Microfluidic quantitative PCR for simultaneous quantification of multiple viruses in environmental water samples.

abstract：:A triplex reverse transcriptase PCR (RT-PCR) was developed to simultaneously detect poliovirus, hepatitis A virus (HAV), and rotavirus in sewage and ocean water. Sewage and ocean water samples seeded with the three different viruses were concentrated by ultrafiltration. The unseeded ocean water and sewage samples were...

journal_title：Applied and environmental microbiology

authors： Tsai YL,Tran B,Sangermano LR,Palmer CJ

更新日期：1994-07-01 00:00:00

abstract：:The spirochete Borrelia burgdorferi is the causative agent of Lyme disease, the leading vector-borne illness in the United States. Many of the genetic factors affecting spirochete morphology and physiology are unknown due to the limited genetic tools available and the large number of open reading frames with unknown f...

journal_title：Applied and environmental microbiology

authors： Stewart PE,Hoff J,Fischer E,Krum JG,Rosa PA

更新日期：2004-10-01 00:00:00

abstract：:The wood protection industry has refined their products from chrome-, copper-, and arsenate-based wood preservatives toward solely copper-based preservatives in combination with organic biocides. One of these is Cu-HDO, containing the chelation product of copper and N-cyclohexyldiazenium dioxide (HDO). In this study, ...

journal_title：Applied and environmental microbiology

authors： Jakobs-Schönwandt D,Mathies H,Abraham WR,Pritzkow W,Stephan I,Noll M

更新日期：2010-12-01 00:00:00

abstract：:We developed a PCR detection method that selectively recognizes a single biological control agent and demonstrated that universally primed PCR (UP-PCR) can identify strain-specific markers. Antagonistic strains of Clonostachys rosea (syn. Gliocladium roseum) were screened by UP-PCR, and a strain-specific marker was id...

journal_title：Applied and environmental microbiology

authors： Bulat SA,Lübeck M,Alekhina IA,Jensen DF,Knudsen IM,Lübeck PS

更新日期：2000-11-01 00:00:00

abstract：:The fungus Cladosporium sphaerospermum was isolated from a biofilter used for the removal of toluene from waste gases. This is the first report describing growth of a eukaryotic organism with toluene as the sole source of carbon and energy. The oxygen consumption rates, as well as the measured enzyme activities, of to...

journal_title：Applied and environmental microbiology

authors： Weber FJ,Hage KC,de Bont JA

更新日期：1995-10-01 00:00:00

abstract：:Production of unconventional oil and gas continues to rise, but the effects of high-density hydraulic fracturing (HF) activity near aquatic ecosystems are not fully understood. A commonly used biocide in HF, 2,2-dibromo-3-nitrilopropionamide (DBNPA), was studied in microcosms of HF-impacted (HF+) versus HF-unimpacted ...

journal_title：Applied and environmental microbiology

authors： Campa MF,Techtmann SM,Ladd MP,Yan J,Patterson M,Garcia de Matos Amaral A,Carter KE,Ulrich N,Grant CJ,Hettich RL,Lamendella R,Hazen TC

更新日期：2019-10-16 00:00:00

abstract：:A fragment of about 500 base pairs of the beta-hemolysin gene from Listeria monocytogenes was used to screen different bacterial strains by DNA colony hybridization. The cells in the colonies were lysed by microwaves in the presence of sodium hydroxide. Of 52 different strains of Listeria species screened, only the DN...

journal_title：Applied and environmental microbiology

authors： Datta AR,Wentz BA,Hill WE

更新日期：1987-09-01 00:00:00

abstract：:Ammonia oxidation is a major process in nitrogen cycling, and it plays a key role in nitrogen limited soil ecosystems such as those in the arctic. Although mm-scale spatial dependency of ammonia oxidizers has been investigated, little is known about the field-scale spatial dependency of aerobic ammonia oxidation proce...

journal_title：Applied and environmental microbiology

authors： Banerjee S,Siciliano SD

更新日期：2012-01-01 00:00:00

abstract：:Small streams and their headwaters are key sources of microbial diversity in fluvial systems and serve as an entry point for bacteria from surrounding environments. Community assembly processes occurring in these streams shape downstream population structure and nutrient cycles. To elucidate the development and stabil...

journal_title：Applied and environmental microbiology

authors： Teachey ME,McDonald JM,Ottesen EA

更新日期：2019-05-16 00:00:00

abstract：:An immunolatex reagent was developed from antiserum to Fusobacterium varium, an obligate anaerobe isolated from the colon of the cockroach, Periplaneta americana L. A new technique that enabled the preparation of a highly efficient immunolatex conjugate was used to localize the bacterium with scanning electron microsc...

