Characterization of urease-positive thermophilic Campylobacter subspecies by multilocus enzyme electrophoresis typing.

abstract：:Microscale fluorescence measurements were performed in photosynthetic biofilms at a spatial resolution of 100 to 200 microm with a new fiber-optic fluorometer which allowed four different excitation and emission wavelengths and was configured for measuring phycobiliproteins, chlorophylls, and bacteriochlorophylls. We ...

journal_title：Applied and environmental microbiology

authors： Thar R,Kühl M,Holst G

更新日期：2001-06-01 00:00:00

abstract：:Proteolytic but chitinase-deficient microbial cultures were isolated from shrimp shell waste and characterized. The most efficient isolate was found to be a mixed culture consisting of two Bacillus licheniformis strains, which were first determined microscopically and physiologically. Molecular characterization was ca...

journal_title：Applied and environmental microbiology

authors： Waldeck J,Daum G,Bisping B,Meinhardt F

更新日期：2006-12-01 00:00:00

abstract：UNLABELLED:Stress-induced abdominal dysfunction is an attractive target for probiotics. To investigate the effects of the probiotic Lactobacillus casei strain Shirota on abdominal dysfunction, a double-blind, placebo-controlled trial was conducted with healthy medical students undertaking an authorized nationwide exami...

journal_title：Applied and environmental microbiology

authors： Kato-Kataoka A,Nishida K,Takada M,Kawai M,Kikuchi-Hayakawa H,Suda K,Ishikawa H,Gondo Y,Shimizu K,Matsuki T,Kushiro A,Hoshi R,Watanabe O,Igarashi T,Miyazaki K,Kuwano Y,Rokutan K

更新日期：2016-05-31 00:00:00

abstract：:Deep-subsurface samples obtained by deep drilling are likely to be contaminated with mesophilic microorganisms in the drilling fluid, and this could affect determination of the community structure of the geothermal microflora using 16S rRNA gene clone library analysis. To eliminate possible contamination by PCR-amplif...

journal_title：Applied and environmental microbiology

authors： Kimura H,Sugihara M,Kato K,Hanada S

更新日期：2006-01-01 00:00:00

abstract：:Two methods were used to evaluate bacterial recovery from beef, pork, and lamb adipose tissue. Higher counts were obtained with a tissue removal and fluid agitation technique (shaking) than with surface swabbing, but only when bacterial levels were low. Bacterial recovery by both methods was unaffected by specie origi...

journal_title：Applied and environmental microbiology

authors： Berry BW,Joseph AL,Chen AA

更新日期：1978-05-01 00:00:00

abstract：:We previously demonstrated that xanthobaccin A from the rhizoplane bacterium Lysobacter sp. strain SB-K88 suppresses damping-off disease caused by Pythium sp. in sugar beet. In this study we focused on modes of Lysobacter sp. strain SB-K88 root colonization and antibiosis of the bacterium against Aphanomyces cochlioid...

journal_title：Applied and environmental microbiology

authors： Islam MT,Hashidoko Y,Deora A,Ito T,Tahara S

更新日期：2005-07-01 00:00:00

abstract：:The luxS gene of Lactobacillus reuteri 100-23C was amplified by PCR, cloned, and then sequenced. To define a physiological and ecological role for the luxS gene in L. reuteri 100-23C, a luxS mutant was constructed by insertional mutagenesis. The luxS mutant did not produce autoinducers AI-2 or AI-3. Complementation of...

journal_title：Applied and environmental microbiology

authors： Tannock GW,Ghazally S,Walter J,Loach D,Brooks H,Cook G,Surette M,Simmers C,Bremer P,Dal Bello F,Hertel C

更新日期：2005-12-01 00:00:00

abstract：:A number of Micromonospora strains isolated from the water column, sediment, and cellulose baits placed in freshwater lakes were shown to be able to degrade cellulose in lake water without any addition of nutrients. A selective isolation method was also developed to demonstrate that CFU arose from both spores and hyph...

journal_title：Applied and environmental microbiology

authors： de Menezes AB,Lockhart RJ,Cox MJ,Allison HE,McCarthy AJ

更新日期：2008-11-01 00:00:00

abstract：:Although extraradicular biofilm formation is related to refractory periapical periodontitis, the mechanism of extraradicular biofilm development, as well as its effect on periapical lesions, is unknown. Therefore, we aimed to develop an in vivo extraradicular biofilm model in rats and to identify and quantify extrarad...

journal_title：Applied and environmental microbiology

authors： Kuremoto K,Noiri Y,Ishimoto T,Yoneda N,Yamamoto R,Maezono H,Nakano T,Hayashi M,Ebisu S

更新日期：2014-07-01 00:00:00

abstract：:Fourier transform infrared (FTIR) spectroscopy in combination with the partial least squares (PLS) multivariative statistical technique was used for quantitative analysis of the poly(beta-hydroxybutyrate) (PHB) contents of bacterial cells. A total of 237 replicate spectra from 34 samples were obtained together with ga...

