Prevalence and abundance of uncultivated Megasphaera-like bacteria in the human vaginal environment.

abstract：:We detected and identified genotypes of human-pathogenic microsporidia in fecal samples from 51 asymptomatic captive-bred pet parrots in South Korea. Microsporidia were identified in 8 samples (15.7%); 7 parrots tested positive for Encephalitozoon hellem, and 1 parrot tested positive for both E. hellem and Encephalito...

journal_title：Applied and environmental microbiology

authors： Lee SY,Lee SS,Lyoo YS,Park HM

更新日期：2011-12-01 00:00:00

abstract：:Legionella pneumophila, the agent of Legionnaires' disease, is an intracellular parasite of aquatic amoebae and human macrophages. A key factor for L. pneumophila in intracellular infection is its type II protein secretion system (Lsp). In order to more completely define Lsp output, we recently performed a proteomic a...

journal_title：Applied and environmental microbiology

authors： Rossier O,Dao J,Cianciotto NP

更新日期：2008-02-01 00:00:00

abstract：:We developed a PCR detection method that selectively recognizes a single biological control agent and demonstrated that universally primed PCR (UP-PCR) can identify strain-specific markers. Antagonistic strains of Clonostachys rosea (syn. Gliocladium roseum) were screened by UP-PCR, and a strain-specific marker was id...

journal_title：Applied and environmental microbiology

authors： Bulat SA,Lübeck M,Alekhina IA,Jensen DF,Knudsen IM,Lübeck PS

更新日期：2000-11-01 00:00:00

abstract：:Current models suggest that (i) filamentous bacteria are protected against predation by nanoflagellates, (ii) prey size is positively correlated with prey-predator contact probability, and (iii) contact probability is mainly responsible for size-selective predation by interception-feeding flagellates. We used five str...

journal_title：Applied and environmental microbiology

authors： Wu QL,Boenigk J,Hahn MW

更新日期：2004-01-01 00:00:00

abstract：:Lyophilization was used to concentrate bovine feces prior to DNA extraction and analysis using real-time PCR. Lyophilization significantly improved the sensitivity of detection compared to that in fresh feces and was associated with reliable quantification of both Escherichia coli O157:H7 and Campylobacter jejuni bact...

journal_title：Applied and environmental microbiology

authors： Rapp D,Waller J,Brightwell G,Muirhead RW

更新日期：2010-03-01 00:00:00

abstract：:Helix alpha 4 of Bacillus thuringiensis Cry toxins is thought to play a critical role in the toxins' mode of action. Accordingly, single-site substitutions of many Cry1Aa helix alpha 4 amino acid residues have previously been shown to cause substantial reductions in the protein's pore-forming activity. Changes in prot...

journal_title：Applied and environmental microbiology

authors： Girard F,Vachon V,Préfontaine G,Marceau L,Schwartz JL,Masson L,Laprade R

更新日期：2009-01-01 00:00:00

abstract：:The production of a stable cDNA copy of an unstable RNA molecule by reverse transcription is a widely used and essential technology for many important applications, such as the construction of gene libraries, production of DNA probes, and analysis of gene expression by reverse transcriptase PCR (RT-PCR). However, the ...

journal_title：Applied and environmental microbiology

authors： Vellore J,Moretz SE,Lampson BC

更新日期：2004-12-01 00:00:00

abstract：:The plasmid pYDH208, which confers the ability to catabolize the mannityl opines mannopine and agropine, was mobilized into the nonpathogenic Pseudomonas syringae strain Cit7. The growth of the mannityl opine-catabolizing strain Cit7(pYDH208) was compared with that of the near-isogenic non-opine-catabolizing strain Ci...

journal_title：Applied and environmental microbiology

authors： Wilson M,Savka MA,Hwang I,Farrand SK,Lindow SE

更新日期：1995-06-01 00:00:00

abstract：:Hepatitis A virus disinfection was assessed on contaminated stainless-steel disks. Ten microliters of fecally suspended hepatitis A virus was deposited on the center of each disk, dried for 20 min, and then covered with 20 microliters of the test product for 1 min. Of the 20 formulations tested, only 2% glutaraldehyde...

journal_title：Applied and environmental microbiology

authors： Mbithi JN,Springthorpe VS,Sattar SA

更新日期：1990-11-01 00:00:00

abstract：:The effects of various nitrogen sources and of the cultivation time on the biosynthesis of N-labelled azoverdin were studied in batch cultures of Azomonas macrocytogenes ATCC 12334. Growth for 48 h in the presence of ammonium sulfate (0.2 g/liter) resulted in 95% labelling of azoverdin with N. A growth-related synthes...