journal_title：Applied and environmental microbiology

authors： Bracke JW,Markovetz AJ

更新日期：1978-01-01 00:00:00

abstract：:Trypticase soy agar supplemented with sucrose, sodium chloride, bile salts, and triphenyltetrazolium chloride is an improved plating medium for the isolation of Vibrio parahaemolyticus from samples of seawater, permitting better differentiation of this organism from Vibrio alginolyticus and other bacteria. ...

journal_title：Applied and environmental microbiology

authors： Kourany M

更新日期：1983-01-01 00:00:00

abstract：:Knowledge of the mechanism of pressure-induced inactivation of microorganisms could be helpful in defining an effective, relatively mild pressure treatment as a means of decontamination, especially in combination with other physical treatments or antimicrobial agents. We have studied the effect of high pressure on Lac...

journal_title：Applied and environmental microbiology

authors： Wouters PC,Glaasker E,Smelt JP

更新日期：1998-02-01 00:00:00

abstract：:The culturability of Vibrio cholerae O1 serotype Inaba strain 569B was decreased by the addition of glucose to cell suspensions in starvation media. A similar effect was observed with sucrose, maltose, and fructose. We term this inhibitory effect glucose shock. It was not observed with arabinose or xylose or with carb...

journal_title：Applied and environmental microbiology

authors： Shiba T,Hill RT,Straube WL,Colwell RR

更新日期：1995-07-01 00:00:00

abstract：:Sulfolobus solfataricus is an aerobic crenarchaeal hyperthermophile with optimum growth at temperatures greater than 80°C and pH 2 to 4. Within the crenarchaeal group of Sulfolobales, N-acetylglucosamine (GlcNAc) has been shown to be a component of exopolysaccharides, forming their biofilms, and of the N-glycan decora...

journal_title：Applied and environmental microbiology

authors： Iacono R,Strazzulli A,Maurelli L,Curci N,Casillo A,Corsaro MM,Moracci M,Cobucci-Ponzano B

更新日期：2019-01-09 00:00:00

abstract：:Shewanella oneidensis couples oxidation of lactate to respiration of many substrates. Here we report that llpR (L-lactate-positive regulator, SO_3460) encodes a positive regulator of L-lactate utilization distinct from previously studied regulators. We also demonstrate D-lactate inhibition of L-lactate utilization in ...

journal_title：Applied and environmental microbiology

authors： Brutinel ED,Gralnick JA

更新日期：2012-12-01 00:00:00

abstract：:In the vast number of random mutagenesis experiments that have targeted protein thermostability, single amino acid substitutions that increase the apparent melting temperature (Tm) of the enzyme more than 1 to 2 degrees C are rare and often require the creation of a large library of mutated genes. Here we present a ca...

journal_title：Applied and environmental microbiology

authors： Xiao Z,Bergeron H,Grosse S,Beauchemin M,Garron ML,Shaya D,Sulea T,Cygler M,Lau PC

更新日期：2008-02-01 00:00:00

abstract：:Nitrogen-fixing symbiosis between bacteria and the tree legume mesquite (Prosopis glandulosa) is important for the maintenance of many desert ecosystems. Genes essential for nodulation and for extending the host range to mesquite were isolated from cosmid libraries of Rhizobium (mesquite) sp. strain HW17b and Bradyrhi...

journal_title：Applied and environmental microbiology

authors： Thomas PM,Golly KF,Virginia RA,Zyskind JW

更新日期：1995-09-01 00:00:00

abstract：:Enterohemorrhagic Escherichia coli O157:H7 has a natural reservoir in the intestinal tracts of cattle. Colonization is asymptomatic and transient, but it is not clear if protective immunity is induced. This study demonstrates that prior colonization induces humoral immune responses to bacterial antigens and reduces ba...

journal_title：Applied and environmental microbiology

authors： Naylor SW,Flockhart A,Nart P,Smith DG,Huntley J,Gally DL,Low JC

更新日期：2007-06-01 00:00:00

abstract：:The plasmid-free strain Lactococcus lactis subsp. cremoris BC101 produced an extracellular proteinase physicochemically similar to the proteinase encoded by the plasmid-linked prtP gene of other lactococcal strains. The absence of detectable plasmids in strain BC101 indicated that the prtP proteinase gene may be chrom...

journal_title：Applied and environmental microbiology

authors： Nissen-Meyer J,Lillehaug D,Nes IF

更新日期：1992-02-01 00:00:00

abstract：:In this work, a strain of anaerobic pentachlorophenol (PCP) degrader, Desulfitobacterium frappieri PCP-1, was used to augment a mixed bacterial community of an anaerobic upflow sludge bed reactor degrading PCP. To estimate the efficiency of augmentation, the population of PCP-1 in the reactor was enumerated by a compe...

