Abstract:
:An enrichment method using d-cycloserine was designed for the isolation of spontaneous mutants of Zymomonas mobilis deficient in glucose or fructose utilization. The mutants could easily be isolated since they represented 80 to 90% of the population after two and three enrichment cycles. Glucokinase and fructokinase activities in the mutants were affected.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Ait-Abdelkader N,Pencreach G,Joset F,Baratti JCdoi
10.1128/AEM.62.3.1096-1098.1996keywords:
subject
Has Abstractpub_date
1996-03-01 00:00:00pages
1096-8issue
3eissn
0099-2240issn
1098-5336journal_volume
62pub_type
杂志文章abstract::Rumen-protected glucose (RPG) plays an important role in alleviating the negative energy balance of dairy cows. This study used a combination of rumen microbes 16S and metabolomics to elucidate the changes of rumen microbial composition and rumen metabolites of different doses of RPG's rumen degradation part in early-...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01908-20
更新日期:2021-01-04 00:00:00
abstract::Campylobacter jejuni was isolated from 18 of 40 processed broiler carcasses and 134 of 327 cloacal swabs obtained at four processing plants in Sydney, Australia. Three of four flocks examined carried C. jejuni. Eighty-two percent of chicken and 98% of human isolates from the area were of identical biotypes. ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.43.5.1219-1220.1982
更新日期:1982-05-01 00:00:00
abstract::A bacterial strain (strain S5) which grows aerobically with the sulfonated azo compound 4-carboxy-4'-sulfoazobenzene as the sole source of carbon and energy was isolated. This strain was obtained by continuous adaptation of "Hydrogenophaga palleronii" S1, which has the ability to grow aerobically with 4-aminobenzenesu...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.64.6.2315-2317.1998
更新日期:1998-06-01 00:00:00
abstract::Aerobic and anaerobic microorganisms were enumerated in tubercles collected from sections of the water distribution pipeline in the Columbus, Ohio, metropolitan area. Coliform bacteria were not detected in the tubercles examined. Sulfate-reducing bacteria were detected in 80% of the samples. Nitrate-reducing heterotro...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.44.3.761-764.1982
更新日期:1982-09-01 00:00:00
abstract::The worldwide decline in honeybee colonies during the past 50 years has often been linked to the spread of the parasitic mite Varroa destructor and its interaction with certain honeybee viruses. Recently in the United States, dramatic honeybee losses (colony collapse disorder) have been reported; however, there remain...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02227-09
更新日期:2009-11-01 00:00:00
abstract::This study used the technique of waiting time modeling to analyze the combined effects of temperature, pH, carbohydrate, protein, and lipid on the time-to-toxicity of Clostridium botulinum 56A. Waiting time models can be used whenever the time to the occurrence of some event is the variable of interest. In the case of...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.64.11.4416-4422.1998
更新日期:1998-11-01 00:00:00
abstract::Nervous necrosis virus (NNV) is a member of the Betanodavirus genus that causes fatal diseases in over 40 species of fish worldwide. Mortality among NNV-infected fish larvae is almost 100%. In order to elucidate the mechanisms responsible for the susceptibility of fish larvae to NNV, we exposed zebrafish larvae to NNV...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.04175-14
更新日期:2015-05-15 00:00:00
abstract::A proteinaceous substance with antibiotic-like activity, resembling that of a bacteriocin, was isolated from an industrial-scale acetone-butanol fermentation of Clostridium acetobutylicum. The substance, purified by acetone precipitation, diethylaminoethyl cellulose chromatography, and polyacrylamide gel electrophores...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.41.2.371-374.1981
更新日期:1981-02-01 00:00:00
abstract::Erwinia amylovora bacteriophages (phages) belonging to the Myoviridae and Podoviridae families demonstrated a preference for either high-exopolysaccharide-producing (HEP) or low-exopolysaccharide-producing (LEP) bacterial hosts when grown on artificial medium without or with sugar supplementation. Myoviridae phages pr...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00067-13
