Homologous expression of recombinant manganese peroxidase in Phanerochaete chrysosporium.

abstract：:Three soilborne viruses transmitted by Polymyxa betae KESKIN in sugar beet have been described: Beet necrotic yellow vein virus (BNYVV), the agent of rhizomania, Beet soilborne virus (BSBV), and Beet virus Q (BVQ). A multiplex reverse transcription-PCR technique was developed to simultaneously detect BNYVV, BSBV, and ...

journal_title：Applied and environmental microbiology

authors： Meunier A,Schmit JF,Stas A,Kutluk N,Bragard C

更新日期：2003-04-01 00:00:00

abstract：:Hot and cold aqueous extracts were prepared from 22 commonly ingested fruits, vegetables, and seeds. When tested by agar diffusion, extracts from 13 and 10 of the foods formed precipitin bands with samples of normal rabbit serum and human saliva, respectively; extracts from four of the foods also reacted with antigen ...

journal_title：Applied and environmental microbiology

authors： Gibbons RJ,Dankers I

更新日期：1981-04-01 00:00:00

abstract：:A total of 896 samples of retail fresh meats and poultry was assayed for Escherichia coli serogroup O157:H7 by a hydrophobic grid membrane filter-immunoblot procedure developed specifically to isolate the organism from foods. The procedure involves several steps, including selective enrichment, filtration of enrichmen...

journal_title：Applied and environmental microbiology

authors： Doyle MP,Schoeni JL

更新日期：1987-10-01 00:00:00

abstract：:The membrane integrity of a cell is a well-accepted criterion for characterizing viable (active or inactive) cells and distinguishing them from damaged and membrane-compromised cells. This information is of major importance in studies of the function of microbial assemblages in natural environments, in order to assign...

journal_title：Applied and environmental microbiology

authors： Grégori G,Citterio S,Ghiani A,Labra M,Sgorbati S,Brown S,Denis M

更新日期：2001-10-01 00:00:00

abstract：:Lantibiotics are ribosomally synthesized antimicrobial peptides with substantial posttranslational modifications. They are characterized by the unique amino acids lanthionine and methyllanthionine, which are introduced by dehydration of Ser/Thr residues and linkage of the resulting dehydrated amino acids with Cys resi...

journal_title：Applied and environmental microbiology

authors： Basi-Chipalu S,Dischinger J,Josten M,Szekat C,Zweynert A,Sahl HG,Bierbaum G

更新日期：2015-05-15 00:00:00

abstract：:The transmission of Escherichia coli O157:H7 from manure-contaminated soil and irrigation water to lettuce plants was demonstrated using laser scanning confocal microscopy, epifluorescence microscopy, and recovery of viable cells from the inner tissues of plants. E. coli O157:H7 migrated to internal locations in plant...

journal_title：Applied and environmental microbiology

authors： Solomon EB,Yaron S,Matthews KR

更新日期：2002-01-01 00:00:00

abstract：:Microcosms capable of reductive dechlorination of polychlorinated dibenzo-p-dioxins/dibenzofurans (PCDD/Fs) were constructed in glass bottles by seeding them with a polluted river sediment and incubating them anaerobically with an organic medium. All of the PCDD/F congeners detected were equally reduced without the ac...

journal_title：Applied and environmental microbiology

authors： Yoshida N,Takahashi N,Hiraishi A

更新日期：2005-08-01 00:00:00

abstract：:Investigations concerning the role of distribution system biofilms on water quality were conducted at a drinking water utility in New Jersey. The utility experienced long-term bacteriological problems in the distribution system, while treatment plant effluents were uniformly negative for coliform bacteria. Results of ...

journal_title：Applied and environmental microbiology

authors： LeChevallier MW,Babcock TM,Lee RG

更新日期：1987-12-01 00:00:00

abstract：:Ticks are important vectors for many emerging pathogens. However, they are also infected with many symbionts and commensals, often competing for the same niches. In this paper, we characterize the microbiome of Amblyomma americanum (Acari: Ixodidae), the lone star tick, in order to better understand the evolutionary r...

journal_title：Applied and environmental microbiology

authors： Ponnusamy L,Gonzalez A,Van Treuren W,Weiss S,Parobek CM,Juliano JJ,Knight R,Roe RM,Apperson CS,Meshnick SR

更新日期：2014-01-01 00:00:00

abstract：:A novel S-adenosyl-L-methionine (SAM)-dependent methyltransferase catalyzing the O methylation of several chlorophenols and other halogenated phenols was purified 220-fold to apparent homogeneity from mycelia of Trichoderma longibrachiatum CECT 20431. The enzyme could be identified in partially purified protein prepar...