journal_title：Applied and environmental microbiology

authors： Tartakovsky B,Levesque M,Dumortier R,Beaudet R,Guiot SR

更新日期：1999-10-01 00:00:00

abstract：:Marine viruses are an important component of the microbial food web, influencing microbial diversity and contributing to bacterial mortality rates. Resistance to cooccurring cyanophages has been reported for natural communities of Synechococcus spp.; however, little is known about the nature of this resistance. This s...

journal_title：Applied and environmental microbiology

authors： Stoddard LI,Martiny JB,Marston MF

更新日期：2007-09-01 00:00:00

abstract：:Although carbon dioxide (CO(2)) is known to inhibit growth of most bacteria, very little is known about the cellular response. The food-borne pathogen Listeria monocytogenes is characterized by its ability to grow in high CO(2) concentrations at refrigeration temperatures. We examined the listerial responses of differ...

journal_title：Applied and environmental microbiology

authors： Jydegaard-Axelsen AM,Høiby PE,Holmstrøm K,Russell N,Knøchel S

更新日期：2004-07-01 00:00:00

abstract：:Pullulanase (EC 3.2.1.41), an important enzyme in the production of starch syrup, catalyzes the hydrolysis of α-1,6 glycosidic bonds in complex carbohydrates. A double mutant (DM; D437H/D503Y) form of Bacillus deramificans pullulanase was recently constructed to enhance the thermostability and catalytic efficiency of ...

journal_title：Applied and environmental microbiology

authors： Duan X,Wu J

更新日期：2015-03-01 00:00:00

abstract：:Accurate enumeration of viruses within environmental samples is critical for investigations of the ecological role of viruses and viral infection within microbial communities. This report evaluates differences in viral and bacterial direct counts between estuarine sediment samples which were either immediately process...

journal_title：Applied and environmental microbiology

authors： Helton RR,Liu L,Wommack KE

更新日期：2006-07-01 00:00:00

abstract：:The capacity of Azospirillum brasilense to enhance the accumulation of K, P, Ca, Mg, S, Na, Mn, Fe, B, Cu, and Zn in inoculated wheat and soybean plants was evaluated by using two different analytical methods with five A. brasilense strains originating from four distinct geographical regions. A Pseudomonas isolate fro...

journal_title：Applied and environmental microbiology

authors： Bashan Y,Harrison SK,Whitmoyer RE

更新日期：1990-03-01 00:00:00

abstract：:Serratia marcescens incubated for 8 h at 31 degrees C in a chemically defined medium contained in shake flasks was aerosolized into rotating-drum aerosol chambers at 30 degrees C and saturated humidity. Cells furnished tryptone (Difco) and glycerol just before aerosolization increased (in viable numbers and countable ...

journal_title：Applied and environmental microbiology

authors： Dimmick RL,Wolochow H,Chatigny MA

更新日期：1979-05-01 00:00:00

abstract：:Highly methylated, long-chain celluloses strongly inhibited cellulose degradation by several species of cellulolytic bacteria of ruminal origin. Specifically, the inhibitory effects of methylcellulose on the growth of Ruminococcus flavefaciens FD1 were concentration dependent, with complete inhibition at 0.1% (wt/vol)...

journal_title：Applied and environmental microbiology

authors： Rasmussen MA,Hespell RB,White BA,Bothast RJ

更新日期：1988-04-01 00:00:00

abstract：:This study was aimed at assessing whether the repeated exposure of 12 strains of Salmonella spp., Escherichia coli, and Listeria monocytogenes to alternative nonthermal decontamination techniques with UV light (UV-C) and nonthermal atmospheric plasma (NTAP) may cause the emergence of variants showing increased resista...

journal_title：Applied and environmental microbiology

authors： Álvarez-Molina A,de Toro M,Ruiz L,López M,Prieto M,Alvarez-Ordóñez A

更新日期：2020-04-17 00:00:00

abstract：:Saturation point mutagenesis was carried out at position 479 in the polyhydroxyalkanoate (PHA) synthase from Chromobacterium sp. strain USM2 (PhaC(Cs)) with specificities for short-chain-length (SCL) [(R)-3-hydroxybutyrate (3HB) and (R)-3-hydroxyvalerate (3HV)] and medium-chain-length (MCL) [(R)-3-hydroxyhexanoate (3H...

journal_title：Applied and environmental microbiology

authors： Chuah JA,Tomizawa S,Yamada M,Tsuge T,Doi Y,Sudesh K,Numata K

更新日期：2013-06-01 00:00:00

abstract：:The Tinto River (Huelva, southwestern Spain) is an extreme environment with a rather constant acidic pH along the entire river and a high concentration of heavy metals. The extreme conditions of the Tinto ecosystem are generated by the metabolic activity of chemolithotrophic microorganisms thriving in the rich complex...

journal_title：Applied and environmental microbiology

authors： González-Toril E,Llobet-Brossa E,Casamayor EO,Amann R,Amils R

更新日期：2003-08-01 00:00:00