更新日期:2013-05-01 00:00:00
abstract::In Pseudomonas aeruginosa, the catabolite repression control (Crc) protein repressed the formation of the blue pigment pyocyanin in response to a preferred carbon source (succinate) by interacting with phzM mRNA, which encodes a key enzyme in pyocyanin biosynthesis. Crc bound to an extended imperfect recognition seque...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00026-12
更新日期:2012-07-01 00:00:00
abstract::The occurrence and distribution of magnetotactic bacteria (MB) were studied as a function of the physical and chemical conditions in meromictic Salt Pond, Falmouth, Mass., throughout summer 2002. Three dominant MB morphotypes were observed to occur within the chemocline. Small microaerophilic magnetite-producing cocci...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.70.10.6230-6239.2004
更新日期:2004-10-01 00:00:00
abstract::Progesterone was converted to 5alpha-pregnane-3alpha-ol-20-one, delta4-pregnene-20alpha-ol-3-one, delta4-pregnene-14alpha-ol-3,20-dione, delta4-pregnene-7beta,14alpha-diol-3,20-dione, and delta4-pregnene-6beta,11alpha-diol-3,20-dione by cell cultures of Lycopersicon esculentum. Cell cultures of Capsicum frutescens (gr...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.36.2.213-216.1978
更新日期:1978-08-01 00:00:00
abstract::Oil field bacteria were characterized by cloning and sequencing of PCR-amplified 16S rRNA genes. A variety of gram-negative, sulfate-reducing bacteria was detected (16 members of the family Desulfovibrionaceae and 8 members of the family Desulfobacteriaceae). In contrast, a much more limited number of anaerobic, ferme...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.62.5.1623-1629.1996
更新日期:1996-05-01 00:00:00
abstract::A complex network regulates virulence in Ralstonia solanacearum (formerly Pseudomonas solanacearum); central to this system is PhcA, a LysR-type transcriptional regulator. We report here that two PhcA-regulated virulence factors, endoglucanase (Egl) and acidic exopolysaccharide I (EPS I), and motility are expressed di...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.63.3.844-850.1997
更新日期:1997-03-01 00:00:00
abstract::A Rhizobium strain isolated from stem nodules of the legume Sesbania rostrata was shown to grow on atmospheric nitrogen (N(2)) as the sole nitrogen source. Non-N(2)-fixing mutants isolated directly on agar plates formed nodules that did not fix N(2) when inoculated into the host plant. ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.45.2.711-713.1983
更新日期:1983-02-01 00:00:00
abstract::The discovery of a letter intentionally filled with dried Bacillus anthracis spores in the office of a United States senator prompted the collection and quarantine of all mail in congressional buildings. This mail was subsequently searched for additional intentionally contaminated letters. A microbiological sampling s...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00940-06
更新日期:2006-08-01 00:00:00
abstract::To assess the extent of genotypic and phenotypic diversity within species of purple nonsulfur bacteria found in aquatic sediments, a total of 128 strains were directly isolated from agar plates that had been inoculated with sediment samples from Haren and De Biesbosch in The Netherlands. All isolates were initially ch...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.68.7.3467-3477.2002
更新日期:2002-07-01 00:00:00
abstract::A long-chain aldehyde dehydrogenase, Ald1, was found in a soluble fraction of Acinetobacter sp. strain M-1 cells grown on n-hexadecane as a sole carbon source. The gene (ald1) was cloned from the chromosomal DNA of the bacterium. The open reading frame of ald1 was 1,512 bp long, corresponding to a protein of 503 amino...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.66.8.3481-3486.2000
更新日期:2000-08-01 00:00:00
abstract::The restriction endonuclease Cac824I has been shown to be a major barrier to electrotransformation of Clostridium acetobutylicum ATCC 824 (L. D. Mermelstein, N. E. Welker, G. N. Bennett, and E. T. Papoutsakis, Bio/Technology 10:190-195, 1992). Methylation by the phi 3T I methyltransferase encoded by Bacillus subtilis ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.59.4.1077-1081.1993
更新日期:1993-04-01 00:00:00
