System development for linked-fermentation production of solvents from algal biomass.

Abstract:

:Five species of the genus Dunaliella (D. tertiolecta, D. primolecta, D. parva, D. bardawil, and D. salina) were examined for glycerol accumulation, growth rate, cell density, and protein and chlorophyll content. The suitability of each algal species for use as a fermentation substrate was judged according to glycerol accumulation and quantities of neutral solvents produced after sequential bacterial fermentations. When grown in 2 M NaCl, with 24 mM NaHCO(3) or 3% CO(2) at 28 degrees C and with 10,000 to 15,000 lx of incident light on two sides of a glass aquarium, four of the five species tested produced ca. 10 to 20 mg of glycerol per liter of culture. Clostridium pasteurianum was found to convert an algal biomass mixture supplemented with 4% glycerol to ca. 16 g of mixed solvents (n-butanol, 1,3-propanediol, and ethanol) per liter. Acetone was not detected. Additionally, it has been demonstrated that Dunaliella concentrates of up to 300-fold can be directly fermented to an identical pattern of mixed solvents. Overall solvent yields were reduced by >50% when fermentations were performed in the presence of 2% NaCl. These results are discussed in terms of practical application in tropical coastal zones.

journal_name

Appl Environ Microbiol

authors

Nakas JP,Schaedle M,Parkinson CM,Coonley CE,Tanenbaum SW

doi

10.1128/AEM.46.5.1017-1023.1983

keywords:

subject

Has Abstract

pub_date

1983-11-01 00:00:00

pages

1017-23

issue

5

eissn

0099-2240

issn

1098-5336

journal_volume

46

pub_type

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