Heterologous expression, purification, and characterization of a highly active xylose reductase from Neurospora crassa.

abstract：:Biofilm bacteria challenged with monochloramine retained significant respiratory activity, even though they could not be cultured on agar plates. Microbial colony counts on agar media declined by approximately 99.9% after 1 h of disinfection, whereas the number of bacteria stained by a fluorescent redox dye experience...

journal_title：Applied and environmental microbiology

authors： Stewart PS,Griebe T,Srinivasan R,Chen CI,Yu FP,deBeer D,McFeters GA

更新日期：1994-05-01 00:00:00

abstract：:Acidophilic archaea of the archaeal Richmond Mine acidophilic nanoorganisms (ARMAN) group from the uncultured candidate phylum "Candidatus Micrarchaeota" have small genomes and cell sizes and are known to be metabolically dependent and physically associated with their Thermoplasmatales hosts. However, phylogenetically...

journal_title：Applied and environmental microbiology

authors： Kadnikov VV,Savvichev AS,Mardanov AV,Beletsky AV,Chupakov AV,Kokryatskaya NM,Pimenov NV,Ravin NV

更新日期：2020-11-10 00:00:00

abstract：:Tellurium, a metalloid belonging to group 16 of the periodic table, displays very interesting physical and chemical properties and lately has attracted significant attention for its use in nanotechnology. In this context, the use of microorganisms for synthesizing nanostructures emerges as an eco-friendly and exciting...

journal_title：Applied and environmental microbiology

authors： Pugin B,Cornejo FA,Muñoz-Díaz P,Muñoz-Villagrán CM,Vargas-Pérez JI,Arenas FA,Vásquez CC

更新日期：2014-11-01 00:00:00

abstract：:NisI confers immunity against nisin, with high substrate specificity to prevent a suicidal effect in nisin-producing Lactococcus lactis strains. However, the NisI maturation process as well as its influence on nisin resistance has not been characterized. Here, we report the roles of lipoprotein signal peptidase II (Ls...

journal_title：Applied and environmental microbiology

authors： Liu J,Xiong H,Du Y,Wiwatanaratanabutr I,Wu X,Zhao G,Zhu H,Caiyin Q,Qiao J

更新日期：2020-09-17 00:00:00

abstract：:[This corrects the article on p. 662 in vol. 45.]. ...

journal_title：Applied and environmental microbiology

authors：

更新日期：1983-06-01 00:00:00

abstract：:Cytolethal distending toxins (CDT) are potent cytotoxins of several Gram-negative pathogenic bacteria, including Escherichia coli, in which five types (CDT-I to CDT-V) have been identified so far. CDT-V is frequently associated with Shiga-toxigenic E. coli (STEC), enterohemorrhagic E. coli (EHEC) O157 strains, and str...

journal_title：Applied and environmental microbiology

authors： Sváb D,Horváth B,Maróti G,Dobrindt U,Tóth I

更新日期：2013-08-01 00:00:00

abstract：:Dextransucrase production by Leuconostoc mesenteroides NRRL B-512F in media containing carbon sources other than sucrose is reported for the first time. Dextransucrases were analyzed by gel electrophoresis and by an in situ activity assay. Their polymers and acceptor reaction products were also compared by (13)C nucle...

journal_title：Applied and environmental microbiology

authors： Quirasco M,López-Munguía A,Remaud-Simeon M,Monsan P,Farrés A

更新日期：1999-12-01 00:00:00

abstract：:Biotin-labeled DNA probes prepared from whole plasmids (5.5 and 4.8 kilobases) of two lactobacillus strains (Lactobacillus delbrueckii 21 and Lactobacillus reuteri 100-23) were used to detect the homologous bacteria in microtome-cryostat-prepared sections of murine forestomach. The forestomach sections were incubated ...

journal_title：Applied and environmental microbiology

authors： Tannock GW

更新日期：1989-02-01 00:00:00

abstract：:The key enzymes and pathways involved in polyhydroxyalkanoate (PHA) biosynthesis in haloarchaea have been identified in recent years, but the haloarchaeal enzymes for PHA degradation remain unknown. In this study, a patatin-like PHA depolymerase, PhaZh1, was determined to be located on the PHA granules in the haloarch...

journal_title：Applied and environmental microbiology

authors： Liu G,Hou J,Cai S,Zhao D,Cai L,Han J,Zhou J,Xiang H

更新日期：2015-05-01 00:00:00

abstract：:Suspensions of Nitrosomonas europaea catalyzed the oxidation of the commercial nitrification inhibitor nitrapyrin [2-chloro-6-(trichloromethyl)-pyridine]. Rapid oxidation of nitrapyrin (at a concentration of 10 muM) required the concomitant oxidation of ammonia, hydroxylamine, or hydrazine. The turnover rate was highe...