journal_title：Applied and environmental microbiology

authors： Kansiz M,Billman-Jacobe H,McNaughton D

更新日期：2000-08-01 00:00:00

abstract：:Carbon-starved cultures of strain Ant-300, a psychrophilic marine vibrio isolated from the Antarctic Convergence, were compared with their nonstarved counterparts for resistance to heat. Specifically, starved and unstarved cells were exposed to 17 degrees C, which is 4 degrees C above the maximum growth temperature, a...

journal_title：Applied and environmental microbiology

authors： Preyer JM,Oliver JD

更新日期：1993-08-01 00:00:00

abstract：:The Pseudomonas putida KT2440 TOL upper pathway is repressed under nonlimiting conditions in cells growing in chemostat with succinate as a carbon source. We show that the ptsN gene product IIA(Ntr) participates in this repression. Crc, involved in yeast extract-dependent repression in batch cultures, did not influenc...

journal_title：Applied and environmental microbiology

authors： Aranda-Olmedo I,Marín P,Ramos JL,Marqués S

更新日期：2006-11-01 00:00:00

abstract：:Pyrene is a regulated pollutant at sites contaminated with polycyclic aromatic hydrocarbons (PAH). It is mineralized by some bacteria but is also transformed to nonmineral products by a variety of other PAH-degrading bacteria. We examined the formation of such products by four bacterial strains and identified and furt...

journal_title：Applied and environmental microbiology

authors： Kazunga C,Aitken MD

更新日期：2000-05-01 00:00:00

abstract：:In Corynebacterium glutamicum, the ArgR protein, a transcriptional repressor, affects the expression level of the argB gene through binding to its promoter region. The argB promoter region (positions -77 to -25) has been found by in vitro electrophoretic mobility shift assay (EMSA) results and in silico analysis to be...

journal_title：Applied and environmental microbiology

authors： Lee SY,Park JM,Lee JH,Chang ST,Park JS,Kim YH,Min J

更新日期：2011-02-01 00:00:00

abstract：:Heterologous protein production can be doubled by increasing the copy number of the corresponding heterologous gene. We constructed a host-vector system in the yeast Kluyveromyces lactis that was able to induce copy number amplification of pKD1 plasmid-based vectors upon expression of an integrated copy of the plasmid...

journal_title：Applied and environmental microbiology

authors： Morlino GB,Tizzani L,Fleer R,Frontali L,Bianchi MM

更新日期：1999-11-01 00:00:00

abstract：:Low levels of tetracyclines found as residues in milk inhibited the biosynthesis of beta-galactosidase in Escherichia coli. To produce the same effect, other antibacterials had to occur in concentrations that were more than 10-fold higher. This relative selectivity was exploited for the development of a screening test...

journal_title：Applied and environmental microbiology

authors： D'Haese E,Nelis HJ,Reybroeck W

更新日期：1997-10-01 00:00:00

abstract：:Members of the genus Sphingomonas are important catalysts for removal of polycyclic aromatic hydrocarbons (PAHs) in soil, but their activity can be affected by various stress factors. This study examines the physiological and genome-wide transcription response of the phenanthrene-degrading Sphingomonas sp. strain LH12...

journal_title：Applied and environmental microbiology

authors： Fida TT,Breugelmans P,Lavigne R,Coronado E,Johnson DR,van der Meer JR,Mayer AP,Heipieper HJ,Hofkens J,Springael D

更新日期：2012-12-01 00:00:00

abstract：:Sterol demethylation inhibitor (DMI) fungicides are widely used to control fungi pathogenic to humans and plants. Resistance to DMIs is mediated either through alterations in the structure of the target enzyme CYP51 (encoding 14alpha-demethylase), through increased expression of the CYP51 gene, or through increased ex...

journal_title：Applied and environmental microbiology

authors： Ma Z,Proffer TJ,Jacobs JL,Sundin GW

更新日期：2006-04-01 00:00:00

abstract：:A European baseline survey during the years 2005 and 2006 has revealed that the monophasic Salmonella enterica subsp. enterica serovar 4,12:d:- was, with a prevalence of 23.6%, the most frequently isolated serovar in German broiler flocks. In Denmark and the United Kingdom, its serovar prevalences were 15.15% and 2.8%...

journal_title：Applied and environmental microbiology

authors： Huehn S,Bunge C,Junker E,Helmuth R,Malorny B

更新日期：2009-02-01 00:00:00

abstract：:Thiolase (acetyl coenzyme A acetyltransferase; EC 2.3.1.9) from Clostridium acetobutylicum is a key enzyme in the production of acids and solvents in this organism. The purification and properties of the enzyme have already been described (D. P. Wiesenborn, F. B. Rudolph, and E.T. Papoutsakis, Appl. Environ. Microbiol...