journal_title：Applied and environmental microbiology

authors： Dobisová M,Abdallah MA,Kyslík P

更新日期：1994-12-01 00:00:00

abstract：:The doses of UV irradiation necessary to inactivate selected enteric viruses on the U.S. Environmental Protection Agency Contaminant Candidate List were determined. Three-log reductions of echovirus 1, echovirus 11, coxsackievirus B3, coxsackievirus B5, poliovirus 1, and human adenovirus type 2 were effected by doses ...

journal_title：Applied and environmental microbiology

authors： Gerba CP,Gramos DM,Nwachuku N

更新日期：2002-10-01 00:00:00

abstract：:Rhodococcus equi is a leading cause of severe pneumonia in foals. Standard treatment is dual antimicrobial therapy with a macrolide and rifampin, but the emergence of macrolide- and rifampin-resistant R. equi isolates is an increasing problem. The objective of this study was to determine the effect of macrolide and/or...

journal_title：Applied and environmental microbiology

authors： Willingham-Lane JM,Berghaus LJ,Berghaus RD,Hart KA,Giguère S

更新日期：2019-03-22 00:00:00

abstract：:For simultaneous identification of members of the betaproteobacterial order "Rhodocyclales" in environmental samples, a 16S rRNA gene-targeted oligonucleotide microarray (RHC-PhyloChip) consisting of 79 probes was developed. Probe design was based on phylogenetic analysis of available 16S rRNA sequences from all cultu...

journal_title：Applied and environmental microbiology

authors： Loy A,Schulz C,Lücker S,Schöpfer-Wendels A,Stoecker K,Baranyi C,Lehner A,Wagner M

更新日期：2005-03-01 00:00:00

abstract：:Noroviruses, which are members of the Caliciviridae family, represent the leading cause of nonbacterial gastroenteritis in developed countries; such norovirus infections result in high economic costs for health protection. Person-to-person contact, contaminated water, and foods, especially raw shellfish, vegetables, a...

journal_title：Applied and environmental microbiology

authors： Buckow R,Isbarn S,Knorr D,Heinz V,Lehmacher A

更新日期：2008-02-01 00:00:00

abstract：:Twenty-six subsurface samples were collected from a borehole at depths of 173.3 to 196.8 m in the saturated zone at the Hanford Site in south-central Washington State. The sampling was performed throughout strata that included fine-grained lacustrine (lake) sediments, a paleosol (buried soil) sequence, and coarse-grai...

journal_title：Applied and environmental microbiology

authors： Kieft TL,Fredrickson JK,McKinley JP,Bjornstad BN,Rawson SA,Phelps TJ,Brockman FJ,Pfiffner SM

更新日期：1995-02-01 00:00:00

abstract：:Melanin is a pigment produced by organisms throughout all domains of life. Due to its unique physicochemical properties, biocompatibility, and biostability, there has been an increasing interest in the use of melanin for broad applications. In the vast majority of studies, melanin has been either chemically synthesize...

journal_title：Applied and environmental microbiology

authors： Wang Z,Tschirhart T,Schultzhaus Z,Kelly EE,Chen A,Oh E,Nag O,Glaser ER,Kim E,Lloyd PF,Charles PT,Li W,Leary D,Compton J,Phillips DA,Dhinojwala A,Payne GF,Vora GJ

更新日期：2020-02-18 00:00:00

abstract：:We previously reported the isolation of a 21.5-kb genomic DNA fragment from Pseudomonas sp. strain ADP, which contains the atzA gene, encoding the first metabolic step for the degradation of the herbicide atrazine (M. de Souza, L. P. Wackett, K. L. Boundy-Mills, R. T. Mandelbaum, and M. J. Sadowsky, Appl. Environ. Mic...

journal_title：Applied and environmental microbiology

authors： Boundy-Mills KL,de Souza ML,Mandelbaum RT,Wackett LP,Sadowsky MJ

更新日期：1997-03-01 00:00:00

abstract：:A novel Bacillus thuringiensis crystal protein with a silent activity against the Colorado potato beetle is described. The crystal proteins are produced as bipyramidal crystals. These crystals contain a protein of 129 kDa with a trypsin-resistant core fragment of 72 kDa. Neither a spore-crystal mixture nor in vitro-so...

journal_title：Applied and environmental microbiology

authors： Lambert B,Höfte H,Annys K,Jansens S,Soetaert P,Peferoen M

更新日期：1992-08-01 00:00:00

abstract：:After treatment of a dispersed suspension of echovirus with HOCl, much of the lost plaque titer was restored if the treated virus was induced to aggregate by adjustment of the suspending medium to pH 4.5. This did not appear to be a repair of individual virions but rather a special kind of multiplicity-related increas...

journal_title：Applied and environmental microbiology

authors： Young DC,Sharp DG

更新日期：1979-04-01 00:00:00

abstract：:A new design, flat-bottom combination pH electrode was evaluated for utility in determining the pH of prepared agar media by surface contact of the electrode with the agar media. ...