journal_title：Applied and environmental microbiology

authors： Vannelli T,Hooper AB

更新日期：1992-07-01 00:00:00

abstract：:The promoter region of the glyceraldehyde-3-phosphate dehydrogenase gene (gpd) was used to drive expression of mnp1, the gene encoding Mn peroxidase isozyme 1, in primary metabolic cultures of Phanerochaete chrysosporium. A 1,100-bp fragment of the P. chrysosporium gpd promoter region was fused upstream of the mnp1 ge...

journal_title：Applied and environmental microbiology

authors： Mayfield MB,Kishi K,Alic M,Gold MH

更新日期：1994-12-01 00:00:00

abstract：:A total of 1,794 migrating birds trapped at a coastal site in southern Sweden were sampled for detection of Campylobacter spp. All isolates phenotypically identified as Campylobacter jejuni and a subset of those identified as non-C. jejuni were identified to the species level by PCR-based techniques. C. jejuni was fou...

journal_title：Applied and environmental microbiology

authors： Waldenström J,Broman T,Carlsson I,Hasselquist D,Achterberg RP,Wagenaar JA,Olsen B

更新日期：2002-12-01 00:00:00

abstract：:Flavobacterium psychrophilum, a member of the Cytophaga-Flavobacterium-Bacteroides group, is an important pathogen of salmonid fish. Previous attempts to develop genetic techniques for this fastidious, psychrotrophic bacterium have met with failure. Here we describe the development of techniques for the genetic manipu...

journal_title：Applied and environmental microbiology

authors： Alvarez B,Secades P,McBride MJ,Guijarro JA

更新日期：2004-01-01 00:00:00

abstract：:Small-subunit ribosomal DNA (SSU rDNA) from 20 phenotypically distinct strains of 2,4-dichlorophenoxyacetic acid (2,4-D)-degrading bacteria was partially sequenced, yielding 18 unique strains belonging to members of the alpha, beta, and gamma subgroups of the class Proteobacteria. To understand the origin of 2,4-D deg...

journal_title：Applied and environmental microbiology

authors： McGowan C,Fulthorpe R,Wright A,Tiedje JM

更新日期：1998-10-01 00:00:00

abstract：:Survivor curves for heat-resistant ascospores of Byssochlamys fulva exposed to lethal heat were nonlogarithmic. At lower heating temperatures, the log survivor curves were characterized by a shoulder plus an accelerating death rate; with increased temperatures, the rate approached logarithmic death. The formula (log N...

journal_title：Applied and environmental microbiology

authors： King AD Jr,Bayne HG,Alderton G

更新日期：1979-03-01 00:00:00

abstract：:Chemolithoautotrophic bacteria were enriched from mine water at incubation temperatures ranging from 4 to 46 degrees C, using elemental sulfur as a substrate in acid mineral salts media. Thiobacillus-type bacteria were successfully enriched for at all test temperatures except 46 degrees C. Changes in pH (-dpH/dt) were...

journal_title：Applied and environmental microbiology

authors： Ahonen L,Tuovinen OH

更新日期：1990-02-01 00:00:00

abstract：:Mn exerted various effects on the growth of Leptothrix discophora strain SS-1 in batch cultures depending on the concentration added to the medium. Concentrations of 0.55 to 5.5 muM Mn, comparable to those in the environment from which strain SS-1 was isolated, decreased cell yield and prolonged stationary-phase survi...

journal_title：Applied and environmental microbiology

authors： Adams LF,Ghiorse WC

更新日期：1985-03-01 00:00:00

abstract：:A greenhouse experiment was conducted to determine the influence of two tropical isolates of Glomus fasciculatum and Glomus mosseae on the nutrient uptake and growth of Sesbania grandiflora. Inoculation of sterile soil with the fungi significantly improved growth and nutrient uptake by S. grandiflora, but the response...

journal_title：Applied and environmental microbiology

authors： Habte M,Aziz T

更新日期：1985-09-01 00:00:00

abstract：:Salmonella is estimated to cause one million foodborne illnesses in the United States every year. Salmonella-contaminated poultry products are one of the major sources of salmonellosis. Given the critical role of the gut microbiota in Salmonella transmission, a manipulation of the chicken intestinal microenvironment c...

journal_title：Applied and environmental microbiology

authors： Azcarate-Peril MA,Butz N,Cadenas MB,Koci M,Ballou A,Mendoza M,Ali R,Hassan H

更新日期：2018-02-14 00:00:00

abstract：:In our previous study, Bacillus subtilis strain BSK3S, containing a polymyxin biosynthetic gene cluster from Paenibacillus polymyxa, could produce polymyxin only in the presence of exogenously added L-2,4-diaminobutyric acid (Dab). The dependence of polymyxin production on exogenous Dab was removed by introducing an e...