journal_title：Applied and environmental microbiology

authors： Petersen DJ,Bennett GN

更新日期：1991-09-01 00:00:00

abstract：:The UV sunscreen role commonly ascribed to mycosporine-like amino acids (MAAs) was investigated with an isolate of the terrestrial cyanobacterium Gloeocapsa sp. strain C-90-Cal-G.(2), which accumulates intracellularly an MAA with absorbance maximum at 326 nm but produces no extracellular sunscreen compound (i.e., scyt...

journal_title：Applied and environmental microbiology

authors： Garcia-Pichel F,Wingard CE,Castenholz RW

更新日期：1993-01-01 00:00:00

abstract：:The effect of addition of purified nisin Z in liposomes to cheese milk and of in situ production of nisin Z by Lactococcus lactis subsp. lactis biovar diacetylactis UL719 in the mixed starter on the inhibition of Listeria innocua in cheddar cheese was evaluated during 6 months of ripening. A cheese mixed starter cultu...

journal_title：Applied and environmental microbiology

authors： Benech RO,Kheadr EE,Laridi R,Lacroix C,Fliss I

更新日期：2002-08-01 00:00:00

abstract：:A novel multiplex PCR method using three sets of specific primers was developed for the detection of the cytotoxic (act), heat-labile (alt), and heat-stable (ast) enterotoxin genes in Aeromonas spp. This assay was used to characterize 35 reference strains as well as 537 food-borne isolates. A total of seven gene patte...

journal_title：Applied and environmental microbiology

authors： Kingombe CI,D'Aoust JY,Huys G,Hofmann L,Rao M,Kwan J

更新日期：2010-01-01 00:00:00

abstract：:To ascertain the health risks that may be posed by the land application of sewage sludges, a scheme was devised to determine the types and numbers of pathogenic and potentially pathogenic bacteria present in sludges. A processing treatment was adapted to sludge to give a homogenate which yielded the greatest numbers o...

journal_title：Applied and environmental microbiology

authors： Dudley DJ,Guentzel MN,Ibarra MJ,Moore BE,Sagik BP

更新日期：1980-01-01 00:00:00

abstract：:Polyhydroxyalkanoates (PHAs) are microbial polyesters that can be used as completely biodegradable polymers, but the high production cost prevents their use in a wide range of applications. Recombinant Escherichia coli strains harboring the Ralstonia eutropha PHA biosynthesis genes have been reported to have several a...

journal_title：Applied and environmental microbiology

authors： Choi JI,Lee SY,Han K

更新日期：1998-12-01 00:00:00

abstract：:Preliminary observations in a large-scale fermentation process suggested that the lipase expression of Pseudomonas alcaligenes can be switched on by the addition of certain medium components, such as soybean oil. In an attempt to elucidate the mechanism of induction of lipase expression, we have set up a search method...

journal_title：Applied and environmental microbiology

authors： Krzeslak J,Gerritse G,van Merkerk R,Cool RH,Quax WJ

更新日期：2008-03-01 00:00:00

abstract：:Five species of the genus Dunaliella (D. tertiolecta, D. primolecta, D. parva, D. bardawil, and D. salina) were examined for glycerol accumulation, growth rate, cell density, and protein and chlorophyll content. The suitability of each algal species for use as a fermentation substrate was judged according to glycerol ...

journal_title：Applied and environmental microbiology

authors： Nakas JP,Schaedle M,Parkinson CM,Coonley CE,Tanenbaum SW

更新日期：1983-11-01 00:00:00

abstract：:This report describes the use of an oligonucleotide macroarray to profile the expression of 375 genes in Lactococcus lactis subsp. lactis IL1403 during heat, acid, and osmotic stress. A set of known stress-associated genes in IL1403 was used as the internal control on the array. Every stress response was accurately de...

journal_title：Applied and environmental microbiology

authors： Xie Y,Chou LS,Cutler A,Weimer B

更新日期：2004-11-01 00:00:00

abstract：:Giardia muris cysts were separated from fecal contaminants in primary isolates by unit gravity velocity sedimentation. Crude isolates obtained by centrifugation over 1.0 M sucrose were overlaid onto a Percoll density gradient, 1.01 to 1.03 g/ml. G. muris cysts were well separated from faster-sedimenting fecal debris a...

journal_title：Applied and environmental microbiology

authors： Sauch JF

更新日期：1984-08-01 00:00:00

abstract：:The diversity of sulfate-reducing bacteria (SRB) in brackish sediment was investigated using small-subunit rRNA and dissimilatory sulfite reductase (DSR) gene clone libraries and cultivation. The phylogenetic affiliation of the most commonly retrieved clones for both genes was strikingly similar and produced Desulfosa...

journal_title：Applied and environmental microbiology

authors： Joulian C,Ramsing NB,Ingvorsen K

更新日期：2001-07-01 00:00:00