journal_title：Applied and environmental microbiology

authors： Gorski TW,Ritzert RW

更新日期：1977-08-01 00:00:00

abstract：:The temperate bacteriophage phiLC3, isolated from Lactococcus lactis subsp. cremoris, has an isometric head and a flexible tail containing 1 major protein and 8 minor proteins. Infection of a permissive L. lactis host strain yields a burst of about 50 phages per infected cell with a latent period of 60 min. A detailed...

journal_title：Applied and environmental microbiology

authors： Lillehaug D,Lindqvist B,Birkeland NK

更新日期：1991-11-01 00:00:00

abstract：:Iron-oxidizing thiobacilli were adapted to grow on a chalcopyrite and a galena ore concentrate. When grown on the chalcopyrite concentrate, the bacteria exhibited a doubling time of 38.4 +/- 2.9 h, with a final cellular protein concentration of 185 mug/ml and solubilization of 10.3 g of copper per liter. When grown on...

journal_title：Applied and environmental microbiology

authors： Kingma JG,Silver M

更新日期：1980-03-01 00:00:00

abstract：:A mutant of the peptide antibiotic nisin in which the dehydroalanine residue at position 5 has been replaced by an alanine has been produced and structurally characterized. It is shown to have activity very similar to that of wild-type nisin in inhibiting growth of Lactococcus lactis and Micrococcus luteus but is very...

journal_title：Applied and environmental microbiology

authors： Chan WC,Dodd HM,Horn N,Maclean K,Lian LY,Bycroft BW,Gasson MJ,Roberts GC

更新日期：1996-08-01 00:00:00

abstract：:Information on the interaction between mixed populations in the rumen and plant phenolics is required to fully elucidate the limitations of phenolic compounds on forage digestibility. The objective of this study was to examine the degradation of Italian ryegrass (Lolium multiflorum L.) hay incubated with mixed ruminal...

journal_title：Applied and environmental microbiology

authors： Theodorou MK,Gascoyne DJ,Akin DE,Hartley RD

更新日期：1987-05-01 00:00:00

abstract：:Based on elementary mode analysis, an Escherichia coli strain was designed for efficient conversion of glycerol to ethanol. By using nine gene knockout mutations, the functional space of the central metabolism of E. coli was reduced from over 15,000 possible pathways to a total of 28 glycerol-utilizing pathways that s...

journal_title：Applied and environmental microbiology

authors： Trinh CT,Srienc F

更新日期：2009-11-01 00:00:00

abstract：:In Vibrio vulnificus, virulence for eels is associated with serovar E strains. In this study, we investigated some biological properties of purified lipopolysaccharides (LPSs) from serovar E and non-serovar E strains. Purified LPSs retained their O-polysaccharidic side chains and did not show any differences that coul...

journal_title：Applied and environmental microbiology

authors： Biosca EG,Collado RM,Oliver JD,Amaro C

更新日期：1999-02-01 00:00:00

abstract：:Ten laboratories participated in an interlaboratory comparison of determination of bioleaching rates of a pyrite reference material. A standardized procedure and a single strain of Thiobacillus ferrooxidans were used in this study. The mean rate of bioleaching of the pyrite reference material was 12.4 mg of Fe per lit...

journal_title：Applied and environmental microbiology

authors： Olson GJ

更新日期：1991-03-01 00:00:00

abstract：:Circular bacteriocins are a group of N-to-C-terminally linked antimicrobial peptides, produced by Gram-positive bacteria of the phylum Firmicutes. Circular bacteriocins generally exhibit broad-spectrum antimicrobial activity, including against common food-borne pathogens, such as Clostridium and Listeria spp. These pe...

journal_title：Applied and environmental microbiology

authors： Gabrielsen C,Brede DA,Nes IF,Diep DB

更新日期：2014-11-01 00:00:00

abstract：:The effects of drying time during freeze-drying on the outermost cell surface of an encapsulated strain of Staphylococcus aureus S-7 (Smith, diffuse) were investigated, with special attention paid to capsule and slime production. To quantify capsule and slime production, capsule antigen production and cellular charact...

journal_title：Applied and environmental microbiology

authors： Ohtomo T,Yamada T,Yoshida K

更新日期：1988-10-01 00:00:00

abstract：:Campylobacter jejuni and Campylobacter coli are naturally competent, but limited information exists on the impact of environmental conditions on transformation. In this study, we investigated the impact of temperature and microaerobic versus aerobic atmosphere on transformation of C. coli to erythromycin and nalidixic...

journal_title：Applied and environmental microbiology

authors： Kim JS,Kim JW,Kathariou S

更新日期：2008-10-01 00:00:00