title：Applied and environmental microbiology

authors： Hooven TA,Bonakdar M,Chamby AB,Ratner AJ

更新日期：2019-03-22 00:00:00

abstract：:Flavobacteria are a poorly understood and speciated group of commensal bacteria and opportunistic pathogens. The psychrotroph Flavobacterium psychrophilum is the etiological agent of rainbow trout fry syndrome and bacterial cold water disease, septicemic diseases that heavily impact salmonids. Consequently, two verifi...

journal_title：Applied and environmental microbiology

authors： Crump EM,Perry MB,Clouthier SC,Kay WW

更新日期：2001-02-01 00:00:00

abstract：:The transport characteristics of two adhesion-deficient, indigenous groundwater strains, Comamonas sp. strain DA001 and Erwinia herbicola OYS2-A, were studied by using intact sediment cores (7 by 50 cm) from Oyster, Va. Both strains are gram-negative rods (1.10 by 0.56 and 1.56 by 0.46 microm, respectively) with stron...

journal_title：Applied and environmental microbiology

authors： Dong H,Rothmel R,Onstott TC,Fuller ME,DeFlaun MF,Streger SH,Dunlap R,Fletcher M

更新日期：2002-05-01 00:00:00

abstract：:Pseudomonas putida F1 and Pseudomonas sp. strain JS150 initiate toluene degradation by incorporating molecular oxygen into the aromatic nucleus to form cis-1,2-dihydroxy-3-methylcyclohexa-3,5-diene. When toluene-grown cells were incubated with 2- and 3-nitrotoluene, the major products identified were 2- and 3-nitroben...

journal_title：Applied and environmental microbiology

authors： Robertson JB,Spain JC,Haddock JD,Gibson DT

更新日期：1992-08-01 00:00:00

abstract：:To assess the extent of genotypic and phenotypic diversity within species of purple nonsulfur bacteria found in aquatic sediments, a total of 128 strains were directly isolated from agar plates that had been inoculated with sediment samples from Haren and De Biesbosch in The Netherlands. All isolates were initially ch...

journal_title：Applied and environmental microbiology

authors： Oda Y,Wanders W,Huisman LA,Meijer WG,Gottschal JC,Forney LJ

更新日期：2002-07-01 00:00:00

abstract：:Novel strains of obligately chemolithoautotrophic, sulfur-oxidizing bacteria have been isolated from various depths of Lake Fryxell, Antarctica. Physiological, morphological, and phylogenetic analyses showed these strains to be related to mesophilic Thiobacillus species, such as T. thioparus. However, the psychrotoler...

journal_title：Applied and environmental microbiology

authors： Sattley WM,Madigan MT

更新日期：2006-08-01 00:00:00

abstract：:Characteristics of an obligately methylotrophic coccoid methanogen (strain GS-16) previously isolated from estuarine sediment are described. Growth was demonstrated on dimethyl sulfide (DMS) or trimethylamine (TMA), but not on methane thiol, methane thiol plus hydrogen, dimethyl disulfide, or methionine. DMS-grown cel...

journal_title：Applied and environmental microbiology

authors： Oremland RS,Kiene RP,Mathrani I,Whiticar MJ,Boone DR

更新日期：1989-04-01 00:00:00

abstract：:Submicronic fungal fragments have been observed in in vitro aerosolization experiments. The occurrence of these particles has therefore been suggested to contribute to respiratory health problems observed in mold-contaminated indoor environments. However, the role of submicronic fragments in exacerbating adverse healt...

journal_title：Applied and environmental microbiology

authors： Afanou KA,Straumfors A,Skogstad A,Nayak AP,Skaar I,Hjeljord L,Tronsmo A,Eduard W,Green BJ

更新日期：2015-09-01 00:00:00

abstract：:Through genome-wide transcript analysis of a reference strain and two recombinant Saccharomyces cerevisiae strains with different rates of galactose uptake, we obtained information about the global transcriptional response to metabolic engineering of the GAL gene regulatory network. One of the recombinant strains over...

journal_title：Applied and environmental microbiology

authors： Bro C,Knudsen S,Regenberg B,Olsson L,Nielsen J

更新日期：2005-11-01 00:00:00