journal_title：Applied and environmental microbiology

authors： Park SY,Choi SK,Kim J,Oh TK,Park SH

更新日期：2012-06-01 00:00:00

abstract：:RNA was degraded at 60 degrees C for 24 h by halophilic nuclease H in supernatants from broth cultures of Micrococcus varians subsp. halophilus containing 12% NaCl. Since contaminating 5'-nucleotidase exhibited almost no activity under these conditions, the 5'-GMP formed could be recovered from the reaction mixture, a...

journal_title：Applied and environmental microbiology

authors： Kamekura M,Hamakawa T,Onishi H

更新日期：1982-10-01 00:00:00

abstract：:Excessive NH(3) production in the rumen is a major nutritional inefficiency in ruminant animals. Experiments were undertaken to compare the rates of NH(3) production from different substrates in ruminal fluid in vitro and to assess the role of asaccharolytic bacteria in NH(3) production. Ruminal fluid was taken from f...

journal_title：Applied and environmental microbiology

authors： Eschenlauer SC,McKain N,Walker ND,McEwan NR,Newbold CJ,Wallace RJ

更新日期：2002-10-01 00:00:00

abstract：:The biosynthesis of the major carotenoid spirilloxanthin by the purple nonsulfur bacterium Rhodospirillum rubrum is thought to occur via a linear pathway proceeding through phytoene and, later, lycopene as intermediates. This assumption is based solely on early chemical evidence (B. H. Davies, Biochem. J. 116:93-99, 1...

journal_title：Applied and environmental microbiology

authors： Wang GS,Grammel H,Abou-Aisha K,Sägesser R,Ghosh R

更新日期：2012-10-01 00:00:00

abstract：:We examined the competitive relationship between two isolates of denitrifying bacteria, both of which grow well under aerobic conditions but differ in their ability to grow under denitrifying conditions. The growth and persistence of the two isolates, added to sterile soil or added to soil previously colonized by the ...

journal_title：Applied and environmental microbiology

authors： Murray RE,Parsons LL,Smith MS

更新日期：1992-12-01 00:00:00

abstract：:From Bradyrhizobium japonicum highly reiterated sequence-possessing (HRS) strains indigenous to Niigata and Tokachi in Japan with high copy numbers of the repeated sequences RSalpha and RSbeta (K. Minamisawa, T. Isawa, Y. Nakatsuka, and N. Ichikawa, Appl. Environ. Microbiol. 64:1845-1851, 1998), several insertion sequ...

journal_title：Applied and environmental microbiology

authors： Isawa T,Sameshima R,Mitsui H,Minamisawa K

更新日期：1999-08-01 00:00:00

abstract：:To further study mechanisms of coping with osmotic stress-low water activity, mutants of Staphylococcus aureus with transposon Tn917-lacZ-induced NaCl sensitivity were selected for impaired ability to grow on solid defined medium containing 2 M NaCl. Southern hybridization experiments showed that NaCl-sensitive mutant...

journal_title：Applied and environmental microbiology

authors： Vijaranakul U,Nadakavukaren MJ,Bayles DO,Wilkinson BJ,Jayaswal RK

更新日期：1997-05-01 00:00:00

abstract：:An aseptic vacuum extraction technique was used to obtain xylem fluid from the roots of rough lemon (Citrus jambhiri Lush.) rootstock of Florida citrus trees. Bacteria were consistently isolated from vascular fluid of both healthy and young tree decline-affected trees. Thirteen genera of bacteria were found, the most ...

journal_title：Applied and environmental microbiology

authors： Gardner JM,Feldman AW,Zablotowicz RM

更新日期：1982-06-01 00:00:00

abstract：:A number of Micromonospora strains isolated from the water column, sediment, and cellulose baits placed in freshwater lakes were shown to be able to degrade cellulose in lake water without any addition of nutrients. A selective isolation method was also developed to demonstrate that CFU arose from both spores and hyph...

journal_title：Applied and environmental microbiology

authors： de Menezes AB,Lockhart RJ,Cox MJ,Allison HE,McCarthy AJ

更新日期：2008-11-01 00:00:00

abstract：:The ultrasonic determination of microbial population was carried out by using a new apparatus composed of two piezoelectric membranes. When the apparatus was immersed in a medium containing cells and an arbitrary voltage was charged on a piezoelectric membrane, an output voltage was obtained from the other membrane. T...

journal_title：Applied and environmental microbiology

authors： Ishimori Y,Karube I,Suzuki S

更新日期：1981-10-01 00:00:00

abstract：:In samples of flooded soil containing blue-green algae (cyanobacteria), the presence of rice plants did not influence the nitrogenase activity of the algae. Nitrogenase activity of heterotrophic bacteria was enhanced by the presence of rice plants, but this activity was not affected by changes in plant density. The ra...

journal_title：Applied and environmental microbiology

authors： Habte M,Alexander M

更新日期：1980-09-01 00:00:00