journal_title：Applied and environmental microbiology

authors： Coque JJ,Alvarez-Rodríguez ML,Larriba G

更新日期：2003-09-01 00:00:00

abstract：:Listeria monocytogenes can cause the serious infection listeriosis, which despite antibiotic treatment has a high mortality. Understanding the response of L. monocytogenes to antibiotic exposure is therefore important to ensure treatment success. Some bacteria survive antibiotic treatment by formation of persisters, w...

journal_title：Applied and environmental microbiology

authors： Knudsen GM,Ng Y,Gram L

更新日期：2013-12-01 00:00:00

abstract：:Respiratory denitrification is not always adequately established when bacteria are characterized. We have tested a simple method that allows one to evaluate whether the two necessary criteria to claim denitrification have been met, namely, that N(inf2) or N(inf2)O is produced from nitrate or nitrite and that this redu...

journal_title：Applied and environmental microbiology

authors： Mahne I,Tiedje JM

更新日期：1995-03-01 00:00:00

abstract：:Elevated winter losses of managed honeybee colonies are a major concern, but the underlying mechanisms remain controversial. Among the suspects are the parasitic mite Varroa destructor, the microsporidian Nosema ceranae, and associated viruses. Here we hypothesize that pathogens reduce the life expectancy of winter be...

journal_title：Applied and environmental microbiology

authors： Dainat B,Evans JD,Chen YP,Gauthier L,Neumann P

更新日期：2012-02-01 00:00:00

abstract：:Synthesis of the ligninolytic system of the wood-degrading fungus Phanerochaete chrysosporium is induced during secondary metabolism, brought about by nitrogen, carbon, or sulfur starvation. We describe here a strategy for selection of mutants which are ligninolytic (lignin----CO2) and overproduce lignin-degrading enz...

journal_title：Applied and environmental microbiology

authors： Tien M,Myer SB

更新日期：1990-08-01 00:00:00

abstract：:We previously published a genetic map of Gibberella zeae (Fusarium graminearum sensu lato) based on a cross between Kansas strain Z-3639 (lineage 7) and Japanese strain R-5470 (lineage 6). In this study, that genetic map was aligned with the third assembly of the genomic sequence of G. zeae strain PH-1 (lineage 7) usi...

journal_title：Applied and environmental microbiology

authors： Lee J,Jurgenson JE,Leslie JF,Bowden RL

更新日期：2008-04-01 00:00:00

abstract：:We have isolated and characterized the cDNA and genomic DNA coding for a phenoloxidase, laccase I, previously purified from culture supernatant of the newly isolated ligninolytic basidiomycete PM1 (CECT 2971). A cDNA library from basidiomycete PM1 was constructed, and laccase-encoding cDNAs were identified by screenin...

journal_title：Applied and environmental microbiology

authors： Coll PM,Tabernero C,Santamaría R,Pérez P

更新日期：1993-12-01 00:00:00

abstract：:A novel genomic island (LGI1) was discovered in Listeria monocytogenes isolates responsible for the deadliest listeriosis outbreak in Canada, in 2008. To investigate the functional role of LGI1, the outbreak strain 08-5578 was exposed to food chain-relevant stresses, and the expression of 16 LGI1 genes was measured. L...

journal_title：Applied and environmental microbiology

authors： Kovacevic J,Ziegler J,Wałecka-Zacharska E,Reimer A,Kitts DD,Gilmour MW

更新日期：2015-11-20 00:00:00

abstract：:Murine norovirus (MNV) was used as a surrogate to study resistance of human norovirus to disinfectants used in hospitals. MNV was sensitive to alcohol, alcohol hand rubs, bleach, and povidone iodine-based disinfectant. Real-time reverse transcription-PCR results indicated that the presence of viral RNA did not correla...

journal_title：Applied and environmental microbiology

authors： Belliot G,Lavaux A,Souihel D,Agnello D,Pothier P

更新日期：2008-05-01 00:00:00

abstract：:The bifidobacterial and lactobacillus populations of fecal samples collected from 10 human subjects were studied. The numbers of bifidobacteria were similar in the fecal samples of all of the subjects, but lactobacillus numbers varied, even between samples collected from the same individual. Analysis of the compositio...

journal_title：Applied and environmental microbiology

authors： Kimura K,McCartney AL,McConnell MA,Tannock GW

更新日期：1997-09-01 00:00:00

abstract：:Cattle are a known reservoir of Shiga toxin-producing Escherichia coli. The prevalence and stability of Shiga toxin and/or Shiga toxin genes among native wild ungulates in Idaho were investigated. The frequency of both Shiga genes and toxin was similar to that reported for Idaho cattle ( approximately 19%). ...

