Glutamate decarboxylase-mediated nisin resistance in Listeria monocytogenes.

Abstract:

:Analysis of a complete set of glutamate decarboxylase (gad) mutants of Listeria monocytogenes strain LO28 (ΔgadD1, ΔgadDT1, ΔgadD2, ΔgadT2, and ΔgadD3 mutants) revealed that the ΔgadD1 mutant is impaired in its ability to tolerate exposure to both sublethal and lethal levels of the lantibiotic nisin. gadD1 is strain variable and is found only in approximately 50% of L. monocytogenes strains. Growth and survival experiments revealed that possession of gadD1 correlates with a higher degree of tolerance to nisin. Significantly, a similar finding using a gadB mutant of L. lactis IL1403 implies that this may be a general phenomenon in Gram-positive bacteria. Our findings thus suggest that the specific inhibition of GAD activity or a reduction in the levels of free glutamate may prevent the growth of otherwise resistant GAD(+) bacteria in foods where low pH and/or nisin is used as a preservative.

journal_name

Appl Environ Microbiol

authors

Begley M,Cotter PD,Hill C,Ross RP

doi

10.1128/AEM.00203-10

subject

Has Abstract

pub_date

2010-10-01 00:00:00

pages

6541-6

issue

19

eissn

0099-2240

issn

1098-5336

pii

AEM.00203-10

journal_volume

76

pub_type

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