abstract::L-glutamate overproduction in Corynebacterium glutamicum, a biotin auxotroph, is induced by biotin limitation or by treatment with certain fatty acid ester surfactants or with penicillin. We have analyzed the relationship between the inductions, 2-oxoglutarate dehydrogenase complex (ODHC) activity, and L-glutamate pro...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01867-06
更新日期:2007-02-01 00:00:00
abstract::We investigated associations of species of the Bacteroides fragilis group with Japanese cedar pollinosis (JCPsis). Cell numbers of Bacteroides fragilis and Bacteroides intestinalis were significantly higher in JCPsis subjects than in non-JCPsis subjects before the pollen season. They correlated positively with both sy...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01106-08
更新日期:2008-11-01 00:00:00
abstract::Mycoplasma canadense and Mycoplasma verecundum were cultured in a serum-free medium containing bovine serum albumin, cholesterol, oleic acid, and palmitic acid in order to avoid the addition of horse serum. Growth was detected by measurement of A640 and by colony formation. The level of growth attained in this medium ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.56.7.2259-2261.1990
更新日期:1990-07-01 00:00:00
abstract::This study was conducted to examine the effects of 0.3-Mrad irradiation on growth and toxigenicity of Clostridium botulinum types A and B on chicken skins. Irradiation followed by aerobic or anaerobic incubation at 30 degrees C extended the shelf life of skin samples and delayed growth and toxin production by C. botul...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.53.1.201-203.1987
更新日期:1987-01-01 00:00:00
abstract::A competitive PCR technique was used to enumerate the proteolytic bacterium Clostridium proteoclasticum from the rumen. A PCR primer, which circumscribes this organism and several closely related strains, was designed for a variable region within their 16S rRNA genes and was used in conjunction with a universal forwar...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.64.3.907-913.1998
更新日期:1998-03-01 00:00:00
abstract::The genotyping of numerous isolates of Cryptosporidium parasites has led to the definition of new species and a better understanding of the epidemiology of cryptosporidiosis. A single-locus genotyping method based on the partial sequence of a polymorphic sporozoite surface glycoprotein gene (GP60) has been favored by ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01268-10
更新日期:2010-10-01 00:00:00
abstract::A polyphasic approach involving cultivation, direct viable counts, rRNA-based phylogenetic classification, and in situ probing was applied for the characterization of the dominant microbial population in a municipal drinking water distribution system. A total of 234 bacterial strains cultivated on R2A medium were scre...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.63.11.4164-4170.1997
更新日期:1997-11-01 00:00:00
abstract::Compared to freshwater and the open ocean, less is known about bacterioplankton community structure and spatiotemporal dynamics in estuaries, particularly those with long residence times. The Chesapeake Bay is the largest estuary in the United States, but despite its ecological and economic significance, little is kno...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00541-07
更新日期:2007-11-01 00:00:00
abstract::A compact housing unit for low-power (X6 to X50) cinemicrographic studies of microbial specimens in the vertical plane is described. This unit was used successfully to record the development of a "halo" of cells around subsurface colonies of Escherichia coli. ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.32.2.294-297.1976
更新日期:1976-08-01 00:00:00
abstract::We developed a PCR detection method that selectively recognizes a single biological control agent and demonstrated that universally primed PCR (UP-PCR) can identify strain-specific markers. Antagonistic strains of Clonostachys rosea (syn. Gliocladium roseum) were screened by UP-PCR, and a strain-specific marker was id...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.66.11.4758-4763.2000
更新日期:2000-11-01 00:00:00
abstract::The presence and activities of urease genes were investigated in 49 clinical, food, and environmental Bacillus cereus isolates. Ten strains were shown to have urease genes, with eight of these strains showing growth on urea as the sole nitrogen source. Two of the urease-positive strains, including the sequenced strain...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02737-07
更新日期:2008-04-01 00:00:00