journal_title：Applied and environmental microbiology

authors： Gilbreath JJ,Shields MS,Smith RL,Farrell LD,Sheridan PP,Spiegel KM

更新日期：2009-02-01 00:00:00

abstract：:Human norovirus (NoV) is the leading cause of foodborne disease in the United States, and epidemiological studies have shown that fresh produce is one of the major vehicles for the transmission of human NoV. However, the mechanisms of norovirus contamination and persistence in fresh produce are poorly understood. The ...

journal_title：Applied and environmental microbiology

authors： DiCaprio E,Culbertson D,Li J

更新日期：2015-04-01 00:00:00

abstract：:A fixed-bed loop, high-rate anaerobic bioreactor treating sulfite evaporator condensate was sampled when it reached steady state and afterwards following perturbations during a 14-month period. By using immunotechnology, it was observed that shifts in methanogenic subpopulations occurred in association with perturbati...

journal_title：Applied and environmental microbiology

authors： Macario AJ,Conway de Macario E,Ney U,Schoberth SM,Sahm H

更新日期：1989-08-01 00:00:00

abstract：:Seasonal variation in the phylogenetic composition of Synechococcus assemblages in estuarine and coastal waters of Hong Kong was examined through pyrosequencing of the rpoC1 gene. Sixteen samples were collected in 2009 from two stations representing estuarine and ocean-influenced coastal waters, respectively. Synechoc...

journal_title：Applied and environmental microbiology

authors： Xia X,Vidyarathna NK,Palenik B,Lee P,Liu H

更新日期：2015-11-01 00:00:00

abstract：:Analysis of a complete set of glutamate decarboxylase (gad) mutants of Listeria monocytogenes strain LO28 (ΔgadD1, ΔgadDT1, ΔgadD2, ΔgadT2, and ΔgadD3 mutants) revealed that the ΔgadD1 mutant is impaired in its ability to tolerate exposure to both sublethal and lethal levels of the lantibiotic nisin. gadD1 is strain v...

journal_title：Applied and environmental microbiology

authors： Begley M,Cotter PD,Hill C,Ross RP

更新日期：2010-10-01 00:00:00

abstract：:Short-chain-length-medium-chain-length polyhydroxyalkanoates were synthesized in Saccharomyces cerevisiae from intermediates of the beta-oxidation cycle by expressing the polyhydroxyalkanoate synthases from Aeromonas caviae and Ralstonia eutropha in the peroxisomes. The quantity of polymer produced was increased by us...

journal_title：Applied and environmental microbiology

authors： De Oliveira VC,Maeda I,Delessert S,Poirier Y

更新日期：2004-09-01 00:00:00

abstract：:Fourteen different broth media were autoclaved under anaerobic conditions and then exposed to atmospheric oxygen. The hydrogen peroxide and superoxide radical formation as well as the bactericidal effect of the media were studied. The rate of killing of Peptostreptococcus anaerobius VPI 4330-1 was high in media that r...

journal_title：Applied and environmental microbiology

authors： Carlsson J,Nyberg G,Wrethén J

更新日期：1978-08-01 00:00:00

abstract：:To secure food and water safety, quantitative information on multiple pathogens is important. In this study, we developed a microfluidic quantitative PCR (MFQPCR) system to simultaneously quantify 11 major human viral pathogens, including adenovirus, Aichi virus, astrovirus, enterovirus, human norovirus, rotavirus, sa...

journal_title：Applied and environmental microbiology

authors： Ishii S,Kitamura G,Segawa T,Kobayashi A,Miura T,Sano D,Okabe S

更新日期：2014-12-01 00:00:00

abstract：:The effects of environmental stress exposure on Listeria monocytogenes growth and virulence-associated characteristics were investigated. Specifically, we measured the effects of temperature (7 or 37 degrees C), pH (5.5 or 7.4), the presence of salt and organic acids (375 mM NaCl, 8.45 mM sodium diacetate [SD], 275 mM...

journal_title：Applied and environmental microbiology

authors： Garner MR,James KE,Callahan MC,Wiedmann M,Boor KJ

更新日期：2006-08-01 00:00:00

abstract：:Sporulation of Bacillus larvae NRRL B-3650 occurred only at aeration rates lower than those used for cultivation of most Bacillus species. One possible explanation for the requirement for a low level of aeration in B. larvae is that toxic forms of oxygen such as H(2)O(2) and superoxide are involved. The superoxide dis...

journal_title：Applied and environmental microbiology

authors： Dingman DW,Stahly DP

更新日期：1984-06-01 00:00:00

abstract：:Rhizomucor miehei lipase (RML), as a kind of eukaryotic protein catalyst, plays an important role in the food, organic chemical, and biofuel industries. However, RML retains its catalytic activity below 50°C, which limits its industrial applications at higher temperatures. Soluble expression of this eukaryotic protein...

journal_title：Applied and environmental microbiology

authors： Li G,Fang X,Su F,Chen Y,Xu L,Yan Y

更新日期：2018-01-02 00:00:00